Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Geraniin attenuates isoproterenol-induced cardiac hypertrophy by inhibiting inflammation, oxidative stress and cellular apoptosis

doi: 10.4196/kjpp.24.200

Figure Lengend Snippet: (A) The chemical structure of geraniin. (B) Representative images showing that geraniin treatment, similar to SPI (a positive control), suppressed ISO-induced increase in cardiac volume in mice. (C–F) Quantitative analysis of the effects of geraniin and SPI treatment on the ratio of heart weight (HW) and left ventricular weight (LVW) to body weight (BW) (C, D) and the ratio of HW and LVW to tibial length (TL) (E, F) (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO) in ISO-treated mice. (G, H) Quantitative analysis of the effects of geraniin and SPI on mRNA expression of ANP (G) and BNP (H) in mice treated with ISO (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (I–K) Representative images and quantitative analysis indicating the effects of geraniin and SPI on the protein expression of ANP (I, J) and BNP (I, K) in ISO-induced mouse hypertrophic cardiac tissue (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). Data are shown as mean ± SEM. ISO, isoproterenol; SPI, spironolactone; ANP, atrial natriuretic polypeptide; BNP, brain natriuretic peptide; Con, control.

Article Snippet: Geraniin (#HY-N0472), ISO (#HY-N1952) and spironolactone (SPI) (#HY-B0561) were purchased from MedChem Express.

Techniques: Positive Control, Control, Expressing

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Geraniin attenuates isoproterenol-induced cardiac hypertrophy by inhibiting inflammation, oxidative stress and cellular apoptosis

doi: 10.4196/kjpp.24.200

Figure Lengend Snippet: (A, B) Quantitative analysis of the effects of geraniin and SPI on the mRNA expression of collagen I á1 and collagen III á1 in hypertrophic cardiac tissue of mice (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (C, D) Representative images (C) and quantitative analysis (D) of the impact of geraniin and SPI on cardiomyocyte fibrosis area (CSA) as detected by the wheat germ agglutinin (WGA) staining of hypertrophic cardiac tissue (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). Scale bar: 20 μm. (E–G) Representative images and quantitative analysis of the effects of geraniin and SPI on collagen I (E, F) and collagen III (E, G) expression (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (H) Representative HE staining images of the left ventricle in mice treated with ISO and geraniin or SPI. Scale bar: 20 μm. Data are shown as mean ± SEM. ISO, isoproterenol; SPI, spironolactone; HE, hematoxylin and eosin; Con, control.

Article Snippet: Geraniin (#HY-N0472), ISO (#HY-N1952) and spironolactone (SPI) (#HY-B0561) were purchased from MedChem Express.

Techniques: Expressing, Control, Staining

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Geraniin attenuates isoproterenol-induced cardiac hypertrophy by inhibiting inflammation, oxidative stress and cellular apoptosis

doi: 10.4196/kjpp.24.200

Figure Lengend Snippet: (A–D) Quantitative analysis of the effects of geraniin and SPI son the mRNA expression levels of IL-1β (A), IL-6 (B), and TNF-α (C) and mRNA expression levels of IL-10 (D) in hypertrophic heart tissues of mice (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (E–I) Representative images and quantitative analysis of the impact of geraniin and SPI on the protein expression of IL-1β (E, F), IL-6 (E, G), TNF-α (E, H), and IL-10 (E, I) (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). Data are shown as mean ± SEM. ISO, isoproterenol; SPI, spironolactone; IL, interleukin; TNF-α, tumor necrosis factor-α; Con, control.

Article Snippet: Geraniin (#HY-N0472), ISO (#HY-N1952) and spironolactone (SPI) (#HY-B0561) were purchased from MedChem Express.

Techniques: Expressing, Control

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Geraniin attenuates isoproterenol-induced cardiac hypertrophy by inhibiting inflammation, oxidative stress and cellular apoptosis

doi: 10.4196/kjpp.24.200

Figure Lengend Snippet: (A, B) Quantitative analysis of the effects of geraniin and SPI on MDA (A) and NO (B) (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (C–E) Quantitative analysis of the impact of geraniin and SPI on T-AOC (C), SOD (D), and GSH (E) in hypertrophic heart tissue of mice (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (F, G) Representative images and quantitative analysis of the results of ROS assays indicating the effects of geraniin on ISO-induced increase in ROS production in hypertrophic heart tissue of mice (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). Scale bar: 20 μm. Data are shown as mean ± SEM. ISO, isoproterenol; SPI, spironolactone; MDA, malondialdehyde; NO, nitric oxide; T-AOC, total antioxidant capacity; SOD, superoxide dismutase; GSH, glutathione; ROS, reactive oxygen species; Con, control.

Article Snippet: Geraniin (#HY-N0472), ISO (#HY-N1952) and spironolactone (SPI) (#HY-B0561) were purchased from MedChem Express.

Techniques: Control

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Geraniin attenuates isoproterenol-induced cardiac hypertrophy by inhibiting inflammation, oxidative stress and cellular apoptosis

doi: 10.4196/kjpp.24.200

Figure Lengend Snippet: (A–E) Representative images and quantitative analysis for the effect of geraniin and SPI on Bax (A, B), caspase-3 (A, C), and caspase-9 (A, D) and on Bcl-2 (A, E) in the hypertrophic heart tissue of mice (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (F, G) Representative images and quantitative analysis of the results of TUNEL staining of hypertrophic heart tissue in the indicated groups (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). Scale bar: 20 μm. Data are shown as mean ± SEM. ISO, isoproterenol; SPI, spironolactone; TUNEL, TdT-mediated dUTP nick-end labeling; Con, control.

Article Snippet: Geraniin (#HY-N0472), ISO (#HY-N1952) and spironolactone (SPI) (#HY-B0561) were purchased from MedChem Express.

Techniques: Control, TUNEL Assay, Staining, End Labeling

Journal: iScience

Article Title: Semi-rational design of an aromatic dioxygenase by substrate tunnel redirection

doi: 10.1016/j.isci.2024.111570

Figure Lengend Snippet:

Article Snippet: Isoeugenol , Macklin , Cat#I794535.

Techniques: Virus, Recombinant, Cloning, Marker, Sequencing, Plasmid Preparation, Software

Journal: The Malaysian Journal of Medical Sciences : MJMS

Article Title: In Vitro Anticancer Potential of Eugenol on Oral Cancer Cell Lines: A Systematic Review

doi: 10.21315/mjms2024.31.5.2

Figure Lengend Snippet: Main characteristics of the reviewed articles

Article Snippet: 13 , Fujisawa et al. (Japan, 2004) ( ) , HSG , , Eugenol Isoeugenol Bis-eugenol (synthetic) , Solution, liquid (Tokyo Kasei Chem. Co., Tokyo, Japan and synthesised) , MTT assay , ~0.2 mM , , 24 h , , (Not measured) , Eugenol, bis-eugenol, and iso-eugenol were cytotoxic to HSG cell line but did not induce ROS production..

Techniques: Marker, MTT Assay, Membrane, Staining, DNA Fragmentation Assay, Flow Cytometry, Inhibition, Western Blot, Produced, Concentration Assay, Electrophoresis, Single Cell Gel Electrophoresis, Cytotoxicity Assay, Irradiation, TUNEL Assay