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opa1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc opa1
    A , gene expression of fission and fusion markers Fis1, Drp1, <t>OPA1</t> and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
    Opa1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 437 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/keap1/KEAP1+Rabbit+mAb/pmc13674193-101-20-21
    Average 96 stars, based on 437 article reviews
    opa1 - by Bioz Stars, 2026-11
    96/100 stars

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    1) Product Images from "Mitochondrial dynamics and repair differences in two mouse models of Friedreich's ataxia"

    Article Title: Mitochondrial dynamics and repair differences in two mouse models of Friedreich's ataxia

    Journal: The Journal of Physiology

    doi: 10.1113/JP291356

    A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
    Figure Legend Snippet: A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

    Techniques Used: Gene Expression, Western Blot, Expressing

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    Article Snippet: Subsequently, samples were separated by 12% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) using molecular weight markers (Western blots used different protein markers depending on availability: 8–180 kDa, Yeasen, #20350ES72, or 10–180 kDa from GeneTech, #R1001‐002), and then transferred onto 0.45 μ m polyvinylidene difluoride (PVDF) membranes. .. The membranes were blocked with 5% skim milk in TBST buffer for 2 h or Protein Free Rapid Blocking Buffer (Epizyme, #PS108P) for 30 min at room temperature, then incubated overnight at 4°C with primary antibodies diluted in dilution solution (Beyotime, #P0023A): GAPDH (at a dilution of 1:20000, Proteintech, #60004‐1‐Ig), Tubulin (at a dilution of 1:2000, Abmart, # M30109 ), Ezrin (at a dilution of 1:2000, ZenBio, # R24261 ), Radixin (at a dilution of 1:2000, Selleck, #A5280/#F1012), Moesin (at a dilution of 1:2000, Selleck, #A5744), and p‐Ezrin/Radixin/Moesin (Thr567/Thr564/Thr558) (at a dilution of 1:2000, Antigen sequence: GRDKYKpTLRQIR, p stands for phosphorylation, ZenBio, # R26293 ), HMOX1 (at a dilution of 1:1000, ZenBio, #670853), KEAP1 (at a dilution of 1:2000, Cell Signaling Technology, #8047), NRF2 (at a dilution of 1:2000, ZenBio, #R380773; Abcam, #ab31163), INF2 (at a dilution of 1:4000, Proteintech, #20466‐1‐AP), Lamin B1 (at a dilution of 1:2000, Cell Signaling Technology, #13435). .. Corresponding secondary antibodies (at a dilution of 1:5000, CST, #7076S, #7074S) were diluted in TBST and incubated for 3 h at 4°C.

    Article Title: ERM Inhibition Confers Ferroptosis Resistance through ROS-Induced NRF2 Signaling.
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    Article Title: METTL3-driven m 6 A modification of NLRC5 promotes renal fibrosis in chronic kidney disease through Keap1/Nrf2/ARE signaling pathway
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    Incubation:

    Article Title: ERM Inhibition Confers Ferroptosis Resistance through ROS‐Induced NRF2 Signaling
    Article Snippet: Subsequently, samples were separated by 12% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) using molecular weight markers (Western blots used different protein markers depending on availability: 8–180 kDa, Yeasen, #20350ES72, or 10–180 kDa from GeneTech, #R1001‐002), and then transferred onto 0.45 μ m polyvinylidene difluoride (PVDF) membranes. .. The membranes were blocked with 5% skim milk in TBST buffer for 2 h or Protein Free Rapid Blocking Buffer (Epizyme, #PS108P) for 30 min at room temperature, then incubated overnight at 4°C with primary antibodies diluted in dilution solution (Beyotime, #P0023A): GAPDH (at a dilution of 1:20000, Proteintech, #60004‐1‐Ig), Tubulin (at a dilution of 1:2000, Abmart, # M30109 ), Ezrin (at a dilution of 1:2000, ZenBio, # R24261 ), Radixin (at a dilution of 1:2000, Selleck, #A5280/#F1012), Moesin (at a dilution of 1:2000, Selleck, #A5744), and p‐Ezrin/Radixin/Moesin (Thr567/Thr564/Thr558) (at a dilution of 1:2000, Antigen sequence: GRDKYKpTLRQIR, p stands for phosphorylation, ZenBio, # R26293 ), HMOX1 (at a dilution of 1:1000, ZenBio, #670853), KEAP1 (at a dilution of 1:2000, Cell Signaling Technology, #8047), NRF2 (at a dilution of 1:2000, ZenBio, #R380773; Abcam, #ab31163), INF2 (at a dilution of 1:4000, Proteintech, #20466‐1‐AP), Lamin B1 (at a dilution of 1:2000, Cell Signaling Technology, #13435). .. Corresponding secondary antibodies (at a dilution of 1:5000, CST, #7076S, #7074S) were diluted in TBST and incubated for 3 h at 4°C.

    Article Title: ERM Inhibition Confers Ferroptosis Resistance through ROS-Induced NRF2 Signaling.
    Article Snippet: Subsequently, samples were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using molecular weight markers (Western blots used different protein markers depending on availability: 8–180 kDa, Yeasen, #20350ES72, or 10–180 kDa from GeneTech, #R1001-002), and then transferred onto 0.45 μm polyvinylidene difluoride (PVDF) membranes. .. The membranes were blocked with 5% skim milk in TBST buffer for 2 h or Protein Free Rapid Blocking Buffer (Epizyme, #PS108P) for 30 min at room temperature, then incubated overnight at 4◦C with primary antibodies diluted in dilution solution (Beyotime, #P0023A): GAPDH (at a dilution of 1:20000, Proteintech, #60004-1-Ig), Tubulin (at a dilution of 1:2000, Abmart, #M30109), Ezrin (at a dilution of 1:2000, ZenBio, #R24261), Radixin (at a dilution of 1:2000, Selleck, #A5280/#F1012), Moesin (at a dilution of 1:2000, Selleck, #A5744), and p-Ezrin/Radixin/Moesin (Thr567/Thr564/Thr558) (at a dilution of 1:2000, Antigen sequence: GRDKYKpTLRQIR, p stands for phosphorylation, ZenBio, #R26293), HMOX1 (at a dilution of 1:1000, ZenBio, #670853), KEAP1 (at a dilution of 1:2000, Cell Signaling Technology, #8047), NRF2 (at a dilution of 1:2000, ZenBio, #R380773; Abcam, #ab31163), INF2 (at a dilution of 1:4000, Proteintech, #20466-1-AP), Lamin B1 (at a dilution of 1:2000, Cell Signaling Technology, #13435). .. Corresponding secondary antibodies (at a dilution of 1:5000, CST, #7076S, #7074S) were diluted in TBST and incubated for 3 h at 4◦C.

    Article Title: Unveiling a Novel Mechanism in Noise-Induced Hearing Loss: Oxeiptosis-Mediated Regulated Cell Death of Cochlear Hair Cell.
    Article Snippet: .. Then, they were incubated with KEAP1 (8047, Cell Signalling Technology) and PGAM5 rabbit (HPA036978, Thermo Fisher Scientific). .. The samples were washed with PBS-T for 5 min three times before being incubated with secondary antibodies at room temperature for 1 h. The secondary antibodies included the Alexa Fluor 647-conjugated donkey anti-rabbit or mouse IgG (A-31573, A-31571, Invitrogen, California, USA) and the Alexa Fluor 488-conjugated donkey anti-rabbit or mouse IgG (A-21206, R37114, Invitrogen).

    Article Title: Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signaling
    Article Snippet: Equal amounts of protein (30-50 μg) were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, IPVH00010). .. Membranes were blocked with 5% non-fat milk in TBST for 1 h and incubated overnight at 4 °C with primary antibodies: SIRT6 (12486, Cell Signaling Technology), NRF2 (ab62352, Abcam), HO-1 (ab68477, Abcam), NQO1 (ab80588, Abcam), KEAP1 (8047, Cell Signaling Technology), NLRP3 (AG-20B-0014, AdipoGen), cleaved caspase-1 (4199, Cell Signaling Technology), cleaved IL-1β (83186, Cell Signaling Technology), and β-actin (A1978, Sigma-Aldrich). .. After incubation with appropriate HRP-conjugated secondary antibodies (Cell Signaling Technology), bands were visualized using enhanced chemiluminescence (ECL) detection reagent (Pierce, 32109) and quantified using ImageJ software.

    Article Title: METTL3-driven m 6 A modification of NLRC5 promotes renal fibrosis in chronic kidney disease through Keap1/Nrf2/ARE signaling pathway
    Article Snippet: Equal amounts (30 μg) of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad, Hercules, CA, USA). .. After blocking with 5% non-fat milk in TBST for 2 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies targeting α-SMA (1:1000, #19245), Collagen I (1:1000, #72026), E-cadherin (1:1000, #3195), Nrf2 (1:1000, #12721), Keap1 (1:1000, #8047), HO-1 (1:1000, #70081), NQO1 (1:1000, #62262), METTL3 (1:1000, #86132), NLRC5 (1:1000, #72379), Lamin B1 (1:1000, #13435), Lamin B1 (1:1000, #13435) and GAPDH (1:5000, #5174) (all from Cell Signaling Technology unless otherwise stated; dilution 1:1000, GAPDH 1:5000). .. After washing with TBST, membranes were incubated with HRP-conjugated secondary antibodies (Beyotime Biotechnology) for 2 h at room temperature.

    Article Title: Unveiling a Novel Mechanism in Noise-Induced Hearing Loss: Oxeiptosis-Mediated Regulated Cell Death of Cochlear Hair Cell.
    Article Snippet: .. Primary antibody incubation was carried out overnight at 4 °C using KEAP1 (8047, Cell Signalling Technology, Massachusetts, USA) and PGAM5 rabbit (HPA036978, Thermo Fisher Scientific) monoclonal antibodies. .. Following another PBS washing cycle, cells were exposed to speciesmatched fluorescent secondary antibodies (SA00001-2, Proteintech) for 1 h at ambient temperature.

    Sequencing:

    Article Title: ERM Inhibition Confers Ferroptosis Resistance through ROS‐Induced NRF2 Signaling
    Article Snippet: Subsequently, samples were separated by 12% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) using molecular weight markers (Western blots used different protein markers depending on availability: 8–180 kDa, Yeasen, #20350ES72, or 10–180 kDa from GeneTech, #R1001‐002), and then transferred onto 0.45 μ m polyvinylidene difluoride (PVDF) membranes. .. The membranes were blocked with 5% skim milk in TBST buffer for 2 h or Protein Free Rapid Blocking Buffer (Epizyme, #PS108P) for 30 min at room temperature, then incubated overnight at 4°C with primary antibodies diluted in dilution solution (Beyotime, #P0023A): GAPDH (at a dilution of 1:20000, Proteintech, #60004‐1‐Ig), Tubulin (at a dilution of 1:2000, Abmart, # M30109 ), Ezrin (at a dilution of 1:2000, ZenBio, # R24261 ), Radixin (at a dilution of 1:2000, Selleck, #A5280/#F1012), Moesin (at a dilution of 1:2000, Selleck, #A5744), and p‐Ezrin/Radixin/Moesin (Thr567/Thr564/Thr558) (at a dilution of 1:2000, Antigen sequence: GRDKYKpTLRQIR, p stands for phosphorylation, ZenBio, # R26293 ), HMOX1 (at a dilution of 1:1000, ZenBio, #670853), KEAP1 (at a dilution of 1:2000, Cell Signaling Technology, #8047), NRF2 (at a dilution of 1:2000, ZenBio, #R380773; Abcam, #ab31163), INF2 (at a dilution of 1:4000, Proteintech, #20466‐1‐AP), Lamin B1 (at a dilution of 1:2000, Cell Signaling Technology, #13435). .. Corresponding secondary antibodies (at a dilution of 1:5000, CST, #7076S, #7074S) were diluted in TBST and incubated for 3 h at 4°C.

    Article Title: ERM Inhibition Confers Ferroptosis Resistance through ROS-Induced NRF2 Signaling.
    Article Snippet: Subsequently, samples were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using molecular weight markers (Western blots used different protein markers depending on availability: 8–180 kDa, Yeasen, #20350ES72, or 10–180 kDa from GeneTech, #R1001-002), and then transferred onto 0.45 μm polyvinylidene difluoride (PVDF) membranes. .. The membranes were blocked with 5% skim milk in TBST buffer for 2 h or Protein Free Rapid Blocking Buffer (Epizyme, #PS108P) for 30 min at room temperature, then incubated overnight at 4◦C with primary antibodies diluted in dilution solution (Beyotime, #P0023A): GAPDH (at a dilution of 1:20000, Proteintech, #60004-1-Ig), Tubulin (at a dilution of 1:2000, Abmart, #M30109), Ezrin (at a dilution of 1:2000, ZenBio, #R24261), Radixin (at a dilution of 1:2000, Selleck, #A5280/#F1012), Moesin (at a dilution of 1:2000, Selleck, #A5744), and p-Ezrin/Radixin/Moesin (Thr567/Thr564/Thr558) (at a dilution of 1:2000, Antigen sequence: GRDKYKpTLRQIR, p stands for phosphorylation, ZenBio, #R26293), HMOX1 (at a dilution of 1:1000, ZenBio, #670853), KEAP1 (at a dilution of 1:2000, Cell Signaling Technology, #8047), NRF2 (at a dilution of 1:2000, ZenBio, #R380773; Abcam, #ab31163), INF2 (at a dilution of 1:4000, Proteintech, #20466-1-AP), Lamin B1 (at a dilution of 1:2000, Cell Signaling Technology, #13435). .. Corresponding secondary antibodies (at a dilution of 1:5000, CST, #7076S, #7074S) were diluted in TBST and incubated for 3 h at 4◦C.

    Phospho-proteomics:

    Article Title: ERM Inhibition Confers Ferroptosis Resistance through ROS‐Induced NRF2 Signaling
    Article Snippet: Subsequently, samples were separated by 12% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) using molecular weight markers (Western blots used different protein markers depending on availability: 8–180 kDa, Yeasen, #20350ES72, or 10–180 kDa from GeneTech, #R1001‐002), and then transferred onto 0.45 μ m polyvinylidene difluoride (PVDF) membranes. .. The membranes were blocked with 5% skim milk in TBST buffer for 2 h or Protein Free Rapid Blocking Buffer (Epizyme, #PS108P) for 30 min at room temperature, then incubated overnight at 4°C with primary antibodies diluted in dilution solution (Beyotime, #P0023A): GAPDH (at a dilution of 1:20000, Proteintech, #60004‐1‐Ig), Tubulin (at a dilution of 1:2000, Abmart, # M30109 ), Ezrin (at a dilution of 1:2000, ZenBio, # R24261 ), Radixin (at a dilution of 1:2000, Selleck, #A5280/#F1012), Moesin (at a dilution of 1:2000, Selleck, #A5744), and p‐Ezrin/Radixin/Moesin (Thr567/Thr564/Thr558) (at a dilution of 1:2000, Antigen sequence: GRDKYKpTLRQIR, p stands for phosphorylation, ZenBio, # R26293 ), HMOX1 (at a dilution of 1:1000, ZenBio, #670853), KEAP1 (at a dilution of 1:2000, Cell Signaling Technology, #8047), NRF2 (at a dilution of 1:2000, ZenBio, #R380773; Abcam, #ab31163), INF2 (at a dilution of 1:4000, Proteintech, #20466‐1‐AP), Lamin B1 (at a dilution of 1:2000, Cell Signaling Technology, #13435). .. Corresponding secondary antibodies (at a dilution of 1:5000, CST, #7076S, #7074S) were diluted in TBST and incubated for 3 h at 4°C.

    Article Title: ERM Inhibition Confers Ferroptosis Resistance through ROS-Induced NRF2 Signaling.
    Article Snippet: Subsequently, samples were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using molecular weight markers (Western blots used different protein markers depending on availability: 8–180 kDa, Yeasen, #20350ES72, or 10–180 kDa from GeneTech, #R1001-002), and then transferred onto 0.45 μm polyvinylidene difluoride (PVDF) membranes. .. The membranes were blocked with 5% skim milk in TBST buffer for 2 h or Protein Free Rapid Blocking Buffer (Epizyme, #PS108P) for 30 min at room temperature, then incubated overnight at 4◦C with primary antibodies diluted in dilution solution (Beyotime, #P0023A): GAPDH (at a dilution of 1:20000, Proteintech, #60004-1-Ig), Tubulin (at a dilution of 1:2000, Abmart, #M30109), Ezrin (at a dilution of 1:2000, ZenBio, #R24261), Radixin (at a dilution of 1:2000, Selleck, #A5280/#F1012), Moesin (at a dilution of 1:2000, Selleck, #A5744), and p-Ezrin/Radixin/Moesin (Thr567/Thr564/Thr558) (at a dilution of 1:2000, Antigen sequence: GRDKYKpTLRQIR, p stands for phosphorylation, ZenBio, #R26293), HMOX1 (at a dilution of 1:1000, ZenBio, #670853), KEAP1 (at a dilution of 1:2000, Cell Signaling Technology, #8047), NRF2 (at a dilution of 1:2000, ZenBio, #R380773; Abcam, #ab31163), INF2 (at a dilution of 1:4000, Proteintech, #20466-1-AP), Lamin B1 (at a dilution of 1:2000, Cell Signaling Technology, #13435). .. Corresponding secondary antibodies (at a dilution of 1:5000, CST, #7076S, #7074S) were diluted in TBST and incubated for 3 h at 4◦C.

    Cell-Signaling:

    Article Title: Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signaling
    Article Snippet: Equal amounts of protein (30-50 μg) were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, IPVH00010). .. Membranes were blocked with 5% non-fat milk in TBST for 1 h and incubated overnight at 4 °C with primary antibodies: SIRT6 (12486, Cell Signaling Technology), NRF2 (ab62352, Abcam), HO-1 (ab68477, Abcam), NQO1 (ab80588, Abcam), KEAP1 (8047, Cell Signaling Technology), NLRP3 (AG-20B-0014, AdipoGen), cleaved caspase-1 (4199, Cell Signaling Technology), cleaved IL-1β (83186, Cell Signaling Technology), and β-actin (A1978, Sigma-Aldrich). .. After incubation with appropriate HRP-conjugated secondary antibodies (Cell Signaling Technology), bands were visualized using enhanced chemiluminescence (ECL) detection reagent (Pierce, 32109) and quantified using ImageJ software.

    Bioprocessing:

    Article Title: Unveiling a Novel Mechanism in Noise-Induced Hearing Loss: Oxeiptosis-Mediated Regulated Cell Death of Cochlear Hair Cell.
    Article Snippet: .. Primary antibody incubation was carried out overnight at 4 °C using KEAP1 (8047, Cell Signalling Technology, Massachusetts, USA) and PGAM5 rabbit (HPA036978, Thermo Fisher Scientific) monoclonal antibodies. .. Following another PBS washing cycle, cells were exposed to speciesmatched fluorescent secondary antibodies (SA00001-2, Proteintech) for 1 h at ambient temperature.



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    Proteintech keap 1
    A , gene expression of fission and fusion markers Fis1, Drp1, <t>OPA1</t> and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
    Keap 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    keap 1  (ABclonal Biotechnology)
    97
    ABclonal Biotechnology keap 1
    A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).
    Keap 1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/keap1/KEAP1+Rabbit+pAb/bio_rxiv__64898__2026__09__29__753356-35-2-17
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    keap 1 - by Bioz Stars, 2026-11
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    keap1  (ABclonal Biotechnology)
    94
    ABclonal Biotechnology keap1
    A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).
    Keap1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/keap1/ALDH1A1+Rabbit+pAb/pm42833464-87-39-41
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    keap1  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology keap1
    A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).
    Keap1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

    Journal: The Journal of Physiology

    Article Title: Mitochondrial dynamics and repair differences in two mouse models of Friedreich's ataxia

    doi: 10.1113/JP291356

    Figure Lengend Snippet: A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

    Article Snippet: Primary antibodies: TFAM (Cell Signalling Technology, 8076), DRP1 (Cell Signalling Technology, 8570), FIS1 (Cell Signalling Technology, 32525), MFN2 (Abcam, ab56889), OPA1 (Cell Signalling Technology, 8047), Pink1 (Abcam, 23707), Parkin (Cell Signalling Technology, 32833), p62 (Abcam, ab56416), FUNDC1 (Cell Signalling Technology, 49240), BNIP3L (Cell Signalling Technology, 12396), LC3 (Cell Signalling Technology, 12 741), ATG7 (Cell Signalling Technology, 8558), ATG12–5 (Cell Signalling Technology, 4180), LAMP1 (Abcam, 24170), CD63 (Cell Signalling Technology, 10112), Cathepsin D (Cell Signalling, 74089), GAPDH (Abcam, ab8245), αTubulin (Millipore Sigma, T6199).

    Techniques: Gene Expression, Western Blot, Expressing

    A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).

    Journal: bioRxiv

    Article Title: Combined celecoxib and NDGA induces a non-canonical, autophagy-independent redox cell death via p62/KEAP1 in colorectal cancer

    doi: 10.64898/2026.09.29.753356

    Figure Lengend Snippet: A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).

    Article Snippet: NRF-2 (A3577), KEAP-1 (A25951), SQSTM1/p62 (A19700), Phospho-SQSTM1/p62-S349 (A20737), and ALOX5 (A2877) phosphor-ULK1 S555 (AP1495) were purchased from ABclonal (Woburn, MA, USA).

    Techniques: Western Blot, Analysis, Control