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plenticrispr v2 vector  (Addgene inc)


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    Structured Review

    Addgene inc plenticrispr v2 vector
    Plenticrispr V2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lenticrispr+vector/lentiCRISPR+v2+(Plasmid+%2352961)/pm42844288-314-9-12
    Average 96 stars, based on 6191 article reviews
    plenticrispr v2 vector - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Increased mRNA translation delays lung adenocarcinoma initiation and exposes a therapeutic vulnerability to MEK inhibitors
    Article Snippet: For Western blots, the following antibodies were used at a dilution of 1:1000: eIF4A1 (abcam 31217), eIF4A2 (abcam 31218), pmTORC1 (Cell signalling 2971), pPERK (cell signalling 3179), ATF4 (Cell signalling 11815), pAKT (Cell signalling 3787), pERK1/2 (Cell signalling 9106), ERK1/2 (Sigma M5670) GAPDH (Sigma G8795), β-actin (Sigma A1978), p21 (Abcam ab107099), p16 (Abcam ab211542) .. Guide RNA (gRNA) sequences targeting Eif4a2 were cloned into a lentiCRISPR vector (Addgene 52961). gRNAs were as follows: eIF4A2 (forward) CACCGGAAGCCCCTCACATTGTTGT; eIF4A2 (reverse) AAACACAACAATGTGAGGGGCTTCC. ..

    Article Title: Uridine phosphorylase-1 supports metastasis by altering immune and extracellular matrix landscapes
    Article Snippet: .. Guide RNA (gRNA) sequences were designed to target Upp1 (mUPP1 Guide 1 F: CACCGCACCAACCTGGACGCGCAGC; mUPP1 Guide 1R: AAACGCTGCGCGTCCAGGTTGGTGC), and cloned into a lentiCRISPR vector (Addgene) (O’Prey et al, ; Shalem et al, ). ..

    Article Title: Metabolic adaptations of micrometastases alter EV production to generate invasive microenvironments.
    Article Snippet: .. Generation of nSMase and Rab27 CRISPR cells gRNA sequences targeting neutral sphingomyelinases 1 and 2 and Rab27s a and b were cloned into a lentiCRISPR vector (Addgene [52961]) (O’Prey et al., 2017; Shalem et al., 2014). gRNAs were as follows: Rab27a (forward) 59-CACCG CCAGGAGCATCTCAATCGCG-39; Rab27a (reverse) 59-AAACCGCGATTGAGATG CTCCTGGC-39; Rab27b (forward) 59-CACCGGCTGCGCTTGTTTCGAAGTA-39; Rab27b (reverse) 59-AAACTACTTCGAAACAAGCGCAGCC-39; neutral sphingomyelinase-1 (forward) 59-CACCGCGCCCTATGT TTGCTCAGGT-39; neutral sphingomyelinase-1 (reverse) 59-AAACACCTGAGCAAA CATAGGGCGC-39; neutral sphingomyelinase-2 (forward) 59-CACCGGTGGTGTTTG ACGTCATCTG-39; neutral sphingomyelinase-2 (reverse) 59-AAACCAGATGACGTC AAACACCACC-39. .. Then, to produce lentivirus-encoding Cas9, along with the desired gRNAs, HEK 293T cells were used as the host-packaging cell line (1 million cells were seeded overnight and transfected on the next day with 10 μg of plentiCRISPR for each of the gRNAs, 7.5 μg psPAX2, #12260; Addgene, and 4 μg pVSV-G, #138479; Addgene).

    Article Title: Metabolic adaptations of micrometastases alter EV production to generate invasive microenvironments
    Article Snippet: .. Guide RNA (gRNA) sequences targeting neutral sphingomyelinases 1 & 2 and Rab27s a and b were cloned into a lentiCRISPR vector (Addgene (52961) , . gRNAs were as follows: Rab27a (forward) CACCG CCAGGAGCATCTCAATCGCG; Rab27a (reverse) AAACCGCGATTGAGATG CTCCTGGC; Rab27b (forward) CACCGGCTGCGCTTGTTTCGAAGTA; Rab27b (reverse) AAACTACTTCGAAACAAGCGCAGCC; neutral sphingomyelinase-1 (forward) CACCGCGCCCTATGTTTGCTCAGGT; neutral sphingomyelinase-1 (reverse) AAACACCTGAGCAAACATAGGGCGC; neutral sphingomyelinase-2 (forward) CACCGGTGGTGTTTGACGTCATCTG; neutral sphingomyelinase-2 (reverse) AAACCAGATGACGTCAAACACCACC. ..

    Article Title: Metabolic adaptations of micrometastases alter EV production to generate invasive microenvironments
    Article Snippet: .. gRNA sequences targeting neutral sphingomyelinases 1 and 2 and Rab27s a and b were cloned into a lentiCRISPR vector (Addgene [52961]) ( ; ). gRNAs were as follows: Rab27a (forward) 5′-CACCG CCA GGA GCA TCT CAA TCG CG-3′; Rab27a (reverse) 5′-AAA CCG CGA TTG AGA TG CTCCTGGC-3′; Rab27b (forward) 5′-CAC CGG CTG CGC TTG TTT CGA AGT A-3′; Rab27b (reverse) 5′-AAA CTA CTT CGA AAC AAG CGC AGC C-3′; neutral sphingomyelinase-1 (forward) 5′-CAC CGC GCC CTA TGT TTG CTC AGG T-3′; neutral sphingomyelinase-1 (reverse) 5′-AAA CAC CTG AGC AAA CAT AGG GCG C-3′; neutral sphingomyelinase-2 (forward) 5′-CAC CGG TGG TGT TTG ACG TCA TCT G-3′; neutral sphingomyelinase-2 (reverse) 5′-AAA CCA GAT GAC GTC AAA CAC CAC C-3′. .. Then, to produce lentivirus-encoding Cas9, along with the desired gRNAs, HEK 293T cells were used as the host-packaging cell line (1 million cells were seeded overnight and transfected on the next day with 10 μg of plentiCRISPR for each of the gRNAs, 7.5 μg psPAX2, #12260; Addgene, and 4 μg pVSV-G, #138479; Addgene).

    Plasmid Preparation:

    Article Title: Increased mRNA translation delays lung adenocarcinoma initiation and exposes a therapeutic vulnerability to MEK inhibitors
    Article Snippet: For Western blots, the following antibodies were used at a dilution of 1:1000: eIF4A1 (abcam 31217), eIF4A2 (abcam 31218), pmTORC1 (Cell signalling 2971), pPERK (cell signalling 3179), ATF4 (Cell signalling 11815), pAKT (Cell signalling 3787), pERK1/2 (Cell signalling 9106), ERK1/2 (Sigma M5670) GAPDH (Sigma G8795), β-actin (Sigma A1978), p21 (Abcam ab107099), p16 (Abcam ab211542) .. Guide RNA (gRNA) sequences targeting Eif4a2 were cloned into a lentiCRISPR vector (Addgene 52961). gRNAs were as follows: eIF4A2 (forward) CACCGGAAGCCCCTCACATTGTTGT; eIF4A2 (reverse) AAACACAACAATGTGAGGGGCTTCC. ..

    Article Title: Uridine phosphorylase-1 supports metastasis by altering immune and extracellular matrix landscapes
    Article Snippet: .. Guide RNA (gRNA) sequences were designed to target Upp1 (mUPP1 Guide 1 F: CACCGCACCAACCTGGACGCGCAGC; mUPP1 Guide 1R: AAACGCTGCGCGTCCAGGTTGGTGC), and cloned into a lentiCRISPR vector (Addgene) (O’Prey et al, ; Shalem et al, ). ..

    Article Title: Metabolic adaptations of micrometastases alter EV production to generate invasive microenvironments.
    Article Snippet: .. Generation of nSMase and Rab27 CRISPR cells gRNA sequences targeting neutral sphingomyelinases 1 and 2 and Rab27s a and b were cloned into a lentiCRISPR vector (Addgene [52961]) (O’Prey et al., 2017; Shalem et al., 2014). gRNAs were as follows: Rab27a (forward) 59-CACCG CCAGGAGCATCTCAATCGCG-39; Rab27a (reverse) 59-AAACCGCGATTGAGATG CTCCTGGC-39; Rab27b (forward) 59-CACCGGCTGCGCTTGTTTCGAAGTA-39; Rab27b (reverse) 59-AAACTACTTCGAAACAAGCGCAGCC-39; neutral sphingomyelinase-1 (forward) 59-CACCGCGCCCTATGT TTGCTCAGGT-39; neutral sphingomyelinase-1 (reverse) 59-AAACACCTGAGCAAA CATAGGGCGC-39; neutral sphingomyelinase-2 (forward) 59-CACCGGTGGTGTTTG ACGTCATCTG-39; neutral sphingomyelinase-2 (reverse) 59-AAACCAGATGACGTC AAACACCACC-39. .. Then, to produce lentivirus-encoding Cas9, along with the desired gRNAs, HEK 293T cells were used as the host-packaging cell line (1 million cells were seeded overnight and transfected on the next day with 10 μg of plentiCRISPR for each of the gRNAs, 7.5 μg psPAX2, #12260; Addgene, and 4 μg pVSV-G, #138479; Addgene).

    Article Title: Uridine phosphorylase-1 supports metastasis by altering immune and extracellular matrix landscapes
    Article Snippet: Recombinant DNA , , . .. lentiCRISPR vector , Addgene , 52961. ..

    Article Title: Metabolic adaptations of micrometastases alter EV production to generate invasive microenvironments
    Article Snippet: .. Guide RNA (gRNA) sequences targeting neutral sphingomyelinases 1 & 2 and Rab27s a and b were cloned into a lentiCRISPR vector (Addgene (52961) , . gRNAs were as follows: Rab27a (forward) CACCG CCAGGAGCATCTCAATCGCG; Rab27a (reverse) AAACCGCGATTGAGATG CTCCTGGC; Rab27b (forward) CACCGGCTGCGCTTGTTTCGAAGTA; Rab27b (reverse) AAACTACTTCGAAACAAGCGCAGCC; neutral sphingomyelinase-1 (forward) CACCGCGCCCTATGTTTGCTCAGGT; neutral sphingomyelinase-1 (reverse) AAACACCTGAGCAAACATAGGGCGC; neutral sphingomyelinase-2 (forward) CACCGGTGGTGTTTGACGTCATCTG; neutral sphingomyelinase-2 (reverse) AAACCAGATGACGTCAAACACCACC. ..

    Article Title: Mifepristone protects acetaminophen induced liver injury through NRF2/GSH/GST mediated ferroptosis suppression.
    Article Snippet: Ferroptosis is a form of iron-dependent cell death that has attracted significant attention for its potential role in numerous diseases.. Targeted inhibition of ferroptosis could be of potential use in treating diseases: such as drug induced liver injury (DILI).. Ferroptosis can be antagonized by the xCT/GSH/GPX4, FSP1/CoQ10, DHODH/CoQ10, GCH1/BH4, and NRF2 pathways.

    Article Title: Metabolic adaptations of micrometastases alter EV production to generate invasive microenvironments
    Article Snippet: .. gRNA sequences targeting neutral sphingomyelinases 1 and 2 and Rab27s a and b were cloned into a lentiCRISPR vector (Addgene [52961]) ( ; ). gRNAs were as follows: Rab27a (forward) 5′-CACCG CCA GGA GCA TCT CAA TCG CG-3′; Rab27a (reverse) 5′-AAA CCG CGA TTG AGA TG CTCCTGGC-3′; Rab27b (forward) 5′-CAC CGG CTG CGC TTG TTT CGA AGT A-3′; Rab27b (reverse) 5′-AAA CTA CTT CGA AAC AAG CGC AGC C-3′; neutral sphingomyelinase-1 (forward) 5′-CAC CGC GCC CTA TGT TTG CTC AGG T-3′; neutral sphingomyelinase-1 (reverse) 5′-AAA CAC CTG AGC AAA CAT AGG GCG C-3′; neutral sphingomyelinase-2 (forward) 5′-CAC CGG TGG TGT TTG ACG TCA TCT G-3′; neutral sphingomyelinase-2 (reverse) 5′-AAA CCA GAT GAC GTC AAA CAC CAC C-3′. .. Then, to produce lentivirus-encoding Cas9, along with the desired gRNAs, HEK 293T cells were used as the host-packaging cell line (1 million cells were seeded overnight and transfected on the next day with 10 μg of plentiCRISPR for each of the gRNAs, 7.5 μg psPAX2, #12260; Addgene, and 4 μg pVSV-G, #138479; Addgene).

    CRISPR:

    Article Title: Metabolic adaptations of micrometastases alter EV production to generate invasive microenvironments.
    Article Snippet: .. Generation of nSMase and Rab27 CRISPR cells gRNA sequences targeting neutral sphingomyelinases 1 and 2 and Rab27s a and b were cloned into a lentiCRISPR vector (Addgene [52961]) (O’Prey et al., 2017; Shalem et al., 2014). gRNAs were as follows: Rab27a (forward) 59-CACCG CCAGGAGCATCTCAATCGCG-39; Rab27a (reverse) 59-AAACCGCGATTGAGATG CTCCTGGC-39; Rab27b (forward) 59-CACCGGCTGCGCTTGTTTCGAAGTA-39; Rab27b (reverse) 59-AAACTACTTCGAAACAAGCGCAGCC-39; neutral sphingomyelinase-1 (forward) 59-CACCGCGCCCTATGT TTGCTCAGGT-39; neutral sphingomyelinase-1 (reverse) 59-AAACACCTGAGCAAA CATAGGGCGC-39; neutral sphingomyelinase-2 (forward) 59-CACCGGTGGTGTTTG ACGTCATCTG-39; neutral sphingomyelinase-2 (reverse) 59-AAACCAGATGACGTC AAACACCACC-39. .. Then, to produce lentivirus-encoding Cas9, along with the desired gRNAs, HEK 293T cells were used as the host-packaging cell line (1 million cells were seeded overnight and transfected on the next day with 10 μg of plentiCRISPR for each of the gRNAs, 7.5 μg psPAX2, #12260; Addgene, and 4 μg pVSV-G, #138479; Addgene).

    Knock-Out:

    Article Title: Tuning Insulin Receptor Signaling Using De Novo Designed Agonists
    Article Snippet: Cells were selected with 2 mg/ml of puromycin at 3 days after infection and sorted using FACS sorter (Sony Ma900). .. To generate IGF1R knockout C2C12 cells expressing human IR-A (C2C12-IR), lentiviruses were packaged in 293FT cells by transfecting the cells with lentiCRISPR vector (Addgene # 52962, gRNA: CACCGCTATGGTGGAGAGGTAACAG), psPAX2 (Addgene #12260), and pMD2.G (Addgene #12259). ..

    Expressing:

    Article Title: Tuning Insulin Receptor Signaling Using De Novo Designed Agonists
    Article Snippet: Cells were selected with 2 mg/ml of puromycin at 3 days after infection and sorted using FACS sorter (Sony Ma900). .. To generate IGF1R knockout C2C12 cells expressing human IR-A (C2C12-IR), lentiviruses were packaged in 293FT cells by transfecting the cells with lentiCRISPR vector (Addgene # 52962, gRNA: CACCGCTATGGTGGAGAGGTAACAG), psPAX2 (Addgene #12260), and pMD2.G (Addgene #12259). ..



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