Journal: Nature Communications

Article Title: Adipocyte OGT governs diet-induced hyperphagia and obesity

doi: 10.1038/s41467-018-07461-x

Figure Lengend Snippet: Adipocyte OGT drives diet-induced hyperphagia and obesity. a Genetic cross scheme. b Growth curves and c body composition of adipocyte-specific OGT knockout mice (FKO) and littermate control mice (Con) on chow ( n = 8 (Con, dashed line with empty square/stripped bar) or 11 (FKO, blue line/bar)) or HFD ( n = 11 (Con, dashed line with empty circle/empty bar) or 13 (FKO, red line/bar)). d Circulating levels of leptin and e wet tissue weights in male Con ( n = 6) and FKO ( n = 6) mice fed HFD for 9 weeks, or age-matched Con ( n = 8) and FKO ( n = 8) mice fed chow. BAT, interscapular brown adipose tissue. White adipose tissues include depots from perigonadal (pg), subcutaneous (sc), and retroperitoneal (rp) regions. f Energy intake of male mice on HFD ( n = 7 per genotype) or Chow ( n = 5 (Con) or n = 6 (FKO)) for 13 weeks. g Fecal energy content. h Daily energy intake. Male Con ( n = 10) and FKO ( n = 10) mice fed HFD for 6 weeks. i Energy expenditure of 5-week HFD-fed male Con ( n = 7) and FKO ( n = 9) mice. j Energy intake in a two-choice diet preference test. Male Con ( n = 9) and FKO ( n = 10) mice have been fed HFD for 7 or 16 weeks. k Growth curves of HFD-fed mice. Con (AL, ad libitum, n = 11), Con (PF, pair-fed to littermate FKO, n = 7), FKO (AL, n = 7). Data were presented as mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, post hoc Sidak’s tests ( b (HFD Con vs. HFD FKO), i , k (Con PF vs. FKO AL)), Tukey’s tests ( c , d , f ), or two-tailed Student’s t-test ( e , g , h , j )

Article Snippet: Recombinant mouse leptin protein (5 mg/kg, R&D Systems cat# 498-OB) was reconstituted at 1 mg/mL in sterile 20 mM Tris-HCl, pH 8.0.

Techniques: Knock-Out, Control, Two Tailed Test

Journal: Nature Communications

Article Title: Adipocyte OGT governs diet-induced hyperphagia and obesity

doi: 10.1038/s41467-018-07461-x

Figure Lengend Snippet: Adipocyte OGT drives diet-induced hyperphagia through transactivation of lipid desaturation in adipose tissue. a Gene expression analysis of de novo lipid desaturation and synthesis in adipose tissue (Chow for 15 weeks, Con n = 8, FKO n = 8; age-matched HFD for 9 weeks, Con n = 5, FKO n = 6). b Protein levels of SCD and OGT in adipose tissue. c Western blotting analysis in adipose tissue from 9-week-HFD-fed FKO and Con mice (Con n = 5, FKO n = 6, two representative biological replicates were shown). d Growth curves and e energy intake of (m)HFD-fed Con and FKO mice ( n = 8–10 per group). mHFD denotes a mono-unsaturated fat-fortified high-fat diet that changes the saturated fat-rich soybean oil fraction into the mono-unsaturated fat-rich canola oil as illustrated in the diagram ( d ). f Contents of palmitate (C16:0) and palmitoleate (C16:1) DAG in adipose lipidome, and circulating levels of leptin ( g ), or of FGF21 ( h ) in mice fed HFD or mHFD for 15 weeks. Data were presented as mean ± s.e.m. n.s. not significant, * P < 0.05, ** P < 0.01, **** P < 0.0001, post hoc Sidak’s tests ( a , e – h ), or # P < 0.01 HFD Con vs. HFD FKO, two-tailed Student’s t-test ( a ). A dashed line in ( e – h ) indicates that data from HFD study and mHFD study are presented in a side-by-side manner, and inference may not be made between different diets

Article Snippet: Recombinant mouse leptin protein (5 mg/kg, R&D Systems cat# 498-OB) was reconstituted at 1 mg/mL in sterile 20 mM Tris-HCl, pH 8.0.

Techniques: Gene Expression, Western Blot, Two Tailed Test

Journal: bioRxiv

Article Title: Adipose Tissue Inflammation, Oxidative Stress, and Altered Adipogenesis Are Drivers of Dyslipidemia – A Multi-Omics Overview of Dyslipidemia in Obesity

doi: 10.1101/2025.10.15.682716

Figure Lengend Snippet: Correlation plots of leptin with HDL-C and ApoAI with lines of best fit and confidence interval. Spearman’s rank correlation (r), and its Benjamini-Hochberg adjusted p-value ( p ), only significant correlations depicted.

Article Snippet: Plasma adiponectin and leptin concentrations were measured using human Adiponectin/Acrp30 DuoSet ® ELISA (Y1065-05; R&D Systems, Minneapolis, USA) and human Leptin DuoSet ® ELISA (DY398-05; R&D Systems, Minneapolis, USA).

Techniques:

Journal: PLoS Genetics

Article Title: Congenital lipodystrophy induces severe osteosclerosis

doi: 10.1371/journal.pgen.1008244

Figure Lengend Snippet: A) Transplanted fat depots 3 months after surgery. B) Fat depot weight 3 months after transplantation. C) Serum leptin and adiponectin of FF mice 3 months after fat depot transplantation. WT and non-transplanted FF mice serve as control. D) μCT analysis of trabecular bone volume and bone mineral density of distal femurs of FF mice 3 months after sham operation or transplantation of fat derived from WT or adipokine-deficient mice. Data are presented as mean ± SD. **p<0.01; *** p<0.001 as determined by ANOVA with Holm-Sidak's post hoc analysis for multiple comparisons test. D) comparison with FF Sham except where detailed.

Article Snippet: The primary antibody cocktail contained rat anti-mouse CD45-BUV395 (BD Horizon, clone 30-F11, final dilution factor 1:200), rat anti-mouse TER-119-APC (BioLegend, clone TER-119, 1:200), rat anti-mouse CD41-BV421 (BioLegend, clone MWReg30, 1:300), rat anti-mouse/human CD11b (BioLegend, clone M1/70, 1:400), and rat-anti mouse Leptin receptor (LepR)-biotin (R&D Systems, polyclonal, 1:50) in Brilliant Stain Buffer (BD Biosciences) containing 10 μg/mL FcBlock.

Techniques: Transplantation Assay, Control, Derivative Assay, Comparison

Journal: Metabolites

Article Title: Effect of Metabolic and Bariatric Surgery Associated with Changes in Weight Loss, Free Leptin Index, and Soluble Leptin Receptor

doi: 10.3390/metabo15120764

Figure Lengend Snippet: Comparison of circulating leptin ( A ), FLI ( B ), sOB-R ( C ) and ghrelin ( D ) pre-surgery and post-surgery (week 1 until 12 months post-surgery). Statistically significant differences were observed in groups post-surgery, * p < 0.01; ** p < 0.001; *** p < 0.005; **** p < 0.0001.

Article Snippet: Leptin levels were quantified with the Human Leptin DuoSet ELISA (Catalog No. DY398-05, R&D Systems, Inc., Minneapolis, MN, USA) and sOB-R levels with the Human Leptin Receptor DuoSet ELISA (Catalog No. DY389, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques: Comparison

Journal: Metabolites

Article Title: Effect of Metabolic and Bariatric Surgery Associated with Changes in Weight Loss, Free Leptin Index, and Soluble Leptin Receptor

doi: 10.3390/metabo15120764

Figure Lengend Snippet: ( A ) Correlation matrix between circulating leptin, ghrelin, FLI, and anthropometric/metabolic variable changes following metabolic and bariatric surgery. Values are repeated-measures correlations (also called intra-individual correlations). Only significant results are shown based on Bonferroni-adjusted p -value. ( B ) Graph of correlations of sOB-R and FLI with leptin.

Article Snippet: Leptin levels were quantified with the Human Leptin DuoSet ELISA (Catalog No. DY398-05, R&D Systems, Inc., Minneapolis, MN, USA) and sOB-R levels with the Human Leptin Receptor DuoSet ELISA (Catalog No. DY389, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques: