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Bio X Cell buffer
Buffer, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/invivopure/InVivoPure+pH+7%2E0+Dilution+Buffer/pmc13584615-77-7-8
Average 96 stars, based on 144 article reviews

buffer - by Bioz Stars, 2026-10

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Bioprocessing:

Article Title: Microglia-independent rAAV-induced inflammation causes persistent ocular immune dysregulation rescued by S1P receptor modulation.
Article Snippet: Cyclosporine A [20 mg/kg] (CAS 59865-13-3; HY-B0579; 629 MedChemExpress, Monmouth Junction, NJ, USA) and tacrolimus [3mg/kg] (CAS 104987-11-3; 630 HY-13756A; MedChemExpress) formulated in PBS with 5% DMSO and 0.1% Tween-20 (BP337-631 100; ThermoFisher Scientific) were both administered i.p. every day from -7 to 28 dpi.111,112 632 Pioglitazone [5.3mg/kg] (CAS 111025-46-8, B1117; APExBIO Technology, TX, USA) and 633 Jo urn al Pr -pr oo f 28 fenofibrate [20 mg/kg] (CAS 49562-28-9; F6020; Merck, Darmstadt, Germany) formulated in 5% 634 DMSO, 40% PEG300 (90878, Sigma-Aldrich), 5% Tween-80 (CAS 9005-65-6, 11441981, Fisher 635 Scientific) and 50% saline (0.9% NaCl w/v in water) were administered i.p. from -1 to 28 dpi.51,52 636 Control groups received corresponding vehicle formulation. .. 637 Methotrexate (CAS 59-05-2; NDC 0703-3671-01; Teva Pharmaceuticals, Parsippany, NJ, USA) 638 and Mycophenolate mofetil (CAS 116680-01-4; NDC 17478-422-40; Akorn) were delivered ad 639 libitum supplemented in drinking water at 6.25 μg/mL and 0.625 mg/ml from -28 to 28 dpi as 640 previously described.71 For monoclonal antibodies: Anti-mouse CD20 [20mg/kg] (clone MB20-641 11; BE0356; BioXCell, Lebanon, NH, USA) was administered i.p. weekly from -7 to 28 dpi;113 Anti-642 mouse TNFα [10 mg/kg] (clone XT3.11; BE0058; BioXCell) was administered i.p. from -1, 0, and 1 643 dpi, then alternate days until 28 dpi.114 Mouse anti-dengue virus IgG2c isotype control (BE0366; 644 BioXCell) formulated in InVivoPure pH 7.0 Dilution Buffer (IP0070; BioXCell) for anti-CD20 or anti-645 TNFα administered following each regimen. .. Anti-mouse IL-6 [1mg/kg] (clone MP5-20F3; 16-646 7061-81; ThermoFisher Scientific) formulated in PBS was administered i.p. on -1, 1, 3, and 7 647 dpi.115 648 649 Flow Cytometry 650 According to our established method, retinae and vitreous humor were harvested by 651 ocular dissection in 100 μL ice-cold PBS (D8537; Sigma-Aldrich) via limbal incision, lens and 652 anterior chamber removal, and transfer into a 1.5 mL microcentrifuge tube.5,36 Subsequently, 653 samples were processed into single-cell suspensions by mechanical disruption and filtered 654 through a 60 μm nylon mesh filter plate (MANMN6010; Sigma-Aldrich) centrifuged at 400 × g for 655 5 minutes, with the supernatant aspirated and cell pellet resuspended in Cell Staining Buffer 656 (420201; BioLegend) prior to FC receptor inhibition (20 minutes at 4°C) and staining with 657 fluorophore-conjugated monoclonal antibodies and viability dye (30 minutes at 4°C) (Table S30).

Virus:

Control:

Article Title: Identification of endometrial CD169 + macrophages essential for Treg cell accumulation and implantation.
Article Snippet: .. In vivo depletion of CD169+ macrophages For CSF1-dependent depletion, C57BL/6 WT females were treated with a CSF1-neutralizing antibody (Bio X Cell; Cat. No. BE0204; 100μg/mouse, i.p.) or same amount of isotype control (Bio X Cell; Cat. No. BE0088; 100μg/mouse, i.p.) diluted in 150ul of InVivoPure pH 8.0 Dilution Buffer (Bio X Cell; Cat. No. IP0080) on days − 8, − 6 and − 4 prior to analysis. .. To selectively ablate CD169+ macrophages, CD169-DTR mouse received 0.5 μg of diphtheria toxin (FUJIFILM; Cat. No. 048–34371) in 100μL saline via intraperitoneal injection (i.p.).

Article Title: Ly6G + granulocytes-derived IL-17 limits protective host responses and promotes tuberculosis pathogenesis
Article Snippet: Fixable Viability Dye eFluor 780 used for live/dead staining was obtained from eBioscience. .. InVivoMAb anti-mouse IL-17A (BE0173), InVivoPure pH 7.0 Dilution Buffer (IP0070), InVivoMAb mouse IgG1 isotype control, unknown specificity (BE0083), InVivoPure pH 6.5 Dilution Buffer (IP0065) were purchased from BioXcell. .. Mouse IL-17A ELISA Kit (ab199081), mouse PGE2 ELISA Kit ab287802 and Cytokine & Chemokine 36-Plex Mouse ProcartaPlexTM Panel 1 A (EPX360-26092-901) for Luminex assay were obtained from Abcam and Thermo Fisher, respectively.

In Vivo:

Article Title: Anti-CSF-1R therapy with combined immuno-chemotherapy coordinate an adaptive immune response to eliminate macrophage enriched triple negative breast cancers.
Article Snippet: For ICB treatment, mice were administered RecombiMab anti-mouse PD1 (BioXcell, CD279, CP151) and RecombiMAb anti-mouse IgG2a isotype (BioXcell, CP150) 200 μg per mouse every three days via i.p. injection. .. In vivo antibodies were diluted with InVivoPure pH 7.0 Dilution Buffer (BioXCell, IP0070). ..

Article Title: Anti-CSF-1R therapy with combined immuno-chemotherapy coordinate an adaptive immune response to eliminate macrophage enriched triple negative breast cancers
Article Snippet: For ICB treatment, mice were administered RecombiMab anti-mouse PD1 (BioXcell, CD279, CP151) and RecombiMAb anti-mouse IgG2a isotype (BioXcell, CP150) 200 μg per mouse every 3 days via i.p. injection. .. In vivo antibodies were diluted with InVivoPure pH 7.0 Dilution Buffer (BioXCell, IP0070). ..

Injection:

Article Title: An immunometabolic prodrug strategy overcomes DHODH inhibitor resistance in refractory melanoma.
Article Snippet: .. In the experiment of combined use of immune checkpoint inhibitors, anti-mouse PD − 1 (BioXCell, BE0273, RRID: AB_2687796) was diluted to 200 μg with InVivoPure pH 7.0 dilution buffer, and each mouse was intraperitoneally injected with 200 μg of PD − 1 on days 7, 10, 13, and 16 for treatment. .. The data analysis were performed using GraphPad prism 9.5.0 software and results were presented as mean ± standard deviation (SD).

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Experimental Design Ldlr −/− mice were transplanted with a mixture of bone marrow (BM) from Mx1-Cre-Jak2 VF (20%) and wild-type mice (80%) to generate a Jak2 VF clonal hematopoiesis (CH) model or with BM from Mx1-Cre (20%) and wild-type mice as controls. At 4 weeks (wks) after bone marrow transplantation (BMT), mice received 2 pIpC (100 μg per dose) injections to induce Cre . Two wks later, mice were fed a Western-type diet (WTD) for a period of 8 and 10 wks or 16 wks. At 4 wks of WTD feeding, mice were injected with IgG or anti–IL-18 for a period of 4, 6, or 12 wks, respectively. The graphical illustrations were created with BioRender.com.

Journal: JACC: Basic to Translational Science

Article Title: Interleukin-18 Inhibition Aggravates Atherosclerosis in Jak2 V617F Clonal Hematopoiesis

doi: 10.1016/j.jacbts.2025.101463

Figure Lengend Snippet: Experimental Design Ldlr −/− mice were transplanted with a mixture of bone marrow (BM) from Mx1-Cre-Jak2 VF (20%) and wild-type mice (80%) to generate a Jak2 VF clonal hematopoiesis (CH) model or with BM from Mx1-Cre (20%) and wild-type mice as controls. At 4 weeks (wks) after bone marrow transplantation (BMT), mice received 2 pIpC (100 μg per dose) injections to induce Cre . Two wks later, mice were fed a Western-type diet (WTD) for a period of 8 and 10 wks or 16 wks. At 4 wks of WTD feeding, mice were injected with IgG or anti–IL-18 for a period of 4, 6, or 12 wks, respectively. The graphical illustrations were created with BioRender.com.

Article Snippet: At 4 weeks after WTD feeding, mice were injected intraperitoneally with IL-18 antibodies (Bio X Cell, BE0237); 500 μg dissolved in 100 μL of in vivo dilution buffer (Bio X Cell, IP0070) or IgG isotype control (Bio X Cell, BE0089); 500 μg dissolved in 100 μL of in vivo dilution buffer (Bio X Cell, IP0070) 3 times per week with a 55- to 60-hour interval between injections.

Techniques: Transplantation Assay, Western Blot, Injection

The Impact of IL-18 Antibody Treatment on Lesion Area and Necrotic Core Formation in Jak2 VF CH Mice (A) Representative hematoxylin and eosin (H&E) images of aortic root lesions. Necrotic cores are marked by dashed lines. Scale bar, 200 μm. (B) Quantification of lesion area and (C) necrotic core area. (D) Number of foam cell rich lesions, collagen rich lesions, and advanced lesions with necrotic cores per group based on H and E staining and expressed per number of total observations. For B to D, n = 12-15 mice for 8-wk, n = 14 for 10-wk, and n = 10-14 for the 16-wk cohort. For B and C, statistical analysis was performed using 2-way analysis of variance with Tukey’s multiple comparison test and for D using the chi-square test. Statistical differences are indicated on the graphs, with P values >0.05 omitted. Statistically significant differences by treatment and genotype factors are presented below the graphs. Abbreviations as in .

Journal: JACC: Basic to Translational Science

Article Title: Interleukin-18 Inhibition Aggravates Atherosclerosis in Jak2 V617F Clonal Hematopoiesis

doi: 10.1016/j.jacbts.2025.101463

Figure Lengend Snippet: The Impact of IL-18 Antibody Treatment on Lesion Area and Necrotic Core Formation in Jak2 VF CH Mice (A) Representative hematoxylin and eosin (H&E) images of aortic root lesions. Necrotic cores are marked by dashed lines. Scale bar, 200 μm. (B) Quantification of lesion area and (C) necrotic core area. (D) Number of foam cell rich lesions, collagen rich lesions, and advanced lesions with necrotic cores per group based on H and E staining and expressed per number of total observations. For B to D, n = 12-15 mice for 8-wk, n = 14 for 10-wk, and n = 10-14 for the 16-wk cohort. For B and C, statistical analysis was performed using 2-way analysis of variance with Tukey’s multiple comparison test and for D using the chi-square test. Statistical differences are indicated on the graphs, with P values >0.05 omitted. Statistically significant differences by treatment and genotype factors are presented below the graphs. Abbreviations as in .

Techniques: Staining, Comparison

IL-18 Antibody Treatment Enhances Fibrous Cap Thickness and Collagen Content in Atherosclerotic Lesions (A) Representative images of Masson’s Trichrome staining showing collagen staining in lesions. Black bars indicate cap thickness. Scale bar, 200 μm. (B) Total collagen staining (blue) in lesions and (C) Quantification of fibrous cap thickness (n = 12-15 mice for 8-wk, n = 14 for 10-wk, and n = 10-14 for 16-wk cohort) at 8, 10, and 16 wks WTD feeding. For B and C statistical analysis was performed using 2-way analysis of variance with Tukey’s multiple comparison test. Statistical differences are indicated on the graphs, with P values >0.05 omitted. Statistically significant differences by treatment and genotype factors are presented below the graphs. Abbreviations as in .

Journal: JACC: Basic to Translational Science

Article Title: Interleukin-18 Inhibition Aggravates Atherosclerosis in Jak2 V617F Clonal Hematopoiesis

doi: 10.1016/j.jacbts.2025.101463

Figure Lengend Snippet: IL-18 Antibody Treatment Enhances Fibrous Cap Thickness and Collagen Content in Atherosclerotic Lesions (A) Representative images of Masson’s Trichrome staining showing collagen staining in lesions. Black bars indicate cap thickness. Scale bar, 200 μm. (B) Total collagen staining (blue) in lesions and (C) Quantification of fibrous cap thickness (n = 12-15 mice for 8-wk, n = 14 for 10-wk, and n = 10-14 for 16-wk cohort) at 8, 10, and 16 wks WTD feeding. For B and C statistical analysis was performed using 2-way analysis of variance with Tukey’s multiple comparison test. Statistical differences are indicated on the graphs, with P values >0.05 omitted. Statistically significant differences by treatment and genotype factors are presented below the graphs. Abbreviations as in .

Techniques: Staining, Comparison

IL-18 Antibody Treatment Decreased Cl-GSDMD levels, IFN-γ, and AIM2 expression in Atherosclerotic Lesions of Jak2 VF CH Mice (A and B) IFN-γ immunostaining. (A) Representative images. Interferon (IFN)-γ is shown in green. (B) Quantification was performed as mean fluorescence intensity (MFI) (n = 12-15 mice for 8-wk, n = 12 for 10-wk, and n = 10 for 16-wk cohort). (C-D) AIM2 inflammasome expression in macrophages in lesions. (C) Representative IF images of aortic root sections stained for AIM2 (magenta) and Mac2 (green). Arrows indicate macrophages positive for AIM2. Quantification was performed as MFI of AIM2 signal within Mac2 + macrophages using ImageJ (D) quantification, Mac2 + areas were thresholded to create a binary mask, which was applied to the AIM2 channel, and the mean gray value of AIM2 signal within Mac2 + regions was measured (n = 12 mice for 10-wk and n = 8-14 for 16-wk cohort). (E and F) Cleaved Gasdermin D (Cl-GSDMD) expression in macrophages in the lesions. (E) Representative images. Cl-GSDMD is shown in magenta. Cl-GSDMD–positive macrophages (white spots) highlighted by yellow arrows in lesions (F) Quantification (n = 15 mice for 10-wk and n = 10 for 16-wk cohort). Scale bar for all images, 100 μm. For B, D, and F, statistical analysis was performed using 2-way analysis of variance with Tukey’s multiple comparison test. Abbreviations as in .

Journal: JACC: Basic to Translational Science

Article Title: Interleukin-18 Inhibition Aggravates Atherosclerosis in Jak2 V617F Clonal Hematopoiesis

doi: 10.1016/j.jacbts.2025.101463

Figure Lengend Snippet: IL-18 Antibody Treatment Decreased Cl-GSDMD levels, IFN-γ, and AIM2 expression in Atherosclerotic Lesions of Jak2 VF CH Mice (A and B) IFN-γ immunostaining. (A) Representative images. Interferon (IFN)-γ is shown in green. (B) Quantification was performed as mean fluorescence intensity (MFI) (n = 12-15 mice for 8-wk, n = 12 for 10-wk, and n = 10 for 16-wk cohort). (C-D) AIM2 inflammasome expression in macrophages in lesions. (C) Representative IF images of aortic root sections stained for AIM2 (magenta) and Mac2 (green). Arrows indicate macrophages positive for AIM2. Quantification was performed as MFI of AIM2 signal within Mac2 + macrophages using ImageJ (D) quantification, Mac2 + areas were thresholded to create a binary mask, which was applied to the AIM2 channel, and the mean gray value of AIM2 signal within Mac2 + regions was measured (n = 12 mice for 10-wk and n = 8-14 for 16-wk cohort). (E and F) Cleaved Gasdermin D (Cl-GSDMD) expression in macrophages in the lesions. (E) Representative images. Cl-GSDMD is shown in magenta. Cl-GSDMD–positive macrophages (white spots) highlighted by yellow arrows in lesions (F) Quantification (n = 15 mice for 10-wk and n = 10 for 16-wk cohort). Scale bar for all images, 100 μm. For B, D, and F, statistical analysis was performed using 2-way analysis of variance with Tukey’s multiple comparison test. Abbreviations as in .

Techniques: Expressing, Immunostaining, Fluorescence, Staining, Comparison

IL-18 Antibody Treatment Increases Apoptotic Macrophages and Impairs Efferocytosis (A and B) Apoptotic cleaved (Cl)-Caspase-3 + macrophages. (A) Representative images. Cl-caspase-3 is shown in magenta. Cl-caspase-3 positive macrophages (white spots) highlighted by yellow arrows in lesions. (B) Quantification (n = 13-15 mice for 10-wk and n = 7-10 for 16-wk cohort). (C and D) TUNEL assay. (C) TUNEL + staining visualized in red spots; dead macrophages (yellow spots) indicated by yellow arrows. (D) Quantification (n = 15 mice per group for 10-wk and n = 10 for 16-wk cohort). (E-F) GSDME, a pyroptotic marker, in macrophages. (E) Representative images. GSDME is shown in magenta. GSDME-positive macrophages (white spots) highlighted by yellow arrows in lesions. (F) Quantification (n = 15 mice per group for 10-wk and n = 10 for 16-wk cohort). (G) Representative images and in situ efferocytosis assessment (n = 15 mice per group for 10-wk and n = 10 for 16-wk cohort). Scale bar for all images, 100 μm. For B, D, and F, statistical analysis was performed using 2-way analysis of variance with Tukey’s multiple comparison test. Abbreviations as in .

Journal: JACC: Basic to Translational Science

Article Title: Interleukin-18 Inhibition Aggravates Atherosclerosis in Jak2 V617F Clonal Hematopoiesis

doi: 10.1016/j.jacbts.2025.101463

Figure Lengend Snippet: IL-18 Antibody Treatment Increases Apoptotic Macrophages and Impairs Efferocytosis (A and B) Apoptotic cleaved (Cl)-Caspase-3 + macrophages. (A) Representative images. Cl-caspase-3 is shown in magenta. Cl-caspase-3 positive macrophages (white spots) highlighted by yellow arrows in lesions. (B) Quantification (n = 13-15 mice for 10-wk and n = 7-10 for 16-wk cohort). (C and D) TUNEL assay. (C) TUNEL + staining visualized in red spots; dead macrophages (yellow spots) indicated by yellow arrows. (D) Quantification (n = 15 mice per group for 10-wk and n = 10 for 16-wk cohort). (E-F) GSDME, a pyroptotic marker, in macrophages. (E) Representative images. GSDME is shown in magenta. GSDME-positive macrophages (white spots) highlighted by yellow arrows in lesions. (F) Quantification (n = 15 mice per group for 10-wk and n = 10 for 16-wk cohort). (G) Representative images and in situ efferocytosis assessment (n = 15 mice per group for 10-wk and n = 10 for 16-wk cohort). Scale bar for all images, 100 μm. For B, D, and F, statistical analysis was performed using 2-way analysis of variance with Tukey’s multiple comparison test. Abbreviations as in .

Techniques: TUNEL Assay, Staining, Marker, In Situ, Comparison

Singe Cell RNA-Seq Analysis of Atherosclerotic Plaques Shows Reduced Expression of Efferocytosis Genes in Resident-Like Macrophages (A) UMAP of integrated scRNA-sequencing of CD45 + plaque cells isolated from aortic arches of control mice, Jak2 VF mice, and Jak2 VF mice with IL-18 antibodies. (B) Percentage of lesional CD45 + cells within each cluster. (C) Violin plots depicting gene expression levels of efferocytosis markers Axl , Mertk , and Cd36 in control, Jak2 VF , and Jak2 VF mice treated with IL-18 antibody, derived from scRNA-seq data. (D) Representative aortic images with DAPI, MAC2, and AXL staining. DAPI is shown in blue, MAC2 is shown in green, and AXL is shown in magenta. AXL-positive macrophages (white spots) are highlighted by yellow arrows in the lesions. (E) Quantification of lesional AXL positive macrophages (n = 15/group) after 10 weeks of WTD feeding. (F) Violin plots depicting gene expression levels of MHC II genes in Jak2 VF and Jak2 VF mice treated with IL-18 antibody, derived from scRNA-seq data. Scale bar, 100 μm. Statistical analyses were performed using the Wilcoxon rank sum test with Bonferroni correction in C and F. For E, statistical analysis was performed using 2-way analysis of variance with Tukey’s multiple comparison test. Statistical differences are indicated on the graphs, with P values >0.05 omitted. Statistically significant differences by treatment and genotype factors are presented below the graphs. Abbreviations as in .

Journal: JACC: Basic to Translational Science

Article Title: Interleukin-18 Inhibition Aggravates Atherosclerosis in Jak2 V617F Clonal Hematopoiesis

doi: 10.1016/j.jacbts.2025.101463

Figure Lengend Snippet: Singe Cell RNA-Seq Analysis of Atherosclerotic Plaques Shows Reduced Expression of Efferocytosis Genes in Resident-Like Macrophages (A) UMAP of integrated scRNA-sequencing of CD45 + plaque cells isolated from aortic arches of control mice, Jak2 VF mice, and Jak2 VF mice with IL-18 antibodies. (B) Percentage of lesional CD45 + cells within each cluster. (C) Violin plots depicting gene expression levels of efferocytosis markers Axl , Mertk , and Cd36 in control, Jak2 VF , and Jak2 VF mice treated with IL-18 antibody, derived from scRNA-seq data. (D) Representative aortic images with DAPI, MAC2, and AXL staining. DAPI is shown in blue, MAC2 is shown in green, and AXL is shown in magenta. AXL-positive macrophages (white spots) are highlighted by yellow arrows in the lesions. (E) Quantification of lesional AXL positive macrophages (n = 15/group) after 10 weeks of WTD feeding. (F) Violin plots depicting gene expression levels of MHC II genes in Jak2 VF and Jak2 VF mice treated with IL-18 antibody, derived from scRNA-seq data. Scale bar, 100 μm. Statistical analyses were performed using the Wilcoxon rank sum test with Bonferroni correction in C and F. For E, statistical analysis was performed using 2-way analysis of variance with Tukey’s multiple comparison test. Statistical differences are indicated on the graphs, with P values >0.05 omitted. Statistically significant differences by treatment and genotype factors are presented below the graphs. Abbreviations as in .

Techniques: RNA Sequencing, Expressing, Sequencing, Isolation, Control, Gene Expression, Derivative Assay, Staining, Comparison

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