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human kim  (Boster Bio)


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    Structured Review

    Boster Bio human kim
    Human Kim, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kim+1/Human+Lipocalin-2%2FNGAL+ELISA+Kit+PicoKine/10__1016_slash_j__aicoj__2026__100167-99-1-5
    Average 92 stars, based on 23 article reviews
    human kim - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    other:

    Article Title: The Herbal Constituents in An-Gong-Niu-Huang Wan (AGNH) Protect against Cinnabar- and Realgar-Induced Hepatorenal Toxicity and Accumulations of Mercury and Arsenic in Mice
    Article Snippet: Specific antigens for the primary antibodies were as follows: GST α 1, GSTmu, OCT1, OCT2, MRP1, aquaporin-9 (AQP-9), and β -actin (Bioworld Technology Co, Ltd., MN, USA); Na + -K + -ATPase, and MT-1 (Santa Cruz, CA, USA); KIM-1, GSTpi, MRP2, MRP4, P-gp, and OAT1 (Boster Biological Technology Co, Ltd., Hubei, China).

    Article Title: Elevated Urinary VEGF-A, Transferrin, and Angiotensinogen Levels in Normoalbuminuric Children and Adolescents with Type 1 Diabetes: Can They Be Early Markers of Diabetic Kidney Disease?
    Article Snippet: Objective: We hypothesized that diabetic kidney disease (DKD) begins early, before albuminuria occurs.. We therefore aimed to assess potential early urinary biomarkers of DKD in normoalbuminuric and normotensive children and adolescents with type 1 diabetes (T1D) to evaluate the relationship between these markers and clinical and laboratory risk factors for DKD.. Methods: This cross-sectional study included 75 children and adolescents with T1D (62% females, mean age 13.9 ± 3.2 years) with normoalbuminuria (an albumin/ creatinine ratio [ACR] below 30 mg/g creatinine).

    Blocking Assay:

    Article Title: Inhibition of TMEM16A improves cisplatin-induced acute kidney injury via preventing DRP1-mediated mitochondrial fission.
    Article Snippet: Acute kidney injury (AKI) is associated with high morbidity and mortality.. Our previous study has demonstrated that TMEM16A, a Ca-activated chloride channel, contributes to renal fibrosis progression in chronic kidney disease.. However, whether TMEM16A is involved in AKI is still unknown.

    Incubation:

    Article Title: Inhibition of TMEM16A improves cisplatin-induced acute kidney injury via preventing DRP1-mediated mitochondrial fission.
    Article Snippet: Acute kidney injury (AKI) is associated with high morbidity and mortality.. Our previous study has demonstrated that TMEM16A, a Ca-activated chloride channel, contributes to renal fibrosis progression in chronic kidney disease.. However, whether TMEM16A is involved in AKI is still unknown.

    Article Title: Vegfa promoter gene hypermethylation at HIF1α binding site is an early contributor to CKD progression after renal ischemia
    Article Snippet: .. The membranes were incubated with mouse anti-HSP72 antibody (ENZO Life Sciences, Cat. No. ADI-SPA-819F, 1:5000 dilution) or KIM-1 (Boster, Cat. No. PA1632, 1:5000) overnight at 4 °C. .. Thereafter; membranes were incubated with a secondary antibody, HRP-conjugated goat anti-mouse IgG, or anti-rabbit IgG, respectively (1:5000, Cat. No. sc-2031 or sc-2004, respectively, Santa Cruz).

    Article Title: Kidney Damage Following a 90-day Subchronic Inhalation Exposure to HTP Aerosol and Cigarette Smoke in Rats
    Article Snippet: .. Immunohistochemistry The para nized sections of kidney were prepared and incubated with primary antibodies including IL-18 (1:250, Boster, China), KIM-1 (1:200, Boster, China), and NGAL (1:200, Boster, China), secondary antibodies (Abcam, UK) and 3,3'-diaminobenzidine (DAB) chromogenic solution, respectively. .. At last, all the sections were sealed with neutral resin (Solarbio, China) and observed with the microscope (Olympus, Japan).

    Pyrolysis Gas Chromatography:

    Article Title: A new LKB1 activator, piericidin analogue S14, retards renal fibrosis through promoting autophagy and mitochondrial homeostasis in renal tubular epithelial cells
    Article Snippet: .. The primary antibodies used were as follows: Fibronectin (Sigma‐Aldrich, F3648), α‐SMA (Abcam, ab5694), Collagen I (Boster Biological Technology, BA0325), KIM-1 (Boster Biological Technology, BA3537), Klotho (R&D Systems, AF1819), PGC‐1α (Abcam, ab54481), TFAM (Boster Biological Technology, PB0413), TOMM20 (Abcam, ab186735), LC3B (Abcam, ab48394), p62/SQSTM1 (Abcam, ab109012), ATG5 (Boster Biological Technology, BA3525-2), mTOR (Abcam , ab2732), p-mTOR (Abcam, ab109268), STRAD (Abcam, ab192879), CAB39/M025(Abcam, ab51132), p-AMPKα (Cell Signaling Technology, 2535S), AMPKα (Cell Signaling Technology, 2532S), p-LKB1 (Cell Signaling Technology, 3482S), LKB1 (Santa Cruz Biotechnology, sc-32245), active-β-catenin (Cell Signaling Technology, 19807S), p16 INK4A (Abcam, ab189034), p14 ARF (Abcam, ab185620), PAI-1 (R&D Systems, AF1819), p-MARK(Cell Signaling Technology, 2532S), MARK2(Cell Signaling Technology, 9118S), p-SIK(Abmart, TA8189S), SIK(Proteintech,51045-1-AP), PP2C-α (Cell Signaling Technology, 3549S), GAPDH (Ray Antibody Biotech, RM2002), α‐tubulin (Ray Antibody Biotech, RM2007), and β-actin (Ray Antibody Biotech, RM2001). qRT-PCR Total RNA was obtained using a TRIzol RNA isolation system (Life Technologies, Grand Island, NY). .. Real-time PCR was performed on an ABI PRISM 7000 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Quantitative RT-PCR:

    Article Title: A new LKB1 activator, piericidin analogue S14, retards renal fibrosis through promoting autophagy and mitochondrial homeostasis in renal tubular epithelial cells
    Article Snippet: .. The primary antibodies used were as follows: Fibronectin (Sigma‐Aldrich, F3648), α‐SMA (Abcam, ab5694), Collagen I (Boster Biological Technology, BA0325), KIM-1 (Boster Biological Technology, BA3537), Klotho (R&D Systems, AF1819), PGC‐1α (Abcam, ab54481), TFAM (Boster Biological Technology, PB0413), TOMM20 (Abcam, ab186735), LC3B (Abcam, ab48394), p62/SQSTM1 (Abcam, ab109012), ATG5 (Boster Biological Technology, BA3525-2), mTOR (Abcam , ab2732), p-mTOR (Abcam, ab109268), STRAD (Abcam, ab192879), CAB39/M025(Abcam, ab51132), p-AMPKα (Cell Signaling Technology, 2535S), AMPKα (Cell Signaling Technology, 2532S), p-LKB1 (Cell Signaling Technology, 3482S), LKB1 (Santa Cruz Biotechnology, sc-32245), active-β-catenin (Cell Signaling Technology, 19807S), p16 INK4A (Abcam, ab189034), p14 ARF (Abcam, ab185620), PAI-1 (R&D Systems, AF1819), p-MARK(Cell Signaling Technology, 2532S), MARK2(Cell Signaling Technology, 9118S), p-SIK(Abmart, TA8189S), SIK(Proteintech,51045-1-AP), PP2C-α (Cell Signaling Technology, 3549S), GAPDH (Ray Antibody Biotech, RM2002), α‐tubulin (Ray Antibody Biotech, RM2007), and β-actin (Ray Antibody Biotech, RM2001). qRT-PCR Total RNA was obtained using a TRIzol RNA isolation system (Life Technologies, Grand Island, NY). .. Real-time PCR was performed on an ABI PRISM 7000 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Isolation:

    Article Title: A new LKB1 activator, piericidin analogue S14, retards renal fibrosis through promoting autophagy and mitochondrial homeostasis in renal tubular epithelial cells
    Article Snippet: .. The primary antibodies used were as follows: Fibronectin (Sigma‐Aldrich, F3648), α‐SMA (Abcam, ab5694), Collagen I (Boster Biological Technology, BA0325), KIM-1 (Boster Biological Technology, BA3537), Klotho (R&D Systems, AF1819), PGC‐1α (Abcam, ab54481), TFAM (Boster Biological Technology, PB0413), TOMM20 (Abcam, ab186735), LC3B (Abcam, ab48394), p62/SQSTM1 (Abcam, ab109012), ATG5 (Boster Biological Technology, BA3525-2), mTOR (Abcam , ab2732), p-mTOR (Abcam, ab109268), STRAD (Abcam, ab192879), CAB39/M025(Abcam, ab51132), p-AMPKα (Cell Signaling Technology, 2535S), AMPKα (Cell Signaling Technology, 2532S), p-LKB1 (Cell Signaling Technology, 3482S), LKB1 (Santa Cruz Biotechnology, sc-32245), active-β-catenin (Cell Signaling Technology, 19807S), p16 INK4A (Abcam, ab189034), p14 ARF (Abcam, ab185620), PAI-1 (R&D Systems, AF1819), p-MARK(Cell Signaling Technology, 2532S), MARK2(Cell Signaling Technology, 9118S), p-SIK(Abmart, TA8189S), SIK(Proteintech,51045-1-AP), PP2C-α (Cell Signaling Technology, 3549S), GAPDH (Ray Antibody Biotech, RM2002), α‐tubulin (Ray Antibody Biotech, RM2007), and β-actin (Ray Antibody Biotech, RM2001). qRT-PCR Total RNA was obtained using a TRIzol RNA isolation system (Life Technologies, Grand Island, NY). .. Real-time PCR was performed on an ABI PRISM 7000 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Immunohistochemistry:

    Article Title: Kidney Damage Following a 90-day Subchronic Inhalation Exposure to HTP Aerosol and Cigarette Smoke in Rats
    Article Snippet: .. Immunohistochemistry The para nized sections of kidney were prepared and incubated with primary antibodies including IL-18 (1:250, Boster, China), KIM-1 (1:200, Boster, China), and NGAL (1:200, Boster, China), secondary antibodies (Abcam, UK) and 3,3'-diaminobenzidine (DAB) chromogenic solution, respectively. .. At last, all the sections were sealed with neutral resin (Solarbio, China) and observed with the microscope (Olympus, Japan).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Do old urinary biomarkers have a place in the new definition of hepatorenal syndrome in the Egyptian cirrhotic patients? A single-center experience
    Article Snippet: .. We measured urinary NGAL, KIM-1, by commercially available enzyme-linked immunosorbent assay (ELISA) kit (BOSTER Biological Technology Co. USA), according to the manufacturer’s instructions. ..



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    ECH alleviates cisplatin-induced renal injury and inflammation in vivo. ( A ) Serum BUN levels in each group. ( B ) Serum creatinine levels of each group. ( C ) <t>Serum</t> <t>KIM-1</t> levels detected by ELISA. ( D ) Serum NGAL levels detected by ELISA. ( E ) Representative H&E, PAS and KIM-1 immunohistochemical staining images of kidney tissues and quantitative analysis. ( F ) Western blot analysis and quantitative analysis of KIM-1 and NGAL protein in kidney tissues. ( G – J ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in kidney tissues. ** p < 0.01, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01 vs. cisplatin group. n = 3 mice per group.
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    ECH alleviates cisplatin-induced renal injury and inflammation in vivo. ( A ) Serum BUN levels in each group. ( B ) Serum creatinine levels of each group. ( C ) <t>Serum</t> <t>KIM-1</t> levels detected by ELISA. ( D ) Serum NGAL levels detected by ELISA. ( E ) Representative H&E, PAS and KIM-1 immunohistochemical staining images of kidney tissues and quantitative analysis. ( F ) Western blot analysis and quantitative analysis of KIM-1 and NGAL protein in kidney tissues. ( G – J ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in kidney tissues. ** p < 0.01, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01 vs. cisplatin group. n = 3 mice per group.
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    The effects of WRT on renal morphology changes in db/db mice . (A) Kidney type and (B) Right/left kidney weight change. (C) Representative periodic acid-Schiff (PAS) staining of kidney section (5 mm, Magnification: 40×, scale bars:100 μm) and (D) Glomerular surface area and mesangial surface area. (E) <t>Urinary</t> <t>KIM-1</t> measurement for indication of tubular injury. db/m, non-diabetic control mice; db/db, diabetic mice (untreated); vilda, vildagliptin-treated mice (positive control; db/db + vildagliptin); WRT, Wiryeongtang-treated mice (db/db + WRT). Data are expressed as mean±SD (n=5). **p<0.01 vs db/m group; #p<0.05, ##p<0.01 vs db/db group.
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    Image Search Results


    ECH alleviates cisplatin-induced renal injury and inflammation in vivo. ( A ) Serum BUN levels in each group. ( B ) Serum creatinine levels of each group. ( C ) Serum KIM-1 levels detected by ELISA. ( D ) Serum NGAL levels detected by ELISA. ( E ) Representative H&E, PAS and KIM-1 immunohistochemical staining images of kidney tissues and quantitative analysis. ( F ) Western blot analysis and quantitative analysis of KIM-1 and NGAL protein in kidney tissues. ( G – J ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in kidney tissues. ** p < 0.01, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01 vs. cisplatin group. n = 3 mice per group.

    Journal: Biomedicines

    Article Title: Echinacoside Alleviates Cisplatin-Induced Acute Kidney Injury by Regulating Bap1 to Inhibit Ferroptosis

    doi: 10.3390/biomedicines14092127

    Figure Lengend Snippet: ECH alleviates cisplatin-induced renal injury and inflammation in vivo. ( A ) Serum BUN levels in each group. ( B ) Serum creatinine levels of each group. ( C ) Serum KIM-1 levels detected by ELISA. ( D ) Serum NGAL levels detected by ELISA. ( E ) Representative H&E, PAS and KIM-1 immunohistochemical staining images of kidney tissues and quantitative analysis. ( F ) Western blot analysis and quantitative analysis of KIM-1 and NGAL protein in kidney tissues. ( G – J ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in kidney tissues. ** p < 0.01, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01 vs. cisplatin group. n = 3 mice per group.

    Article Snippet: After membrane blocking, primary antibodies targeting KIM-1 (A24493, ABclonal, Wuhan, China), NGAL (A2092, ABclonal, Wuhan, China), GPX4 (A27995, ABclonal, Wuhan, China), Slc7a11 (A25302, ABclonal, Wuhan, China), Bap1 (A6533SP, ABclonal, Wuhan, China) and the internal reference GAPDH (A19056, ABclonal, Wuhan, China) were incubated overnight at 4 °C, followed by incubation with corresponding secondary antibodies.

    Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Analysis, Western Blot, Control

    ECH relieves cisplatin-induced cell damage in vitro. ( A ) HK-2 cell viability treated after treatment with different concentrations of ECH. ( B ) HK-2 cell viability under cisplatin stimulation with gradient ECH intervention. ( C ) Representative PI staining images of HK-2 cells and quantitative analysis. ( D ) Western blot and quantitative analysis of KIM-1 and NGAL proteins in HK-2 cells. ( E – H ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in HK-2 cells. ns, no significancant; * p < 0.05, ** p < 0.01 vs. control group; # p < 0.05 vs. cisplatin group.

    Journal: Biomedicines

    Article Title: Echinacoside Alleviates Cisplatin-Induced Acute Kidney Injury by Regulating Bap1 to Inhibit Ferroptosis

    doi: 10.3390/biomedicines14092127

    Figure Lengend Snippet: ECH relieves cisplatin-induced cell damage in vitro. ( A ) HK-2 cell viability treated after treatment with different concentrations of ECH. ( B ) HK-2 cell viability under cisplatin stimulation with gradient ECH intervention. ( C ) Representative PI staining images of HK-2 cells and quantitative analysis. ( D ) Western blot and quantitative analysis of KIM-1 and NGAL proteins in HK-2 cells. ( E – H ) Relative mRNA levels of IL-1β, MCP-1, TNF-α and IL-6 in HK-2 cells. ns, no significancant; * p < 0.05, ** p < 0.01 vs. control group; # p < 0.05 vs. cisplatin group.

    Article Snippet: After membrane blocking, primary antibodies targeting KIM-1 (A24493, ABclonal, Wuhan, China), NGAL (A2092, ABclonal, Wuhan, China), GPX4 (A27995, ABclonal, Wuhan, China), Slc7a11 (A25302, ABclonal, Wuhan, China), Bap1 (A6533SP, ABclonal, Wuhan, China) and the internal reference GAPDH (A19056, ABclonal, Wuhan, China) were incubated overnight at 4 °C, followed by incubation with corresponding secondary antibodies.

    Techniques: In Vitro, Staining, Analysis, Western Blot, Control

    The effects of WRT on renal morphology changes in db/db mice . (A) Kidney type and (B) Right/left kidney weight change. (C) Representative periodic acid-Schiff (PAS) staining of kidney section (5 mm, Magnification: 40×, scale bars:100 μm) and (D) Glomerular surface area and mesangial surface area. (E) Urinary KIM-1 measurement for indication of tubular injury. db/m, non-diabetic control mice; db/db, diabetic mice (untreated); vilda, vildagliptin-treated mice (positive control; db/db + vildagliptin); WRT, Wiryeongtang-treated mice (db/db + WRT). Data are expressed as mean±SD (n=5). **p<0.01 vs db/m group; #p<0.05, ##p<0.01 vs db/db group.

    Journal: Integrative Medicine Research

    Article Title: Ameliorative effects of Wiryeongtang (herbal medicine) on cardiorenal complications via modulation of inflammatory and fibrotic responses in diabetic mice

    doi: 10.1016/j.imr.2026.101322

    Figure Lengend Snippet: The effects of WRT on renal morphology changes in db/db mice . (A) Kidney type and (B) Right/left kidney weight change. (C) Representative periodic acid-Schiff (PAS) staining of kidney section (5 mm, Magnification: 40×, scale bars:100 μm) and (D) Glomerular surface area and mesangial surface area. (E) Urinary KIM-1 measurement for indication of tubular injury. db/m, non-diabetic control mice; db/db, diabetic mice (untreated); vilda, vildagliptin-treated mice (positive control; db/db + vildagliptin); WRT, Wiryeongtang-treated mice (db/db + WRT). Data are expressed as mean±SD (n=5). **p<0.01 vs db/m group; #p<0.05, ##p<0.01 vs db/db group.

    Article Snippet: Serum insulin (Shibyagi Co., Gunma, Japan) and KIM-1 (LSBIO., Ltd., Ishihara Shibukawa, Gunma, Japan) levels were measured using ELISA kits.

    Techniques: Staining, Control, Positive Control