lamp3 (Novus Biologicals)
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lamp3
Lamp3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamp3/DC-LAMP+Antibody+(1010E1%2E01)/bio_rxiv__64898__2026__03__20__713166-315-23-24
Average 94 stars, based on 33 article reviews
Lamp3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamp3/DC-LAMP+Antibody+(1010E1%2E01)/bio_rxiv__64898__2026__03__20__713166-315-23-24
Average 94 stars, based on 33 article reviews
lamp3 - by Bioz Stars,
2026-09
94/100 stars
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Staining:Article Title: A postnatal human lung developmental atlas reveals windows of genetic vulnerability to chronic lung disease Article Snippet: .. The following antibodies were used for IF staining: human HLA-II (rabbit, Thermo Fisher cat. RBM1-2967-P0, 1:100); VE-Cadherin (mouse, Thermo Fisher cat. 5012896, 1:100), LAMP3( Article Title: Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate Article Snippet: Article Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate Immunohistochemistry:Article Title: Airway epithelial cell identity and plasticity are constrained by Sox2 during lung homeostasis, tissue regeneration, and in human disease. Article Snippet: .. For immunohistochemistry, the following antibodies were used: GFP (chicken, Aves, GFP-1020, 1:200), Nkx2-1 (rabbit, Abcam, ab76013, 1:100), Sox2 (goat, R&D, AF2018, 1:50), Sox9 (rabbit, Abcam, ab185966, 1:100), Scgb1a1 (mouse, Santa Cruz, sc-365992, 1:100), SCGB1A1 (rat, R&D, MAB4218, 1:40), Scgb3a2 (mouse, Novus, H00117156-M01, 1:100), β-Tubulin IV (mouse, Biogenex, MU178-UC, 1:20), p63 (rabbit, Santa Cruz, sc-8343, 1:100), β-catenin (mouse, BD Biosciences, 610154, 1:100), Sftpc (rabbit, Millipore, AB3786, 1:200), SFTPC (rabbit, Abcam, ab90716, 1:100), Article Title: Airway epithelial cell identity and plasticity are constrained by Sox2 during lung homeostasis, tissue regeneration, and in human disease Article Snippet: .. For immunohistochemistry, the following antibodies were used: GFP (chicken, Aves, GFP-1020, 1:200), Nkx2-1 (rabbit, Abcam, ab76013, 1:100), Sox2 (goat, R&D, AF2018, 1:50), Sox9 (rabbit, Abcam, ab185966, 1:100), Scgb1a1 (mouse, Santa Cruz, sc-365992, 1:100), SCGB1A1 (rat, R&D, MAB4218, 1:40), Scgb3a2 (mouse, Novus, H00117156-M01, 1:100), β-Tubulin IV (mouse, Biogenex, MU178-UC, 1:20), p63 (rabbit, Santa Cruz, sc-8343, 1:100), β-catenin (mouse, BD Biosciences, 610154, 1:100), Sftpc (rabbit, Millipore, AB3786, 1:200), SFTPC (rabbit, Abcam, ab90716, 1:100), Article Title: Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate Article Snippet: Article Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate Blocking Assay:Article Title: Mouse models of lung-specific SARS-CoV-2 infection with moderate pathological traits. Article Snippet: .. To perform the antigen retrieval step, pH 6.0 citrate buffer (Dako S1699; Agilent Technologies, Santa Clara, CA, USA) was used under high temperature in a high-pressure cooker and then cooled on ice for 1 h. The slides were treated with 3% H2O2 in PBS for 30 min to block endogenous peroxidase activity, followed by treatment with M.O.M reagent (Vector Laboratories, Burlingame, CA, USA) for 1 h, and incubated with the following mouse primary antibodies at 4 °C overnight: SARS-CoV-2 N protein (NB100-56576, Novus and 40143-MM08, Sino Biological), SCGB1A1 (ABS1673, Sigma), Activity Assay:Article Title: Mouse models of lung-specific SARS-CoV-2 infection with moderate pathological traits. Article Snippet: .. To perform the antigen retrieval step, pH 6.0 citrate buffer (Dako S1699; Agilent Technologies, Santa Clara, CA, USA) was used under high temperature in a high-pressure cooker and then cooled on ice for 1 h. The slides were treated with 3% H2O2 in PBS for 30 min to block endogenous peroxidase activity, followed by treatment with M.O.M reagent (Vector Laboratories, Burlingame, CA, USA) for 1 h, and incubated with the following mouse primary antibodies at 4 °C overnight: SARS-CoV-2 N protein (NB100-56576, Novus and 40143-MM08, Sino Biological), SCGB1A1 (ABS1673, Sigma), Incubation:Article Title: Mouse models of lung-specific SARS-CoV-2 infection with moderate pathological traits. Article Snippet: .. To perform the antigen retrieval step, pH 6.0 citrate buffer (Dako S1699; Agilent Technologies, Santa Clara, CA, USA) was used under high temperature in a high-pressure cooker and then cooled on ice for 1 h. The slides were treated with 3% H2O2 in PBS for 30 min to block endogenous peroxidase activity, followed by treatment with M.O.M reagent (Vector Laboratories, Burlingame, CA, USA) for 1 h, and incubated with the following mouse primary antibodies at 4 °C overnight: SARS-CoV-2 N protein (NB100-56576, Novus and 40143-MM08, Sino Biological), SCGB1A1 (ABS1673, Sigma), |
![(A) Representative images and quantification of CCR7 + DCs (panCK − HLA-DR + <t>LAMP3</t> + , yellow) near BVs (CD31 + PDPN − , magenta), or LVs (CD31 + PDPN + , cyan) in human tumors (HNSCC, NSCLC, and EC). Scale bar represents 20 μm. Whole-tumor sections were analyzed for EC and NSCLC. Numbers of fields of view (FOVs) analyzed per HNSCC sample are as follows: HNSCC1–04 n = 7; HNSCC1–06 n = 16; HNSCC1–07 n = 11; HNSCC2–01 n = 126; HNSCC2–06 n = 455; HNSCC2–09 n = 180; HNSCC2–11 n = 122; HNSCC2–12 n = 79; HNSCC2–15 n = 205; HNSCC2–26 n = 293; HNSCC2–35 n = 175. One bar = one patient . (B) Representative images and quantification of CCR7 + DCs (FSCN1 + ; yellow) located near BVs (CD31 + LYVE-1 − ; magenta) or LVs (CD31 + LYVE-1 + ; cyan) in mouse tumors (MC38, B16F10, and D4M3.A-OVA). Scale bar represents 10 μm. Whole-tumor sections were analyzed. One bar = one mouse. (C) Frequencies of BV-, LV- and non-vessel-associated CCR7 + DCs in mouse MC38 tumors 3 days post anti-CD40 or anti-PD-1 treatment. Whole-tumor sections were analyzed. One bar = one mouse. (D) (Left) Representative images of CCR7 + DCs (FSCN1 + ; yellow) located near BVs (CD31 + LYVE-1 − ; magenta) in MC38 tumors inoculated in Ccr7 ko/wt and Ccr7 ko/ko mice, 3 days post anti-PD-1 treatment. (Right) Distribution of the area of CCR7 + DC surfaces in clusters relative to their distance to closest BVs and plotted as percentage of total CCR7 + DC cluster area. CCR7 + DC surfaces from clusters associated with LVs and those not in clusters were excluded from the analysis. Scale bar represents 20 μm. Whole-tumor sections were analyzed. One dot = average value of all clusters in each genotype ( Ccr7 ko/ko n = 5 mice, 56 clusters; Ccr7 wt /ko n = 6 mice, 28 clusters; and Ccr7 wt /wt n = 3 mice, 19 clusters). Two-way ANOVA with multiple comparisons, mean with SEM; **** p < 0.0001 for comparison at 10 and 20 μm from closest BVs. (E) (Left) Representative images of CCR7 + DCs (FSCN1 + ; yellow) located near BVs (CD31 + LYVE-1 − ; magenta) and Ccl19 ( Ccl19 -eYFP + Tomato + ; white) in Ccl19 -ieYFP reporter mice (left image) or CCL21 (white, right image) in MC38 tumors. (Right) Frequencies of perivascular CCR7 + DC clusters associated with Ccl19 -covered BVs or within CCL21 + areas of the tumors among total perivascular CCR7 + DC clusters. Scale bar represents 20 μm. Whole-tumor sections were analyzed. One dot = one mouse. Unpaired t test, mean with SEM; *** p < 0.001. (F) (Left) Representative images of CCR7 + DCs (FSCN1 + ; yellow) located near BVs (CD31 + LYVE-1 − ; magenta) in MC38 tumors inoculated in Ccl19 wt/wt and Ccl19 ko/ko mice, 2 days post anti-PD-1treatment. (Right) Quantification of BV- or LV-associated CCR7 + DC clusters in MC38 tumors from Ccl19 wt/wt and Ccl19 ko/ko mice. Scale bar represents 20 μm. Whole-tumor sections were analyzed. One dot = one mouse, whiskers represent min to max. Unpaired t test; * p < 0.05. (G) Heatmap depicts log 2 -transformed averaged expression of Ccl19 in indicated immune and non-immune populations in the TME of multiple mouse tumor models (breast, , lung [and GSE201247 ], and pancreatic , ). (H) (Left) Synthetic images of CCR7 + DCs (yellow), blood endothelial cells (BECs; magenta), lymphatic endothelial cells (LECs; cyan), and CCL19 + fibroblasts (green) in one representative NSCLC patient analyzed by spatial transcriptomics. (Right) Box plots depict the enrichment scores of CCL19 + fibroblasts within the neighborhood of BV-associated CCR7 + DCs, in four human NSCLC. Data are shown for both permuted (median enrichment scores from 1,000 permutations) and observed datasets. Scale bar represents 20 μm. Whole-tumor sections were analyzed. One dot = one sample. Paired t test, whiskers represent mean to max; * p < 0.05. (I) Heatmap depicts log 2 -transformed averaged expression of CCL19 in indicated immune and non-immune populations in the TME of multiple human cancer types (HNSCC, n = 40, n = 18 patients; CRC, n = 23, n = 64 patients; ESCC, n = 58 patients ; NSCLC, n = 32, n = 7 patients; BRCA, n = 29 patients ; and PRCA, n = 18 patients ). A cross indicates that the cellular population was not detected. See also – .](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2814/pmc12882814/pmc12882814__nihms-2134284-f0002.jpg)
