rabbit polyclonal anti lc3b (Novus Biologicals)
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Rabbit Polyclonal Anti Lc3b, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lc3b/LC3B+Antibody+-+BSA+Free/pmc13011206-161-37-42
Average 96 stars, based on 1654 article reviews
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Blocking Assay:Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice. Article Snippet: .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti- SQSTM1/ p62 (1:1000, Ab56416, Abcam), anti- PGC1 alpha (1:1000, ab191838, Abcam), Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice Article Snippet: Nitrocellular membranes were stained with Ponceau S (59803, Cell Signaling), imaged, and cut based on molecular weight. .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti‐SQSTM1/p62 (1:1000, Ab56416, Abcam), anti‐PGC1 alpha (1:1000, ab191838, Abcam), Incubation:Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice. Article Snippet: .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti- SQSTM1/ p62 (1:1000, Ab56416, Abcam), anti- PGC1 alpha (1:1000, ab191838, Abcam), Article Title: The CHI3L1 protein plays role as a protecting factor against autophagy in glioblastoma cells Article Snippet: .. Primary antibody incubation was conducted overnight at 4 °C at the following concentrations: for CHI3L1 (rabbit polyclonal antibody, ab180569, Abcam), for Article Title: The CHI3L1 protein plays role as a protecting factor against autophagy in glioblastoma cells Article Snippet: .. Primary antibody incubation was conducted overnight at 4 °C at the following concentrations: for CHI3L1 (rabbit polyclonal antibody, ab180569, Abcam), for Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice Article Snippet: Nitrocellular membranes were stained with Ponceau S (59803, Cell Signaling), imaged, and cut based on molecular weight. .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti‐SQSTM1/p62 (1:1000, Ab56416, Abcam), anti‐PGC1 alpha (1:1000, ab191838, Abcam), Western Blot:Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice. Article Snippet: .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti- SQSTM1/ p62 (1:1000, Ab56416, Abcam), anti- PGC1 alpha (1:1000, ab191838, Abcam), Article Title: Selective autophagy of whole micronuclei suppresses chromosomal instability Article Snippet: .. ATG16L1 (Cell Signaling, 8089S, WB 1:1000), ATG7 (Cell Signaling, 8558S, WB 1:1000), LC3B (Novus Biologicals, NB100-2220, WB 1:1000), GABARAP (Cell Signaling, 13733S, WB 1:1000); yH2AX (Merck, 05-636, IF 1:1000), Lamin B1 (abcam, ab16048, IF 1:750) .. RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel, 740955) following the manufacturer’s protocol. cDNA was produced using M-MLV reverse transcriptase (Promega, M5313). qPCR was performed on a ViiA 7 machine (ThermoFisher) using PowerUp SYBR Green Master Mix (ThermoFisher, A25742) following the manufacturer’s protocol. Article Title: Effect of reticulocalbin 3 on monocyte-to-macrophage differentiation in sepsis through modulating autophagy Article Snippet: .. The samples were subjected to Western blotting with specific antibodies against RCN3 (Sigma, #HPA050402), Article Title: Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male Mice Article Snippet: Nitrocellular membranes were stained with Ponceau S (59803, Cell Signaling), imaged, and cut based on molecular weight. .. After blocking in 5% nonfat dry milk, membranes were incubated overnight at 4°C with the following primary antibodies: anti‐SQSTM1/p62 (1:1000, Ab56416, Abcam), anti‐PGC1 alpha (1:1000, ab191838, Abcam), Article Title: Targeting VPS4 elicits STING-driven anti-tumor immunity to suppress rhabdomyosarcoma growth. Article Snippet: .. The following antibodies were used for immunoblotting: GFP (Cell Signaling Technology [Danvers, MA, USA], #2956S, 1:1000), IRF3 (Cell Signaling Technology #4302S, 1:1000), phospho-TBK1 (Cell Signaling Technology #5483S, 1:1000), TBK1 (Cell Signaling Technology #71543SF, 1:1000), phospho-STING (Cell Signaling Technology #72971S, 1:1000), STING (Cell Signaling Technology #13647S, 1:1000), Cleaved caspase 3 (Cell Signaling Technology #9661S, 1:1000), β-actin (Sigma-Aldrich [St. Louis, MI, USA], #A5441, 1:10,000), VPS4A (Sigma-Aldrich #SAB4200215, 1:1000), VPS4B (Santa Cruz Biotechnology [Dallas, TX, USA], #sc-377162, 1:200), cGAS (Cell Signaling Technology #31659S, 1:1000), p62/SQSTM1 (PROGEN Biotechnik [Heidelberg, Germany], #GP62-C, 1:1000), ATF-3 (Cell Signaling Technology #18665S, 1:1000), and other:Article Title: Autophagy is essential for anti- Wolbachia drug efficacy in Brugia malayi and insect cells Article Snippet: LC3B , Rabbit , 1:1000 , 5% non-fat milk in |

