Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Geraniin attenuates isoproterenol-induced cardiac hypertrophy by inhibiting inflammation, oxidative stress and cellular apoptosis

doi: 10.4196/kjpp.24.200

Figure Lengend Snippet: (A) The chemical structure of geraniin. (B) Representative images showing that geraniin treatment, similar to SPI (a positive control), suppressed ISO-induced increase in cardiac volume in mice. (C–F) Quantitative analysis of the effects of geraniin and SPI treatment on the ratio of heart weight (HW) and left ventricular weight (LVW) to body weight (BW) (C, D) and the ratio of HW and LVW to tibial length (TL) (E, F) (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO) in ISO-treated mice. (G, H) Quantitative analysis of the effects of geraniin and SPI on mRNA expression of ANP (G) and BNP (H) in mice treated with ISO (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (I–K) Representative images and quantitative analysis indicating the effects of geraniin and SPI on the protein expression of ANP (I, J) and BNP (I, K) in ISO-induced mouse hypertrophic cardiac tissue (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). Data are shown as mean ± SEM. ISO, isoproterenol; SPI, spironolactone; ANP, atrial natriuretic polypeptide; BNP, brain natriuretic peptide; Con, control.

Article Snippet: Geraniin (#HY-N0472), ISO (#HY-N1952) and spironolactone (SPI) (#HY-B0561) were purchased from MedChem Express.

Techniques: Positive Control, Control, Expressing

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Geraniin attenuates isoproterenol-induced cardiac hypertrophy by inhibiting inflammation, oxidative stress and cellular apoptosis

doi: 10.4196/kjpp.24.200

Figure Lengend Snippet: (A, B) Quantitative analysis of the effects of geraniin and SPI on the mRNA expression of collagen I á1 and collagen III á1 in hypertrophic cardiac tissue of mice (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (C, D) Representative images (C) and quantitative analysis (D) of the impact of geraniin and SPI on cardiomyocyte fibrosis area (CSA) as detected by the wheat germ agglutinin (WGA) staining of hypertrophic cardiac tissue (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). Scale bar: 20 μm. (E–G) Representative images and quantitative analysis of the effects of geraniin and SPI on collagen I (E, F) and collagen III (E, G) expression (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (H) Representative HE staining images of the left ventricle in mice treated with ISO and geraniin or SPI. Scale bar: 20 μm. Data are shown as mean ± SEM. ISO, isoproterenol; SPI, spironolactone; HE, hematoxylin and eosin; Con, control.

Article Snippet: Geraniin (#HY-N0472), ISO (#HY-N1952) and spironolactone (SPI) (#HY-B0561) were purchased from MedChem Express.

Techniques: Expressing, Control, Staining

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Geraniin attenuates isoproterenol-induced cardiac hypertrophy by inhibiting inflammation, oxidative stress and cellular apoptosis

doi: 10.4196/kjpp.24.200

Figure Lengend Snippet: (A–D) Quantitative analysis of the effects of geraniin and SPI son the mRNA expression levels of IL-1β (A), IL-6 (B), and TNF-α (C) and mRNA expression levels of IL-10 (D) in hypertrophic heart tissues of mice (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (E–I) Representative images and quantitative analysis of the impact of geraniin and SPI on the protein expression of IL-1β (E, F), IL-6 (E, G), TNF-α (E, H), and IL-10 (E, I) (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). Data are shown as mean ± SEM. ISO, isoproterenol; SPI, spironolactone; IL, interleukin; TNF-α, tumor necrosis factor-α; Con, control.

Article Snippet: Geraniin (#HY-N0472), ISO (#HY-N1952) and spironolactone (SPI) (#HY-B0561) were purchased from MedChem Express.

Techniques: Expressing, Control

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Geraniin attenuates isoproterenol-induced cardiac hypertrophy by inhibiting inflammation, oxidative stress and cellular apoptosis

doi: 10.4196/kjpp.24.200

Figure Lengend Snippet: (A, B) Quantitative analysis of the effects of geraniin and SPI on MDA (A) and NO (B) (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (C–E) Quantitative analysis of the impact of geraniin and SPI on T-AOC (C), SOD (D), and GSH (E) in hypertrophic heart tissue of mice (n = 8, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (F, G) Representative images and quantitative analysis of the results of ROS assays indicating the effects of geraniin on ISO-induced increase in ROS production in hypertrophic heart tissue of mice (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). Scale bar: 20 μm. Data are shown as mean ± SEM. ISO, isoproterenol; SPI, spironolactone; MDA, malondialdehyde; NO, nitric oxide; T-AOC, total antioxidant capacity; SOD, superoxide dismutase; GSH, glutathione; ROS, reactive oxygen species; Con, control.

Article Snippet: Geraniin (#HY-N0472), ISO (#HY-N1952) and spironolactone (SPI) (#HY-B0561) were purchased from MedChem Express.

Techniques: Control

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Geraniin attenuates isoproterenol-induced cardiac hypertrophy by inhibiting inflammation, oxidative stress and cellular apoptosis

doi: 10.4196/kjpp.24.200

Figure Lengend Snippet: (A–E) Representative images and quantitative analysis for the effect of geraniin and SPI on Bax (A, B), caspase-3 (A, C), and caspase-9 (A, D) and on Bcl-2 (A, E) in the hypertrophic heart tissue of mice (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). (F, G) Representative images and quantitative analysis of the results of TUNEL staining of hypertrophic heart tissue in the indicated groups (n = 5, *p < 0.05 vs . control, # p < 0.05 vs . ISO). Scale bar: 20 μm. Data are shown as mean ± SEM. ISO, isoproterenol; SPI, spironolactone; TUNEL, TdT-mediated dUTP nick-end labeling; Con, control.

Article Snippet: Geraniin (#HY-N0472), ISO (#HY-N1952) and spironolactone (SPI) (#HY-B0561) were purchased from MedChem Express.

Techniques: Control, TUNEL Assay, Staining, End Labeling

Journal: International Journal of Molecular Sciences

Article Title: Oxidation of Isoeugenol by Salen Complexes with Bulky Substituents

doi: 10.3390/ijms11030912

Figure Lengend Snippet: Isoeugenol: 2-methoxy-4-[( Z )-1-propenyl] phenol ( 3 ) and 2-methoxy-4-[( E )-1-propenyl] phenol ( 4 ).

Article Snippet: Isoeugenol (99%, mixture of cis and trans), ethanol (99.9%) and ethyl acetate (99.9%) were purchased from Fluka.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Oxidation of Isoeugenol by Salen Complexes with Bulky Substituents

doi: 10.3390/ijms11030912

Figure Lengend Snippet: Results of GPC analyses on oxidation products of isoeugenol with different catalyst.

Article Snippet: Isoeugenol (99%, mixture of cis and trans), ethanol (99.9%) and ethyl acetate (99.9%) were purchased from Fluka.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Oxidation of Isoeugenol by Salen Complexes with Bulky Substituents

doi: 10.3390/ijms11030912

Figure Lengend Snippet: Main oxidation products of isoeugenol: vanillin ( 5 ), acetovanillone ( 6 ) and vanillylmandelic acid ( 7 ), as detected by GC-MS analysis.

Article Snippet: Isoeugenol (99%, mixture of cis and trans), ethanol (99.9%) and ethyl acetate (99.9%) were purchased from Fluka.

Techniques: Gas Chromatography-Mass Spectrometry

Journal: International Journal of Molecular Sciences

Article Title: Oxidation of Isoeugenol by Salen Complexes with Bulky Substituents

doi: 10.3390/ijms11030912

Figure Lengend Snippet: Results of GPC analyses on the oxidation products of isoeugenol with CuSS at different pH.

Article Snippet: Isoeugenol (99%, mixture of cis and trans), ethanol (99.9%) and ethyl acetate (99.9%) were purchased from Fluka.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Oxidation of Isoeugenol by Salen Complexes with Bulky Substituents

doi: 10.3390/ijms11030912

Figure Lengend Snippet: Results of GPC analyses on oxidation products of isoeugenol applying Zutropf (ZT) and Zuluaf (ZL) conditions.

Article Snippet: Isoeugenol (99%, mixture of cis and trans), ethanol (99.9%) and ethyl acetate (99.9%) were purchased from Fluka.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Oxidation of Isoeugenol by Salen Complexes with Bulky Substituents

doi: 10.3390/ijms11030912

Figure Lengend Snippet: Results of GPC analyses on coupling products of isoeugenol with CuSS at different concentrations of catalyst and monomer.

Article Snippet: Isoeugenol (99%, mixture of cis and trans), ethanol (99.9%) and ethyl acetate (99.9%) were purchased from Fluka.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Oxidation of Isoeugenol by Salen Complexes with Bulky Substituents

doi: 10.3390/ijms11030912

Figure Lengend Snippet: Results of GPC analyses on coupling products of isoeugenol in the presence of CuSS as catalyst when the oxidant concentration and the oxidant addition rate were varied.

Article Snippet: Isoeugenol (99%, mixture of cis and trans), ethanol (99.9%) and ethyl acetate (99.9%) were purchased from Fluka.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Oxidation of Isoeugenol by Salen Complexes with Bulky Substituents

doi: 10.3390/ijms11030912

Figure Lengend Snippet: 13 C-NMR spectrum of the acetylated isoeugenol DHP and chemical structure of principal intermonomeric linkages detected (β-5, β- O -4).

Article Snippet: Isoeugenol (99%, mixture of cis and trans), ethanol (99.9%) and ethyl acetate (99.9%) were purchased from Fluka.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Oxidation of Isoeugenol by Salen Complexes with Bulky Substituents

doi: 10.3390/ijms11030912

Figure Lengend Snippet: LC-MS spectrum of isoeugenol DHP.

Article Snippet: Isoeugenol (99%, mixture of cis and trans), ethanol (99.9%) and ethyl acetate (99.9%) were purchased from Fluka.

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: International Journal of Molecular Sciences

Article Title: Oxidation of Isoeugenol by Salen Complexes with Bulky Substituents

doi: 10.3390/ijms11030912

Figure Lengend Snippet: Representative “lignin-like” product of isoeugenol polymerization.

Article Snippet: Isoeugenol (99%, mixture of cis and trans), ethanol (99.9%) and ethyl acetate (99.9%) were purchased from Fluka.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Oxidation of Isoeugenol by Salen Complexes with Bulky Substituents

doi: 10.3390/ijms11030912

Figure Lengend Snippet: Scavenging activity of isoeugenol and DHP toward DPPH.

Article Snippet: Isoeugenol (99%, mixture of cis and trans), ethanol (99.9%) and ethyl acetate (99.9%) were purchased from Fluka.

Techniques: Activity Assay

Journal: iScience

Article Title: Semi-rational design of an aromatic dioxygenase by substrate tunnel redirection

doi: 10.1016/j.isci.2024.111570

Figure Lengend Snippet:

Article Snippet: Isoeugenol , Macklin , Cat#I794535.

Techniques: Virus, Recombinant, Cloning, Marker, Sequencing, Plasmid Preparation, Software