Review



anti lamin b1  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Proteintech anti lamin b1
    Anti Lamin B1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+b1/Lamin+B1+Antibody/pm42905836-282-19-22
    Average 96 stars, based on 1870 article reviews
    anti lamin b1 - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: African swine fever virus protein R298L opposes the NF-κB signaling pathway and interferon-mediated pathway.
    Article Snippet: African swine fever virus (ASFV) is a large DNA virus that is highly pathogenic to domestic pigs and severely threatens the global pig industry.. ASFV has evolved a range of strategies to evade or suppress the innate antiviral immunity of the host in order to promote its own infection and proliferation.. However, the molecular mechanisms underlying the inhibition of innate antiviral immunity by ASFV-encoded proteins remain unclear.

    Reporter Assay:

    Article Title: African swine fever virus protein R298L opposes the NF-κB signaling pathway and interferon-mediated pathway.
    Article Snippet: African swine fever virus (ASFV) is a large DNA virus that is highly pathogenic to domestic pigs and severely threatens the global pig industry.. ASFV has evolved a range of strategies to evade or suppress the innate antiviral immunity of the host in order to promote its own infection and proliferation.. However, the molecular mechanisms underlying the inhibition of innate antiviral immunity by ASFV-encoded proteins remain unclear.

    Bioprocessing:

    Article Title: African swine fever virus protein R298L opposes the NF-κB signaling pathway and interferon-mediated pathway.
    Article Snippet: African swine fever virus (ASFV) is a large DNA virus that is highly pathogenic to domestic pigs and severely threatens the global pig industry.. ASFV has evolved a range of strategies to evade or suppress the innate antiviral immunity of the host in order to promote its own infection and proliferation.. However, the molecular mechanisms underlying the inhibition of innate antiviral immunity by ASFV-encoded proteins remain unclear.

    Hemagglutination Assay:

    Article Title: African swine fever virus protein R298L opposes the NF-κB signaling pathway and interferon-mediated pathway.
    Article Snippet: African swine fever virus (ASFV) is a large DNA virus that is highly pathogenic to domestic pigs and severely threatens the global pig industry.. ASFV has evolved a range of strategies to evade or suppress the innate antiviral immunity of the host in order to promote its own infection and proliferation.. However, the molecular mechanisms underlying the inhibition of innate antiviral immunity by ASFV-encoded proteins remain unclear.

    Cell-Signaling:

    Article Title: African swine fever virus protein R298L opposes the NF-κB signaling pathway and interferon-mediated pathway.
    Article Snippet: African swine fever virus (ASFV) is a large DNA virus that is highly pathogenic to domestic pigs and severely threatens the global pig industry.. ASFV has evolved a range of strategies to evade or suppress the innate antiviral immunity of the host in order to promote its own infection and proliferation.. However, the molecular mechanisms underlying the inhibition of innate antiviral immunity by ASFV-encoded proteins remain unclear.



    Similar Products

    96
    Proteintech anti lamin b1
    Anti Lamin B1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+b1/Lamin+B1+Antibody/pm42905836-282-19-22
    Average 96 stars, based on 1 article reviews
    anti lamin b1 - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    95
    ABclonal Biotechnology lamin b1
    FLLL32 targets STAT3 to suppress pSTAT3-mediated GPX4/SLC40A1 transcriptional regulation. a Western blot analysis of pSTAT3, total STAT3, and β-Tubulin in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. b Nuclear-cytoplasmic fractionation analysis of pSTAT3 and STAT3 in HCT116 cells treated with DMSO or FLLL32. Lamin <t>B1</t> and β-Tubulin were used as nuclear and cytoplasmic markers. c Predicted STAT3-binding motif within the GPX4 and SLC40A1 promoter regions. d , e ChIP-qPCR analysis of pSTAT3 occupancy at GPX4 and SLC40A1 promoter regions in HCT116 cells treated with DMSO or FLLL32. IgG was used as the negative control. f , g Promoter luciferase assays showing the activity of wild-type or mutant GPX4 and SLC40A1 promoters in cells transfected with control or STAT3 expression plasmids. h , i Dual-luciferase reporter assays of wild-type or STAT3-binding motif-mutant GPX4 (h) and SLC40A1 (i) promoter reporters in STAT3-overexpressing cells after DMSO or FLLL32 treatment. Mut indicates mutation of the predicted STAT3-binding motif. j Molecular docking model showing the predicted interaction between FLLL32 and STAT3 at Glu638 and Met726. k , l Bio-layer interferometry analysis of the binding between FLLL32 and purified STAT3-WT and STAT3-TB2 proteins. m Western blot analysis of HA, pSTAT3, SLC40A1, GPX4 in STAT3-WT- or STAT3-TB2-expressing cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. n Quantification of Liperfluo fluorescence intensity showing lipid peroxidation in HA-STAT3-WT- or HA-STAT3-TB2-expressing cells after DMSO or FLLL32 treatment. o Quantification of relative EdU-positive cells in HCT116 cells expressing HA-STAT3-WT or HA-STAT3-TB2 after DMSO or FLLL32 treatment. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Lamin B1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+b1/Lamin+B1+Rabbit+mAb/pmc13683295-320-31-33
    Average 95 stars, based on 1 article reviews
    lamin b1 - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    96
    Santa Cruz Biotechnology mouse anti lamin b1
    FLLL32 targets STAT3 to suppress pSTAT3-mediated GPX4/SLC40A1 transcriptional regulation. a Western blot analysis of pSTAT3, total STAT3, and β-Tubulin in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. b Nuclear-cytoplasmic fractionation analysis of pSTAT3 and STAT3 in HCT116 cells treated with DMSO or FLLL32. Lamin <t>B1</t> and β-Tubulin were used as nuclear and cytoplasmic markers. c Predicted STAT3-binding motif within the GPX4 and SLC40A1 promoter regions. d , e ChIP-qPCR analysis of pSTAT3 occupancy at GPX4 and SLC40A1 promoter regions in HCT116 cells treated with DMSO or FLLL32. IgG was used as the negative control. f , g Promoter luciferase assays showing the activity of wild-type or mutant GPX4 and SLC40A1 promoters in cells transfected with control or STAT3 expression plasmids. h , i Dual-luciferase reporter assays of wild-type or STAT3-binding motif-mutant GPX4 (h) and SLC40A1 (i) promoter reporters in STAT3-overexpressing cells after DMSO or FLLL32 treatment. Mut indicates mutation of the predicted STAT3-binding motif. j Molecular docking model showing the predicted interaction between FLLL32 and STAT3 at Glu638 and Met726. k , l Bio-layer interferometry analysis of the binding between FLLL32 and purified STAT3-WT and STAT3-TB2 proteins. m Western blot analysis of HA, pSTAT3, SLC40A1, GPX4 in STAT3-WT- or STAT3-TB2-expressing cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. n Quantification of Liperfluo fluorescence intensity showing lipid peroxidation in HA-STAT3-WT- or HA-STAT3-TB2-expressing cells after DMSO or FLLL32 treatment. o Quantification of relative EdU-positive cells in HCT116 cells expressing HA-STAT3-WT or HA-STAT3-TB2 after DMSO or FLLL32 treatment. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Mouse Anti Lamin B1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+b1/Lamin+B1+Antibody/pm42885327-70-8-13
    Average 96 stars, based on 1 article reviews
    mouse anti lamin b1 - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Proteintech lamin b1 polyclonal antibody
    FLLL32 targets STAT3 to suppress pSTAT3-mediated GPX4/SLC40A1 transcriptional regulation. a Western blot analysis of pSTAT3, total STAT3, and β-Tubulin in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. b Nuclear-cytoplasmic fractionation analysis of pSTAT3 and STAT3 in HCT116 cells treated with DMSO or FLLL32. Lamin <t>B1</t> and β-Tubulin were used as nuclear and cytoplasmic markers. c Predicted STAT3-binding motif within the GPX4 and SLC40A1 promoter regions. d , e ChIP-qPCR analysis of pSTAT3 occupancy at GPX4 and SLC40A1 promoter regions in HCT116 cells treated with DMSO or FLLL32. IgG was used as the negative control. f , g Promoter luciferase assays showing the activity of wild-type or mutant GPX4 and SLC40A1 promoters in cells transfected with control or STAT3 expression plasmids. h , i Dual-luciferase reporter assays of wild-type or STAT3-binding motif-mutant GPX4 (h) and SLC40A1 (i) promoter reporters in STAT3-overexpressing cells after DMSO or FLLL32 treatment. Mut indicates mutation of the predicted STAT3-binding motif. j Molecular docking model showing the predicted interaction between FLLL32 and STAT3 at Glu638 and Met726. k , l Bio-layer interferometry analysis of the binding between FLLL32 and purified STAT3-WT and STAT3-TB2 proteins. m Western blot analysis of HA, pSTAT3, SLC40A1, GPX4 in STAT3-WT- or STAT3-TB2-expressing cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. n Quantification of Liperfluo fluorescence intensity showing lipid peroxidation in HA-STAT3-WT- or HA-STAT3-TB2-expressing cells after DMSO or FLLL32 treatment. o Quantification of relative EdU-positive cells in HCT116 cells expressing HA-STAT3-WT or HA-STAT3-TB2 after DMSO or FLLL32 treatment. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Lamin B1 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+b1/Lamin+B1+Antibody/pm42880457-123-0-29
    Average 96 stars, based on 1 article reviews
    lamin b1 polyclonal antibody - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Santa Cruz Biotechnology lamin b
    FLLL32 targets STAT3 to suppress pSTAT3-mediated GPX4/SLC40A1 transcriptional regulation. a Western blot analysis of pSTAT3, total STAT3, and β-Tubulin in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. b Nuclear-cytoplasmic fractionation analysis of pSTAT3 and STAT3 in HCT116 cells treated with DMSO or FLLL32. Lamin <t>B1</t> and β-Tubulin were used as nuclear and cytoplasmic markers. c Predicted STAT3-binding motif within the GPX4 and SLC40A1 promoter regions. d , e ChIP-qPCR analysis of pSTAT3 occupancy at GPX4 and SLC40A1 promoter regions in HCT116 cells treated with DMSO or FLLL32. IgG was used as the negative control. f , g Promoter luciferase assays showing the activity of wild-type or mutant GPX4 and SLC40A1 promoters in cells transfected with control or STAT3 expression plasmids. h , i Dual-luciferase reporter assays of wild-type or STAT3-binding motif-mutant GPX4 (h) and SLC40A1 (i) promoter reporters in STAT3-overexpressing cells after DMSO or FLLL32 treatment. Mut indicates mutation of the predicted STAT3-binding motif. j Molecular docking model showing the predicted interaction between FLLL32 and STAT3 at Glu638 and Met726. k , l Bio-layer interferometry analysis of the binding between FLLL32 and purified STAT3-WT and STAT3-TB2 proteins. m Western blot analysis of HA, pSTAT3, SLC40A1, GPX4 in STAT3-WT- or STAT3-TB2-expressing cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. n Quantification of Liperfluo fluorescence intensity showing lipid peroxidation in HA-STAT3-WT- or HA-STAT3-TB2-expressing cells after DMSO or FLLL32 treatment. o Quantification of relative EdU-positive cells in HCT116 cells expressing HA-STAT3-WT or HA-STAT3-TB2 after DMSO or FLLL32 treatment. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Lamin B, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+b1/Lamin+B1+Antibody/10__1096_slash_fj__202600413rrr-78-0-5
    Average 96 stars, based on 1 article reviews
    lamin b - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Proteintech anti laminb1
    FLLL32 targets STAT3 to suppress pSTAT3-mediated GPX4/SLC40A1 transcriptional regulation. a Western blot analysis of pSTAT3, total STAT3, and β-Tubulin in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. b Nuclear-cytoplasmic fractionation analysis of pSTAT3 and STAT3 in HCT116 cells treated with DMSO or FLLL32. Lamin <t>B1</t> and β-Tubulin were used as nuclear and cytoplasmic markers. c Predicted STAT3-binding motif within the GPX4 and SLC40A1 promoter regions. d , e ChIP-qPCR analysis of pSTAT3 occupancy at GPX4 and SLC40A1 promoter regions in HCT116 cells treated with DMSO or FLLL32. IgG was used as the negative control. f , g Promoter luciferase assays showing the activity of wild-type or mutant GPX4 and SLC40A1 promoters in cells transfected with control or STAT3 expression plasmids. h , i Dual-luciferase reporter assays of wild-type or STAT3-binding motif-mutant GPX4 (h) and SLC40A1 (i) promoter reporters in STAT3-overexpressing cells after DMSO or FLLL32 treatment. Mut indicates mutation of the predicted STAT3-binding motif. j Molecular docking model showing the predicted interaction between FLLL32 and STAT3 at Glu638 and Met726. k , l Bio-layer interferometry analysis of the binding between FLLL32 and purified STAT3-WT and STAT3-TB2 proteins. m Western blot analysis of HA, pSTAT3, SLC40A1, GPX4 in STAT3-WT- or STAT3-TB2-expressing cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. n Quantification of Liperfluo fluorescence intensity showing lipid peroxidation in HA-STAT3-WT- or HA-STAT3-TB2-expressing cells after DMSO or FLLL32 treatment. o Quantification of relative EdU-positive cells in HCT116 cells expressing HA-STAT3-WT or HA-STAT3-TB2 after DMSO or FLLL32 treatment. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Anti Laminb1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+b1/Lamin+B1+Antibody/pm42863694-93-12-14
    Average 96 stars, based on 1 article reviews
    anti laminb1 - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Proteintech anti lamin b1 monoclonal antibody
    FLLL32 targets STAT3 to suppress pSTAT3-mediated GPX4/SLC40A1 transcriptional regulation. a Western blot analysis of pSTAT3, total STAT3, and β-Tubulin in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. b Nuclear-cytoplasmic fractionation analysis of pSTAT3 and STAT3 in HCT116 cells treated with DMSO or FLLL32. Lamin <t>B1</t> and β-Tubulin were used as nuclear and cytoplasmic markers. c Predicted STAT3-binding motif within the GPX4 and SLC40A1 promoter regions. d , e ChIP-qPCR analysis of pSTAT3 occupancy at GPX4 and SLC40A1 promoter regions in HCT116 cells treated with DMSO or FLLL32. IgG was used as the negative control. f , g Promoter luciferase assays showing the activity of wild-type or mutant GPX4 and SLC40A1 promoters in cells transfected with control or STAT3 expression plasmids. h , i Dual-luciferase reporter assays of wild-type or STAT3-binding motif-mutant GPX4 (h) and SLC40A1 (i) promoter reporters in STAT3-overexpressing cells after DMSO or FLLL32 treatment. Mut indicates mutation of the predicted STAT3-binding motif. j Molecular docking model showing the predicted interaction between FLLL32 and STAT3 at Glu638 and Met726. k , l Bio-layer interferometry analysis of the binding between FLLL32 and purified STAT3-WT and STAT3-TB2 proteins. m Western blot analysis of HA, pSTAT3, SLC40A1, GPX4 in STAT3-WT- or STAT3-TB2-expressing cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. n Quantification of Liperfluo fluorescence intensity showing lipid peroxidation in HA-STAT3-WT- or HA-STAT3-TB2-expressing cells after DMSO or FLLL32 treatment. o Quantification of relative EdU-positive cells in HCT116 cells expressing HA-STAT3-WT or HA-STAT3-TB2 after DMSO or FLLL32 treatment. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Anti Lamin B1 Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+b1/Lamin+B1+Antibody/pm42864499-501-201-205
    Average 96 stars, based on 1 article reviews
    anti lamin b1 monoclonal antibody - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Santa Cruz Biotechnology lamin b1
    FLLL32 targets STAT3 to suppress pSTAT3-mediated GPX4/SLC40A1 transcriptional regulation. a Western blot analysis of pSTAT3, total STAT3, and β-Tubulin in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. b Nuclear-cytoplasmic fractionation analysis of pSTAT3 and STAT3 in HCT116 cells treated with DMSO or FLLL32. Lamin <t>B1</t> and β-Tubulin were used as nuclear and cytoplasmic markers. c Predicted STAT3-binding motif within the GPX4 and SLC40A1 promoter regions. d , e ChIP-qPCR analysis of pSTAT3 occupancy at GPX4 and SLC40A1 promoter regions in HCT116 cells treated with DMSO or FLLL32. IgG was used as the negative control. f , g Promoter luciferase assays showing the activity of wild-type or mutant GPX4 and SLC40A1 promoters in cells transfected with control or STAT3 expression plasmids. h , i Dual-luciferase reporter assays of wild-type or STAT3-binding motif-mutant GPX4 (h) and SLC40A1 (i) promoter reporters in STAT3-overexpressing cells after DMSO or FLLL32 treatment. Mut indicates mutation of the predicted STAT3-binding motif. j Molecular docking model showing the predicted interaction between FLLL32 and STAT3 at Glu638 and Met726. k , l Bio-layer interferometry analysis of the binding between FLLL32 and purified STAT3-WT and STAT3-TB2 proteins. m Western blot analysis of HA, pSTAT3, SLC40A1, GPX4 in STAT3-WT- or STAT3-TB2-expressing cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. n Quantification of Liperfluo fluorescence intensity showing lipid peroxidation in HA-STAT3-WT- or HA-STAT3-TB2-expressing cells after DMSO or FLLL32 treatment. o Quantification of relative EdU-positive cells in HCT116 cells expressing HA-STAT3-WT or HA-STAT3-TB2 after DMSO or FLLL32 treatment. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Lamin B1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+b1/Lamin+B1+Antibody/pm42854374-109-23-25
    Average 96 stars, based on 1 article reviews
    lamin b1 - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Proteintech anti lmnb1
    FLLL32 targets STAT3 to suppress pSTAT3-mediated GPX4/SLC40A1 transcriptional regulation. a Western blot analysis of pSTAT3, total STAT3, and β-Tubulin in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. b Nuclear-cytoplasmic fractionation analysis of pSTAT3 and STAT3 in HCT116 cells treated with DMSO or FLLL32. Lamin <t>B1</t> and β-Tubulin were used as nuclear and cytoplasmic markers. c Predicted STAT3-binding motif within the GPX4 and SLC40A1 promoter regions. d , e ChIP-qPCR analysis of pSTAT3 occupancy at GPX4 and SLC40A1 promoter regions in HCT116 cells treated with DMSO or FLLL32. IgG was used as the negative control. f , g Promoter luciferase assays showing the activity of wild-type or mutant GPX4 and SLC40A1 promoters in cells transfected with control or STAT3 expression plasmids. h , i Dual-luciferase reporter assays of wild-type or STAT3-binding motif-mutant GPX4 (h) and SLC40A1 (i) promoter reporters in STAT3-overexpressing cells after DMSO or FLLL32 treatment. Mut indicates mutation of the predicted STAT3-binding motif. j Molecular docking model showing the predicted interaction between FLLL32 and STAT3 at Glu638 and Met726. k , l Bio-layer interferometry analysis of the binding between FLLL32 and purified STAT3-WT and STAT3-TB2 proteins. m Western blot analysis of HA, pSTAT3, SLC40A1, GPX4 in STAT3-WT- or STAT3-TB2-expressing cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. n Quantification of Liperfluo fluorescence intensity showing lipid peroxidation in HA-STAT3-WT- or HA-STAT3-TB2-expressing cells after DMSO or FLLL32 treatment. o Quantification of relative EdU-positive cells in HCT116 cells expressing HA-STAT3-WT or HA-STAT3-TB2 after DMSO or FLLL32 treatment. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Anti Lmnb1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+b1/Lamin+B1+Antibody/pm42785177-36-31-37
    Average 96 stars, based on 1 article reviews
    anti lmnb1 - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Proteintech lamin b1
    FLLL32 targets STAT3 to suppress pSTAT3-mediated GPX4/SLC40A1 transcriptional regulation. a Western blot analysis of pSTAT3, total STAT3, and β-Tubulin in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. b Nuclear-cytoplasmic fractionation analysis of pSTAT3 and STAT3 in HCT116 cells treated with DMSO or FLLL32. Lamin <t>B1</t> and β-Tubulin were used as nuclear and cytoplasmic markers. c Predicted STAT3-binding motif within the GPX4 and SLC40A1 promoter regions. d , e ChIP-qPCR analysis of pSTAT3 occupancy at GPX4 and SLC40A1 promoter regions in HCT116 cells treated with DMSO or FLLL32. IgG was used as the negative control. f , g Promoter luciferase assays showing the activity of wild-type or mutant GPX4 and SLC40A1 promoters in cells transfected with control or STAT3 expression plasmids. h , i Dual-luciferase reporter assays of wild-type or STAT3-binding motif-mutant GPX4 (h) and SLC40A1 (i) promoter reporters in STAT3-overexpressing cells after DMSO or FLLL32 treatment. Mut indicates mutation of the predicted STAT3-binding motif. j Molecular docking model showing the predicted interaction between FLLL32 and STAT3 at Glu638 and Met726. k , l Bio-layer interferometry analysis of the binding between FLLL32 and purified STAT3-WT and STAT3-TB2 proteins. m Western blot analysis of HA, pSTAT3, SLC40A1, GPX4 in STAT3-WT- or STAT3-TB2-expressing cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. n Quantification of Liperfluo fluorescence intensity showing lipid peroxidation in HA-STAT3-WT- or HA-STAT3-TB2-expressing cells after DMSO or FLLL32 treatment. o Quantification of relative EdU-positive cells in HCT116 cells expressing HA-STAT3-WT or HA-STAT3-TB2 after DMSO or FLLL32 treatment. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Lamin B1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+b1/Lamin+B1+Antibody/pm42863326-207-55-79
    Average 96 stars, based on 1 article reviews
    lamin b1 - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    Image Search Results


    FLLL32 targets STAT3 to suppress pSTAT3-mediated GPX4/SLC40A1 transcriptional regulation. a Western blot analysis of pSTAT3, total STAT3, and β-Tubulin in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. b Nuclear-cytoplasmic fractionation analysis of pSTAT3 and STAT3 in HCT116 cells treated with DMSO or FLLL32. Lamin B1 and β-Tubulin were used as nuclear and cytoplasmic markers. c Predicted STAT3-binding motif within the GPX4 and SLC40A1 promoter regions. d , e ChIP-qPCR analysis of pSTAT3 occupancy at GPX4 and SLC40A1 promoter regions in HCT116 cells treated with DMSO or FLLL32. IgG was used as the negative control. f , g Promoter luciferase assays showing the activity of wild-type or mutant GPX4 and SLC40A1 promoters in cells transfected with control or STAT3 expression plasmids. h , i Dual-luciferase reporter assays of wild-type or STAT3-binding motif-mutant GPX4 (h) and SLC40A1 (i) promoter reporters in STAT3-overexpressing cells after DMSO or FLLL32 treatment. Mut indicates mutation of the predicted STAT3-binding motif. j Molecular docking model showing the predicted interaction between FLLL32 and STAT3 at Glu638 and Met726. k , l Bio-layer interferometry analysis of the binding between FLLL32 and purified STAT3-WT and STAT3-TB2 proteins. m Western blot analysis of HA, pSTAT3, SLC40A1, GPX4 in STAT3-WT- or STAT3-TB2-expressing cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. n Quantification of Liperfluo fluorescence intensity showing lipid peroxidation in HA-STAT3-WT- or HA-STAT3-TB2-expressing cells after DMSO or FLLL32 treatment. o Quantification of relative EdU-positive cells in HCT116 cells expressing HA-STAT3-WT or HA-STAT3-TB2 after DMSO or FLLL32 treatment. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Journal: Molecular Biomedicine

    Article Title: Curcumin analogue FLLL32 induces ferroptosis by targeting the STAT3-GPX4/SLC40A1 axis in colorectal cancer

    doi: 10.1186/s43556-026-00575-5

    Figure Lengend Snippet: FLLL32 targets STAT3 to suppress pSTAT3-mediated GPX4/SLC40A1 transcriptional regulation. a Western blot analysis of pSTAT3, total STAT3, and β-Tubulin in HCT116 and HT29 cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. b Nuclear-cytoplasmic fractionation analysis of pSTAT3 and STAT3 in HCT116 cells treated with DMSO or FLLL32. Lamin B1 and β-Tubulin were used as nuclear and cytoplasmic markers. c Predicted STAT3-binding motif within the GPX4 and SLC40A1 promoter regions. d , e ChIP-qPCR analysis of pSTAT3 occupancy at GPX4 and SLC40A1 promoter regions in HCT116 cells treated with DMSO or FLLL32. IgG was used as the negative control. f , g Promoter luciferase assays showing the activity of wild-type or mutant GPX4 and SLC40A1 promoters in cells transfected with control or STAT3 expression plasmids. h , i Dual-luciferase reporter assays of wild-type or STAT3-binding motif-mutant GPX4 (h) and SLC40A1 (i) promoter reporters in STAT3-overexpressing cells after DMSO or FLLL32 treatment. Mut indicates mutation of the predicted STAT3-binding motif. j Molecular docking model showing the predicted interaction between FLLL32 and STAT3 at Glu638 and Met726. k , l Bio-layer interferometry analysis of the binding between FLLL32 and purified STAT3-WT and STAT3-TB2 proteins. m Western blot analysis of HA, pSTAT3, SLC40A1, GPX4 in STAT3-WT- or STAT3-TB2-expressing cells treated with DMSO or FLLL32. β-Tubulin was used as the loading control. n Quantification of Liperfluo fluorescence intensity showing lipid peroxidation in HA-STAT3-WT- or HA-STAT3-TB2-expressing cells after DMSO or FLLL32 treatment. o Quantification of relative EdU-positive cells in HCT116 cells expressing HA-STAT3-WT or HA-STAT3-TB2 after DMSO or FLLL32 treatment. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Article Snippet: Primary antibodies used for immunoblot analysis were as follows: β-Tubulin (ZSGB-BIO, TA347064), STAT3 (HUABIO, ET1603-40), Phospho-STAT3 (Tyr705) (CST, 9145S), GPX4 (HUABIO, ET1706-45), SLC40A1 (CST, 80672T), FTH1 (Abclonal, A1144), FTL (Abclonal, A1768), Lamin B1 (Abclonal, A11495), E-cadherin (Proteintech, 20874-1-AP), N-cadherin (Proteintech, 22018-1-AP), Vimentin (Proteintech, 10366-1-AP), and HA-tag (Proteintech, 66006-2-Ig).

    Techniques: Western Blot, Analysis, Control, Fractionation, Binding Assay, ChIP-qPCR, Negative Control, Luciferase, Activity Assay, Mutagenesis, Transfection, Expressing, Docking Assay, Purification, Fluorescence