alair1  (R&D Systems)

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Bioinformatic analysis of LAIR1 expression in pan-cancer and its prognostic value in GC. (A) LAIR1 expression across cancers from TIMER2. (B-D) Poor prognosis associated with high LAIR1 expression (probe 208071_s_at) in GC: (B) OS, (C) FPS, (D) PPS. (E-G) Poor prognosis associated with high LAIR1 expression (probe 210644_s_at) in GC: (E) OS, (F) FPS, (G) PPS. *, P<0.05; ***, P<0.001. CI, confidence interval; FPS, first progression survival; GC, gastric cancer; HR, hazard ratio; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; OS, overall survival; PPS, post-progression survival; TPM, transcripts per million.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Expressing

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Expression and prognostic analysis of LAIR1 in GC. (A) Representative images showing low LAIR1 expression in normal gastric tissue and high expression in GC tissue. Negative staining (IHC scores 1–2), weak staining (IHC scores 3–4), moderate staining (IHC scores 6–9), and strong staining (IHC scores 12). (B) OS based on LAIR1 expression status. (C) PFS based on LAIR1 expression status. (D) LAIR1 protein expression in 16 paired GC tissues (N: adjacent normal; T: tumor). (E) LAIR1 mRNA levels in the same 16 paired tissues. ****, P<0.0001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, gastric cancer; IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; mRNA, messenger RNA; OS, overall survival; PFS, progression-free survival.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Expressing, Negative Staining, Staining, Immunohistochemistry

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: LAIR1 expression and its impact on GC cell proliferation. (A) LAIR1 protein levels in various GC cell lines. (B) Statistical analysis of LAIR1 expression across cell lines. (C) Validation of LAIR1 knockdown efficiency via lentivirus in MGC803 cells. (D) Validation of LAIR1 overexpression efficiency in MKN45 cells. (E) Effect of LAIR1 knockdown on cell viability (CCK-8). (F) Effect of LAIR1 overexpression on cell viability (CCK-8). (G) Colony formation after LAIR1 knockdown in MGC803 cells (crystal violet staining). (H) Colony formation after LAIR1 overexpression in MKN45 cells (crystal violet staining). **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. CCK-8, Cell Counting Kit-8; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, gastric cancer; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; sh-1, shLAIR1-1; sh-2, shLAIR1-2; shLAIR1, short hairpin RNA targeting LAIR1.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Expressing, Biomarker Discovery, Knockdown, Over Expression, CCK-8 Assay, Staining, Cell Counting, Negative Control, shRNA

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Effect of LAIR1 on GC cell migration and invasion. (A) Scratch wound assay in LAIR1-knockdown MGC803 cells. (B) Scratch wound assay in LAIR1-overexpressing MKN45 cells. (C) Transwell invasion assay in LAIR1-knockdown MGC803 cells (crystal violet staining). (D) Transwell invasion assay in LAIR1-overexpressing MKN45 cells (crystal violet staining). ***, P<0.001; ****, P<0.0001. GC, gastric cancer; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; sh-1, shLAIR1-1; sh-2, shLAIR1-2; shLAIR1, short hairpin RNA targeting LAIR1.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Migration, Scratch Wound Assay Assay, Knockdown, Transwell Invasion Assay, Staining, Negative Control, shRNA

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Effect of LAIR1 knockdown on subcutaneous tumor growth in nude mice. (A) Tumorigenesis in nude mice following LAIR1 downregulation. (B,C) Tumor progression: significant differences in subcutaneous tumor growth curves (B) and final tumor weights (C). (D) IHC scores for LAIR1 and Ki-67. (E) Representative IHC images for LAIR1. (F) Representative IHC images for Ki-67. **, P<0.01; ****, P<0.0001. IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Knockdown, Immunohistochemistry, Negative Control, shRNA

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Effect of LAIR1 knockdown on peritoneal metastasis in nude mice. (A) Representative images of peritoneal metastatic nodules. (B) The number of nodules in the peritoneal metastases of nude mice exhibited significant variations. (C) IHC scores for LAIR1, Snail1, N-cadherin, and E-cadherin. (D-G) Representative IHC images for LAIR1 (D), Snail1 (E), N-cadherin (F), and E-cadherin (G). *, P<0.05; **, P<0.01; ****, P<0.0001. IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Knockdown, Immunohistochemistry, Negative Control, shRNA

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Effect of LAIR1 knockdown on lung and lymph node metastasis in nude mice. (A) Gross view of lung metastasis. (B) Lung metastatic nodules visualized by H&E staining. (C) Quantitative analysis of metastatic lung nodules. (D) Gross view of lymph node metastasis. (E) Quantitative analysis of metastatic lymph nodes. **, P<0.01; ****, P<0.0001. H&E, hematoxylin-eosin; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Knockdown, Staining, Negative Control, shRNA

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Bioinformatic analysis of LAIR1 expression in pan-cancer and its prognostic value in GC. (A) LAIR1 expression across cancers from TIMER2. (B-D) Poor prognosis associated with high LAIR1 expression (probe 208071_s_at) in GC: (B) OS, (C) FPS, (D) PPS. (E-G) Poor prognosis associated with high LAIR1 expression (probe 210644_s_at) in GC: (E) OS, (F) FPS, (G) PPS. *, P<0.05; ***, P<0.001. CI, confidence interval; FPS, first progression survival; GC, gastric cancer; HR, hazard ratio; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; OS, overall survival; PPS, post-progression survival; TPM, transcripts per million.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Expressing

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Expression and prognostic analysis of LAIR1 in GC. (A) Representative images showing low LAIR1 expression in normal gastric tissue and high expression in GC tissue. Negative staining (IHC scores 1–2), weak staining (IHC scores 3–4), moderate staining (IHC scores 6–9), and strong staining (IHC scores 12). (B) OS based on LAIR1 expression status. (C) PFS based on LAIR1 expression status. (D) LAIR1 protein expression in 16 paired GC tissues (N: adjacent normal; T: tumor). (E) LAIR1 mRNA levels in the same 16 paired tissues. ****, P<0.0001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, gastric cancer; IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; mRNA, messenger RNA; OS, overall survival; PFS, progression-free survival.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Expressing, Negative Staining, Staining, Immunohistochemistry

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: LAIR1 expression and its impact on GC cell proliferation. (A) LAIR1 protein levels in various GC cell lines. (B) Statistical analysis of LAIR1 expression across cell lines. (C) Validation of LAIR1 knockdown efficiency via lentivirus in MGC803 cells. (D) Validation of LAIR1 overexpression efficiency in MKN45 cells. (E) Effect of LAIR1 knockdown on cell viability (CCK-8). (F) Effect of LAIR1 overexpression on cell viability (CCK-8). (G) Colony formation after LAIR1 knockdown in MGC803 cells (crystal violet staining). (H) Colony formation after LAIR1 overexpression in MKN45 cells (crystal violet staining). **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. CCK-8, Cell Counting Kit-8; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, gastric cancer; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; sh-1, shLAIR1-1; sh-2, shLAIR1-2; shLAIR1, short hairpin RNA targeting LAIR1.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Expressing, Biomarker Discovery, Knockdown, Over Expression, CCK-8 Assay, Staining, Cell Counting, Negative Control, shRNA

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Effect of LAIR1 on GC cell migration and invasion. (A) Scratch wound assay in LAIR1-knockdown MGC803 cells. (B) Scratch wound assay in LAIR1-overexpressing MKN45 cells. (C) Transwell invasion assay in LAIR1-knockdown MGC803 cells (crystal violet staining). (D) Transwell invasion assay in LAIR1-overexpressing MKN45 cells (crystal violet staining). ***, P<0.001; ****, P<0.0001. GC, gastric cancer; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; sh-1, shLAIR1-1; sh-2, shLAIR1-2; shLAIR1, short hairpin RNA targeting LAIR1.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Migration, Scratch Wound Assay Assay, Knockdown, Transwell Invasion Assay, Staining, Negative Control, shRNA

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Effect of LAIR1 knockdown on subcutaneous tumor growth in nude mice. (A) Tumorigenesis in nude mice following LAIR1 downregulation. (B,C) Tumor progression: significant differences in subcutaneous tumor growth curves (B) and final tumor weights (C). (D) IHC scores for LAIR1 and Ki-67. (E) Representative IHC images for LAIR1. (F) Representative IHC images for Ki-67. **, P<0.01; ****, P<0.0001. IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Knockdown, Immunohistochemistry, Negative Control, shRNA

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Effect of LAIR1 knockdown on peritoneal metastasis in nude mice. (A) Representative images of peritoneal metastatic nodules. (B) The number of nodules in the peritoneal metastases of nude mice exhibited significant variations. (C) IHC scores for LAIR1, Snail1, N-cadherin, and E-cadherin. (D-G) Representative IHC images for LAIR1 (D), Snail1 (E), N-cadherin (F), and E-cadherin (G). *, P<0.05; **, P<0.01; ****, P<0.0001. IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Knockdown, Immunohistochemistry, Negative Control, shRNA

Journal: Translational Cancer Research

Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

doi: 10.21037/tcr-2026-1-0136

Figure Lengend Snippet: Effect of LAIR1 knockdown on lung and lymph node metastasis in nude mice. (A) Gross view of lung metastasis. (B) Lung metastatic nodules visualized by H&E staining. (C) Quantitative analysis of metastatic lung nodules. (D) Gross view of lymph node metastasis. (E) Quantitative analysis of metastatic lymph nodes. **, P<0.01; ****, P<0.0001. H&E, hematoxylin-eosin; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

Techniques: Knockdown, Staining, Negative Control, shRNA

Journal: JCI Insight

Article Title: LAIR1 prevents excess inflammatory tissue damage in Staphylococcus aureus skin infection and cutaneous T cell lymphoma

doi: 10.1172/jci.insight.183935

Figure Lengend Snippet: ( A – C ) CD4 + cells were isolated from CTCL patient peripheral blood and analyzed by single cell RNA-Seq. UMAP projections show 92,496 mononuclear cells from 16 peripheral blood samples from 6 patients with CTCL across multiple time points. ( A ) Cell type assignment by highest spearman rho of purified immune populations in the Human Primary Cell Atlas. ( B and C ) UMAP plots show expression of LAIR1 ( B ) and LAIR2 ( C ). ( D ) Illustration depicts hypothesis that LAIR2 overexpression reduces LAIR1 signaling in CTCL. ( E ) Illustration depicts mouse model for loss of LAIR1 signaling via KO of Lair1 .

Article Snippet: WT C57BL/6J and Lair1 –/– (B6.Cg-Lair1tm1.1Jco/J, JAX Strain #:032788) mice ( ) were purchased from The Jackson Laboratory and housed in a pathogen-free environment in the WUSM animal facility.

Techniques: Isolation, Single Cell, RNA Sequencing, Purification, Expressing, Over Expression

Journal: JCI Insight

Article Title: LAIR1 prevents excess inflammatory tissue damage in Staphylococcus aureus skin infection and cutaneous T cell lymphoma

doi: 10.1172/jci.insight.183935

Figure Lengend Snippet: WT and Lair1- KO mice were infected s.c. with 1 × 10 7 CFU S . aureus USA300 LAC. ( A and B ) Lesions were monitored over 14 days and quantified as abscess ( A ) and dermonecrosis ( B ) area (noninvasive measurements, area calculated: A = ([pi/2] × l × w). Statistical analysis by repeated measures 2-way ANOVA with Bonferroni post hoc tests. Results are representative of 5 independent experiments ( n = 40 WT, n = 40 Lair1 KO). ( C ) Representative gross images of S . aureus skin lesions in WT (left) and Lair1 -KO (right) mice on 4dpi. Abscess boundaries are indicated with the dashed lines. Dermonecrosis is the central erythematous lesion. ( D ) Representative H&E stains of lesions at 2 dpi. Scale bars: 1 mm. Asterisks indicate abscesses, and solid lines indicate areas of dermonecrosis, which are larger in Lair1 KO.

Article Snippet: WT C57BL/6J and Lair1 –/– (B6.Cg-Lair1tm1.1Jco/J, JAX Strain #:032788) mice ( ) were purchased from The Jackson Laboratory and housed in a pathogen-free environment in the WUSM animal facility.

Techniques: Infection

Journal: JCI Insight

Article Title: LAIR1 prevents excess inflammatory tissue damage in Staphylococcus aureus skin infection and cutaneous T cell lymphoma

doi: 10.1172/jci.insight.183935

Figure Lengend Snippet: ( A – F ) Skin lesions were collected at 18 hours after infection, homogenized, and cytokine production measured by multiplex cytokine array. ( A ) Heatmap shows average WT and Lair1 KO z -scored cytokine concentrations. ( B – F ) Bar graphs show all cytokines with significantly different expression between WT and Lair1 KO. ( G – L ) BMDMs were generated in vitro and treated with PBS or S . aureus at a multiplicity of infection (MOI) of 10:1 for 18 hours. Cell supernatant was analyzed by multiplex cytokine array. ( G ) Heatmap shows average WT and Lair1- KO cytokine concentrations in PBS and S . aureus groups. ( H – L ) Bar graphs show all cytokines with significantly different expression between WT and Lair1 KO ( n = 2 independent infections, 2–6 mice per group). ( B – F and G – L ) Two-way ANOVA with Fisher’s LSD for multiple comparisons. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: WT C57BL/6J and Lair1 –/– (B6.Cg-Lair1tm1.1Jco/J, JAX Strain #:032788) mice ( ) were purchased from The Jackson Laboratory and housed in a pathogen-free environment in the WUSM animal facility.

Techniques: Infection, Multiplex Assay, Expressing, Generated, In Vitro

Journal: JCI Insight

Article Title: LAIR1 prevents excess inflammatory tissue damage in Staphylococcus aureus skin infection and cutaneous T cell lymphoma

doi: 10.1172/jci.insight.183935

Figure Lengend Snippet: ( A and C ) BMDM from WT and Lair1-KO mice were treated with PBS or PAM3CSK4 for 6 hours and were then subjected to RNA-Seq. ( B and D ) Skin punch biopsies from WT and Lair1 -KO mouse S . aureus skin infections were taken at 2 dpi and were then subjected to RNA-Seq. GSEA was used to define the top 20 mouse Reactome pathways with enrichment scores and nominal P value in genes upregulated in Lair1 KO in BMDM treated with PAM3CSK4 ( A ) and 2 dpi SSTI ( B ); collagen-related pathways common to both analyses are bolded in teal. Genes from the 4 pathways, many of which overlapped, were classified into 3 general categories: collagen genes, collagen synthesis genes, and extracellular matrix (ECM) remodeling genes. Heatmaps show z -scored expression for these genes for BMDM ( C ) and S . aureus SSTI ( D ). ( E and F ) BMDM from WT and Lair1 -KO mice were treated with PBS or PAM3CSK4 for 24 hours and were then fixed and stained for Serpin H1. Representative images from 3 independent experiments are shown ( E ). Scale bars: 50 μm. Images were analyzed in CellProfiler and mean Serpin H1 cytoplasmic fluorescence intensity per cell was calculated ( F ). Mean ± SEM are indicated by black bars. Statistical analysis was done by 2-way ANOVA with multiple comparisons.

Article Snippet: WT C57BL/6J and Lair1 –/– (B6.Cg-Lair1tm1.1Jco/J, JAX Strain #:032788) mice ( ) were purchased from The Jackson Laboratory and housed in a pathogen-free environment in the WUSM animal facility.

Techniques: RNA Sequencing, Expressing, Staining, Fluorescence

Journal: JCI Insight

Article Title: LAIR1 prevents excess inflammatory tissue damage in Staphylococcus aureus skin infection and cutaneous T cell lymphoma

doi: 10.1172/jci.insight.183935

Figure Lengend Snippet: WT and Lair1- KO neutrophils were isolated from mouse bone marrow. ( A ) Amplex red fluorescence measured by plate reader indicates hydrogen peroxide (H 2 O 2 ) production in neutrophils treated with PBS or S . aureus at MOI 50:1 for 30 minutes. ( B ) Ratio of WT/KO lysosome probe MFI measured by flow cytometry indicates lysosome acidification in neutrophils treated with PBS or S . aureus at MOI 100:1 for 30 minutes. ( C ) Neutrophils incubated for 30 minutes with PBS or S . aureus bioparticles (pHrodo) that fluoresce when acidified in the lysosome were subjected to flow cytometry. ( D ) Neutrophils incubated for 30 minutes with PBS or mCherry-expressing S . aureus at MOI 50:1 were subjected to flow cytometry. mCherry MFI is shown as a ratio of KO to WT. Statistical analysis for all panels was done by 2-way ANOVA with Fisher’s LSD. ** P < 0.01.

Article Snippet: WT C57BL/6J and Lair1 –/– (B6.Cg-Lair1tm1.1Jco/J, JAX Strain #:032788) mice ( ) were purchased from The Jackson Laboratory and housed in a pathogen-free environment in the WUSM animal facility.

Techniques: Isolation, Fluorescence, Flow Cytometry, Incubation, Expressing