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HAVcR-1 overexpression in PZ-HPV-7 prostate cancer cell line. ( A ) Confirmation of gene knockdown via qPCR. ( B ) Protein expression validation of PZ-HPV-7 pEF6 and PZ-HPV-7 HAVCR-1EXP. Images show fluorescence emission at 100× magnification correlating to HAVcR-1 expression or nuclear staining and a merged image of both. Images are representative of three independent experiments. Scale bars represent 20 µm. Graph shows quantitative analysis of immunofluorescent staining of HAVcR-1 (mean + SEM, n = 3). ( C , D ) Protein expression and phosphorylation significantly increased with HAVcR-1 overexpression. Protein was extracted from PZ-HPV-7 pEF6 and PZ-HPV-7HAVcR-1 EXP and sent to Kinex Bioinformatics for a Kinex ™ antibody <t>microarray.</t> Graphs show the percentage change from control of protein phosphorylation or total protein expression for all significantly increased results (z value ≥ 1.65). ( E , F ) Protein expression and phosphorylation significantly decreased with HAVcR-1 overexpression, (z value ≤ −1.65). ( G ) β-Catenin Y333 signalling changes with Kinex™ Antibody Microarray, showing fold change from PZ-HPV-7 pEF6 control of all proteins and phosphosites involved with β-catenin signalling included within the Kinex™ antibody microarray. * represents p ≤ 0.05.
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HAVcR-1 overexpression in PZ-HPV-7 prostate cancer cell line. ( A ) Confirmation of gene knockdown via qPCR. ( B ) Protein expression validation of PZ-HPV-7 pEF6 and PZ-HPV-7 HAVCR-1EXP. Images show fluorescence emission at 100× magnification correlating to HAVcR-1 expression or nuclear staining and a merged image of both. Images are representative of three independent experiments. Scale bars represent 20 µm. Graph shows quantitative analysis of immunofluorescent staining of HAVcR-1 (mean + SEM, n = 3). ( C , D ) Protein expression and phosphorylation significantly increased with HAVcR-1 overexpression. Protein was extracted from PZ-HPV-7 pEF6 and PZ-HPV-7HAVcR-1 EXP and sent to Kinex Bioinformatics for a Kinex ™ antibody microarray. Graphs show the percentage change from control of protein phosphorylation or total protein expression for all significantly increased results (z value ≥ 1.65). ( E , F ) Protein expression and phosphorylation significantly decreased with HAVcR-1 overexpression, (z value ≤ −1.65). ( G ) β-Catenin Y333 signalling changes with Kinex™ Antibody Microarray, showing fold change from PZ-HPV-7 pEF6 control of all proteins and phosphosites involved with β-catenin signalling included within the Kinex™ antibody microarray. * represents p ≤ 0.05.

Journal: Biomolecules

Article Title: Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity

doi: 10.3390/biom12020338

Figure Lengend Snippet: HAVcR-1 overexpression in PZ-HPV-7 prostate cancer cell line. ( A ) Confirmation of gene knockdown via qPCR. ( B ) Protein expression validation of PZ-HPV-7 pEF6 and PZ-HPV-7 HAVCR-1EXP. Images show fluorescence emission at 100× magnification correlating to HAVcR-1 expression or nuclear staining and a merged image of both. Images are representative of three independent experiments. Scale bars represent 20 µm. Graph shows quantitative analysis of immunofluorescent staining of HAVcR-1 (mean + SEM, n = 3). ( C , D ) Protein expression and phosphorylation significantly increased with HAVcR-1 overexpression. Protein was extracted from PZ-HPV-7 pEF6 and PZ-HPV-7HAVcR-1 EXP and sent to Kinex Bioinformatics for a Kinex ™ antibody microarray. Graphs show the percentage change from control of protein phosphorylation or total protein expression for all significantly increased results (z value ≥ 1.65). ( E , F ) Protein expression and phosphorylation significantly decreased with HAVcR-1 overexpression, (z value ≤ −1.65). ( G ) β-Catenin Y333 signalling changes with Kinex™ Antibody Microarray, showing fold change from PZ-HPV-7 pEF6 control of all proteins and phosphosites involved with β-catenin signalling included within the Kinex™ antibody microarray. * represents p ≤ 0.05.

Article Snippet: In preparation for a Kinexus™ Antibody Microarray, PZ-HPV-7 pEF6 and PZ-HPV-7 HAVcR1EXP cells were cultured in 10 cm dishes.

Techniques: Over Expression, Expressing, Fluorescence, Staining, Microarray