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pe conjugated igg2a antibody  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec pe conjugated igg2a antibody
    Pe Conjugated Igg2a Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isotype+control/Isotype+Control+Antibody%2C+mouse+IgG2a/pm42929851-76-4-13
    Average 95 stars, based on 76 article reviews
    pe conjugated igg2a antibody - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Marker:

    Article Title: Establishment of a 3D Multicellular HCC Tumor Spheroid Model to Unravel Nrf2’s Influence on the Tumor Immune Microenvironment
    Article Snippet: .. The macrophage surface marker CD11b was stained using VioGreen anti-CD11b (#130-113-811, Miltenyi Biotec, Bergisch Gladbach, Germany); REA Control-VioGreen was used as an isotype control (#130-113-456, Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Establishment of a 3D Multicellular HCC Tumor Spheroid Model to Unravel Nrf2's Influence on the Tumor Immune Microenvironment.
    Article Snippet: .. The macrophage surface marker CD11b was stained using VioGreen anti-CD11b (#130-113-811, Miltenyi Biotec, Bergisch Gladbach, Germany); REA Control-VioGreen was used as an isotype control (#130-113-456, Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Staining:

    Article Title: Establishment of a 3D Multicellular HCC Tumor Spheroid Model to Unravel Nrf2’s Influence on the Tumor Immune Microenvironment
    Article Snippet: .. The macrophage surface marker CD11b was stained using VioGreen anti-CD11b (#130-113-811, Miltenyi Biotec, Bergisch Gladbach, Germany); REA Control-VioGreen was used as an isotype control (#130-113-456, Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130–113–831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552,843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130–113–831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130-113-831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130-113-831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Article Title: Immortalized human hair follicle-derived mesenchymal-like stromal cells for the long-term production of scalable Immunomodulatory and regenerative secretome
    Article Snippet: .. For the former, cells were stained with either anti-CD56-FITC (Miltenyi Biotec, 130-114-740) at a 1:200 dilution or the corresponding isotype control (Miltenyi Biotec, 130-113-437) following manufacturer instructions and acquired using a MACSQuant ® analyzer (Miltenyi Biotec). .. Data were analyzed with FlowJoTM v10.6.2 Software (BD Life Sciences).

    Article Title: Enhanced Workflow for Urinary Extracellular Vesicle Isolation Using Membrane-Sensing Peptides
    Article Snippet: .. Parallelly, for each sample analyzed, unstained EVs, as well as EVs stained with the appropriate isotype control (Cat. No. 130-113-438, Miltenyi Biotec) at the same concentration of PE-anti-CD9 antibody. ..

    Article Title: Establishment of a 3D Multicellular HCC Tumor Spheroid Model to Unravel Nrf2's Influence on the Tumor Immune Microenvironment.
    Article Snippet: .. The macrophage surface marker CD11b was stained using VioGreen anti-CD11b (#130-113-811, Miltenyi Biotec, Bergisch Gladbach, Germany); REA Control-VioGreen was used as an isotype control (#130-113-456, Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Control:

    Article Title: Establishment of a 3D Multicellular HCC Tumor Spheroid Model to Unravel Nrf2’s Influence on the Tumor Immune Microenvironment
    Article Snippet: .. The macrophage surface marker CD11b was stained using VioGreen anti-CD11b (#130-113-811, Miltenyi Biotec, Bergisch Gladbach, Germany); REA Control-VioGreen was used as an isotype control (#130-113-456, Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130–113–831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552,843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130–113–831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Article Title: CytoLight: A Rapid and Versatile Fluorescent-Based Labeling Method for Extracellular Vesicle Characterization and Tracking
    Article Snippet: For colocalization experiments, ZetaNavigator (version 1.4.7.6, Particle Metrix) was used for acquisition, and ParticleExplorer (version 4.3.4.4, Particle Metrix) was used for analysis. .. For surface phenotyping, 2μl of anti-CD81 antibody (anti-human, Vio® Bright B515, REAfinity, Miltenyi Biotec, Germany) or isotype control (REA control antibody, human IgG1, Vio® Bright B515, Miltenyi Biotec) were added to 10μl of PBS with or without EVs and incubated overnight at 4°C in the dark. .. EV-FCM was performed using either CytoFLEX nano flow cytometer (Beckman Coulter) or ImageStreamX Mk II imaging flow cytometer (Cytek Biosciences, USA).

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130-113-831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130-113-831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Article Title: Immortalized human hair follicle-derived mesenchymal-like stromal cells for the long-term production of scalable Immunomodulatory and regenerative secretome
    Article Snippet: .. For the former, cells were stained with either anti-CD56-FITC (Miltenyi Biotec, 130-114-740) at a 1:200 dilution or the corresponding isotype control (Miltenyi Biotec, 130-113-437) following manufacturer instructions and acquired using a MACSQuant ® analyzer (Miltenyi Biotec). .. Data were analyzed with FlowJoTM v10.6.2 Software (BD Life Sciences).

    Article Title: Single-cell transcriptomics-informed induced pluripotent stem cells differentiation to tenogenic lineage
    Article Snippet: For assessment of DLL-1 levels to determine induction efficiency, cells that were induced for 4 days were lifted using Accutase for 5 min at 37°C and washed 3x with FACS buffer containing 2% bovine serum albumin (BSA, A4503, Sigma, St. Louis, MO) and 0.1% sodium azide (S2002, Sigma) in PBS. .. The cells were either unstained, labeled with DLL1 anti-human antibody (APC-Vio 770, #130-106-148, Miltenyi Biotec, Bergisch Gladbach), or labeled with its associated isotype control (APC-Vio 770, #130-113-759, Miltenyi Biotec) for 15 min protected from light at 4°C. ..

    Article Title: Enhanced Workflow for Urinary Extracellular Vesicle Isolation Using Membrane-Sensing Peptides
    Article Snippet: .. Parallelly, for each sample analyzed, unstained EVs, as well as EVs stained with the appropriate isotype control (Cat. No. 130-113-438, Miltenyi Biotec) at the same concentration of PE-anti-CD9 antibody. ..

    Article Title: Establishment of a 3D Multicellular HCC Tumor Spheroid Model to Unravel Nrf2's Influence on the Tumor Immune Microenvironment.
    Article Snippet: .. The macrophage surface marker CD11b was stained using VioGreen anti-CD11b (#130-113-811, Miltenyi Biotec, Bergisch Gladbach, Germany); REA Control-VioGreen was used as an isotype control (#130-113-456, Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    FACS:

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130–113–831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552,843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130–113–831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130-113-831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130-113-831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Analysis:

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130–113–831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552,843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130–113–831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Fluorescence:

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130–113–831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552,843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130–113–831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130-113-831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130-113-831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Negative Control:

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130–113–831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552,843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130–113–831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130-113-831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130-113-831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Positive Control:

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130–113–831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552,843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130–113–831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: For isotype control, mouse IgG2a, APC antibody (Miltenyi Biotec, cat. 130-113-831) was used. .. FACS analysis and sorting were performed with the following controls: fluorescence compensation (CompBeads Anti-Mouse Ig, κ Negative Control Compensation Particles Set, BD Biosciences #552843), isotype control (mouse antibody IgG2a, APC, 1:400, Miltenyi Biotec, #130-113-831), positive control with the peritoneal cavity cells stained with specific antibodies, and negative control with unstained brain cells. ..

    Incubation:

    Article Title: CytoLight: A Rapid and Versatile Fluorescent-Based Labeling Method for Extracellular Vesicle Characterization and Tracking
    Article Snippet: For colocalization experiments, ZetaNavigator (version 1.4.7.6, Particle Metrix) was used for acquisition, and ParticleExplorer (version 4.3.4.4, Particle Metrix) was used for analysis. .. For surface phenotyping, 2μl of anti-CD81 antibody (anti-human, Vio® Bright B515, REAfinity, Miltenyi Biotec, Germany) or isotype control (REA control antibody, human IgG1, Vio® Bright B515, Miltenyi Biotec) were added to 10μl of PBS with or without EVs and incubated overnight at 4°C in the dark. .. EV-FCM was performed using either CytoFLEX nano flow cytometer (Beckman Coulter) or ImageStreamX Mk II imaging flow cytometer (Cytek Biosciences, USA).

    Labeling:

    Article Title: Single-cell transcriptomics-informed induced pluripotent stem cells differentiation to tenogenic lineage
    Article Snippet: For assessment of DLL-1 levels to determine induction efficiency, cells that were induced for 4 days were lifted using Accutase for 5 min at 37°C and washed 3x with FACS buffer containing 2% bovine serum albumin (BSA, A4503, Sigma, St. Louis, MO) and 0.1% sodium azide (S2002, Sigma) in PBS. .. The cells were either unstained, labeled with DLL1 anti-human antibody (APC-Vio 770, #130-106-148, Miltenyi Biotec, Bergisch Gladbach), or labeled with its associated isotype control (APC-Vio 770, #130-113-759, Miltenyi Biotec) for 15 min protected from light at 4°C. ..

    Concentration Assay:

    Article Title: Enhanced Workflow for Urinary Extracellular Vesicle Isolation Using Membrane-Sensing Peptides
    Article Snippet: .. Parallelly, for each sample analyzed, unstained EVs, as well as EVs stained with the appropriate isotype control (Cat. No. 130-113-438, Miltenyi Biotec) at the same concentration of PE-anti-CD9 antibody. ..



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    ( A ) Western blot detection of PD-1 protein expression; ( B ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( C–E ) IL-6 ( C ), IL-12 ( D ), and TNF-α ( E ) levels measured by ELISA; (F-H) Flow-cytometric detection of IL-6( F ), IL-12( G ), and TNF-α(H) proportions; ( I ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( J ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.
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    Image Search Results


    ( A ) Western blot detection of PD-1 protein expression; ( B ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( C–E ) IL-6 ( C ), IL-12 ( D ), and TNF-α ( E ) levels measured by ELISA; (F-H) Flow-cytometric detection of IL-6( F ), IL-12( G ), and TNF-α(H) proportions; ( I ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( J ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.

    Journal: Journal of Microbiology and Biotechnology

    Article Title: Anti-PD-1 Therapy Alleviates Klebsiella pneumoniae -Induced Liver Abscess by Effectively Activating Kupffer Cells

    doi: 10.4014/jmb.2602.02040

    Figure Lengend Snippet: ( A ) Western blot detection of PD-1 protein expression; ( B ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( C–E ) IL-6 ( C ), IL-12 ( D ), and TNF-α ( E ) levels measured by ELISA; (F-H) Flow-cytometric detection of IL-6( F ), IL-12( G ), and TNF-α(H) proportions; ( I ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( J ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.

    Article Snippet: Simultaneously with bacterial inoculation, mice were intraperitoneally injected with 200 μg anti-PD-1 monoclonal antibody (CP151, BioXCell, USA) or an equivalent amount of mouse IgG2a isotype control antibody (BE0085, BioXCell, USA).

    Techniques: Western Blot, Expressing, In Vitro, Activity Assay, Bacteria, Enzyme-linked Immunosorbent Assay, Analysis

    ( A ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( B–D ) IL-6 ( B ), IL-12 ( C ), and TNF-α ( D ) levels measured by ELISA; ( E-G ) Flow cytometry detection of IL-6 ( E ), IL-12 ( F ), and TNF-α ( G ) proportions; ( H ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( I ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.

    Journal: Journal of Microbiology and Biotechnology

    Article Title: Anti-PD-1 Therapy Alleviates Klebsiella pneumoniae -Induced Liver Abscess by Effectively Activating Kupffer Cells

    doi: 10.4014/jmb.2602.02040

    Figure Lengend Snippet: ( A ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( B–D ) IL-6 ( B ), IL-12 ( C ), and TNF-α ( D ) levels measured by ELISA; ( E-G ) Flow cytometry detection of IL-6 ( E ), IL-12 ( F ), and TNF-α ( G ) proportions; ( H ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( I ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.

    Article Snippet: Simultaneously with bacterial inoculation, mice were intraperitoneally injected with 200 μg anti-PD-1 monoclonal antibody (CP151, BioXCell, USA) or an equivalent amount of mouse IgG2a isotype control antibody (BE0085, BioXCell, USA).

    Techniques: In Vitro, Activity Assay, Bacteria, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Analysis, Western Blot

    ( A ) LA severity ameliorated after PD-1 antibody treatment; ( B ) HE staining of mouse liver and microscopic observation; ( C–D ) K. pneumoniae burden in liver ( C ) and blood ( D ) of infected mice. ( E–F ) ELISA detection of mouse serum ALT ( E ) and AST ( F ) levels. * P < 0.05 was considered statistically significant.

    Journal: Journal of Microbiology and Biotechnology

    Article Title: Anti-PD-1 Therapy Alleviates Klebsiella pneumoniae -Induced Liver Abscess by Effectively Activating Kupffer Cells

    doi: 10.4014/jmb.2602.02040

    Figure Lengend Snippet: ( A ) LA severity ameliorated after PD-1 antibody treatment; ( B ) HE staining of mouse liver and microscopic observation; ( C–D ) K. pneumoniae burden in liver ( C ) and blood ( D ) of infected mice. ( E–F ) ELISA detection of mouse serum ALT ( E ) and AST ( F ) levels. * P < 0.05 was considered statistically significant.

    Article Snippet: Simultaneously with bacterial inoculation, mice were intraperitoneally injected with 200 μg anti-PD-1 monoclonal antibody (CP151, BioXCell, USA) or an equivalent amount of mouse IgG2a isotype control antibody (BE0085, BioXCell, USA).

    Techniques: H&E Stain, Infection, Enzyme-linked Immunosorbent Assay