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recombinant mouse ifn g  (R&D Systems)


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    Structured Review

    R&D Systems recombinant mouse ifn g
    Recombinant Mouse Ifn G, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ifn+g/Recombinant+Mouse+IFN-epsilon+Protein/pm41869322-110-11-17
    Average 94 stars, based on 29 article reviews
    recombinant mouse ifn g - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Progestogen-driven B7-H4 contributes to onco-fetal immune tolerance.
    Article Snippet: .. CXCL9 (DY492), CXCL10 (DY466), IFN-g (DY485), and TNF-a (DY410) ELISA sets from R&D Systems were used according to the manufacturer’s instructions. .. CXCL9 (DY492), CXCL10 (DY466), IFN-g (DY485), and TNF-a (DY410) ELISA sets from R&D Systems were used according to the manufacturer’s instructions.

    Cell Culture:

    Article Title: Lipocalin-2 promotes neutrophilic inflammation in nasal polyps and its value as biomarker.
    Article Snippet: Samples were rinsed in PBS with three antibiotics including penicillin (100 U/ml), streptomycin (100 mg/ml), and amphotericin B (2.5 mg/ml), digested overnight with 0.1% Pronase E (P5147, Sigma) at 4 C, and incubated in BEGM (CC3170, LONZA). .. The cells and nasal explants were stimulated with one of the following: dsRNA (25ug/ml, poly I: C; Sigma), TNF-a (100 ng/ml, 10291-TA-020, R&D Systems), IFN-g (25 ng/ml, 285-IF-100, R&D Systems), IL-17(100 ng/ ml, 317-ILB-050, R&D Systems), IL-4 (100 ng/ml, 204-IL-010, R&D Systems), IL-13 (100 ng/ml, 213-ILB-010, R&D Systems), LPS (1ug/ ml, tlrl-eblps, Invitrogen), Pam3CSK4 (1ug/ml, tlrl-pms, Invitrogen), Fsl-1 (100 ng/ml, tlrl-fsl, Invitrogen), and ODN2395 (1umol/l, tlrl2395, Invitrogen) for 12 h or 48 h. The cells were stimulated with LCN2 (1 ng/ml, 10 ng/ml, and 100 ng/ml) for 6 h or 24 h. Cultured cells and their supernatants were collected for further analysis. ..

    Article Title: Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses.
    Article Snippet: .. Human and mouse podocyte cell lines (HPC and MPC) were gifted by M. Saleem (Children’s Renal Unit, Bristol Royal Hospital for Children, University of Bristol, Bristol, United Kingdom) and Stuart J. Shankland (University of Washington, Seattle, Washington, USA), and were cultured as previously described.63,64 To detect the ability of podocytes to present antigens, MPCs were incubated with DQ-OVA or OVA (10 mg/mL, Invitrogen, Shanghai, PR China, O23021) in the presence or absence of IFN-a (30 ng/mL, 18 Cell Reports 43, 114249, May 28, 2024 Novoprotein, CK83), IFN-g (30 ng/mL, R&D Systems, 285-IF-100) or IL-17 (50 ng/mL, R&D Systems, C17-ILB-050). ..

    Incubation:

    Article Title: Cannabinoid CB 2 receptor controls chronic itch by regulating spinal microglial activation and synaptic transmission.
    Article Snippet: The mousemicroglial cell line, BV2 cells were cultured in MEMmedium (Gibco, 11095080) containing 10% FBS and 1% pen-strep in an atmosphere with 5% CO2 at 37 C. To detect protein, BV2 cells were seeded in a 6-well plate at the density of 250000 cells/well. .. After 1–2 days of seeding, cells were incubated for 6 h with IFN-g (300 U/mL, R&D Systems, 485-MI-100/CF) alone or after 1 h of pretreatment with CB2R selective agonist AM1241 (1 mM) or p38 inhibitor SB203580 (10 mM). ..

    Article Title: Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses.
    Article Snippet: .. Human and mouse podocyte cell lines (HPC and MPC) were gifted by M. Saleem (Children’s Renal Unit, Bristol Royal Hospital for Children, University of Bristol, Bristol, United Kingdom) and Stuart J. Shankland (University of Washington, Seattle, Washington, USA), and were cultured as previously described.63,64 To detect the ability of podocytes to present antigens, MPCs were incubated with DQ-OVA or OVA (10 mg/mL, Invitrogen, Shanghai, PR China, O23021) in the presence or absence of IFN-a (30 ng/mL, 18 Cell Reports 43, 114249, May 28, 2024 Novoprotein, CK83), IFN-g (30 ng/mL, R&D Systems, 285-IF-100) or IL-17 (50 ng/mL, R&D Systems, C17-ILB-050). ..



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    In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

    Journal: Bioactive Materials

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    doi: 10.1016/j.bioactmat.2026.02.018

    Figure Lengend Snippet: In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

    Article Snippet: Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay.

    Techniques: In Vivo, Activation Assay, Injection, Flow Cytometry, Enzyme-linked Immunospot