Journal: World Journal of Gastrointestinal Oncology

Article Title: Taurine suppresses gastric intestinal metaplasia in patient-derived organoids and Atp4a -/- mice

doi: 10.4251/wjgo.v18.i2.114161

Figure Lengend Snippet: Taurine increases the mucin 5AC (MUC5AC) level and inhibits the expression of caudal type homeobox 2, MUC2, and Trefoil factor family 3 in Atp4a -/- mice. A: Expression of caudal type homeobox 2 (CDX2), mucin 2 (MUC2), Trefoil factor family 3 (TFF3) and MUC5AC in Atp4a -/- mouse gastric tissues was assessed via immunohistochemistry (IHC) staining ( n = 6); B: IHC scores of CDX2, MUC2, and TFF3 ( n = 6); C: IHC score of MUC5AC ( n = 6). a P < 0.01 vs Atp4a -/- ; b P < 0.01 vs wild-type (WT).

Article Snippet: The gastric tissue sections were dewaxed, rehydrated, and incubated with primary antibodies against MUC5AC (1:200, GTX11335; GeneTex, Irvine, CA, United States), CDX2 (1:100, ab101532; Abcam, Cambridge, United Kingdom), MUC2 (1:2000, 27675-1-AP; Proteintech, Wuhan, China), and TFF3 (1:800, 23277-1-AP; Proteintech).

Techniques: Expressing, Immunohistochemistry

Journal: World Journal of Gastrointestinal Oncology

Article Title: Taurine suppresses gastric intestinal metaplasia in patient-derived organoids and Atp4a -/- mice

doi: 10.4251/wjgo.v18.i2.114161

Figure Lengend Snippet: Taurine downregulates the protein expression of caudal type homeobox 2 and Trefoil factor family 3 in gastric intestinal metaplasia control organoids. A-C: Western blotting was conducted to assess the protein levels of caudal type homeobox 2 (CDX2) and Trefoil factor family 3 (TFF3); D-I: Relative protein levels were quantified. a P < 0.01 vs normal control; b P < 0.05 vs gastric intestinal metaplasia (GIM) control (CON); c P < 0.01 vs GIM CON.

Article Snippet: The gastric tissue sections were dewaxed, rehydrated, and incubated with primary antibodies against MUC5AC (1:200, GTX11335; GeneTex, Irvine, CA, United States), CDX2 (1:100, ab101532; Abcam, Cambridge, United Kingdom), MUC2 (1:2000, 27675-1-AP; Proteintech, Wuhan, China), and TFF3 (1:800, 23277-1-AP; Proteintech).

Techniques: Expressing, Control, Western Blot

Journal: Endocrinology

Article Title: Differential recognition of a tyrosine-dependent signal in the basolateral and endocytic pathways of thyroid epithelial cells.

doi: 10.1210/endo.143.4.8734

Figure Lengend Snippet: FIG. 6. Double kinetics of internalization of endogenous TfR and transfected PLAP-PS321 mutant in FRT cells. After binding at 4 C with monoclonal antibody against TfR (A–C) and polyclonal antibody against PLAP (D–F), FRT cells expressing PLAP-PS321 were incu- bated at 37 C for 1, 10, and 20 min to allow internalization of the two receptors. Cells were then fixed and permeabilized. Both receptors were visualized in the same field using either antimouse rhodamine- labeled secondary antibody or antirabbit fluorescein-labeled second- ary antibody. Bar, 10 m. PLAP-PS321 is poorly internalized and never colocalizes with the intracellular staining of TfR but rather stays on the cell surface as small clusters of fluorescence.

Article Snippet: Antibodies were as follows: mouse monoclonal antibody ME 20.4 against the ectodomain of human neurotrophin re- ceptor, p75hNTR, monoclonal antibody antirat transferrin receptor Clone OX 26 (Crawley Down, Sussex, UK), polyclonal antibody against calf intestinal alkaline phosphatase (Rockland, Gilbertsville, PA), rabbit affinity purified antibody to whole molecule mouse IgG (Cappel, Organon Teknika Corp., West Chester, PA).

Techniques: Transfection, Mutagenesis, Binding Assay, Expressing, Labeling, Staining, Fluorescence