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ip3r antagonist 2 apb  (MedChemExpress)


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    Structured Review

    MedChemExpress ip3r antagonist 2 apb
    Ip3r Antagonist 2 Apb, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ip3r+antagonist/IP3+Receptor+Antibody/pm41833704-84-16-21
    Average 94 stars, based on 5 article reviews
    ip3r antagonist 2 apb - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Plasmid Preparation:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Expressing:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Labeling:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Imaging:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Fluorescence:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Two Tailed Test:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Blocking Assay:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Migration:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Gene Expression:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Quantitative RT-PCR:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Transwell Assay:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Wound Healing Assay:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Inhibition:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Transduction:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Translocation Assay:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Cell Culture:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Immunofluorescence:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Dominant Negative Mutation:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Western Blot:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Control:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Construct:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    shRNA:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Recombinant:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Sequencing:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Activity Assay:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Software:

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTAAM (HY100545), a PKC inhibitor Midostaurin (HY10230), and an IP3R antagonist (HYW009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).TAK1 inhibitors 5Z-7-oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma-Aldrich (St. Louis, MO, USA).. An intracellular calcium chelator BAPTA-AM (HY-100545), a PKC inhibitor Midostaurin (HY-10230), and an IP3R antagonist (HY-W009724) were obtained from MedChemExpress (NJ, USA).. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: inhibitors 5Z- 7- oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from Sigma- Aldrich (St. Louis, MO, USA). .. An intracellular calcium chelator BAPTA- AM (HY- 100545), a PKC inhibitor Midostaurin (HY- 10230), and an IP3R antagonist (HY- W009724) were obtained from MedChemExpress (NJ, USA). .. EGF Recombinant Human Protein (#PHG0311) was purchased from Gibco (Carlsbad, CA, USA).



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    Both Ca 2+ influx and Ca 2+ release from intracellular stores contribute to the ALI-induced [Ca 2+ ] i increases. (Aa,Ab) Typical traces showing switching from normal HBSS to 0 Ca 2+ HBSS (Aa) or depletion of intracellular Ca 2+ stores with thapsigargin ( Ab , Tg, 2 μM) reduced the ALI-evoked [Ca 2+ ] i increase. Note that thapsigargin alone induced a sustained increase of [Ca 2+ ] i . (Ac,Ad) Summary data for 0 Ca 2+ HBSS ( Ac , n = 496 cells, p < 0.01); and thapsigargin ( Ad , n = 352 cells, p < 0.001). (Ba–Bc) Typical tracings showing that Xest-C (5 μM), the IP3 receptor-specific antagonist (Ba) , ryanodine (100 μM), the ryanodine receptor antagonist (Bb) , and BAPTA-AM (2 μM, Bc ) reduced the ALI-induced [Ca 2+ ] i increase. (Bd–Bf) Summary data for Xest-C ( Bd , n = 224 cells, p < 0.001), ryanodine ( Be , n = 401 cells, p < 0.01), and BAPTA-AM ( Bf , n = 206 cells, p < 0.001).

    Journal: Frontiers in Physiology

    Article Title: Cell Deformation at the Air-Liquid Interface Evokes Intracellular Ca 2+ Increase and ATP Release in Cultured Rat Urothelial Cells

    doi: 10.3389/fphys.2021.631022

    Figure Lengend Snippet: Both Ca 2+ influx and Ca 2+ release from intracellular stores contribute to the ALI-induced [Ca 2+ ] i increases. (Aa,Ab) Typical traces showing switching from normal HBSS to 0 Ca 2+ HBSS (Aa) or depletion of intracellular Ca 2+ stores with thapsigargin ( Ab , Tg, 2 μM) reduced the ALI-evoked [Ca 2+ ] i increase. Note that thapsigargin alone induced a sustained increase of [Ca 2+ ] i . (Ac,Ad) Summary data for 0 Ca 2+ HBSS ( Ac , n = 496 cells, p < 0.01); and thapsigargin ( Ad , n = 352 cells, p < 0.001). (Ba–Bc) Typical tracings showing that Xest-C (5 μM), the IP3 receptor-specific antagonist (Ba) , ryanodine (100 μM), the ryanodine receptor antagonist (Bb) , and BAPTA-AM (2 μM, Bc ) reduced the ALI-induced [Ca 2+ ] i increase. (Bd–Bf) Summary data for Xest-C ( Bd , n = 224 cells, p < 0.001), ryanodine ( Be , n = 401 cells, p < 0.01), and BAPTA-AM ( Bf , n = 206 cells, p < 0.001).

    Article Snippet: To determine whether these two receptors contribute to the ALI-induced [Ca 2+ ] i increase, xestospongin-C (Xest-C; ; Sigma), the IP3R specific antagonist, or ryanodine (MCE, China), the RyR antagonist, were applied between the two ALI stimuli and during the second ALI stimulus.

    Techniques:

    Expression of markers involved in Ca 2+ signaling in RYR2 –/– -iPSC-CMs compared to Ctrl-iPSC-CMs. (A) Reverse transcription-PCR analyses showing mRNA expression of transcripts for Ca 2+ handling-related genes ( RYR2, IP3R1, IP3R2 , and CACNA1C ), genes encoding sarcomeric proteins ( TNNT2 and ACTN2 ), and the housekeeping gene GAPDH in Ctrl- and A3 RYR2 –/– -iPSC-CMs (Ctrl: n = 6 and RYR2 –/– -A3: n = 6 different differentiation experiments). M: DNA molecular-weight size marker. (B) Western blot showing expression of Ca 2+ handling-associated proteins (IP3R, SERCA2A, and NCX1) in both Ctrl- and A3 RYR2 –/– -iPSC-CMs. (C–E) Scatter dot plot showing protein levels of IP3R (C) , SERCA2A (D) , and NCX1 (E) normalized to GAPDH between Ctrl- and A3 RYR2 –/– -iPSC-CMs (Ctrl: n = 4 and A3 RYR2 –/– : n = 5 different differentiation experiments). (F) Representative immunostaining of Ctrl- and RYR2 –/– -iPSC-CMs for IP3R (green) and α-actinin (red). Cells were counterstained with DAPI (blue). Scale bar, 100 μm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: IP3R-Mediated Compensatory Mechanism for Calcium Handling in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes With Cardiac Ryanodine Receptor Deficiency

    doi: 10.3389/fcell.2020.00772

    Figure Lengend Snippet: Expression of markers involved in Ca 2+ signaling in RYR2 –/– -iPSC-CMs compared to Ctrl-iPSC-CMs. (A) Reverse transcription-PCR analyses showing mRNA expression of transcripts for Ca 2+ handling-related genes ( RYR2, IP3R1, IP3R2 , and CACNA1C ), genes encoding sarcomeric proteins ( TNNT2 and ACTN2 ), and the housekeeping gene GAPDH in Ctrl- and A3 RYR2 –/– -iPSC-CMs (Ctrl: n = 6 and RYR2 –/– -A3: n = 6 different differentiation experiments). M: DNA molecular-weight size marker. (B) Western blot showing expression of Ca 2+ handling-associated proteins (IP3R, SERCA2A, and NCX1) in both Ctrl- and A3 RYR2 –/– -iPSC-CMs. (C–E) Scatter dot plot showing protein levels of IP3R (C) , SERCA2A (D) , and NCX1 (E) normalized to GAPDH between Ctrl- and A3 RYR2 –/– -iPSC-CMs (Ctrl: n = 4 and A3 RYR2 –/– : n = 5 different differentiation experiments). (F) Representative immunostaining of Ctrl- and RYR2 –/– -iPSC-CMs for IP3R (green) and α-actinin (red). Cells were counterstained with DAPI (blue). Scale bar, 100 μm.

    Article Snippet: To investigate the role of IP3R-mediated Ca 2+ release in Ca 2+ handling of iPSC-CMs, spontaneous Ca 2+ transients were recorded before and after the application of the IP3R antagonists 2-aminoethoxydiphenyl borate (2-APB, 20 μM; Tocris) and Xestospongin C (XeC, 1 μM; abcam).

    Techniques: Expressing, Reverse Transcription, Molecular Weight, Marker, Western Blot, Immunostaining

    Contributions of IP3R-mediated Ca 2+ release to spontaneous Ca 2+ transients in Ctrl- and RYR2 –/– -iPSC-CMs. (A,B) Representative cytosolic Ca 2+ dynamics in Ctrl- (A) and RYR2 –/– -iPSC-CMs (B) before and after the addition of IP3R antagonist 2-APB (20 μM), and after the washout of the drug. (C) Pie charts depict the percentage of cells maintaining Ca 2+ transients after the treatment with 2-APB for 500 s. (D,E) Changes of Ca 2+ transient amplitude (D) and frequency (E) after the treatment and the following removal of 2-APB, which were normalized to those from the same cell under the basal condition (Ctrl: n = 13 cells from three differentiation experiments; A3 RYR2 –/– : n = 14 cells from three differentiation experiments). (F,G) Representative cytosolic Ca 2+ dynamics in Ctrl- (F) and RYR2 –/– -iPSC-CMs (G) before and after the addition of IP3R inhibitor Xestospongin C (XeC;1 μM), and after the washout of the drug. (H) Pie charts depict the percentage of cells maintaining Ca 2+ transients after the treatment with XeC for 200 s. (I,J) Changes of Ca 2+ transient amplitude (I) and frequency (J) after the treatment and following the washout of XeC, which were normalized to those from the same cell under the basal condition (Ctrl: n = 14 cells from three differentiation experiments; A3 RYR2 –/– : n = 13 cells from three differentiation experiments; A5 RYR2 –/– : n = 10 cells from two differentiation experiments). * P < 0.05; ** P < 0.01; *** P < 0.001;**** P < 0.0001 by using the one-way ANOVA with the Dunnett’s multiple comparison test.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: IP3R-Mediated Compensatory Mechanism for Calcium Handling in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes With Cardiac Ryanodine Receptor Deficiency

    doi: 10.3389/fcell.2020.00772

    Figure Lengend Snippet: Contributions of IP3R-mediated Ca 2+ release to spontaneous Ca 2+ transients in Ctrl- and RYR2 –/– -iPSC-CMs. (A,B) Representative cytosolic Ca 2+ dynamics in Ctrl- (A) and RYR2 –/– -iPSC-CMs (B) before and after the addition of IP3R antagonist 2-APB (20 μM), and after the washout of the drug. (C) Pie charts depict the percentage of cells maintaining Ca 2+ transients after the treatment with 2-APB for 500 s. (D,E) Changes of Ca 2+ transient amplitude (D) and frequency (E) after the treatment and the following removal of 2-APB, which were normalized to those from the same cell under the basal condition (Ctrl: n = 13 cells from three differentiation experiments; A3 RYR2 –/– : n = 14 cells from three differentiation experiments). (F,G) Representative cytosolic Ca 2+ dynamics in Ctrl- (F) and RYR2 –/– -iPSC-CMs (G) before and after the addition of IP3R inhibitor Xestospongin C (XeC;1 μM), and after the washout of the drug. (H) Pie charts depict the percentage of cells maintaining Ca 2+ transients after the treatment with XeC for 200 s. (I,J) Changes of Ca 2+ transient amplitude (I) and frequency (J) after the treatment and following the washout of XeC, which were normalized to those from the same cell under the basal condition (Ctrl: n = 14 cells from three differentiation experiments; A3 RYR2 –/– : n = 13 cells from three differentiation experiments; A5 RYR2 –/– : n = 10 cells from two differentiation experiments). * P < 0.05; ** P < 0.01; *** P < 0.001;**** P < 0.0001 by using the one-way ANOVA with the Dunnett’s multiple comparison test.

    Article Snippet: To investigate the role of IP3R-mediated Ca 2+ release in Ca 2+ handling of iPSC-CMs, spontaneous Ca 2+ transients were recorded before and after the application of the IP3R antagonists 2-aminoethoxydiphenyl borate (2-APB, 20 μM; Tocris) and Xestospongin C (XeC, 1 μM; abcam).

    Techniques: Comparison