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kam-2000 kinexus antibody microarrays  (Kinexus Bioinformatics Corporation)

 
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    Kinexus Bioinformatics Corporation kam-2000 kinexus antibody microarrays
    Kam 2000 Kinexus Antibody Microarrays, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kinexus+antibody+microarray/kam+2000+array/pm37883333-224-2-3
    Average 90 stars, based on 1 article reviews
    kam-2000 kinexus antibody microarrays - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Mammalian cells internalize bacteriophages and use them as a resource to enhance cellular growth and survival
    Article Snippet: The MDCK-I samples were then incubated on the KAM-1325 array before sending the array to Kinexus for analysis, while the A549 samples were sent directly to Kinexus for analysis on the KAM-2000 array.

    Article Title: Mammalian cells internalize bacteriophages and use them as a resource to enhance cellular growth and survival.
    Article Snippet: We used 2 protein microarrays from Kinexus Biotech, the KAM-1325 microarray that contains 1,325 pan- and phosphosite-specific antibodies covering all the main cellular signaling pathways, and the KAM-2000 microarray with 2,000 pan- and phosphosite-specific antibodies.

    Article Title: Mammalian cells internalize bacteriophages and use them as a resource to enhance cellular growth and survival
    Article Snippet: We used 2 protein microarrays from Kinexus Biotech, the KAM-1325 microarray that contains 1,325 pan- and phosphosite-specific antibodies covering all the main cellular signaling pathways, and the KAM-2000 microarray with 2,000 pan- and phosphosite-specific antibodies.

    Article Title: Mammalian cells internalize bacteriophages and use them as a resource to enhance cellular growth and survival.
    Article Snippet: KAM-1325 and KAM-2000 Kinexus antibody microarrays Following the protocol described by Bichet and colleagues [32], confluent MDCK-I or A549 cell lines, for KAM-1325 or KAM-2000 microarrays, respectively, were incubated with T4 phages or Filter control samples for 8 hours at 37 ̊C and 5% CO2.

    Article Title: Mammalian cells internalize bacteriophages and use them as a resource to enhance cellular growth and survival.
    Article Snippet: The MDCK-I samples were then incubated on the KAM-1325 array before sending the array to Kinexus for analysis, while the A549 samples were sent directly to Kinexus for analysis on the KAM-2000 array.

    Microarray:

    Article Title: Glycogen synthase kinase 3 inhibition controls Mycobacterium tuberculosis infection.
    Article Snippet: .. The KAM-2000 microarray featured 1165 pan-specific and 894 phosphosite-specific commercial antibodies, produced principally by Kinexus Bioinformatics (Vancouver, BC,Canada) aswell as fromother suppliers following their in-house validation,witheachantibodyprinted inquadruplicateoneachNexterionP slide (Schott AG, Jena, Germany). ..

    Phospho-proteomics:

    Article Title: Glycogen synthase kinase 3 inhibition controls Mycobacterium tuberculosis infection.
    Article Snippet: .. The KAM-2000 microarray featured 1165 pan-specific and 894 phosphosite-specific commercial antibodies, produced principally by Kinexus Bioinformatics (Vancouver, BC,Canada) aswell as fromother suppliers following their in-house validation,witheachantibodyprinted inquadruplicateoneachNexterionP slide (Schott AG, Jena, Germany). ..

    Produced:

    Article Title: Glycogen synthase kinase 3 inhibition controls Mycobacterium tuberculosis infection.
    Article Snippet: .. The KAM-2000 microarray featured 1165 pan-specific and 894 phosphosite-specific commercial antibodies, produced principally by Kinexus Bioinformatics (Vancouver, BC,Canada) aswell as fromother suppliers following their in-house validation,witheachantibodyprinted inquadruplicateoneachNexterionP slide (Schott AG, Jena, Germany). ..

    Biomarker Discovery:

    Article Title: Glycogen synthase kinase 3 inhibition controls Mycobacterium tuberculosis infection.
    Article Snippet: .. The KAM-2000 microarray featured 1165 pan-specific and 894 phosphosite-specific commercial antibodies, produced principally by Kinexus Bioinformatics (Vancouver, BC,Canada) aswell as fromother suppliers following their in-house validation,witheachantibodyprinted inquadruplicateoneachNexterionP slide (Schott AG, Jena, Germany). ..



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    HAVcR-1 overexpression in PZ-HPV-7 prostate cancer cell line. ( A ) Confirmation of gene knockdown via qPCR. ( B ) Protein expression validation of PZ-HPV-7 pEF6 and PZ-HPV-7 HAVCR-1EXP. Images show fluorescence emission at 100× magnification correlating to HAVcR-1 expression or nuclear staining and a merged image of both. Images are representative of three independent experiments. Scale bars represent 20 µm. Graph shows quantitative analysis of immunofluorescent staining of HAVcR-1 (mean + SEM, n = 3). ( C , D ) Protein expression and phosphorylation significantly increased with HAVcR-1 overexpression. Protein was extracted from PZ-HPV-7 pEF6 and PZ-HPV-7HAVcR-1 EXP and sent to Kinex Bioinformatics for a Kinex ™ antibody <t>microarray.</t> Graphs show the percentage change from control of protein phosphorylation or total protein expression for all significantly increased results (z value ≥ 1.65). ( E , F ) Protein expression and phosphorylation significantly decreased with HAVcR-1 overexpression, (z value ≤ −1.65). ( G ) β-Catenin Y333 signalling changes with Kinex™ Antibody Microarray, showing fold change from PZ-HPV-7 pEF6 control of all proteins and phosphosites involved with β-catenin signalling included within the Kinex™ antibody microarray. * represents p ≤ 0.05.
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    Image Search Results


    HAVcR-1 overexpression in PZ-HPV-7 prostate cancer cell line. ( A ) Confirmation of gene knockdown via qPCR. ( B ) Protein expression validation of PZ-HPV-7 pEF6 and PZ-HPV-7 HAVCR-1EXP. Images show fluorescence emission at 100× magnification correlating to HAVcR-1 expression or nuclear staining and a merged image of both. Images are representative of three independent experiments. Scale bars represent 20 µm. Graph shows quantitative analysis of immunofluorescent staining of HAVcR-1 (mean + SEM, n = 3). ( C , D ) Protein expression and phosphorylation significantly increased with HAVcR-1 overexpression. Protein was extracted from PZ-HPV-7 pEF6 and PZ-HPV-7HAVcR-1 EXP and sent to Kinex Bioinformatics for a Kinex ™ antibody microarray. Graphs show the percentage change from control of protein phosphorylation or total protein expression for all significantly increased results (z value ≥ 1.65). ( E , F ) Protein expression and phosphorylation significantly decreased with HAVcR-1 overexpression, (z value ≤ −1.65). ( G ) β-Catenin Y333 signalling changes with Kinex™ Antibody Microarray, showing fold change from PZ-HPV-7 pEF6 control of all proteins and phosphosites involved with β-catenin signalling included within the Kinex™ antibody microarray. * represents p ≤ 0.05.

    Journal: Biomolecules

    Article Title: Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity

    doi: 10.3390/biom12020338

    Figure Lengend Snippet: HAVcR-1 overexpression in PZ-HPV-7 prostate cancer cell line. ( A ) Confirmation of gene knockdown via qPCR. ( B ) Protein expression validation of PZ-HPV-7 pEF6 and PZ-HPV-7 HAVCR-1EXP. Images show fluorescence emission at 100× magnification correlating to HAVcR-1 expression or nuclear staining and a merged image of both. Images are representative of three independent experiments. Scale bars represent 20 µm. Graph shows quantitative analysis of immunofluorescent staining of HAVcR-1 (mean + SEM, n = 3). ( C , D ) Protein expression and phosphorylation significantly increased with HAVcR-1 overexpression. Protein was extracted from PZ-HPV-7 pEF6 and PZ-HPV-7HAVcR-1 EXP and sent to Kinex Bioinformatics for a Kinex ™ antibody microarray. Graphs show the percentage change from control of protein phosphorylation or total protein expression for all significantly increased results (z value ≥ 1.65). ( E , F ) Protein expression and phosphorylation significantly decreased with HAVcR-1 overexpression, (z value ≤ −1.65). ( G ) β-Catenin Y333 signalling changes with Kinex™ Antibody Microarray, showing fold change from PZ-HPV-7 pEF6 control of all proteins and phosphosites involved with β-catenin signalling included within the Kinex™ antibody microarray. * represents p ≤ 0.05.

    Article Snippet: In preparation for a Kinexus™ Antibody Microarray, PZ-HPV-7 pEF6 and PZ-HPV-7 HAVcR1EXP cells were cultured in 10 cm dishes.

    Techniques: Over Expression, Expressing, Fluorescence, Staining, Microarray