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b4 ib4 fl 1201 fluorescein  (Vector Laboratories)


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    Structured Review

    Vector Laboratories b4 ib4 fl 1201 fluorescein
    (a) Fluorescence staining images showing the distribution of liposomes in the choroidal flat-mounts 7 days after intravitreal injection, with neovascular lesions <t>(IB4,</t> green) and liposomes (Cy3, red). (b) Statistical results of fluorescence intensity for each group.
    B4 Ib4 Fl 1201 Fluorescein, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A cell membrane-hybridized nanocarrier-based RNA delivery system for ocular neovascular diseases"

    Article Title: A cell membrane-hybridized nanocarrier-based RNA delivery system for ocular neovascular diseases

    Journal: bioRxiv

    doi: 10.64898/2025.12.23.696136

    (a) Fluorescence staining images showing the distribution of liposomes in the choroidal flat-mounts 7 days after intravitreal injection, with neovascular lesions (IB4, green) and liposomes (Cy3, red). (b) Statistical results of fluorescence intensity for each group.
    Figure Legend Snippet: (a) Fluorescence staining images showing the distribution of liposomes in the choroidal flat-mounts 7 days after intravitreal injection, with neovascular lesions (IB4, green) and liposomes (Cy3, red). (b) Statistical results of fluorescence intensity for each group.

    Techniques Used: Fluorescence, Staining, Liposomes, Injection



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    b4 ib4 fl 1201 fluorescein  (Vector Laboratories)
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    Vector Laboratories b4 ib4 fl 1201 fluorescein
    (a) Fluorescence staining images showing the distribution of liposomes in the choroidal flat-mounts 7 days after intravitreal injection, with neovascular lesions <t>(IB4,</t> green) and liposomes (Cy3, red). (b) Statistical results of fluorescence intensity for each group.
    B4 Ib4 Fl 1201 Fluorescein, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ib4  (Vector Laboratories)
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    Vector Laboratories ib4
    Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and <t>IB4</t> (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.
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    fluorescein isothiocyanate fitc conjugated gsl ib4  (Vector Laboratories)
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    Vector Laboratories fluorescein isothiocyanate fitc conjugated gsl ib4
    Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. <t>FITC-conjugated</t> <t>GSL-IB4</t> was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.
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    labeled griffonia simplicifolia lectin ib4  (Vector Laboratories)
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    Vector Laboratories labeled griffonia simplicifolia lectin ib4
    Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. <t>FITC-conjugated</t> <t>GSL-IB4</t> was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.
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    hy p72782 ib4 vector laboratories  (Vector Laboratories)
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    Vector Laboratories hy p72782 ib4 vector laboratories
    Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. <t>FITC-conjugated</t> <t>GSL-IB4</t> was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.
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    fluorescein isothiocyanate 140 fitc conjugated isolectin gs ib4  (Vector Laboratories)
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    Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. <t>FITC-conjugated</t> <t>GSL-IB4</t> was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.
    Fluorescein Isothiocyanate 140 Fitc Conjugated Isolectin Gs Ib4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ib4 biotinylated mouse rat rabbit goat griffonia simplicifolia vector  (Vector Laboratories)
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    Vector Laboratories ib4 biotinylated mouse rat rabbit goat griffonia simplicifolia vector
    Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. <t>FITC-conjugated</t> <t>GSL-IB4</t> was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.
    Ib4 Biotinylated Mouse Rat Rabbit Goat Griffonia Simplicifolia Vector, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    isolectings ib4  (Vector Laboratories)
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    Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. <t>FITC-conjugated</t> <t>GSL-IB4</t> was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.
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    anti ib4 fitc  (Vector Laboratories)
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    Vector Laboratories anti ib4 fitc
    Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. <t>FITC-conjugated</t> <t>GSL-IB4</t> was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.
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    Image Search Results


    (a) Fluorescence staining images showing the distribution of liposomes in the choroidal flat-mounts 7 days after intravitreal injection, with neovascular lesions (IB4, green) and liposomes (Cy3, red). (b) Statistical results of fluorescence intensity for each group.

    Journal: bioRxiv

    Article Title: A cell membrane-hybridized nanocarrier-based RNA delivery system for ocular neovascular diseases

    doi: 10.64898/2025.12.23.696136

    Figure Lengend Snippet: (a) Fluorescence staining images showing the distribution of liposomes in the choroidal flat-mounts 7 days after intravitreal injection, with neovascular lesions (IB4, green) and liposomes (Cy3, red). (b) Statistical results of fluorescence intensity for each group.

    Article Snippet: Griffonia Simplicifolia Lectin I isolectin B4 (IB4) (FL-1201, Fluorescein) was purchased from vector laboratories (USA).

    Techniques: Fluorescence, Staining, Liposomes, Injection

    Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and IB4 (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.

    Journal: Frontiers in Physiology

    Article Title: Tmem45b modulates itch via endoplasmic reticulum calcium regulation

    doi: 10.3389/fphys.2025.1708686

    Figure Lengend Snippet: Tmem45b is primarily expressed in itch-sensing neurons. (A) Immunostaining results show the expression of Tmem45b (green), CGRP (red), and NF200 (red). (B) Immunostaining results show the co-expression of Tmem45b (green) and IB4 (red). (C) Statistical analysis shows the proportion of Tmem45b + IB4 + . (D) scRNA-seq (10x Genomics) analysis identified 16 subtypes of DRG neurons. Gene annotations for these 16 neuron types are shown in the right panel. Tmem45b is predominantly enriched in neurons positive for Mrgprd, Mrgpra3, Nppb , and Th . (E) RNAscope in situ hybridization shows co-localization among Tmem45b (green), Mrgprd (red) , Mrgpra3 (red), and Nppb (red). Immunostaining results show that Tmem45b (green) is co-expressed with Th (red). Arrows indicate co-expressing cells. Scale bar, 20 μm. (F) Proportions of Mrgprd + , Mrgpra3 + , Nppb + , and Th + neurons among Tmem45b + DRG neurons. DRG was obtained from at least 3 mice.

    Article Snippet: Antibodies used in this study: GM130 (BD, 610822), Th (Millipore, AB1542), IB4 (Vector, FL-1201-.5), Tuj1 (Starter, SDT-251-28), CGRP (Dia Sorin, 24112), NF200 (CST, 2836S), PDI (Santa Cruz, SC-20132), Calnexin (Abcam, ab112995), Serca1 (Proteintech, 22361-1-AP), mitochondria-tracer (Beyotime, C1048), TGN38 (Bio-Rad, AHP499G), GFAP (Millipore, MAB3402), IBA1 (Abcam, ab5076).

    Techniques: Immunostaining, Expressing, RNAscope, In Situ Hybridization

    Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.

    Journal: Regenerative Therapy

    Article Title: Assessment of permeability in deep tissue capillaries using a new method reflects the nutrient supply status in a healthy heart

    doi: 10.1016/j.reth.2025.10.005

    Figure Lengend Snippet: Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.

    Article Snippet: To visualize cardiac endothelial cells, fluorescein isothiocyanate (FITC) -conjugated GSL-IB4 (#FL-1201; Vector Laboratories Inc., Burlingame, CA, USA) was injected into the tail vein 5 min after TD 40 injection under anesthesia (1 mg/ml, 50 μl/10 g).

    Techniques: Labeling, Fluorescence, Injection, Microscopy, Software