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il1β dy201  (R&D Systems)


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    Structured Review

    R&D Systems il1β dy201
    Il1β Dy201, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 569 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il1%CE%B2/Human+IL-1+beta%2FIL-1F2+DuoSet+ELISA/pm42009234-911-7-9
    Average 96 stars, based on 569 article reviews
    il1β dy201 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    In Vitro:

    Article Title: Inflammatory Mediators Both Directly and Indirectly Promote Microglial Proliferation.
    Article Snippet: .. After 5 days in vitro, we treated microglia with the following factors at 10 ng/mL: βNGF (Gibco 450–34), BDNF (Gibco 450– 02), colony stimulating factor- 2 (CSF2; Gibco 315–03), CSF3 (Gibco AF- 250- 05), fractalkine (CX3CL1; Gibco 300–31), IFNɣ (ThermoFisher Scientific BMS326), IGF1 (Gibco 250–19), IL17A (Gibco 210–17), IL1ɑ (R&D Systems 400- ML- 005), IL1β (R&D Systems 401- ML- 005), interleukin- 3 (IL3; Gibco 213–13), IL33 (Gibco 210–33), IL4 (Gibco 214–14), IL5 (Gibco 215–15), LPA (Sigma Aldrich 857128P), NTS (R&D Systems 1909), NRG1 (Gibco 100–03), pleiotrophin (PTN; R&D Systems 6580- PL), SCF (Gibco 250–03), sphingosine- 1- phosphate (R&D Systems 1370), tumor necrosis factor ɑ (TNFɑ; Gibco 315- 01A), VEGF (R&D Systems 493- MV- 005), and WNT3A (Gibco 315–20). .. For all CM screens, we applied CM at a 1:1 ratio with MGM to ensure microglial health for 72 h. For further investigation of IL1ɑ and IL1β ACM, we spiked microglia media lacking BSA, transferrin, and insulin with each CM at 200 μg/mL total protein and treated for 72 h. For all microglial proliferation assessments, we fixed microglia by diluting 8% paraformaldehyde (Sigma- Aldrich 441244) prepared in 0.1 M phosphate buffer (sodium phosphate dibasic anhydrous; Fisher Scientific, Cat. No. 374–1 and sodium dihydrogen phosphate monohydrate; Anachemia, Cat. No. 10049215 in water) 1:1 with media within the well for a final concentration of 4% paraformaldehyde for 10 min. We washed microglia with DPBS+/+ then blocked with 10% normal donkey serum (Sigma- Aldrich D9663) in 0.1% Triton- X- 100 (FisherScientific BP151- 500) in DPBS+/+ for 30–45 min at room temperature.

    Article Title: Inflammatory Mediators Both Directly and Indirectly Promote Microglial Proliferation
    Article Snippet: .. After 5 days in vitro, we treated microglia with the following factors at 10 ng/mL: βNGF (Gibco 450–34), BDNF (Gibco 450–02), colony stimulating factor‐2 (CSF2; Gibco 315–03), CSF3 (Gibco AF‐250‐05), fractalkine (CX3CL1; Gibco 300–31), IFNɣ (ThermoFisher Scientific BMS326), IGF1 (Gibco 250–19), IL17A (Gibco 210–17), IL1ɑ (R&D Systems 400‐ML‐005), IL1β (R&D Systems 401‐ML‐005), interleukin‐3 (IL3; Gibco 213–13), IL33 (Gibco 210–33), IL4 (Gibco 214–14), IL5 (Gibco 215–15), LPA (Sigma Aldrich 857128P), NTS (R&D Systems 1909), NRG1 (Gibco 100–03), pleiotrophin (PTN; R&D Systems 6580‐PL), SCF (Gibco 250–03), sphingosine‐1‐phosphate (R&D Systems 1370), tumor necrosis factor ɑ (TNFɑ; Gibco 315‐01A), VEGF (R&D Systems 493‐MV‐005), and WNT3A (Gibco 315–20). .. For all CM screens, we applied CM at a 1:1 ratio with MGM to ensure microglial health for 72 h. For further investigation of IL1ɑ and IL1β ACM, we spiked microglia media lacking BSA, transferrin, and insulin with each CM at 200 μg/mL total protein and treated for 72 h. For all microglial proliferation assessments, we fixed microglia by diluting 8% paraformaldehyde (Sigma‐Aldrich 441244) prepared in 0.1 M phosphate buffer (sodium phosphate dibasic anhydrous; Fisher Scientific, Cat. No. 374–1 and sodium dihydrogen phosphate monohydrate; Anachemia, Cat. No. 10049215 in water) 1:1 with media within the well for a final concentration of 4% paraformaldehyde for 10 min. We washed microglia with DPBS +/+ then blocked with 10% normal donkey serum (Sigma‐Aldrich D9663) in 0.1% Triton‐X‐100 (FisherScientific BP151‐500) in DPBS +/+ for 30–45 min at room temperature.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Immunomodulatory role of megakaryocytes in the hematopoietic niche of myeloproliferative neoplasms.
    Article Snippet: .. Cytokines levels were measured using ELISA kits following the manufacturer’s instructions: PF4 (R&D Systems, Cat. MCX400), TGFβ (R&D Systems, Cat. DB100C), IL1β (R&D Systems, Cat. MLB00C), TNFα (R&D Systems, Cat. MTA00B-1), IL6 (R&D Systems, Cat. M6000B-1) ELISA kit according. ..

    other:

    Article Title: Metabolic and behavioural maladaptations have a rapid onset following short-term obesogenic diet withdrawal in male wistar rats
    Article Snippet: The concentrations of IL6 (#DY506, R&D Systems, US), IL10 (#DY522, R&D Systems, US), IL1β (#DY501, R&D Systems, US), and TNFα (#DY510, R&D Systems, US) were evaluated in the supernatant following the manufacturer’s instructions.

    Purification:

    Article Title: GPR43 in eosinophils suppresses the emergence of pathogenic Siglec-F hi neutrophils in allergic airway inflammation in mice.
    Article Snippet: .. For cytokine-induced differentiation of Siglec-Fhi neutrophils, 0.2 million neutrophils purified from thebonemarrowwere cultured in RPMIbased media supplemented with 10% FBS (Hyclone, Catalogue#SH30919.03), 1mM non-essential amino acids, 5mM HEPES, 100 U/ml Penicillin, 100 μg/ml Streptomycin, 1mM sodium pyruvate, 50 μg/ml gentamicin (Gibco, USA, Catalogue#15750-060), 55 μM βmercaptoethanol (Sigma-Aldrich, Catalogue#M6250), and 2 mM L-glutamine (Gibco, Catalogue#25030-081) for 2 days in the presence of 10 ng/ml of each cytokine, including IL-1α (Catalogue#400-ML), IL1β (Catalogue#401-ML), IL-1Ra (Catalogue#480-RM), IL-2 (Catalogue#402-ML), IL-4 (Catalogue#404-ML), IL-5 (Catalogue#405-ML), IL8 (Catalogue#208-IL-010), IL-10 (Catalogue#417-ML), IL-12 (Catalogue#419-ML), IL-17A (Catalogue#421-ML), IL-33 (Catalogue#3626-ML), IFNγ (Catalogue#485-ML) (all from R&D Systems), IL-6 (BD Biosciences, Catalogue#554582), TNF (BD Biosciences, Catalogue#554589), GM-CSF (Peprotech, Catalogue#315-03), M-CSF (SigmaAldrich, Catalogue#SRP3110), and TGFβ (Miltenyi Biotec, Catalogue#130-095-066), or 10 μg/ml LPS (Sigma-Aldrich, Catalogue#L4391). ..

    Cell Culture:

    Article Title: GPR43 in eosinophils suppresses the emergence of pathogenic Siglec-F hi neutrophils in allergic airway inflammation in mice.
    Article Snippet: .. For cytokine-induced differentiation of Siglec-Fhi neutrophils, 0.2 million neutrophils purified from thebonemarrowwere cultured in RPMIbased media supplemented with 10% FBS (Hyclone, Catalogue#SH30919.03), 1mM non-essential amino acids, 5mM HEPES, 100 U/ml Penicillin, 100 μg/ml Streptomycin, 1mM sodium pyruvate, 50 μg/ml gentamicin (Gibco, USA, Catalogue#15750-060), 55 μM βmercaptoethanol (Sigma-Aldrich, Catalogue#M6250), and 2 mM L-glutamine (Gibco, Catalogue#25030-081) for 2 days in the presence of 10 ng/ml of each cytokine, including IL-1α (Catalogue#400-ML), IL1β (Catalogue#401-ML), IL-1Ra (Catalogue#480-RM), IL-2 (Catalogue#402-ML), IL-4 (Catalogue#404-ML), IL-5 (Catalogue#405-ML), IL8 (Catalogue#208-IL-010), IL-10 (Catalogue#417-ML), IL-12 (Catalogue#419-ML), IL-17A (Catalogue#421-ML), IL-33 (Catalogue#3626-ML), IFNγ (Catalogue#485-ML) (all from R&D Systems), IL-6 (BD Biosciences, Catalogue#554582), TNF (BD Biosciences, Catalogue#554589), GM-CSF (Peprotech, Catalogue#315-03), M-CSF (SigmaAldrich, Catalogue#SRP3110), and TGFβ (Miltenyi Biotec, Catalogue#130-095-066), or 10 μg/ml LPS (Sigma-Aldrich, Catalogue#L4391). ..



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