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kb2072  (Krishgen Biosystems)


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    Krishgen Biosystems kb2072
    Kb2072, supplied by Krishgen Biosystems, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb2072/GENLISA+Mouse+Interleukin+10+(IL-10+%2F+IL10)+ELISA/10__1186_slash_s41936___026___00580___8-77-45-46
    Average 94 stars, based on 28 article reviews
    kb2072 - by Bioz Stars, 2026-09
    94/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Andrographolide mitigates lipopolysaccharide-induced mastitis in a mouse model: role of oxidative stress, inflammation, and mitochondrial dysfunction
    Article Snippet: Estimation of signalling/transcription factor in mammary homogenate Signalling factor (NF-κB) (catalogue no.-KBH11868) in tissue homogenates was estimated using commercially available ELISA kits (Krishgen biosystem, USA). .. Estimation of inflammatory cytokines in mammary homogenate and serum Mammary tissue homogenates and corresponding serum samples were used for inflammatory cytokine estimation using a commercially available ELISA kit [Tumor necrosis factor alpha (TNF-α), catalogue no.- KB2145; interleukin-6 (IL-6), catalogue no.- KB2068; interleukin-10 (IL-10), catalogue no.- KB2072], Krishgen biosystem, USA] as per manufacturers’ instructions. ..



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    Cytokine immune response in serum, the in vitro system, and tissue samples. The Th-1 (IFN-γ and IL-12) and Th-2 (IL-4 and IL-10) cytokines were quantified in the serum of immunized animals using ELISA at 2 weeks post-booster: IFN-γ (A) , IL-10 (B) , IL-12 (C) , IL-4 (D) , and IFN-γ/IL-10 ratio (E) . Twelve weeks post-infection IFN-γ (F) , IL-10 (G) , IL-12 (H) , IL-4 (I) , and IFN-γ/IL-10 ratio (J) . All experiments were performed in triplicate, having three biological replicates in each experiment and data are presented here as the mean and standard error. Different vaccinated groups were compared to determine the statistical significance of the data using ANOVA with the Tukey test analysis with p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) level of significance.

    Journal: Frontiers in Immunology

    Article Title: Mycobacterium tuberculosis antigen-containing exosomes reinforce BCG vaccine efficacy by augmenting long-term protection and memory response against experimental tuberculosis in BALB-C mice

    doi: 10.3389/fimmu.2026.1742207

    Figure Lengend Snippet: Cytokine immune response in serum, the in vitro system, and tissue samples. The Th-1 (IFN-γ and IL-12) and Th-2 (IL-4 and IL-10) cytokines were quantified in the serum of immunized animals using ELISA at 2 weeks post-booster: IFN-γ (A) , IL-10 (B) , IL-12 (C) , IL-4 (D) , and IFN-γ/IL-10 ratio (E) . Twelve weeks post-infection IFN-γ (F) , IL-10 (G) , IL-12 (H) , IL-4 (I) , and IFN-γ/IL-10 ratio (J) . All experiments were performed in triplicate, having three biological replicates in each experiment and data are presented here as the mean and standard error. Different vaccinated groups were compared to determine the statistical significance of the data using ANOVA with the Tukey test analysis with p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) level of significance.

    Article Snippet: Serum samples collected pre-immunization, post-boost, and 12 weeks post-challenge were analyzed for IgG1 (KLM1629), IgG2a (KLM1488), IFN-γ (KB2011), IL-12 (KLM0020), IL-4 (KLM0051), and IL-10 (KB2072) using GENLISA sandwich ELISA kits (Krishgen BioSystems, USA, catalog numbers as specified).

    Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Infection

    Evaluation of a functional T-cell population in splenocytes of immunized animals. The splenocytes of various vaccinated groups were isolated 12 weeks post-challenge and analyzed for Th-1 and Th-2 cytokine producing functional CD4 + T cells by FACS analysis. The representative dot plot of FACS depicts (A) CD4+IL-2, (C) CD4 + TNFα + , (E) CD4 + IL-4 + , and (G) CD4 + IL-10 + phenotypes of functional T cells. The total T-cell population of various groups was enumerated and presented here as a bar diagram; CD4+IL-2 (B) , CD4 + TNFα + (D) , CD4 + IL-4 + (F) , and CD4 + IL-10 + T cells (H) . The data were analyzed with ANOVA test and are shown as the means ( ± SD) of two independent experiments with three biological replicates in each experiment. Different vaccinated groups were compared to determine the statistical significance of the data using ANOVA with the Tukey test analysis with p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) levels.

    Journal: Frontiers in Immunology

    Article Title: Mycobacterium tuberculosis antigen-containing exosomes reinforce BCG vaccine efficacy by augmenting long-term protection and memory response against experimental tuberculosis in BALB-C mice

    doi: 10.3389/fimmu.2026.1742207

    Figure Lengend Snippet: Evaluation of a functional T-cell population in splenocytes of immunized animals. The splenocytes of various vaccinated groups were isolated 12 weeks post-challenge and analyzed for Th-1 and Th-2 cytokine producing functional CD4 + T cells by FACS analysis. The representative dot plot of FACS depicts (A) CD4+IL-2, (C) CD4 + TNFα + , (E) CD4 + IL-4 + , and (G) CD4 + IL-10 + phenotypes of functional T cells. The total T-cell population of various groups was enumerated and presented here as a bar diagram; CD4+IL-2 (B) , CD4 + TNFα + (D) , CD4 + IL-4 + (F) , and CD4 + IL-10 + T cells (H) . The data were analyzed with ANOVA test and are shown as the means ( ± SD) of two independent experiments with three biological replicates in each experiment. Different vaccinated groups were compared to determine the statistical significance of the data using ANOVA with the Tukey test analysis with p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) levels.

    Article Snippet: Serum samples collected pre-immunization, post-boost, and 12 weeks post-challenge were analyzed for IgG1 (KLM1629), IgG2a (KLM1488), IFN-γ (KB2011), IL-12 (KLM0020), IL-4 (KLM0051), and IL-10 (KB2072) using GENLISA sandwich ELISA kits (Krishgen BioSystems, USA, catalog numbers as specified).

    Techniques: Functional Assay, Isolation