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kim 1  (R&D Systems)


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    Structured Review

    R&D Systems kim 1
    Kim 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kim+1/Mouse+TIM-1%2FKIM-1%2FHAVCR+Antibody/pm42777801-55-2-4
    Average 96 stars, based on 298 article reviews
    kim 1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312.
    Article Snippet: .. Samples were blocked for 1 h at room temperature in blocking buffer 5% BSA in PBS with 0.1% Triton X-100, and incubated overnight at 4 °C with primary antibodies against DUSP26 (1:100; Invitrogen, PA5-22013), KIM-1 (1:200; R&D Systems, AF1817), and Lotus tetragonolobus lectin (LTL; 1:100; Vector Laboratories, RFL-1321). .. After washing, samples were incubated for 1 h at room temperature with species-appropriate secondary antibodies (Abcam, 1:400).

    Article Title: DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312
    Article Snippet: .. Samples were blocked for 1 h at room temperature in blocking buffer 5% BSA in PBS with 0.1% Triton X-100, and incubated overnight at 4 °C with primary antibodies against DUSP26 (1:100; Invitrogen, PA5-22013), KIM-1 (1:200; R&D Systems, AF1817), and Lotus tetragonolobus lectin (LTL; 1:100; Vector Laboratories, RFL-1321). .. After washing, samples were incubated for 1 h at room temperature with species-appropriate secondary antibodies (Abcam, 1:400).

    Article Title: DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312.
    Article Snippet: .. After blocking with 5% non-fat milk, membranes were incubated overnight at 4°C with primary antibodies (all diluted at 1:1000) against DUSP26 (Invitrogen, PA5-22013), p53 (Cell Signaling Technology, 2524S), p-p53 (S15) (Cell Signaling Technology, 9284S), p-p53 (S20) (Cell Signaling Technology, 9287T), p-p53 (S46) (Proteintech, 28960), cleaved caspase-3 (Cell Signaling Technology, 9664S), NGAL (R&D Systems, AF1857), KIM-1 (R&D Systems, AF1817), GAPDH (Proteintech, 10494), β-actin (Proteintech, 66009), DNMT1 (Cell Signaling Technology, 5032), DNMT3a (abcam, ab188470), DNMT3b (abcam, ab2851) and FLAG (Sigma-Aldrich, 102698217), as appropriate. .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (1:5000; Proteintech).

    Article Title: DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312
    Article Snippet: .. After blocking with 5% non-fat milk, membranes were incubated overnight at 4 °C with primary antibodies (all diluted at 1:1000) against DUSP26 (Invitrogen, PA5-22013), p53 (Cell Signaling Technology, 2524S), p-p53 (S15) (Cell Signaling Technology, 9284S), p-p53 (S20) (Cell Signaling Technology, 9287T), p-p53 (S46) (Proteintech, 28960), cleaved caspase-3 (Cell Signaling Technology, 9664S), NGAL (R&D Systems, AF1857), KIM-1 (R&D Systems, AF1817), GAPDH (Proteintech, 10494), β-actin (Proteintech, 66009), DNMT1 (Cell Signaling Technology, 5032), DNMT3a (abcam, ab188470), DNMT3b (abcam, ab2851) and FLAG (Sigma-Aldrich, 102698217), as appropriate. .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (1:5000; Proteintech).

    Incubation:

    Article Title: DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312.
    Article Snippet: .. Samples were blocked for 1 h at room temperature in blocking buffer 5% BSA in PBS with 0.1% Triton X-100, and incubated overnight at 4 °C with primary antibodies against DUSP26 (1:100; Invitrogen, PA5-22013), KIM-1 (1:200; R&D Systems, AF1817), and Lotus tetragonolobus lectin (LTL; 1:100; Vector Laboratories, RFL-1321). .. After washing, samples were incubated for 1 h at room temperature with species-appropriate secondary antibodies (Abcam, 1:400).

    Article Title: DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312
    Article Snippet: .. Samples were blocked for 1 h at room temperature in blocking buffer 5% BSA in PBS with 0.1% Triton X-100, and incubated overnight at 4 °C with primary antibodies against DUSP26 (1:100; Invitrogen, PA5-22013), KIM-1 (1:200; R&D Systems, AF1817), and Lotus tetragonolobus lectin (LTL; 1:100; Vector Laboratories, RFL-1321). .. After washing, samples were incubated for 1 h at room temperature with species-appropriate secondary antibodies (Abcam, 1:400).

    Article Title: DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312.
    Article Snippet: .. After blocking with 5% non-fat milk, membranes were incubated overnight at 4°C with primary antibodies (all diluted at 1:1000) against DUSP26 (Invitrogen, PA5-22013), p53 (Cell Signaling Technology, 2524S), p-p53 (S15) (Cell Signaling Technology, 9284S), p-p53 (S20) (Cell Signaling Technology, 9287T), p-p53 (S46) (Proteintech, 28960), cleaved caspase-3 (Cell Signaling Technology, 9664S), NGAL (R&D Systems, AF1857), KIM-1 (R&D Systems, AF1817), GAPDH (Proteintech, 10494), β-actin (Proteintech, 66009), DNMT1 (Cell Signaling Technology, 5032), DNMT3a (abcam, ab188470), DNMT3b (abcam, ab2851) and FLAG (Sigma-Aldrich, 102698217), as appropriate. .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (1:5000; Proteintech).

    Article Title: Sex-dependent differences in the progression of renal injury and fibrosis following ischemic acute kidney injury
    Article Snippet: Antigen retrieval was performed in DAKO citrate buffer at 95°C for 30 min. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 min. For KIM-1 staining, non-specific binding was blocked using an Avidin/Biotin Blocking Kit (SP-2001, Vector Laboratories, Burlingame, CA, U.S.A.), followed by incubation with blocking serum for 1 h at room temperature. .. Sections were then incubated overnight at 4°C with a rat anti-mouse monoclonal (IgG 2B ) antibody, clone #222414, to detect KIM-1 (MAB1817, 1:500 dilution; R&D Systems, Minneapolis, MN, U.S.A.). .. The following day, the biotinylated secondary antibody was applied using the ECTASTAIN Elite ABC-HRP Kit (PK-6102, Vector Laboratories) according to the manufacturer’s protocol.

    Article Title: DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312
    Article Snippet: .. After blocking with 5% non-fat milk, membranes were incubated overnight at 4 °C with primary antibodies (all diluted at 1:1000) against DUSP26 (Invitrogen, PA5-22013), p53 (Cell Signaling Technology, 2524S), p-p53 (S15) (Cell Signaling Technology, 9284S), p-p53 (S20) (Cell Signaling Technology, 9287T), p-p53 (S46) (Proteintech, 28960), cleaved caspase-3 (Cell Signaling Technology, 9664S), NGAL (R&D Systems, AF1857), KIM-1 (R&D Systems, AF1817), GAPDH (Proteintech, 10494), β-actin (Proteintech, 66009), DNMT1 (Cell Signaling Technology, 5032), DNMT3a (abcam, ab188470), DNMT3b (abcam, ab2851) and FLAG (Sigma-Aldrich, 102698217), as appropriate. .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (1:5000; Proteintech).

    Cell-Signaling:

    Article Title: Gentiopicroside attenuates renal ischemia-reperfusion injury through suppression of JNK/MAPK signaling.
    Article Snippet: Anisomycin (#S7409, purity ≥ 99.93%) was purchased from Selleck. .. Primary antibodies: KIM-1 (#AF1817, R&D Systems, 1:1000); NGAL (#AF1857, R&D Systems, 1:1000); Bax (#50599- 2-Ig, Proteintech, 1:2000); Bcl-2 (#AF6139, Affinity Biosciences, 1:1000); cleaved caspase-3 (#9664, Cell Signaling Technology, 1:1000); JNK1 (#ab110724, Abcam, 1:1000); JNK (#9252, Cell Signaling Technology, 1:1000); phospho-JNK (#4668, Cell Signaling Technology, 1:1000); GPX4 (#sc-166570, Santa Cruz Biotechnology, 1:1000); ACSL4 (#ab155282, Abcam, 1:5000); P62 (#18420-1-AP, Proteintech, 1:10000); LC3 (#14600-1-AP, Proteintech, 1:2000) and β-actin (#A3854, Sigma-Aldrich, 1:40000). .. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) (#C6053), Cell Counting Kit-8 (CCK-8) (#AWC0114), and Annexin V-FITC/PI apoptosis detection kits (#C1062L) were purchased from Baiqiandu Biotechnology, Abiowell, and Beyotime Biotechnology, respectively.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Kidney injury urine biomarker normal ranges in children
    Article Snippet: .. 116 117 Anthropometric, clinical, and laboratory measurements 118 Length (for infante) or height (for older children) and weight were measured using 119 standardized methods to calculate body mass index (BMI), and age and sex-related z-scores 120 Jo rna l P re- pro of 6 for height, weight and BMI using national reference values and the formula: z = [(x/M)L −1]/S 121 × L.22 Automated procedures were used to measure the concentrations of creatinine, 122 cystatin C, CRP, ALT, and CK in serum, as well as urinary creatinine, protein, albumin and 123 glucose. eGFR was calculated using the bedside Schwartz (2009) formula.23 Urinary 124 concentrations of KIM-1 (Quantikine ELISA, R&D system; Minneapolis, MN, USA, Catalog No. 125 DKM100; sensitivity 0.046 ng/ml), NGAL (Quantikine ELISA, R&D system; Minneapolis, MN, 126 USA, Catalog No. DLCN20; sensitivity 0.04 ng/ml), DKK3 (Duo Set ELISA, R&D system; 127 Minneapolis, MN, USA, Catalog No. DY1118, sensitivity 31.2 pg/ml), CHI3L1 (Quantikine 128 ELISA, R&D system; Minneapolis, MN, USA, Catalog No. DC3L10; sensitivity 8.15 pg/ml), 129 MCP-1 (Quantikine ELISA, R&D system; Minneapolis, MN, USA, Catalog No. DCP00; 130 sensitivity 10 pg/ml), PIIINP (Novus Biologicals, Wiesbaden Nordenstadt, Germany, Catalog 131 No. NBP2-76434; sensitivity 14.06 pg/ml) and EGF (Quantikine ELISA, R&D system; 132 Minneapolis, MN, USA, Catalog No. DEG00; sensitivity 0.7 pg/ml) were measured using 133 enzyme-linked immunosorbent assay (ELISA), according to manufacturer’s protocols. ..



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    Image Search Results


    ECH alleviates cisplatin-induced renal injury and inflammation in vivo. ( A ) Serum BUN levels in each group. ( B ) Serum creatinine levels of each group. ( C ) Serum KIM-1 levels detected by ELISA. ( D ) Serum NGAL levels detected by ELISA. ( E ) Representative H&E, PAS and KIM-1 immunohistochemical staining images of kidney tissues and quantitative analysis. ( F ) Western blot analysis and quantitative analysis of KIM-1 and NGAL protein in kidney tissues. ( G – J ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in kidney tissues. ** p < 0.01, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01 vs. cisplatin group. n = 3 mice per group.

    Journal: Biomedicines

    Article Title: Echinacoside Alleviates Cisplatin-Induced Acute Kidney Injury by Regulating Bap1 to Inhibit Ferroptosis

    doi: 10.3390/biomedicines14092127

    Figure Lengend Snippet: ECH alleviates cisplatin-induced renal injury and inflammation in vivo. ( A ) Serum BUN levels in each group. ( B ) Serum creatinine levels of each group. ( C ) Serum KIM-1 levels detected by ELISA. ( D ) Serum NGAL levels detected by ELISA. ( E ) Representative H&E, PAS and KIM-1 immunohistochemical staining images of kidney tissues and quantitative analysis. ( F ) Western blot analysis and quantitative analysis of KIM-1 and NGAL protein in kidney tissues. ( G – J ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in kidney tissues. ** p < 0.01, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01 vs. cisplatin group. n = 3 mice per group.

    Article Snippet: After membrane blocking, primary antibodies targeting KIM-1 (A24493, ABclonal, Wuhan, China), NGAL (A2092, ABclonal, Wuhan, China), GPX4 (A27995, ABclonal, Wuhan, China), Slc7a11 (A25302, ABclonal, Wuhan, China), Bap1 (A6533SP, ABclonal, Wuhan, China) and the internal reference GAPDH (A19056, ABclonal, Wuhan, China) were incubated overnight at 4 °C, followed by incubation with corresponding secondary antibodies.

    Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Analysis, Western Blot, Control

    ECH relieves cisplatin-induced cell damage in vitro. ( A ) HK-2 cell viability treated after treatment with different concentrations of ECH. ( B ) HK-2 cell viability under cisplatin stimulation with gradient ECH intervention. ( C ) Representative PI staining images of HK-2 cells and quantitative analysis. ( D ) Western blot and quantitative analysis of KIM-1 and NGAL proteins in HK-2 cells. ( E – H ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in HK-2 cells. ns, no significancant; * p < 0.05, ** p < 0.01 vs. control group; # p < 0.05 vs. cisplatin group.

    Journal: Biomedicines

    Article Title: Echinacoside Alleviates Cisplatin-Induced Acute Kidney Injury by Regulating Bap1 to Inhibit Ferroptosis

    doi: 10.3390/biomedicines14092127

    Figure Lengend Snippet: ECH relieves cisplatin-induced cell damage in vitro. ( A ) HK-2 cell viability treated after treatment with different concentrations of ECH. ( B ) HK-2 cell viability under cisplatin stimulation with gradient ECH intervention. ( C ) Representative PI staining images of HK-2 cells and quantitative analysis. ( D ) Western blot and quantitative analysis of KIM-1 and NGAL proteins in HK-2 cells. ( E – H ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in HK-2 cells. ns, no significancant; * p < 0.05, ** p < 0.01 vs. control group; # p < 0.05 vs. cisplatin group.

    Article Snippet: After membrane blocking, primary antibodies targeting KIM-1 (A24493, ABclonal, Wuhan, China), NGAL (A2092, ABclonal, Wuhan, China), GPX4 (A27995, ABclonal, Wuhan, China), Slc7a11 (A25302, ABclonal, Wuhan, China), Bap1 (A6533SP, ABclonal, Wuhan, China) and the internal reference GAPDH (A19056, ABclonal, Wuhan, China) were incubated overnight at 4 °C, followed by incubation with corresponding secondary antibodies.

    Techniques: In Vitro, Staining, Analysis, Western Blot, Control

    The effects of WRT on renal morphology changes in db/db mice . (A) Kidney type and (B) Right/left kidney weight change. (C) Representative periodic acid-Schiff (PAS) staining of kidney section (5 mm, Magnification: 40×, scale bars:100 μm) and (D) Glomerular surface area and mesangial surface area. (E) Urinary KIM-1 measurement for indication of tubular injury. db/m, non-diabetic control mice; db/db, diabetic mice (untreated); vilda, vildagliptin-treated mice (positive control; db/db + vildagliptin); WRT, Wiryeongtang-treated mice (db/db + WRT). Data are expressed as mean±SD (n=5). **p<0.01 vs db/m group; #p<0.05, ##p<0.01 vs db/db group.

    Journal: Integrative Medicine Research

    Article Title: Ameliorative effects of Wiryeongtang (herbal medicine) on cardiorenal complications via modulation of inflammatory and fibrotic responses in diabetic mice

    doi: 10.1016/j.imr.2026.101322

    Figure Lengend Snippet: The effects of WRT on renal morphology changes in db/db mice . (A) Kidney type and (B) Right/left kidney weight change. (C) Representative periodic acid-Schiff (PAS) staining of kidney section (5 mm, Magnification: 40×, scale bars:100 μm) and (D) Glomerular surface area and mesangial surface area. (E) Urinary KIM-1 measurement for indication of tubular injury. db/m, non-diabetic control mice; db/db, diabetic mice (untreated); vilda, vildagliptin-treated mice (positive control; db/db + vildagliptin); WRT, Wiryeongtang-treated mice (db/db + WRT). Data are expressed as mean±SD (n=5). **p<0.01 vs db/m group; #p<0.05, ##p<0.01 vs db/db group.

    Article Snippet: Serum insulin (Shibyagi Co., Gunma, Japan) and KIM-1 (LSBIO., Ltd., Ishihara Shibukawa, Gunma, Japan) levels were measured using ELISA kits.

    Techniques: Staining, Control, Positive Control