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cona lectin  (Vector Laboratories)


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    Vector Laboratories cona lectin
    a , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in vivo. WT mice under a regular 12 h light–dark cycle and NR feeding were subcutaneously injected with either vehicle (Veh) or CP-91149 at ZT0 for tissue collections at ZT3, ZT6, ZT9, ZT12 and ZT24. b , Average food consumption of WT mice ( n = 4 biological replicates) 24 h after CP-91149 injection. c , Temporal profiles of glycogen in the liver of Veh-injected and CP-91149-injected mice ( n = 20 (5 timepoints × 4 biological replicates)). d , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated mouse liver were determined by <t>lectin</t> blot analysis with concanavalin A <t>(ConA,</t> n = 20 (5 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (right). e , C3 levels in mouse serum as assessed by ELISA ( n = 20 (5 timepoints × 4 biological replicates)). f , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in AML12 mouse hepatocytes. Treatment with Veh or CP-91149 (67 µM) was performed for 3, 6, 12 and 24 h. g , h , Kinetic profiles of UDP-glucose + UDP-galactose levels ( g ), cytidine 5′-monophospho-N-acetyl neuraminic acid (CMP-Neu5Ac) ( h , left) and uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) ( h , right) in AML12 cells upon CP-91149 treatment ( n = 16 (4 timepoints × 4 biological replicates)). i , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated AML12 cells were determined by lectin blot analysis with ConA ( n = 16 (4 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (bottom). j , ALB, FN1 and C3 levels in cell medium determined by ELISA ( n = 22–24 (4 timepoints × 6 biological replicates, with ALB 14 h Veh and 24 h CP-91149 n = 5)). k , Experimental design (created with BioRender.com ). AML12 cells were treated with Veh or CP-91149 for 14 h in the absence or presence of supplemental UDP-glucose (UDPG; 2 mM). l , m , ALB and C3 levels in cell medium ( l ) and lysates ( m ) as determined by ELISA ( n = 6 biological replicates, except for ALB under CP-91149 treatment ( n = 5)). Data are displayed as means; error bars, s.e.m. Boxplots show the median (centre line), interquartile range (box) and minimum to maximum values (whiskers). A detailed description of the statistical analysis is available in Source Data Fig. . See also Extended Data Fig. .
    Cona Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lectins+cona/Biotinylated+Concanavalin+A+(Con+A)/pmc12945689-402-34-36
    Average 94 stars, based on 302 article reviews
    cona lectin - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Feeding-regulated glycogen metabolism drives rhythmic liver protein secretion"

    Article Title: Feeding-regulated glycogen metabolism drives rhythmic liver protein secretion

    Journal: Nature Metabolism

    doi: 10.1038/s42255-026-01453-8

    a , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in vivo. WT mice under a regular 12 h light–dark cycle and NR feeding were subcutaneously injected with either vehicle (Veh) or CP-91149 at ZT0 for tissue collections at ZT3, ZT6, ZT9, ZT12 and ZT24. b , Average food consumption of WT mice ( n = 4 biological replicates) 24 h after CP-91149 injection. c , Temporal profiles of glycogen in the liver of Veh-injected and CP-91149-injected mice ( n = 20 (5 timepoints × 4 biological replicates)). d , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated mouse liver were determined by lectin blot analysis with concanavalin A (ConA, n = 20 (5 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (right). e , C3 levels in mouse serum as assessed by ELISA ( n = 20 (5 timepoints × 4 biological replicates)). f , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in AML12 mouse hepatocytes. Treatment with Veh or CP-91149 (67 µM) was performed for 3, 6, 12 and 24 h. g , h , Kinetic profiles of UDP-glucose + UDP-galactose levels ( g ), cytidine 5′-monophospho-N-acetyl neuraminic acid (CMP-Neu5Ac) ( h , left) and uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) ( h , right) in AML12 cells upon CP-91149 treatment ( n = 16 (4 timepoints × 4 biological replicates)). i , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated AML12 cells were determined by lectin blot analysis with ConA ( n = 16 (4 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (bottom). j , ALB, FN1 and C3 levels in cell medium determined by ELISA ( n = 22–24 (4 timepoints × 6 biological replicates, with ALB 14 h Veh and 24 h CP-91149 n = 5)). k , Experimental design (created with BioRender.com ). AML12 cells were treated with Veh or CP-91149 for 14 h in the absence or presence of supplemental UDP-glucose (UDPG; 2 mM). l , m , ALB and C3 levels in cell medium ( l ) and lysates ( m ) as determined by ELISA ( n = 6 biological replicates, except for ALB under CP-91149 treatment ( n = 5)). Data are displayed as means; error bars, s.e.m. Boxplots show the median (centre line), interquartile range (box) and minimum to maximum values (whiskers). A detailed description of the statistical analysis is available in Source Data Fig. . See also Extended Data Fig. .
    Figure Legend Snippet: a , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in vivo. WT mice under a regular 12 h light–dark cycle and NR feeding were subcutaneously injected with either vehicle (Veh) or CP-91149 at ZT0 for tissue collections at ZT3, ZT6, ZT9, ZT12 and ZT24. b , Average food consumption of WT mice ( n = 4 biological replicates) 24 h after CP-91149 injection. c , Temporal profiles of glycogen in the liver of Veh-injected and CP-91149-injected mice ( n = 20 (5 timepoints × 4 biological replicates)). d , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated mouse liver were determined by lectin blot analysis with concanavalin A (ConA, n = 20 (5 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (right). e , C3 levels in mouse serum as assessed by ELISA ( n = 20 (5 timepoints × 4 biological replicates)). f , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in AML12 mouse hepatocytes. Treatment with Veh or CP-91149 (67 µM) was performed for 3, 6, 12 and 24 h. g , h , Kinetic profiles of UDP-glucose + UDP-galactose levels ( g ), cytidine 5′-monophospho-N-acetyl neuraminic acid (CMP-Neu5Ac) ( h , left) and uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) ( h , right) in AML12 cells upon CP-91149 treatment ( n = 16 (4 timepoints × 4 biological replicates)). i , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated AML12 cells were determined by lectin blot analysis with ConA ( n = 16 (4 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (bottom). j , ALB, FN1 and C3 levels in cell medium determined by ELISA ( n = 22–24 (4 timepoints × 6 biological replicates, with ALB 14 h Veh and 24 h CP-91149 n = 5)). k , Experimental design (created with BioRender.com ). AML12 cells were treated with Veh or CP-91149 for 14 h in the absence or presence of supplemental UDP-glucose (UDPG; 2 mM). l , m , ALB and C3 levels in cell medium ( l ) and lysates ( m ) as determined by ELISA ( n = 6 biological replicates, except for ALB under CP-91149 treatment ( n = 5)). Data are displayed as means; error bars, s.e.m. Boxplots show the median (centre line), interquartile range (box) and minimum to maximum values (whiskers). A detailed description of the statistical analysis is available in Source Data Fig. . See also Extended Data Fig. .

    Techniques Used: Inhibition, In Vivo, Injection, Glycoproteomics, Staining, Control, Enzyme-linked Immunosorbent Assay

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    Staining:

    Article Title: Inhibitor of ppGalNAc-T3-mediated O-glycosylation blocks cancer cell invasiveness and lowers FGF23 levels
    Article Snippet: .. HeLa cells (treated with T3Inh-1 for 24 hr at the indicated concentrations) were washed twice in PBS containing 0.5% FBS then stained for 30 min with fluorescent lectins ConA (CatFL-1001), WGA (Cat#FL-1021), SNA (Cat#FL-1301) or VVA (Cat#FL-1231) (Vector Laboratories, Burlingame, CA) at 1:100 dilutions (except WGA used at 1:1000) in the wash buffer. .. After staining, the cells were washed twice, lysed with 0.2% Triton X-100 (Fisher Scientific, Cat#BP151-100) for 15 min at 4 ̊C, centrifuged at 14,000 x g for 15 min at 4 ̊C, and the supernatants were read in a Greiner Bio-One 96- well glass-bottom plate using a Tecan Infinite M1000 with excitation at 495 nm and emission from 510–550 nm.

    Article Title: Cell-based screening and compounds that modulate GalNAc-transferases
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    Article Title: Distinctive role of fluG in the adaptation of Beauveria bassiana to insect-pathogenic lifecycle and environmental stresses.
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    Article Title: Inhibitor of ppGalNAc-T3-mediated O-glycosylation blocks cancer cell invasiveness and lowers FGF23 levels
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    Article Title: Cell-based screening and compounds that modulate GaINAc-transferases
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    Whole Genome Amplification:

    Article Title: Inhibitor of ppGalNAc-T3-mediated O-glycosylation blocks cancer cell invasiveness and lowers FGF23 levels
    Article Snippet: .. HeLa cells (treated with T3Inh-1 for 24 hr at the indicated concentrations) were washed twice in PBS containing 0.5% FBS then stained for 30 min with fluorescent lectins ConA (CatFL-1001), WGA (Cat#FL-1021), SNA (Cat#FL-1301) or VVA (Cat#FL-1231) (Vector Laboratories, Burlingame, CA) at 1:100 dilutions (except WGA used at 1:1000) in the wash buffer. .. After staining, the cells were washed twice, lysed with 0.2% Triton X-100 (Fisher Scientific, Cat#BP151-100) for 15 min at 4 ̊C, centrifuged at 14,000 x g for 15 min at 4 ̊C, and the supernatants were read in a Greiner Bio-One 96- well glass-bottom plate using a Tecan Infinite M1000 with excitation at 495 nm and emission from 510–550 nm.

    Article Title: Cell-based screening and compounds that modulate GalNAc-transferases
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    Article Title: Distinctive role of fluG in the adaptation of Beauveria bassiana to insect-pathogenic lifecycle and environmental stresses.
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    Article Title: Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana.
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    Article Title: A Small Cysteine-Free Protein Acts as a Novel Regulator of Fungal Insect-Pathogenic Lifecycle and Genomic Expression
    Article Snippet: .. Next, conidia were labeled with the Alexa Fluor 488-labeled lectins ConA, WGA, PNA, and GNL (Vector Laboratories, Burlingame, CA, USA) according to the manufacturer’s guide. ..

    Article Title: Inhibitor of ppGalNAc-T3-mediated O-glycosylation blocks cancer cell invasiveness and lowers FGF23 levels
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    Article Title: Cell-based screening and compounds that modulate GaINAc-transferases
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    Binding Assay:

    Article Title: Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana.
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    Labeling:

    Article Title: Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana.
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    Article Title: A Small Cysteine-Free Protein Acts as a Novel Regulator of Fungal Insect-Pathogenic Lifecycle and Genomic Expression
    Article Snippet: .. Next, conidia were labeled with the Alexa Fluor 488-labeled lectins ConA, WGA, PNA, and GNL (Vector Laboratories, Burlingame, CA, USA) according to the manufacturer’s guide. ..



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    Vector Laboratories agarose bound lectin cona
    EVs glycoprotein enrichment and proteomic analysis. (A) Workflow for proteome identification of EV total proteins and glycoproteins. (B) Principal component analysis of 95C and 95D EVs total proteins (T‐p) and glycoproteins (G‐p). (C) Venn diagram of EVs total proteins and glycoproteins. (D) Pearson correlation coefficient matrix plot of multiple groups. The correlation coefficient was represented by a color scheme from white (negative correlation) to blue (positive correlation). (E) The volcano plot represents the quantitative analysis of N‐glycoproteins in 95D EVs versus 95C EVs. Orange represents proteins that were at least 2‐fold upregulated ( p < 0.05); blue represents proteins that were at least 2‐fold downregulated ( p < 0.05). (F) Distribution plot of the number of N‐glycosylation sites of 95C and 95D EVs. (G‐I) Comparison of the ranking, relative quantity, and unique peptides of three typical glycoproteins before and after <t>ConA</t> enrichment. GO analysis (J) and KEGG pathway enrichment (K) of differentially expressed glycoproteins in EVs. BP, biological process; CC, cellular component; MF, molecular function.
    Agarose Bound Lectin Cona, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1245 biotinylated cona vector laboratories  (Vector Laboratories)
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    Vector Laboratories 1245 biotinylated cona vector laboratories
    EVs glycoprotein enrichment and proteomic analysis. (A) Workflow for proteome identification of EV total proteins and glycoproteins. (B) Principal component analysis of 95C and 95D EVs total proteins (T‐p) and glycoproteins (G‐p). (C) Venn diagram of EVs total proteins and glycoproteins. (D) Pearson correlation coefficient matrix plot of multiple groups. The correlation coefficient was represented by a color scheme from white (negative correlation) to blue (positive correlation). (E) The volcano plot represents the quantitative analysis of N‐glycoproteins in 95D EVs versus 95C EVs. Orange represents proteins that were at least 2‐fold upregulated ( p < 0.05); blue represents proteins that were at least 2‐fold downregulated ( p < 0.05). (F) Distribution plot of the number of N‐glycosylation sites of 95C and 95D EVs. (G‐I) Comparison of the ranking, relative quantity, and unique peptides of three typical glycoproteins before and after <t>ConA</t> enrichment. GO analysis (J) and KEGG pathway enrichment (K) of differentially expressed glycoproteins in EVs. BP, biological process; CC, cellular component; MF, molecular function.
    1245 Biotinylated Cona Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lectins+cona/Biotinylated+Galanthus+Nivalis+Lectin+(GNL)/pm40608513-271-93-96
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    concanavalin a (cona) lectin  (Seikagaku corporation)
    90
    Seikagaku corporation concanavalin a (cona) lectin
    EVs glycoprotein enrichment and proteomic analysis. (A) Workflow for proteome identification of EV total proteins and glycoproteins. (B) Principal component analysis of 95C and 95D EVs total proteins (T‐p) and glycoproteins (G‐p). (C) Venn diagram of EVs total proteins and glycoproteins. (D) Pearson correlation coefficient matrix plot of multiple groups. The correlation coefficient was represented by a color scheme from white (negative correlation) to blue (positive correlation). (E) The volcano plot represents the quantitative analysis of N‐glycoproteins in 95D EVs versus 95C EVs. Orange represents proteins that were at least 2‐fold upregulated ( p < 0.05); blue represents proteins that were at least 2‐fold downregulated ( p < 0.05). (F) Distribution plot of the number of N‐glycosylation sites of 95C and 95D EVs. (G‐I) Comparison of the ranking, relative quantity, and unique peptides of three typical glycoproteins before and after <t>ConA</t> enrichment. GO analysis (J) and KEGG pathway enrichment (K) of differentially expressed glycoproteins in EVs. BP, biological process; CC, cellular component; MF, molecular function.
    Concanavalin A (Cona) Lectin, supplied by Seikagaku corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lectins+cona/concanavalin+a+cona/pm40409546-191-9-16
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    biotinylated plant lectin cona  (Vector Laboratories)
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    Vector Laboratories biotinylated plant lectin cona
    EVs glycoprotein enrichment and proteomic analysis. (A) Workflow for proteome identification of EV total proteins and glycoproteins. (B) Principal component analysis of 95C and 95D EVs total proteins (T‐p) and glycoproteins (G‐p). (C) Venn diagram of EVs total proteins and glycoproteins. (D) Pearson correlation coefficient matrix plot of multiple groups. The correlation coefficient was represented by a color scheme from white (negative correlation) to blue (positive correlation). (E) The volcano plot represents the quantitative analysis of N‐glycoproteins in 95D EVs versus 95C EVs. Orange represents proteins that were at least 2‐fold upregulated ( p < 0.05); blue represents proteins that were at least 2‐fold downregulated ( p < 0.05). (F) Distribution plot of the number of N‐glycosylation sites of 95C and 95D EVs. (G‐I) Comparison of the ranking, relative quantity, and unique peptides of three typical glycoproteins before and after <t>ConA</t> enrichment. GO analysis (J) and KEGG pathway enrichment (K) of differentially expressed glycoproteins in EVs. BP, biological process; CC, cellular component; MF, molecular function.
    Biotinylated Plant Lectin Cona, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated lectins concanavalin cona  (Vector Laboratories)
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    Vector Laboratories biotinylated lectins concanavalin cona
    Lectin detection profiles. A total of 12 plasmon shift values, measured after 15 min of immobilization, were recorded for each lectin, with the average and standard deviation values represented by each band in the graph. Each lectin is used to create the LPS profiles for each bacterial species injected at 100 μg/mL, as well as a PBS buffer blank reference. The <t>lectins</t> chosen are <t>Concanavalin</t> <t>(ConA),</t> Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA). These lectins will be referred by their abbreviation for the next figures to avoid unnecessary length in the legend.
    Biotinylated Lectins Concanavalin Cona, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lectins+cona/Biotinylated+Concanavalin+A+(Con+A)/pmc12019741-37-0-33
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    beads coupled to the lectin concanavalin a (cona, canavalia ensiformis , cat. no. 234568)  (Millipore)
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    Millipore beads coupled to the lectin concanavalin a (cona, canavalia ensiformis , cat. no. 234568)
    Lectin detection profiles. A total of 12 plasmon shift values, measured after 15 min of immobilization, were recorded for each lectin, with the average and standard deviation values represented by each band in the graph. Each lectin is used to create the LPS profiles for each bacterial species injected at 100 μg/mL, as well as a PBS buffer blank reference. The <t>lectins</t> chosen are <t>Concanavalin</t> <t>(ConA),</t> Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA). These lectins will be referred by their abbreviation for the next figures to avoid unnecessary length in the legend.
    Beads Coupled To The Lectin Concanavalin A (Cona, Canavalia Ensiformis , Cat. No. 234568), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lectins+cona/concanavalin++con/pmc11817834-104-11-21
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    Image Search Results


    a , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in vivo. WT mice under a regular 12 h light–dark cycle and NR feeding were subcutaneously injected with either vehicle (Veh) or CP-91149 at ZT0 for tissue collections at ZT3, ZT6, ZT9, ZT12 and ZT24. b , Average food consumption of WT mice ( n = 4 biological replicates) 24 h after CP-91149 injection. c , Temporal profiles of glycogen in the liver of Veh-injected and CP-91149-injected mice ( n = 20 (5 timepoints × 4 biological replicates)). d , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated mouse liver were determined by lectin blot analysis with concanavalin A (ConA, n = 20 (5 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (right). e , C3 levels in mouse serum as assessed by ELISA ( n = 20 (5 timepoints × 4 biological replicates)). f , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in AML12 mouse hepatocytes. Treatment with Veh or CP-91149 (67 µM) was performed for 3, 6, 12 and 24 h. g , h , Kinetic profiles of UDP-glucose + UDP-galactose levels ( g ), cytidine 5′-monophospho-N-acetyl neuraminic acid (CMP-Neu5Ac) ( h , left) and uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) ( h , right) in AML12 cells upon CP-91149 treatment ( n = 16 (4 timepoints × 4 biological replicates)). i , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated AML12 cells were determined by lectin blot analysis with ConA ( n = 16 (4 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (bottom). j , ALB, FN1 and C3 levels in cell medium determined by ELISA ( n = 22–24 (4 timepoints × 6 biological replicates, with ALB 14 h Veh and 24 h CP-91149 n = 5)). k , Experimental design (created with BioRender.com ). AML12 cells were treated with Veh or CP-91149 for 14 h in the absence or presence of supplemental UDP-glucose (UDPG; 2 mM). l , m , ALB and C3 levels in cell medium ( l ) and lysates ( m ) as determined by ELISA ( n = 6 biological replicates, except for ALB under CP-91149 treatment ( n = 5)). Data are displayed as means; error bars, s.e.m. Boxplots show the median (centre line), interquartile range (box) and minimum to maximum values (whiskers). A detailed description of the statistical analysis is available in Source Data Fig. . See also Extended Data Fig. .

    Journal: Nature Metabolism

    Article Title: Feeding-regulated glycogen metabolism drives rhythmic liver protein secretion

    doi: 10.1038/s42255-026-01453-8

    Figure Lengend Snippet: a , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in vivo. WT mice under a regular 12 h light–dark cycle and NR feeding were subcutaneously injected with either vehicle (Veh) or CP-91149 at ZT0 for tissue collections at ZT3, ZT6, ZT9, ZT12 and ZT24. b , Average food consumption of WT mice ( n = 4 biological replicates) 24 h after CP-91149 injection. c , Temporal profiles of glycogen in the liver of Veh-injected and CP-91149-injected mice ( n = 20 (5 timepoints × 4 biological replicates)). d , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated mouse liver were determined by lectin blot analysis with concanavalin A (ConA, n = 20 (5 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (right). e , C3 levels in mouse serum as assessed by ELISA ( n = 20 (5 timepoints × 4 biological replicates)). f , Experimental design (created with BioRender.com ). Inhibition of PYGL-mediated glycogenolysis by CP-91149 in AML12 mouse hepatocytes. Treatment with Veh or CP-91149 (67 µM) was performed for 3, 6, 12 and 24 h. g , h , Kinetic profiles of UDP-glucose + UDP-galactose levels ( g ), cytidine 5′-monophospho-N-acetyl neuraminic acid (CMP-Neu5Ac) ( h , left) and uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) ( h , right) in AML12 cells upon CP-91149 treatment ( n = 16 (4 timepoints × 4 biological replicates)). i , Glycosylation levels of proteins in CP-91149-treated and vehicle-treated AML12 cells were determined by lectin blot analysis with ConA ( n = 16 (4 timepoints × 4 biological replicates)). Amido black staining of the membranes was used as a loading control and serves as a reference for normalization of the quantified values (bottom). j , ALB, FN1 and C3 levels in cell medium determined by ELISA ( n = 22–24 (4 timepoints × 6 biological replicates, with ALB 14 h Veh and 24 h CP-91149 n = 5)). k , Experimental design (created with BioRender.com ). AML12 cells were treated with Veh or CP-91149 for 14 h in the absence or presence of supplemental UDP-glucose (UDPG; 2 mM). l , m , ALB and C3 levels in cell medium ( l ) and lysates ( m ) as determined by ELISA ( n = 6 biological replicates, except for ALB under CP-91149 treatment ( n = 5)). Data are displayed as means; error bars, s.e.m. Boxplots show the median (centre line), interquartile range (box) and minimum to maximum values (whiskers). A detailed description of the statistical analysis is available in Source Data Fig. . See also Extended Data Fig. .

    Article Snippet: Primary antibodies were used at the following dilutions: 1:1,000 for ATF4 (Cell Signaling Technologies, 11815), ARFGAP1 (Cell Signaling Technologies, 14608), Phospho-RPS6 (Cell Signaling Technologies, 2211), Total-RPS6 (Cell Signaling Technologies, 2217), GABARAPL1 (Genetex, GTX132664) and ConA Lectin (Vector Laboratories, B-1005) and 1:2,000 for STX4 (ProteinTech, 14988-1-AP).

    Techniques: Inhibition, In Vivo, Injection, Glycoproteomics, Staining, Control, Enzyme-linked Immunosorbent Assay

    EVs glycoprotein enrichment and proteomic analysis. (A) Workflow for proteome identification of EV total proteins and glycoproteins. (B) Principal component analysis of 95C and 95D EVs total proteins (T‐p) and glycoproteins (G‐p). (C) Venn diagram of EVs total proteins and glycoproteins. (D) Pearson correlation coefficient matrix plot of multiple groups. The correlation coefficient was represented by a color scheme from white (negative correlation) to blue (positive correlation). (E) The volcano plot represents the quantitative analysis of N‐glycoproteins in 95D EVs versus 95C EVs. Orange represents proteins that were at least 2‐fold upregulated ( p < 0.05); blue represents proteins that were at least 2‐fold downregulated ( p < 0.05). (F) Distribution plot of the number of N‐glycosylation sites of 95C and 95D EVs. (G‐I) Comparison of the ranking, relative quantity, and unique peptides of three typical glycoproteins before and after ConA enrichment. GO analysis (J) and KEGG pathway enrichment (K) of differentially expressed glycoproteins in EVs. BP, biological process; CC, cellular component; MF, molecular function.

    Journal: Journal of Extracellular Vesicles

    Article Title: Proteomic Analysis of Extracellular Vesicles Identifies CDCP1 as Critical Metastasis‐Related Glycoprotein in Lung Cancer

    doi: 10.1002/jev2.70128

    Figure Lengend Snippet: EVs glycoprotein enrichment and proteomic analysis. (A) Workflow for proteome identification of EV total proteins and glycoproteins. (B) Principal component analysis of 95C and 95D EVs total proteins (T‐p) and glycoproteins (G‐p). (C) Venn diagram of EVs total proteins and glycoproteins. (D) Pearson correlation coefficient matrix plot of multiple groups. The correlation coefficient was represented by a color scheme from white (negative correlation) to blue (positive correlation). (E) The volcano plot represents the quantitative analysis of N‐glycoproteins in 95D EVs versus 95C EVs. Orange represents proteins that were at least 2‐fold upregulated ( p < 0.05); blue represents proteins that were at least 2‐fold downregulated ( p < 0.05). (F) Distribution plot of the number of N‐glycosylation sites of 95C and 95D EVs. (G‐I) Comparison of the ranking, relative quantity, and unique peptides of three typical glycoproteins before and after ConA enrichment. GO analysis (J) and KEGG pathway enrichment (K) of differentially expressed glycoproteins in EVs. BP, biological process; CC, cellular component; MF, molecular function.

    Article Snippet: Briefly, for 1 mg EVs protein, 1 mL of agarose bound lectin ConA (Vector Laboratories, AL‐1003) was packed into a 2 mL disposable screw end‐cap spin column with filter (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Glycoproteomics, Comparison

    Lectin detection profiles. A total of 12 plasmon shift values, measured after 15 min of immobilization, were recorded for each lectin, with the average and standard deviation values represented by each band in the graph. Each lectin is used to create the LPS profiles for each bacterial species injected at 100 μg/mL, as well as a PBS buffer blank reference. The lectins chosen are Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA). These lectins will be referred by their abbreviation for the next figures to avoid unnecessary length in the legend.

    Journal: ACS Omega

    Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance

    doi: 10.1021/acsomega.5c00867

    Figure Lengend Snippet: Lectin detection profiles. A total of 12 plasmon shift values, measured after 15 min of immobilization, were recorded for each lectin, with the average and standard deviation values represented by each band in the graph. Each lectin is used to create the LPS profiles for each bacterial species injected at 100 μg/mL, as well as a PBS buffer blank reference. The lectins chosen are Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA). These lectins will be referred by their abbreviation for the next figures to avoid unnecessary length in the legend.

    Article Snippet: Biotinylated lectins Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA) were purchased from Vector Laboratories (Newark, US).

    Techniques: Standard Deviation, Injection

    Feature importance coefficient. Each bar illustrates the mean importance coefficients for the 7 features associated with 7 lectins, calculated from 10 randomly selected train/test splits of the data set using a Random Forest model with 100 trees.

    Journal: ACS Omega

    Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance

    doi: 10.1021/acsomega.5c00867

    Figure Lengend Snippet: Feature importance coefficient. Each bar illustrates the mean importance coefficients for the 7 features associated with 7 lectins, calculated from 10 randomly selected train/test splits of the data set using a Random Forest model with 100 trees.

    Article Snippet: Biotinylated lectins Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA) were purchased from Vector Laboratories (Newark, US).

    Techniques:

    Feature reduction for RF and SVM machine learning models. The best-performing lectin set from the combination of increasing number of lectins (1 to 7, from left to right) is used in a 10 repeated 3-fold cross-validation to determine the accuracy for each model. Significant differences are assessed with a t -test ( p > 0.05). An asterisk indicates a distribution with a statistically significant difference in performance compared to a distribution without it.

    Journal: ACS Omega

    Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance

    doi: 10.1021/acsomega.5c00867

    Figure Lengend Snippet: Feature reduction for RF and SVM machine learning models. The best-performing lectin set from the combination of increasing number of lectins (1 to 7, from left to right) is used in a 10 repeated 3-fold cross-validation to determine the accuracy for each model. Significant differences are assessed with a t -test ( p > 0.05). An asterisk indicates a distribution with a statistically significant difference in performance compared to a distribution without it.

    Article Snippet: Biotinylated lectins Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA) were purchased from Vector Laboratories (Newark, US).

    Techniques:

    Classification Report for Different Profile Lengths with the SVM Model

    Journal: ACS Omega

    Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance

    doi: 10.1021/acsomega.5c00867

    Figure Lengend Snippet: Classification Report for Different Profile Lengths with the SVM Model

    Article Snippet: Biotinylated lectins Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA) were purchased from Vector Laboratories (Newark, US).

    Techniques:

    Classification Report for Different Profile Lengths with the RF Model

    Journal: ACS Omega

    Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance

    doi: 10.1021/acsomega.5c00867

    Figure Lengend Snippet: Classification Report for Different Profile Lengths with the RF Model

    Article Snippet: Biotinylated lectins Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA) were purchased from Vector Laboratories (Newark, US).

    Techniques: