Journal: Investigative Ophthalmology & Visual Science
Article Title: Deletion of Fgfr2 in Ductal Basal Epithelium With Tamoxifen Induces Obstructive Meibomian Gland Dysfunction
doi: 10.1167/iovs.65.13.36
Figure Lengend Snippet: Active differentiation of Krt5 + lineage in ductal MG and conjunctival epithelium after TAM-induced Fgfr2 deletion. ( A ) Krt5CreERT2, Fgfr2 flox/flox mice were crossed with Rosa mTmG reporter strains to delineate the Krt5 + basal meibocytes and its progeny after inducible deletion of Fgfr2 by TAM . ( B ) Experimental scheme to examine the morphological changes and EGFP expression of Krt5 lineage at day 10. ( C – D1 ) MGs in tarsal plates. Representative images of MGs under bright field ( C, D ) and with EGFP fluorescence ( green ) ( C1, D1 ) from the control mice ( Krt5 mTmG ) ( C, C1 ) and Fgfr2 CKO -mTmG ( D, D1 ) after a 10-day chase. Extensive lipid stagnations (bright spots in D ) with MG atrophy and ductal dilation (indicated by arrowheads in D1 ) were seen in Fgfr2 CKO -mTmG . ( E – H1 ) Expression of EGFP ( green ) and Tomato ( red ) in MGs. Sagittal sections ( E, F ) and cross-sections ( G, H ) of MGs with corresponding DAPI counterstaining ( E1, F1, G1, H1 ) showed that EGFP was expressed in the acinar and ductal basal cells in control mice, but was expressed in the hyper-stratified ductal epithelium obliterating the MG central ducts of the Fgfr2 CKO -mTmG . White dotted lines indicate MG area and yellow dotted lines indicate duct area. When compared with the controls ( E, G ), enhanced EGFP expression ( arrowheads in F and H ) was also noted in the conjunctival epithelium of the Fgfr2 CKO -mTmG mice. MG, Meibomian gland; a, acini; d, duct; conj, conjunctiva; HF, hair follicle. Scale bars : 100 µm.
Article Snippet: The Krt5CreERT2; Rosa mTmG reporter mice (referred to as Krt5 mTmG mice) was generated by crossing the Krt5CreERT2 mice (JAX stock no. 029155) with the Rosa mTmG mice (JAX stock#007676) to visualize the Cre recombination in MGs and other ocular surface tissues with TAM induction.
Techniques: Expressing, Fluorescence, Control
