l6 cells (ATCC)

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ATCC l6 cells
L6 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 922 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l6+cells/L6/us12636316-123-0-2
Average 96 stars, based on 922 article reviews

l6 cells - by Bioz Stars, 2026-09

96/100 stars

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Centrifugation:

Article Title: Mitochondrial Transplantation Restores Immune Cell Metabolism in Sepsis: A Metabolomics Study
Article Snippet: .. Briefly, L6 cells (ATCC; CRL-1458, Manassas, VA, USA) were homogenized using a 26G syringe in SHE buffer (0.25 M sucrose, 20 mM HEPES [pH 7.4], 2 mM EGTA, 10 mM KCl, 1.5 mM MgCl 2 , and 0.1% defatted BSA with protease inhibitors), followed by centrifugation at 1500× g for 5 min at 4 °C. ..

Derivative Assay:

Article Title: Injection composition containing isolated mitochondria, and use thereof
Article Snippet: A Chinese hamster ovary-derived cell line (i.e., CHO cells) were inoculated in the Dulbecco's modified eagle's medium (DMEM, Gibco) medium containing 10% fetal bovine serum (FBS, Gibco) and cultured for 72 hours. .. L6 cells (American Type Culture Collection, ATCC, CRL-1458), which are a myoblast cell line derived from rat skeletal muscle, were inoculated into Dulbecco's modified eagle's medium-high glucose (DMEM-high glucose) containing 10% fetal bovine serum (FBS, Gibco), and cultured for 72 hours. .. After the respective culture of cells were completed, respective cells were washed twice using Dulbecco's phosphate buffered saline (DPBS, Gibco).

Article Title: Pharmaceutical composition for preventing or treating myositis, comprising isolated mitochondria as active ingredient
Article Snippet: .. L6 cells (American Type Culture Collection, ATCC, CRL-1458), a myoblast cell line derived from skeletal muscle of rats, were inoculated into a DMEM-High glucose (Dulbecco's modified eagle's medium-High glucose, Gibco) medium containing 10% fetal bovine serum (FBS, Gibco) and cultured for 72 hours. ..

Modification:

Cell Culture:

In Vitro:

Article Title: Semisynthetic Derivatives of Perovskone: Development of a Promising Class of Antiprotozoal Lead Compounds.
Article Snippet: Perovskones, intricate triterpenoids with potent antiplasmodial activity, predominantly derive from Salvia hydrangea DC. ex Benth.. In this study, ample quantities of the parent compound, perovskone (1), were isolated from the plant.. Using perovskone (1) as a feedstock, seven semisynthetic analogues (2−8) were generated via reactions like hydroxylation, elimination, and esterification.

Activity Assay:

Staining:

Article Title: Pharmaceutical composition comprising isolated mitochondria for preventing or treating tendinopathy
Article Snippet: .. To identify whether foreign mitochondria are delivered into tendon tissue, first, L6 cells (ATCC, CRL-1458) were stained using Mitotracker CMXRos Red dye before isolation of foreign healthy mitochondria. ..

Isolation:

Transfection:

Article Title: Polynucleotides encoding human beta klotho antibodies or binding fragments thereof and methods of use thereof
Article Snippet: The transfecting plasmids consisted of two reporter plasmids Gal4-Elk1 and 5×UAS-Luc (Agilent Technologies PathDetect Elk1 trans-reporting system Cat #219005), and plasmids encoding human beta klotho (GeneCopoeia Cat #EX-E1104-MO2) or cynomolgus monkey beta klotho (cyno beta klotho) and human FGFR1c (GeneCopoeia Cat #EX-A0260-MO2). .. For example, ELK1-luciferase reporter assays, which measure FGFRIc/beta klotho signaling, were performed using transiently transfected HEK293, HEK293T, or L6 cells (ATCC). .. The transfecting plasmids consisted of two reporter plasmids Gal4-Elk1 and 5×UAS-Luc (Agilent Technologies PathDetect Elk1 trans-reporting system Cat #219005), and plasmids encoding human beta klotho (GeneCopoeia Cat #EX-E1104-MO2) or cynomolgus monkey beta klotho (cyno beta klotho) and human FGFR1c (GeneCopoeia Cat #EX-A0260-MO2).

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