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il 17 a  (R&D Systems)


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    R&D Systems il 17 a
    Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001
    Il 17 A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+17a+f/Mouse+IL-17A%2FF+Heterodimer+DuoSet+ELISA/pmc12977735-198-21-27
    Average 94 stars, based on 16 article reviews
    il 17 a - by Bioz Stars, 2026-09
    94/100 stars

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    1) Product Images from "Mitochondria-targeted nanozyme system for psoriasis treatment"

    Article Title: Mitochondria-targeted nanozyme system for psoriasis treatment

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-026-04068-z

    Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001
    Figure Legend Snippet: Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001

    Techniques Used: Staining, Colorimetric Assay, Activity Assay, Expressing, Marker, Enzyme-linked Immunosorbent Assay

    Related Articles

    Recombinant:

    Article Title: IFN-γ Induction by Neutrophil-derived IL-17A Homodimer Augments Pulmonary Antibacterial Defense
    Article Snippet: IL-17A or IL-17F activity was blocked by i.p. administration of 1 μg of anti-IL-17A (monoclonal – rat IgG2a, Cat # MAB421/CF; R&D Systems), anti-IL-17F (polyclonal, Cat # AF2057/CF; R&D Systems), control Ab for anti-IL-17A (rat IgG2a, cat # MAB006/CF, R&D Systems) or control Ab for anti-IL-17F (goat IgG, cat # AB-108-C, R&D Systems) in A/J mice (Jackson). .. Recombinant IL-17A (Cat # 421-ML/CF, R&D Systems), IL-17A/F (Cat # 5390-IL/CF, R&D Systems), IL-17F (Cat # 2057-IL/CF, R&D Systems), or IFN-γ (Cat # 485-MI/CF, R&D Systems) was administered intratrachealy (i.t.) at a concentration of 1 μg/mouse whereas the control mice were administered with PBS supplemented with BSA (R&D Sysystems, MN). ..

    Article Title: IL-17RA–Mediated Epithelial Cell Activity Prevents Severe Inflammatory Response to Helicobacter pylori Infection
    Article Snippet: .. After 24–48 h, when monolayers were 80–90% confluent by visualization, the cells were stimulated with recombinant murine IL-17a, IL-17f, or IL-17a/f (R&D Systems) at 100 ng/ml for 12 h. RNA isolation was performed using a Qiagen microRNA kit per the manufacturer’s instructions and real-time qPCR was performed. ..

    Article Title: Cytokines IL-17, TNF and IFN-γ Alter the Expression of Antimicrobial Peptides and Proteins Disparately: A Targeted Proteomics Analysis using SOMAscan Technology
    Article Snippet: .. Recombinant human cytokines TNF, IFN-γ and IL-17A/F were obtained from R&D Systems. .. Anti-human lipocalin-2, elafin, cathepsin S and cathepsin V antibodies were obtained from Abcam (Toronto, ON, Canada).

    Article Title: Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors
    Article Snippet: .. We used recombinant human proteins such as IL-1β (50 ng/mL; R&D Systems), IL-2 (100 U/ml; BD Biosciences), IL-6 (50 ng/mL; BD PharMingen), IL-8/CXCL8 (50 ng/mL; R&D Systems), IL-10 (50 ng/mL; R&D Systems), IL-12 (50 ng/mL; R&D Systems), IL-17A/F (50 ng/mL; R&D Systems), IL-21 (100 ng/mL; R&D Systems), IL-22 (50 ng/mL; eBioscience), IL-23 (50 ng/mL; R&D Systems), IL-27 (50 ng/mL; R&D Systems), IFN-γ (0.1–100 ng/mL; R&D Systems), TNF-α (100 ng/mL; R&D Systems), TNFR1 (50 ng/mL; R&D Systems), TGF-β1 (50 ng/mL; R&D Systems), TGF-β2 (50 ng/mL; R&D Systems), GM-CSF (50 ng/mL; R&D Systems), MIG/CXCL9 (50 ng/mL; BD PharMingen), MCP-1/CCL2 (50 ng/mL; R&D Systems), MIP-3α/CCL20 (50 ng/mL; R&D Systems), macrophage migration inhibitory factor (50 ng/mL: R&D Systems), thrombospondin-1 (50 ng/mL: R&D Systems), and lipopolysaccharide (1,000 ng/mL: Sigma-Aldrich). .. The expression of Th1 cytokines, such as IFN-γ, IL-2, and TNF-α, in CD4 + T cells exposed to allogeneic iPS-RPE cells was assessed by intracellular staining (BD Cytofix/Cytoperm kits, BD PharMingen) followed by flow cytometry.

    Article Title: IL-17RA-Mediated Epithelial Cell Activity Prevents Severe Inflammatory Response to Helicobacter pylori Infection.
    Article Snippet: .. After 24 48 h, when monolayers were 80 90% confluent by visualization, the cells were stimulated with recombinant murine IL-17a, IL-17f, or IL-17a/f (R&D Systems) at 100 ng/ml for 12 h. RNA isolation was performed using a Qiagen microRNA kit per the manufacturer s instructions and real-time qPCR was performed. ..

    Article Title: Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors
    Article Snippet: .. We used recombinant human proteins such as IL-1b (50 ng/mL; R&D Systems), IL-2 (100 U/ml; BD Biosciences), IL-6 (50 ng/mL; BD PharMingen), IL-8/CXCL8 (50 ng/mL; R&D Systems), IL-10 (50 ng/mL; R&D Systems), IL-12 (50 ng/mL; R&D Systems), IL-17A/F (50 ng/mL; R&D Systems), IL-21 (100 ng/mL; R&D Systems), IL-22 (50 ng/mL; eBioscience), IL-23 (50 ng/mL; R&D Systems), IL-27 (50 ng/mL; R&D Systems), IFN-g (0.1–100 ng/mL; R&D Systems), TNF-a (100 ng/mL; R&D Systems), TNFR1 (50 ng/mL; R&D Systems), TGF-b1 (50 ng/mL; R&D Systems), TGF-b2 (50 ng/mL; R&D Systems), GM-CSF (50 ng/mL; R&D Systems), MIG/CXCL9 (50 ng/mL; BD PharMingen), MCP-1/CCL2 (50 ng/mL; R&D Systems), MIP-3a/ CCL20 (50 ng/mL; R&D Systems), macrophage migration inhibitory factor (50 ng/mL: R&D Systems), thrombospondin-1 (50 ng/mL: R&D Systems), and lipopolysaccharide (1,000 ng/mL: Sigma-Aldrich). .. The expression of Th1 cytokines, such as IFN-g, IL-2, and TNF-a, in CD4+ T cells exposed to allogeneic iPS-RPE cells was assessed by intracellular staining (BD Cytofix/Cytoperm kits, BD PharMingen) followed by flow cytometry.

    Concentration Assay:

    Article Title: IFN-γ Induction by Neutrophil-derived IL-17A Homodimer Augments Pulmonary Antibacterial Defense
    Article Snippet: IL-17A or IL-17F activity was blocked by i.p. administration of 1 μg of anti-IL-17A (monoclonal – rat IgG2a, Cat # MAB421/CF; R&D Systems), anti-IL-17F (polyclonal, Cat # AF2057/CF; R&D Systems), control Ab for anti-IL-17A (rat IgG2a, cat # MAB006/CF, R&D Systems) or control Ab for anti-IL-17F (goat IgG, cat # AB-108-C, R&D Systems) in A/J mice (Jackson). .. Recombinant IL-17A (Cat # 421-ML/CF, R&D Systems), IL-17A/F (Cat # 5390-IL/CF, R&D Systems), IL-17F (Cat # 2057-IL/CF, R&D Systems), or IFN-γ (Cat # 485-MI/CF, R&D Systems) was administered intratrachealy (i.t.) at a concentration of 1 μg/mouse whereas the control mice were administered with PBS supplemented with BSA (R&D Sysystems, MN). ..

    Control:

    Article Title: IFN-γ Induction by Neutrophil-derived IL-17A Homodimer Augments Pulmonary Antibacterial Defense
    Article Snippet: IL-17A or IL-17F activity was blocked by i.p. administration of 1 μg of anti-IL-17A (monoclonal – rat IgG2a, Cat # MAB421/CF; R&D Systems), anti-IL-17F (polyclonal, Cat # AF2057/CF; R&D Systems), control Ab for anti-IL-17A (rat IgG2a, cat # MAB006/CF, R&D Systems) or control Ab for anti-IL-17F (goat IgG, cat # AB-108-C, R&D Systems) in A/J mice (Jackson). .. Recombinant IL-17A (Cat # 421-ML/CF, R&D Systems), IL-17A/F (Cat # 5390-IL/CF, R&D Systems), IL-17F (Cat # 2057-IL/CF, R&D Systems), or IFN-γ (Cat # 485-MI/CF, R&D Systems) was administered intratrachealy (i.t.) at a concentration of 1 μg/mouse whereas the control mice were administered with PBS supplemented with BSA (R&D Sysystems, MN). ..

    Isolation:

    Article Title: IL-17RA–Mediated Epithelial Cell Activity Prevents Severe Inflammatory Response to Helicobacter pylori Infection
    Article Snippet: .. After 24–48 h, when monolayers were 80–90% confluent by visualization, the cells were stimulated with recombinant murine IL-17a, IL-17f, or IL-17a/f (R&D Systems) at 100 ng/ml for 12 h. RNA isolation was performed using a Qiagen microRNA kit per the manufacturer’s instructions and real-time qPCR was performed. ..

    Article Title: IL-17RA-Mediated Epithelial Cell Activity Prevents Severe Inflammatory Response to Helicobacter pylori Infection.
    Article Snippet: .. After 24 48 h, when monolayers were 80 90% confluent by visualization, the cells were stimulated with recombinant murine IL-17a, IL-17f, or IL-17a/f (R&D Systems) at 100 ng/ml for 12 h. RNA isolation was performed using a Qiagen microRNA kit per the manufacturer s instructions and real-time qPCR was performed. ..

    Real-time Polymerase Chain Reaction:

    Article Title: IL-17RA–Mediated Epithelial Cell Activity Prevents Severe Inflammatory Response to Helicobacter pylori Infection
    Article Snippet: .. After 24–48 h, when monolayers were 80–90% confluent by visualization, the cells were stimulated with recombinant murine IL-17a, IL-17f, or IL-17a/f (R&D Systems) at 100 ng/ml for 12 h. RNA isolation was performed using a Qiagen microRNA kit per the manufacturer’s instructions and real-time qPCR was performed. ..

    Article Title: IL-17RA-Mediated Epithelial Cell Activity Prevents Severe Inflammatory Response to Helicobacter pylori Infection.
    Article Snippet: .. After 24 48 h, when monolayers were 80 90% confluent by visualization, the cells were stimulated with recombinant murine IL-17a, IL-17f, or IL-17a/f (R&D Systems) at 100 ng/ml for 12 h. RNA isolation was performed using a Qiagen microRNA kit per the manufacturer s instructions and real-time qPCR was performed. ..

    Transfection:

    Article Title: IL-17-Mediated Downregulation of miR-101 Facilitates the Expression of EZH2 to Promote Epidermal Hyperplasia in Psoriasis.
    Article Snippet: Transfection was carried out using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions. .. IL-17A/F (number 5837-IL, 25 ng/ml, R&D Systems, Minneapolis, MN) treatment was initiated 6 hours after miRNA transfection. ..

    Migration:

    Article Title: Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors
    Article Snippet: .. We used recombinant human proteins such as IL-1β (50 ng/mL; R&D Systems), IL-2 (100 U/ml; BD Biosciences), IL-6 (50 ng/mL; BD PharMingen), IL-8/CXCL8 (50 ng/mL; R&D Systems), IL-10 (50 ng/mL; R&D Systems), IL-12 (50 ng/mL; R&D Systems), IL-17A/F (50 ng/mL; R&D Systems), IL-21 (100 ng/mL; R&D Systems), IL-22 (50 ng/mL; eBioscience), IL-23 (50 ng/mL; R&D Systems), IL-27 (50 ng/mL; R&D Systems), IFN-γ (0.1–100 ng/mL; R&D Systems), TNF-α (100 ng/mL; R&D Systems), TNFR1 (50 ng/mL; R&D Systems), TGF-β1 (50 ng/mL; R&D Systems), TGF-β2 (50 ng/mL; R&D Systems), GM-CSF (50 ng/mL; R&D Systems), MIG/CXCL9 (50 ng/mL; BD PharMingen), MCP-1/CCL2 (50 ng/mL; R&D Systems), MIP-3α/CCL20 (50 ng/mL; R&D Systems), macrophage migration inhibitory factor (50 ng/mL: R&D Systems), thrombospondin-1 (50 ng/mL: R&D Systems), and lipopolysaccharide (1,000 ng/mL: Sigma-Aldrich). .. The expression of Th1 cytokines, such as IFN-γ, IL-2, and TNF-α, in CD4 + T cells exposed to allogeneic iPS-RPE cells was assessed by intracellular staining (BD Cytofix/Cytoperm kits, BD PharMingen) followed by flow cytometry.

    Article Title: Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors
    Article Snippet: .. We used recombinant human proteins such as IL-1b (50 ng/mL; R&D Systems), IL-2 (100 U/ml; BD Biosciences), IL-6 (50 ng/mL; BD PharMingen), IL-8/CXCL8 (50 ng/mL; R&D Systems), IL-10 (50 ng/mL; R&D Systems), IL-12 (50 ng/mL; R&D Systems), IL-17A/F (50 ng/mL; R&D Systems), IL-21 (100 ng/mL; R&D Systems), IL-22 (50 ng/mL; eBioscience), IL-23 (50 ng/mL; R&D Systems), IL-27 (50 ng/mL; R&D Systems), IFN-g (0.1–100 ng/mL; R&D Systems), TNF-a (100 ng/mL; R&D Systems), TNFR1 (50 ng/mL; R&D Systems), TGF-b1 (50 ng/mL; R&D Systems), TGF-b2 (50 ng/mL; R&D Systems), GM-CSF (50 ng/mL; R&D Systems), MIG/CXCL9 (50 ng/mL; BD PharMingen), MCP-1/CCL2 (50 ng/mL; R&D Systems), MIP-3a/ CCL20 (50 ng/mL; R&D Systems), macrophage migration inhibitory factor (50 ng/mL: R&D Systems), thrombospondin-1 (50 ng/mL: R&D Systems), and lipopolysaccharide (1,000 ng/mL: Sigma-Aldrich). .. The expression of Th1 cytokines, such as IFN-g, IL-2, and TNF-a, in CD4+ T cells exposed to allogeneic iPS-RPE cells was assessed by intracellular staining (BD Cytofix/Cytoperm kits, BD PharMingen) followed by flow cytometry.



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    93
    MedChemExpress mouse il 17a f protein
    <t>IL-17A/F</t> <t>represses</t> <t>ECs</t> Ido1 transactivation and NAD + biosynthesis, and facilitates ECs senescence in the ischemic hindlimb muscle of young mice. A) Schematic diagram for testing methods was indicated. (B) Bar plot of the most prominent categories by KEGG pathway (left) and GO analysis (right). (C) Western blot analysis and quantification of IDO1 in hindlimb muscle ECs 7 days after HLI. PBS group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (D) RT-qPCR quantification of Ido1 in hindlimb muscle ECs 7 days after HLI. PBS group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05. (E) Western blot analysis and quantification of IDO1 and P16 in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns: not significant. (F–I) RT-qPCR quantification of Ido1 (F), p16 (G) , p21 (H) and Lmnb1 (I) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (J–L) ROS (J), NAD + (K) and NO (L) levels quantification in in hindlimb muscle ECs 7 days after HLI. Data of Con group in J was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (M) Venn diagram of IDO1 transcriptional factors between mouse and human. (N) Western blot analysis and quantification of P-CREB and total-CREB in hindlimb muscle ECs 7 days after HLI. Young group was set as 1. Unpaired two-tail t -test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001. (O, P) Western blot analysis and quantification of P-CREB, total-CREB (T-CREB) (O), IDO1(P) and P16 (P) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (Q–T) RT-qPCR quantification of Ido1 (Q), p16 (R) , p21 (S) and Lmnb1 (T) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ns: not significant. (U–W) ROS (U), NAD + (V) and NO (W) levels quantification in in hindlimb muscle ECs 7 days after HLI. Data of Con group in U was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant.
    Mouse Il 17a F Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+17a+f/IL-17A%2FF+Heterodimer%2C+Mouse/pmc12158617-63-14-49
    Average 93 stars, based on 1 article reviews
    mouse il 17a f protein - by Bioz Stars, 2026-09
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    recombinant human cytokine il 17 a f  (R&D Systems)
    95
    R&D Systems recombinant human cytokine il 17 a f
    <t>IL-17A/F</t> <t>represses</t> <t>ECs</t> Ido1 transactivation and NAD + biosynthesis, and facilitates ECs senescence in the ischemic hindlimb muscle of young mice. A) Schematic diagram for testing methods was indicated. (B) Bar plot of the most prominent categories by KEGG pathway (left) and GO analysis (right). (C) Western blot analysis and quantification of IDO1 in hindlimb muscle ECs 7 days after HLI. PBS group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (D) RT-qPCR quantification of Ido1 in hindlimb muscle ECs 7 days after HLI. PBS group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05. (E) Western blot analysis and quantification of IDO1 and P16 in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns: not significant. (F–I) RT-qPCR quantification of Ido1 (F), p16 (G) , p21 (H) and Lmnb1 (I) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (J–L) ROS (J), NAD + (K) and NO (L) levels quantification in in hindlimb muscle ECs 7 days after HLI. Data of Con group in J was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (M) Venn diagram of IDO1 transcriptional factors between mouse and human. (N) Western blot analysis and quantification of P-CREB and total-CREB in hindlimb muscle ECs 7 days after HLI. Young group was set as 1. Unpaired two-tail t -test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001. (O, P) Western blot analysis and quantification of P-CREB, total-CREB (T-CREB) (O), IDO1(P) and P16 (P) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (Q–T) RT-qPCR quantification of Ido1 (Q), p16 (R) , p21 (S) and Lmnb1 (T) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ns: not significant. (U–W) ROS (U), NAD + (V) and NO (W) levels quantification in in hindlimb muscle ECs 7 days after HLI. Data of Con group in U was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant.
    Recombinant Human Cytokine Il 17 A F, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+17a+f/Recombinant+Human+IL-17A+Protein%2C+CF/pm40410820-53-0-10
    Average 95 stars, based on 1 article reviews
    recombinant human cytokine il 17 a f - by Bioz Stars, 2026-09
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    Image Search Results


    Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001

    Journal: Journal of Nanobiotechnology

    Article Title: Mitochondria-targeted nanozyme system for psoriasis treatment

    doi: 10.1186/s12951-026-04068-z

    Figure Lengend Snippet: Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001

    Article Snippet: Cytokine levels in cell culture supernatants and tissue homogenates were measured using ELISA kits for IL-1β (DY401), IL-6 (M6000B), TNF-α (MTA00B), IL-17 A (DY5390), and IL-23 (M2300) (R&D Systems, USA).

    Techniques: Staining, Colorimetric Assay, Activity Assay, Expressing, Marker, Enzyme-linked Immunosorbent Assay

    (A) Inhibition of IL-17 bioactivity as secreted by human TH17 cells and assayed by a reporter cell assay. Dotted lines represent positive and negative assay controls. 1 representative of 7 experiments with different donors is shown. The error bars represent the SEM. (B, C) Evaluation of DC-806 in rat CIA. Rats were randomized on Day 11 and dosed as indicated. Daily measurements of ankle thickness (B) and terminal measurement of footpad weights (C) were used as efficacy readouts. (D) Preclinical evaluation of DC-806 serum levels. PK sampling for exposure determination was performed on Days 11, 16, at 1 and 12 hours post dose. The dotted lines represent the uncorrected IC 50 for rat IL-17AA and rat IL-17AF as listed in . The dashed line represents the LLQ of the quantification assay. All error bars represent the SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Abbreviations : anti-F, anti-IL-17F; BID, twice daily; CIA, collagen-induced arthritis; Dex, dexamethasone; IC 50 , half-maximal inhibitory concentration; IL-17, interleukin-17; LLQ, lower limit of quantification; PK, pharmacokinetic; QD, once daily; SEM, standard error of mean; TH17, T-helper 17.

    Journal: PLOS One

    Article Title: Clinical proof of concept for small molecule mediated inhibition of IL-17 in psoriasis

    doi: 10.1371/journal.pone.0341049

    Figure Lengend Snippet: (A) Inhibition of IL-17 bioactivity as secreted by human TH17 cells and assayed by a reporter cell assay. Dotted lines represent positive and negative assay controls. 1 representative of 7 experiments with different donors is shown. The error bars represent the SEM. (B, C) Evaluation of DC-806 in rat CIA. Rats were randomized on Day 11 and dosed as indicated. Daily measurements of ankle thickness (B) and terminal measurement of footpad weights (C) were used as efficacy readouts. (D) Preclinical evaluation of DC-806 serum levels. PK sampling for exposure determination was performed on Days 11, 16, at 1 and 12 hours post dose. The dotted lines represent the uncorrected IC 50 for rat IL-17AA and rat IL-17AF as listed in . The dashed line represents the LLQ of the quantification assay. All error bars represent the SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Abbreviations : anti-F, anti-IL-17F; BID, twice daily; CIA, collagen-induced arthritis; Dex, dexamethasone; IC 50 , half-maximal inhibitory concentration; IL-17, interleukin-17; LLQ, lower limit of quantification; PK, pharmacokinetic; QD, once daily; SEM, standard error of mean; TH17, T-helper 17.

    Article Snippet: To determine cellular potency, recombinant human (h)IL-17AA (Genscript cat# Z03228), hIL-17AF (R&D cat# 5837-IL-010), hIL-17FF (R&D Systems 1335-IL), rat IL-17AA (R&D systems 8410-IL-025), and rat IL-17AF (R&D systems 9340-IL-025) were used for in vitro stimulation at the concentrations listed in .

    Techniques: Inhibition, Sampling, Concentration Assay

    IL-17A/F represses ECs Ido1 transactivation and NAD + biosynthesis, and facilitates ECs senescence in the ischemic hindlimb muscle of young mice. A) Schematic diagram for testing methods was indicated. (B) Bar plot of the most prominent categories by KEGG pathway (left) and GO analysis (right). (C) Western blot analysis and quantification of IDO1 in hindlimb muscle ECs 7 days after HLI. PBS group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (D) RT-qPCR quantification of Ido1 in hindlimb muscle ECs 7 days after HLI. PBS group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05. (E) Western blot analysis and quantification of IDO1 and P16 in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns: not significant. (F–I) RT-qPCR quantification of Ido1 (F), p16 (G) , p21 (H) and Lmnb1 (I) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (J–L) ROS (J), NAD + (K) and NO (L) levels quantification in in hindlimb muscle ECs 7 days after HLI. Data of Con group in J was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (M) Venn diagram of IDO1 transcriptional factors between mouse and human. (N) Western blot analysis and quantification of P-CREB and total-CREB in hindlimb muscle ECs 7 days after HLI. Young group was set as 1. Unpaired two-tail t -test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001. (O, P) Western blot analysis and quantification of P-CREB, total-CREB (T-CREB) (O), IDO1(P) and P16 (P) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (Q–T) RT-qPCR quantification of Ido1 (Q), p16 (R) , p21 (S) and Lmnb1 (T) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ns: not significant. (U–W) ROS (U), NAD + (V) and NO (W) levels quantification in in hindlimb muscle ECs 7 days after HLI. Data of Con group in U was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant.

    Journal: Redox Biology

    Article Title: IDO1 improves postischemic neovascularization in aged mice by boosting endothelial NAD + de novo synthesis and curbing endothelial senescence

    doi: 10.1016/j.redox.2025.103695

    Figure Lengend Snippet: IL-17A/F represses ECs Ido1 transactivation and NAD + biosynthesis, and facilitates ECs senescence in the ischemic hindlimb muscle of young mice. A) Schematic diagram for testing methods was indicated. (B) Bar plot of the most prominent categories by KEGG pathway (left) and GO analysis (right). (C) Western blot analysis and quantification of IDO1 in hindlimb muscle ECs 7 days after HLI. PBS group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (D) RT-qPCR quantification of Ido1 in hindlimb muscle ECs 7 days after HLI. PBS group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05. (E) Western blot analysis and quantification of IDO1 and P16 in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001, ns: not significant. (F–I) RT-qPCR quantification of Ido1 (F), p16 (G) , p21 (H) and Lmnb1 (I) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (J–L) ROS (J), NAD + (K) and NO (L) levels quantification in in hindlimb muscle ECs 7 days after HLI. Data of Con group in J was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (M) Venn diagram of IDO1 transcriptional factors between mouse and human. (N) Western blot analysis and quantification of P-CREB and total-CREB in hindlimb muscle ECs 7 days after HLI. Young group was set as 1. Unpaired two-tail t -test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001. (O, P) Western blot analysis and quantification of P-CREB, total-CREB (T-CREB) (O), IDO1(P) and P16 (P) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (Q–T) RT-qPCR quantification of Ido1 (Q), p16 (R) , p21 (S) and Lmnb1 (T) in hindlimb muscle ECs 7 days after HLI. Con group was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ns: not significant. (U–W) ROS (U), NAD + (V) and NO (W) levels quantification in in hindlimb muscle ECs 7 days after HLI. Data of Con group in U was set as 1. One-way ANOVA with Tukey multiple comparison test, n = 3 per group, data are mean ± SEM, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant.

    Article Snippet: Mouse IL-17A protein (Catalog #HY- P70753 ), mouse IL-17F protein (Catalog #HY- P72584 ), mouse IL-17A/F protein (Catalog #HY- P77707 ), mouse IDO1 protein (Catalog #HY- P72616 ), IgG (Catalog #HY-P99002), brodalumab (Catalog #HY-P9925, an anti-interleukin-17-receptor IgG2 monoclonal antibody), and 666–15 (Catalog #HY-101120, CREB phosphorylation inhibitor) were purchased from Med-ChemExpress.

    Techniques: Western Blot, Comparison, Quantitative RT-PCR

    IL-17A/F counteracts Endothelial Ido1 overexpression-induced neovascularization under conditions of lower limb ischemia. (A) Scheme showing the experiment for rescuing the IL-17A/F-treated young mice by endothelial Ido1 overexpression. (B and C) Representative laser Doppler images (B) of hindlimbs of mice and quantification (C) of relative perfusion recovery as the blood flow ratio of the left limb to that of the right limb in AAV-NC + PBS, AAV-NC + IL-17A/F, AAV- Ido1 + PBS and AAV- Ido1 + IL-17A/F mice before and after HLI at the indicated time points. The areas under the curves of relative perfusion are shown, Two-way ANOVA with Tukey multiple comparison test, n = 6 per group, data are mean ± SEM, ∗AAV-NC + IL-17A/F vs. AAV-NC + PBS; # AAV- Ido1 + IL-17A/F vs. AAV-NC+ IL-17A/F, #/ ∗ P < 0.05, ### P < 0.001, ∗∗∗∗/ #### P < 0.0001. (D) Representative micro-CT images of ischemic hindlimbs 21 days after HLI. Scale bar, 3 mm. (E) Quantification of micro-CT analysis of arterial vasculature in ischemic hindlimbs. Two-way ANOVA with Tukey multiple comparison test, n = 6 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (F) Representative whole-mounted images of ischemic gracilis 21 days after HLI. (G) Quantification of the diameter of pre-existed collateral arteries (6 points per artery). One-way ANOVA with Tukey multiple comparison test, n = 6 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. (H) Immunofluorescence staining for CD31 in ischemic gastrocnemius muscles 21 days after HLI is shown. Scale bar, 200 μm. (I) Quantification of the CD31-positive area in muscle. One-way ANOVA with Tukey multiple comparison test, n = 6 per group, data are mean ± SEM, ∗∗∗∗ P < 0.0001.

    Journal: Redox Biology

    Article Title: IDO1 improves postischemic neovascularization in aged mice by boosting endothelial NAD + de novo synthesis and curbing endothelial senescence

    doi: 10.1016/j.redox.2025.103695

    Figure Lengend Snippet: IL-17A/F counteracts Endothelial Ido1 overexpression-induced neovascularization under conditions of lower limb ischemia. (A) Scheme showing the experiment for rescuing the IL-17A/F-treated young mice by endothelial Ido1 overexpression. (B and C) Representative laser Doppler images (B) of hindlimbs of mice and quantification (C) of relative perfusion recovery as the blood flow ratio of the left limb to that of the right limb in AAV-NC + PBS, AAV-NC + IL-17A/F, AAV- Ido1 + PBS and AAV- Ido1 + IL-17A/F mice before and after HLI at the indicated time points. The areas under the curves of relative perfusion are shown, Two-way ANOVA with Tukey multiple comparison test, n = 6 per group, data are mean ± SEM, ∗AAV-NC + IL-17A/F vs. AAV-NC + PBS; # AAV- Ido1 + IL-17A/F vs. AAV-NC+ IL-17A/F, #/ ∗ P < 0.05, ### P < 0.001, ∗∗∗∗/ #### P < 0.0001. (D) Representative micro-CT images of ischemic hindlimbs 21 days after HLI. Scale bar, 3 mm. (E) Quantification of micro-CT analysis of arterial vasculature in ischemic hindlimbs. Two-way ANOVA with Tukey multiple comparison test, n = 6 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, ns: not significant. (F) Representative whole-mounted images of ischemic gracilis 21 days after HLI. (G) Quantification of the diameter of pre-existed collateral arteries (6 points per artery). One-way ANOVA with Tukey multiple comparison test, n = 6 per group, data are mean ± SEM, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. (H) Immunofluorescence staining for CD31 in ischemic gastrocnemius muscles 21 days after HLI is shown. Scale bar, 200 μm. (I) Quantification of the CD31-positive area in muscle. One-way ANOVA with Tukey multiple comparison test, n = 6 per group, data are mean ± SEM, ∗∗∗∗ P < 0.0001.

    Article Snippet: Mouse IL-17A protein (Catalog #HY- P70753 ), mouse IL-17F protein (Catalog #HY- P72584 ), mouse IL-17A/F protein (Catalog #HY- P77707 ), mouse IDO1 protein (Catalog #HY- P72616 ), IgG (Catalog #HY-P99002), brodalumab (Catalog #HY-P9925, an anti-interleukin-17-receptor IgG2 monoclonal antibody), and 666–15 (Catalog #HY-101120, CREB phosphorylation inhibitor) were purchased from Med-ChemExpress.

    Techniques: Over Expression, Comparison, Micro-CT, Immunofluorescence, Staining, Muscles