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ipf lung fibroblasts  (Lonza)


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    Lonza ipf lung fibroblasts
    Ipf Lung Fibroblasts, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ipf+fibroblasts/ipf+lung+fibroblasts/10__1042_slash_bcj20253033-273-5-8
    Average 90 stars, based on 1 article reviews
    ipf lung fibroblasts - by Bioz Stars, 2026-09
    90/100 stars

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    Recombinant:

    Article Title: Extracellular Mitochondrial DNA Is Generated by Fibroblasts and Predicts Death in Idiopathic Pulmonary Fibrosis
    Article Snippet: .. NHLFs and IPF fibroblasts obtained from Lonza (Lonza, Allendale, NJ) and Asterand (Asterand Bioscience, Detroit, MI) were used between passages 3-7 and stimulated with and without 5 ng/ml of recombinant human TGFβ1 as previously described [14]. .. Cellular metabolism was measured with the Seahorse XFe96 Extracellular Flux Analyzer (Seahorse Bioscience, North Billerica, MA) for real-time analysis of extracellular acidification rate (ECAR) and oxygen consumption rate (OCR).

    Article Title: Extracellular Mitochondrial DNA Is Generated by Fibroblasts and Predicts Death in Idiopathic Pulmonary Fibrosis
    Article Snippet: .. NHLFs and IPF fibroblasts obtained from Lonza (Lonza, Allendale, NJ) and Asterand (Asterand Bioscience, Detroit, MI) were cultured in DMEM/2%FBS/1%Pen-Strep, used between passages 3-7, and stimulated with and without 2 ng/ml or 5 ng/ml of recombinant human TGFβ1 for 24 hours or 7 days as previously described [3]. .. Cellular metabolism was measured with the Seahorse XFe96 Extracellular Flux Analyzer (Seahorse Bioscience, North Billerica, MA) for real-time analysis of extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) according to the manufacturer’s protocol.

    Cell Culture:

    Article Title: mTOR Signaling Regulates Multiple Metabolic Pathways in Human Lung Fibroblasts After TGF-β and in Pulmonary Fibrosis
    Article Snippet: .. Normal human lung fibroblasts (HLFs) and IPF fibroblasts were purchased from Lonza and cultured as previously described ( 15 ). ..

    Article Title: Extracellular Mitochondrial DNA Is Generated by Fibroblasts and Predicts Death in Idiopathic Pulmonary Fibrosis
    Article Snippet: .. NHLFs and IPF fibroblasts obtained from Lonza (Lonza, Allendale, NJ) and Asterand (Asterand Bioscience, Detroit, MI) were cultured in DMEM/2%FBS/1%Pen-Strep, used between passages 3-7, and stimulated with and without 2 ng/ml or 5 ng/ml of recombinant human TGFβ1 for 24 hours or 7 days as previously described [3]. .. Cellular metabolism was measured with the Seahorse XFe96 Extracellular Flux Analyzer (Seahorse Bioscience, North Billerica, MA) for real-time analysis of extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) according to the manufacturer’s protocol.

    other:

    Article Title: Antifibrotics Modify B-Cell–induced Fibroblast Migration and Activation in Patients with Idiopathic Pulmonary Fibrosis
    Article Snippet: Normal fibroblasts and IPF fibroblasts were obtained from Lonza (Lonza Bioscience).



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    Relative mRNA expression levels of Axl, Tyro3, and Gas6 genes in IPF FBs and HPFs quantified by RT-qPCR. No Mer expression was detected in both fibroblast types. TBP gene was used as housekeeping gene. N = 4.

    Journal: Medicina

    Article Title: Evaluation of TAM Receptor Targeting in Pathophysiology of Idiopathic Pulmonary Fibrosis

    doi: 10.3390/medicina61101837

    Figure Lengend Snippet: Relative mRNA expression levels of Axl, Tyro3, and Gas6 genes in IPF FBs and HPFs quantified by RT-qPCR. No Mer expression was detected in both fibroblast types. TBP gene was used as housekeeping gene. N = 4.

    Article Snippet: IPF fibroblasts (IPF FBs CCL-134, ATCC) were cultured in Ham’s F12K medium supplemented with 10% FBS and 1% penicillin/streptomycin solution, and cells were used between passage 11 and 24.

    Techniques: Expressing, Quantitative RT-PCR