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recombinant protein standards  (R&D Systems)


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    R&D Systems recombinant protein standards
    Recombinant Protein Standards, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kc+standard/Mouse+CXCL1%2FGRO+alpha%2FKC%2FCINC-1+Antibody/pm37499656-306-20-27
    Average 94 stars, based on 47 article reviews
    recombinant protein standards - by Bioz Stars, 2026-10
    94/100 stars

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    Concentration Assay:

    Article Title: Sputum mucin 1 is increased during the acute phase of chronic obstructive pulmonary disease exacerbation
    Article Snippet: Finally, extravidin peroxidase (1:1,000 in PBS, Cat. E2886, Sigma) and TMB (tetramethylbenzidine) substrate reagent set (Cat. 555214, BD Biosciences Pharmingen) were used for detection. .. The concentration of KC was determined from a standard curve by using a KC standard (453-KC, R&D system). .. Lung tissues were homogenized in RIPA buffer (20 mM Tris, pH 7.4, 150 mM NaCl, 1% Triton X-100, 1% Na deoxycholate, 2 mM EGTA, 2 mM EDTA, 0.1% SDS) containing protease inhibitor cocktail (1:100, Sigma).



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    Image Search Results


    Rg1 reduced NETs in lung tissues. (A) Representative immunofluorescence images and quantification of H3cit and NE in the lungs (n = 3; scale bar: 50 μm). (B) Representative western blot images of H3cit and NE levels in the lungs and quantitative data ( n = 3). GAPDH was used as a loading control. (C-E) Serum levels of CXCL1, CXCL2, and G-CSF were detected via ELISA ( n = 10). (F-H) The mRNA expression levels of CXCL1, CXCL2 and G-CSF in lung tissues were determined by qRT–PCR ( n = 3). All the data are presented as mean ± SEM. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. the model group.

    Journal: Journal of Advanced Research

    Article Title: Synergistic potentiation of the anti-metastatic effect of a Ginseng - Salvia miltiorrhiza herbal pair and its biological ingredients via the suppression of CD62E-dependent neutrophil infiltration and NETformation

    doi: 10.1016/j.jare.2024.10.036

    Figure Lengend Snippet: Rg1 reduced NETs in lung tissues. (A) Representative immunofluorescence images and quantification of H3cit and NE in the lungs (n = 3; scale bar: 50 μm). (B) Representative western blot images of H3cit and NE levels in the lungs and quantitative data ( n = 3). GAPDH was used as a loading control. (C-E) Serum levels of CXCL1, CXCL2, and G-CSF were detected via ELISA ( n = 10). (F-H) The mRNA expression levels of CXCL1, CXCL2 and G-CSF in lung tissues were determined by qRT–PCR ( n = 3). All the data are presented as mean ± SEM. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. the control group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. the model group.

    Article Snippet: The levels of mouse CXCL1, CXCL2, and G-CDF in the serum were measured via the Mouse CXCL1/KC ELISA Kit (EK296/2, Multi Sciences), Mouse CXCL2/MIP-2 ELISA Kit (EK2142, Multi Sciences), and Mouse G-CSF ELISA Kit (EK269/2, Multi Sciences) according to the manufacturers’ instructions.

    Techniques: Immunofluorescence, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR