Journal: Frontiers in Endocrinology

Article Title: Integrative bioinformatics analysis identifies placental senescence-associated signatures in early-onset preeclampsia

doi: 10.3389/fendo.2026.1863608

Figure Lengend Snippet: Experimental validation supports a senescence-associated role of LEP in EOPE. (A) qRT–PCR analysis of LEP, ENG, MIF and CYBB mRNA expression in PTB and EOPE placentas. n = 6 biologically independent placentas per group. Bars represent mean ± SD. (B) Representative images of senescence-associated β-galactosidase (SA-β-gal) staining. (C) Representative immunofluorescence images of P21 in PTB and EOPE placentas. Nuclei were counterstained with Hoechst. (D) Representative images of LEP immunohistochemistry in PTB and EOPE placentas. Scale bars, 100 μm. n = 6 biologically independent placentas per group. (E) Representative western blot showing LEP protein expression in PTB and EOPE placentas. n = 6. (F) Western blot analysis of P21 and LEP protein expression in BeWo cells treated with increasing concentrations of leptin. (G) Representative SA-β-gal staining images (left) and quantification of SA-β-gal-positive cells (right) in BeWo cells treated with leptin. n = 3 biologically independent experiments. Bars represent mean ± SD. (H) Detection of hCG in cell culture supernatant by ELISA after 24 h of leptin treatment.

Article Snippet: The obtained placental tissue sections were incubated overnight at 4 °C in a humidified incubation chamber with the primary antibody: anti-rabbit leptin (A1300, ABclonal).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, Staining, Immunofluorescence, Immunohistochemistry, Western Blot, Cell Culture, Enzyme-linked Immunosorbent Assay