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igg2b isotype control antibody  (ATCC)
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The Role of CD4 + T Cells in Cardiac Functional Remodeling (A) Experimental design. (B and C) Flow cytometric analysis of CD4 + T cells in peripheral blood of TCRM mice treated with <t>IgG2b</t> isotype control or anti-CD4 antibody. (D) Representative H&E and PSR-stained heart sections. (E) Myocarditis score and (F) quantification of PSR-positive area, in 8-week-old TCRM mice subjected to the indicated treatment. (G to J) Echocardiographic assessment of left ventricular morphology and function in 8-week-old TCRM mice treated with isotype control or anti-CD4 antibody. (G) LVID systole, (H) LVEF, (I) GCS, (J) GLS. (C, E to J) Pooled data from 3 independent experiments, including n = 12 anti-CD4-treated TCRM mice and n = 13 isotype-treated TCRM mice of which n = 5 progressed to iCMP. Statistical analysis was performed using C, unpaired 2-tailed Student’s t -test or Mann-Whitney U test and (E to J) 1-way analysis of variance with Tukey’s post hoc test or Kruskal-Wallis test with Dunnett’s multiple comparisons test. Boxplots as in . ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Abbreviations as in and .
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The Role of CD4 + T Cells in Cardiac Functional Remodeling (A) Experimental design. (B and C) Flow cytometric analysis of CD4 + T cells in peripheral blood of TCRM mice treated with <t>IgG2b</t> isotype control or anti-CD4 antibody. (D) Representative H&E and PSR-stained heart sections. (E) Myocarditis score and (F) quantification of PSR-positive area, in 8-week-old TCRM mice subjected to the indicated treatment. (G to J) Echocardiographic assessment of left ventricular morphology and function in 8-week-old TCRM mice treated with isotype control or anti-CD4 antibody. (G) LVID systole, (H) LVEF, (I) GCS, (J) GLS. (C, E to J) Pooled data from 3 independent experiments, including n = 12 anti-CD4-treated TCRM mice and n = 13 isotype-treated TCRM mice of which n = 5 progressed to iCMP. Statistical analysis was performed using C, unpaired 2-tailed Student’s t -test or Mann-Whitney U test and (E to J) 1-way analysis of variance with Tukey’s post hoc test or Kruskal-Wallis test with Dunnett’s multiple comparisons test. Boxplots as in . ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Abbreviations as in and .
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The Role of CD4 + T Cells in Cardiac Functional Remodeling (A) Experimental design. (B and C) Flow cytometric analysis of CD4 + T cells in peripheral blood of TCRM mice treated with <t>IgG2b</t> isotype control or anti-CD4 antibody. (D) Representative H&E and PSR-stained heart sections. (E) Myocarditis score and (F) quantification of PSR-positive area, in 8-week-old TCRM mice subjected to the indicated treatment. (G to J) Echocardiographic assessment of left ventricular morphology and function in 8-week-old TCRM mice treated with isotype control or anti-CD4 antibody. (G) LVID systole, (H) LVEF, (I) GCS, (J) GLS. (C, E to J) Pooled data from 3 independent experiments, including n = 12 anti-CD4-treated TCRM mice and n = 13 isotype-treated TCRM mice of which n = 5 progressed to iCMP. Statistical analysis was performed using C, unpaired 2-tailed Student’s t -test or Mann-Whitney U test and (E to J) 1-way analysis of variance with Tukey’s post hoc test or Kruskal-Wallis test with Dunnett’s multiple comparisons test. Boxplots as in . ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Abbreviations as in and .
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The Role of CD4 + T Cells in Cardiac Functional Remodeling (A) Experimental design. (B and C) Flow cytometric analysis of CD4 + T cells in peripheral blood of TCRM mice treated with <t>IgG2b</t> isotype control or anti-CD4 antibody. (D) Representative H&E and PSR-stained heart sections. (E) Myocarditis score and (F) quantification of PSR-positive area, in 8-week-old TCRM mice subjected to the indicated treatment. (G to J) Echocardiographic assessment of left ventricular morphology and function in 8-week-old TCRM mice treated with isotype control or anti-CD4 antibody. (G) LVID systole, (H) LVEF, (I) GCS, (J) GLS. (C, E to J) Pooled data from 3 independent experiments, including n = 12 anti-CD4-treated TCRM mice and n = 13 isotype-treated TCRM mice of which n = 5 progressed to iCMP. Statistical analysis was performed using C, unpaired 2-tailed Student’s t -test or Mann-Whitney U test and (E to J) 1-way analysis of variance with Tukey’s post hoc test or Kruskal-Wallis test with Dunnett’s multiple comparisons test. Boxplots as in . ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Abbreviations as in and .
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The Role of CD4 + T Cells in Cardiac Functional Remodeling (A) Experimental design. (B and C) Flow cytometric analysis of CD4 + T cells in peripheral blood of TCRM mice treated with <t>IgG2b</t> isotype control or anti-CD4 antibody. (D) Representative H&E and PSR-stained heart sections. (E) Myocarditis score and (F) quantification of PSR-positive area, in 8-week-old TCRM mice subjected to the indicated treatment. (G to J) Echocardiographic assessment of left ventricular morphology and function in 8-week-old TCRM mice treated with isotype control or anti-CD4 antibody. (G) LVID systole, (H) LVEF, (I) GCS, (J) GLS. (C, E to J) Pooled data from 3 independent experiments, including n = 12 anti-CD4-treated TCRM mice and n = 13 isotype-treated TCRM mice of which n = 5 progressed to iCMP. Statistical analysis was performed using C, unpaired 2-tailed Student’s t -test or Mann-Whitney U test and (E to J) 1-way analysis of variance with Tukey’s post hoc test or Kruskal-Wallis test with Dunnett’s multiple comparisons test. Boxplots as in . ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Abbreviations as in and .
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rat lgg2b kappa isotype control  (MedChemExpress)
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The Role of CD4 + T Cells in Cardiac Functional Remodeling (A) Experimental design. (B and C) Flow cytometric analysis of CD4 + T cells in peripheral blood of TCRM mice treated with <t>IgG2b</t> isotype control or anti-CD4 antibody. (D) Representative H&E and PSR-stained heart sections. (E) Myocarditis score and (F) quantification of PSR-positive area, in 8-week-old TCRM mice subjected to the indicated treatment. (G to J) Echocardiographic assessment of left ventricular morphology and function in 8-week-old TCRM mice treated with isotype control or anti-CD4 antibody. (G) LVID systole, (H) LVEF, (I) GCS, (J) GLS. (C, E to J) Pooled data from 3 independent experiments, including n = 12 anti-CD4-treated TCRM mice and n = 13 isotype-treated TCRM mice of which n = 5 progressed to iCMP. Statistical analysis was performed using C, unpaired 2-tailed Student’s t -test or Mann-Whitney U test and (E to J) 1-way analysis of variance with Tukey’s post hoc test or Kruskal-Wallis test with Dunnett’s multiple comparisons test. Boxplots as in . ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Abbreviations as in and .
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irdye 800cw goat antimouse igg secondary antibody  (LI-COR)
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The Role of CD4 + T Cells in Cardiac Functional Remodeling (A) Experimental design. (B and C) Flow cytometric analysis of CD4 + T cells in peripheral blood of TCRM mice treated with <t>IgG2b</t> isotype control or anti-CD4 antibody. (D) Representative H&E and PSR-stained heart sections. (E) Myocarditis score and (F) quantification of PSR-positive area, in 8-week-old TCRM mice subjected to the indicated treatment. (G to J) Echocardiographic assessment of left ventricular morphology and function in 8-week-old TCRM mice treated with isotype control or anti-CD4 antibody. (G) LVID systole, (H) LVEF, (I) GCS, (J) GLS. (C, E to J) Pooled data from 3 independent experiments, including n = 12 anti-CD4-treated TCRM mice and n = 13 isotype-treated TCRM mice of which n = 5 progressed to iCMP. Statistical analysis was performed using C, unpaired 2-tailed Student’s t -test or Mann-Whitney U test and (E to J) 1-way analysis of variance with Tukey’s post hoc test or Kruskal-Wallis test with Dunnett’s multiple comparisons test. Boxplots as in . ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Abbreviations as in and .
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The Role of CD4 + T Cells in Cardiac Functional Remodeling (A) Experimental design. (B and C) Flow cytometric analysis of CD4 + T cells in peripheral blood of TCRM mice treated with IgG2b isotype control or anti-CD4 antibody. (D) Representative H&E and PSR-stained heart sections. (E) Myocarditis score and (F) quantification of PSR-positive area, in 8-week-old TCRM mice subjected to the indicated treatment. (G to J) Echocardiographic assessment of left ventricular morphology and function in 8-week-old TCRM mice treated with isotype control or anti-CD4 antibody. (G) LVID systole, (H) LVEF, (I) GCS, (J) GLS. (C, E to J) Pooled data from 3 independent experiments, including n = 12 anti-CD4-treated TCRM mice and n = 13 isotype-treated TCRM mice of which n = 5 progressed to iCMP. Statistical analysis was performed using C, unpaired 2-tailed Student’s t -test or Mann-Whitney U test and (E to J) 1-way analysis of variance with Tukey’s post hoc test or Kruskal-Wallis test with Dunnett’s multiple comparisons test. Boxplots as in . ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Abbreviations as in and .

Journal: JACC: Basic to Translational Science

Article Title: Cardiopathogenic T Cells Govern Progression and Functional Remodeling in Inflammatory Cardiomyopathy and Chronic Myocarditis

doi: 10.1016/j.jacbts.2025.101458

Figure Lengend Snippet: The Role of CD4 + T Cells in Cardiac Functional Remodeling (A) Experimental design. (B and C) Flow cytometric analysis of CD4 + T cells in peripheral blood of TCRM mice treated with IgG2b isotype control or anti-CD4 antibody. (D) Representative H&E and PSR-stained heart sections. (E) Myocarditis score and (F) quantification of PSR-positive area, in 8-week-old TCRM mice subjected to the indicated treatment. (G to J) Echocardiographic assessment of left ventricular morphology and function in 8-week-old TCRM mice treated with isotype control or anti-CD4 antibody. (G) LVID systole, (H) LVEF, (I) GCS, (J) GLS. (C, E to J) Pooled data from 3 independent experiments, including n = 12 anti-CD4-treated TCRM mice and n = 13 isotype-treated TCRM mice of which n = 5 progressed to iCMP. Statistical analysis was performed using C, unpaired 2-tailed Student’s t -test or Mann-Whitney U test and (E to J) 1-way analysis of variance with Tukey’s post hoc test or Kruskal-Wallis test with Dunnett’s multiple comparisons test. Boxplots as in . ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Abbreviations as in and .

Article Snippet: Mice were treated with 500 μg of anti-CD4 antibody (clone: YTS191, ECACC, 87072282) or IgG2b isotype control antibody (clone: MPC-11, ECACC, ATCC CCL-167) by intraperitoneal injection twice a week as demonstrated in the respective experiments.

Techniques: Functional Assay, Control, Staining, MANN-WHITNEY