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calf intestinal alkaline phosphatase  (Thermo Fisher)
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Thermo Fisher calf intestinal alkaline phosphatase
Calf Intestinal Alkaline Phosphatase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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intestinal permeability  (MedChemExpress)
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MedChemExpress intestinal permeability
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Intestinal Permeability, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human intestinal epithelial c2bbe1 cells  (ATCC)
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ATCC human intestinal epithelial c2bbe1 cells
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Human Intestinal Epithelial C2bbe1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse small intestine  (Jackson Laboratory)
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Jackson Laboratory mouse small intestine
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Mouse Small Intestine, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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experimental intestinal inflammation 496 c57bl 6j mice  (Charles River Laboratories)
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Charles River Laboratories experimental intestinal inflammation 496 c57bl 6j mice
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Experimental Intestinal Inflammation 496 C57bl 6j Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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heparin  (Thermo Fisher)
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Thermo Fisher heparin
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Heparin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Inserm Transfert human intestinal caco 2 cells
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Human Intestinal Caco 2 Cells, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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press cell culture intestinal epithelial cell line iec  (ATCC)
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ATCC press cell culture intestinal epithelial cell line iec
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Press Cell Culture Intestinal Epithelial Cell Line Iec, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human intestinal epithelial cell hiec 6  (ATCC)
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ATCC human intestinal epithelial cell hiec 6
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Human Intestinal Epithelial Cell Hiec 6, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thermosensitive alkaline phosphatase  (Thermo Fisher)
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Thermo Fisher thermosensitive alkaline phosphatase
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Thermosensitive Alkaline Phosphatase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on intestinal barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal permeability, as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.

Journal: Drug Design, Development and Therapy

Article Title: Probiotics in Combination with C16 Peptide and Angiopoietin-1 Synergistically Ameliorate Multiple Sclerosis in Mice

doi: 10.2147/DDDT.S614294

Figure Lengend Snippet: Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on intestinal barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal permeability, as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.

Article Snippet: Intestinal permeability was evaluated by measuring the flux of fluorescein isothiocyanate (FITC)-Dextran 4000 (4 kDa, CAS:60842–46-8, MedChemExpress).

Techniques: Probiotics, Immunofluorescence, Staining, Marker, Expressing, Control, Permeability