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h2b irfp fusion sequence  (Addgene inc)


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    Structured Review

    Addgene inc h2b irfp fusion sequence
    H2b Irfp Fusion Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/irfp+sequence/pLenti-H2B-iRFP720+(Plasmid+%23128961)/bio_rxiv__64898__2026__01__08__698406-257-4-8
    Average 94 stars, based on 9 article reviews
    h2b irfp fusion sequence - by Bioz Stars, 2026-11
    94/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins.
    Article Snippet: .. To generate the iRFP-CaaX transfer plasmid, the iRFP sequence was amplified fromDEST-H2B-iRFP670 (Addgene #90237) and cloned into a pHR lentiviral backbone upstream of a C-terminal CaaX sequence. .. Protein expression and purification For all proteins, bacteria were cultured in 2YT autoinduction media including trace elements (Formedium; Norfolk, United Kingdom).

    Article Title: An inducible system for in vitro and in vivo Fas activation using FKBP-FRB-rapamycin complex.
    Article Snippet: The inducible activation system is valuable for investigating spatiotemporal roles of molecules.. A chemically inducible activation system for Fas (CD95/APO-1), which works efficiently to induce apoptosis and leads non-apoptotic pathways, has not yet been developed.. Here, we engineered a rapamycininduced dimerization system of Fas consisting of FKBP and FRB proteins.

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins
    Article Snippet: .. To generate the iRFP-CaaX transfer plasmid, the iRFP sequence was amplified from DEST-H2B-iRFP670 (Addgene #90237) and cloned into a pHR lentiviral backbone upstream of a C-terminal CaaX sequence. .. For all proteins, bacteria were cultured in 2YT autoinduction media including trace elements (Formedium; Norfolk, United Kingdom).

    Sequencing:

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins.
    Article Snippet: .. To generate the iRFP-CaaX transfer plasmid, the iRFP sequence was amplified fromDEST-H2B-iRFP670 (Addgene #90237) and cloned into a pHR lentiviral backbone upstream of a C-terminal CaaX sequence. .. Protein expression and purification For all proteins, bacteria were cultured in 2YT autoinduction media including trace elements (Formedium; Norfolk, United Kingdom).

    Article Title: An inducible system for in vitro and in vivo Fas activation using FKBP-FRB-rapamycin complex.
    Article Snippet: The inducible activation system is valuable for investigating spatiotemporal roles of molecules.. A chemically inducible activation system for Fas (CD95/APO-1), which works efficiently to induce apoptosis and leads non-apoptotic pathways, has not yet been developed.. Here, we engineered a rapamycininduced dimerization system of Fas consisting of FKBP and FRB proteins.

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins
    Article Snippet: .. To generate the iRFP-CaaX transfer plasmid, the iRFP sequence was amplified from DEST-H2B-iRFP670 (Addgene #90237) and cloned into a pHR lentiviral backbone upstream of a C-terminal CaaX sequence. .. For all proteins, bacteria were cultured in 2YT autoinduction media including trace elements (Formedium; Norfolk, United Kingdom).

    Amplification:

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins.
    Article Snippet: .. To generate the iRFP-CaaX transfer plasmid, the iRFP sequence was amplified fromDEST-H2B-iRFP670 (Addgene #90237) and cloned into a pHR lentiviral backbone upstream of a C-terminal CaaX sequence. .. Protein expression and purification For all proteins, bacteria were cultured in 2YT autoinduction media including trace elements (Formedium; Norfolk, United Kingdom).

    Article Title: An inducible system for in vitro and in vivo Fas activation using FKBP-FRB-rapamycin complex.
    Article Snippet: The inducible activation system is valuable for investigating spatiotemporal roles of molecules.. A chemically inducible activation system for Fas (CD95/APO-1), which works efficiently to induce apoptosis and leads non-apoptotic pathways, has not yet been developed.. Here, we engineered a rapamycininduced dimerization system of Fas consisting of FKBP and FRB proteins.

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins
    Article Snippet: .. To generate the iRFP-CaaX transfer plasmid, the iRFP sequence was amplified from DEST-H2B-iRFP670 (Addgene #90237) and cloned into a pHR lentiviral backbone upstream of a C-terminal CaaX sequence. .. For all proteins, bacteria were cultured in 2YT autoinduction media including trace elements (Formedium; Norfolk, United Kingdom).

    Clone Assay:

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins.
    Article Snippet: .. To generate the iRFP-CaaX transfer plasmid, the iRFP sequence was amplified fromDEST-H2B-iRFP670 (Addgene #90237) and cloned into a pHR lentiviral backbone upstream of a C-terminal CaaX sequence. .. Protein expression and purification For all proteins, bacteria were cultured in 2YT autoinduction media including trace elements (Formedium; Norfolk, United Kingdom).

    Article Title: Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins
    Article Snippet: .. To generate the iRFP-CaaX transfer plasmid, the iRFP sequence was amplified from DEST-H2B-iRFP670 (Addgene #90237) and cloned into a pHR lentiviral backbone upstream of a C-terminal CaaX sequence. .. For all proteins, bacteria were cultured in 2YT autoinduction media including trace elements (Formedium; Norfolk, United Kingdom).

    Construct:

    Article Title: An inducible system for in vitro and in vivo Fas activation using FKBP-FRB-rapamycin complex.
    Article Snippet: The inducible activation system is valuable for investigating spatiotemporal roles of molecules.. A chemically inducible activation system for Fas (CD95/APO-1), which works efficiently to induce apoptosis and leads non-apoptotic pathways, has not yet been developed.. Here, we engineered a rapamycininduced dimerization system of Fas consisting of FKBP and FRB proteins.

    Polymerase Chain Reaction:

    Article Title: An inducible system for in vitro and in vivo Fas activation using FKBP-FRB-rapamycin complex.
    Article Snippet: The inducible activation system is valuable for investigating spatiotemporal roles of molecules.. A chemically inducible activation system for Fas (CD95/APO-1), which works efficiently to induce apoptosis and leads non-apoptotic pathways, has not yet been developed.. Here, we engineered a rapamycininduced dimerization system of Fas consisting of FKBP and FRB proteins.



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    Image Search Results


    HeLa cells expressing eMagA-mCherry-Mito (green) and Lys-eMagB-iRFP (magenta). Scale bar: 0.5 μm.

    Journal: eLife

    Article Title: Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells

    doi: 10.7554/eLife.63230

    Figure Lengend Snippet: HeLa cells expressing eMagA-mCherry-Mito (green) and Lys-eMagB-iRFP (magenta). Scale bar: 0.5 μm.

    Article Snippet: PH OSBP sequence was obtained from GFP-PH OSBP (Tim Levine, UCL Institute of Ophthalmology). iRFP-P4C was cloned amplifying the iRFP coding sequence piRFP670-N1 (Addgene plasmid # 45457) and inserted at AgeI and BsrGI cloning sites in GFP-P4C SidC (kind gift of Yuxin Mao, Cornell).

    Techniques:

    TagRFP-T-VAPB (1-218) -eMagB is shown on the left, iRFP-P4C is shown on the right. Scale bar: 5 μm.

    Journal: eLife

    Article Title: Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells

    doi: 10.7554/eLife.63230

    Figure Lengend Snippet: TagRFP-T-VAPB (1-218) -eMagB is shown on the left, iRFP-P4C is shown on the right. Scale bar: 5 μm.

    Article Snippet: PH OSBP sequence was obtained from GFP-PH OSBP (Tim Levine, UCL Institute of Ophthalmology). iRFP-P4C was cloned amplifying the iRFP coding sequence piRFP670-N1 (Addgene plasmid # 45457) and inserted at AgeI and BsrGI cloning sites in GFP-P4C SidC (kind gift of Yuxin Mao, Cornell).

    Techniques:

    TagRFP-T-VAPB (1-218) -eMagB is shown on the left, iRFP-P4C is shown on the right. Scale bar: 5 μm.

    Journal: eLife

    Article Title: Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells

    doi: 10.7554/eLife.63230

    Figure Lengend Snippet: TagRFP-T-VAPB (1-218) -eMagB is shown on the left, iRFP-P4C is shown on the right. Scale bar: 5 μm.

    Article Snippet: PH OSBP sequence was obtained from GFP-PH OSBP (Tim Levine, UCL Institute of Ophthalmology). iRFP-P4C was cloned amplifying the iRFP coding sequence piRFP670-N1 (Addgene plasmid # 45457) and inserted at AgeI and BsrGI cloning sites in GFP-P4C SidC (kind gift of Yuxin Mao, Cornell).

    Techniques:

    Journal: eLife

    Article Title: Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells

    doi: 10.7554/eLife.63230

    Figure Lengend Snippet:

    Article Snippet: PH OSBP sequence was obtained from GFP-PH OSBP (Tim Levine, UCL Institute of Ophthalmology). iRFP-P4C was cloned amplifying the iRFP coding sequence piRFP670-N1 (Addgene plasmid # 45457) and inserted at AgeI and BsrGI cloning sites in GFP-P4C SidC (kind gift of Yuxin Mao, Cornell).

    Techniques: Sequencing, Recombinant, Cloning, Generated, Software