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hydroxyapatite hap resin  (Bio-Rad)


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    Bio-Rad hydroxyapatite hap resin
    Hydroxyapatite Hap Resin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hydroxyapatite+resin+(hap/CHT+Ceramic+Hydroxyapatite/pm41102449-480-9-12
    Average 96 stars, based on 1343 article reviews
    hydroxyapatite hap resin - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: DOT1L O-GlcNAcylation promotes its protein stability and MLL-fusion leukemia cell proliferation.
    Article Snippet: Then 266 mL 50% HAP resin (Bio-Rad, 1582000) slurry in HAP Buffer 1 (5mM Phosphate buffer [pH 7.2 final], 600 mM NaCl and 1mM EDTA) was added and rotated in 4 C for 10 min. HAP resin was then washed once with 200 mL HAP buffer 1 and three times with 200 mL buffer 2 (5mM Phosphate buffer [pH 7.2], 100mM NaCl and 1mM EDTA).

    Article Title: Epigenetic alterations facilitate transcriptional and translational programs in hypoxia.
    Article Snippet: Soluble chromatin supernatant was added to 0.07 g of hydroxyapatite (HAP) resin (Bio-Rad 157-0021) rehydrated in HAP Buffer 1 (5 mM NaPO4, pH 7.2, 600 mM NaCl, 1 mM EDTA and 200 μM PMSF) and rotated for 10 min at 4 °C.

    Article Title: Epigenetic coordination of transcriptional and translational programs in hypoxia
    Article Snippet: 15 μL of soluble chromatin was saved for visualization and the rest of the soluble chromatin was added to 0.07 g of HAP resin (Bio-Rad, #157-0021) rehydrated in HAP Buffer 1 (5 mM NaPO4 pH 7.2, 600 mM NaCl, 1 mM EDTA, 200 uM PMSF) and rotated for 10 min at 4 °C.

    Article Title: Epigenetic alterations facilitate transcriptional and translational programs in hypoxia
    Article Snippet: Soluble chromatin supernatant was added to 0.07 g of hydroxyapatite (HAP) resin (Bio-Rad 157-0021) rehydrated in HAP Buffer 1 (5 mM NaPO 4 , pH 7.2, 600 mM NaCl, 1 mM EDTA and 200 μM PMSF) and rotated for 10 min at 4 °C.

    Article Title: Construction of an experimental study and addition of adapter sequences using HiDi DNA polymerase for improving DNA normalization methods relevant to novel gene discovery.
    Article Snippet: The entire mixed solution was added to a hydroxyapatite resin (HAP) (Bio-Rad Laboratories, Inc., California, USA) suspension that was pre-warmed at 60 ◦C (40 mg in 60 μL of 0.05 M sodium phosphate buffer (pH 6.8)).

    Article Title: Re-evaluating the role of nucleosomal bivalency in early development
    Article Snippet: Briefly, 66 mg of HAP resin (Bio-Rad Macro-Prep ® Ceramic Hydroxyapatite Type I 20 μm) was rehydrated with 200 μl of HAP buffer 1 (3.42 mM Na 2 HPO 4 and 1.58 mM NaH 2 PO 4 final pH 7.2, 600 mM NaCl, 1 mM EDTA, 200 μM PMSF).

    Incubation:

    Article Title: RNA mis-splicing drives viral mimicry response after DNMTi therapy in SETD2-mutant kidney cancer
    Article Snippet: Single nucleosomes were obtained by MNase digestion (1 unit Worthington MNase per 4.375 μg of chromatin at 37°C for 12 min) followed by hydroxyapatite chromatography purification. .. 100 μg fragmented chromatin were mixed with 66 mg of hydroxyapatite (HAP) resin (Bio-Rad Macro-Prep Ceramic Hydroxyapatite Type I 20 μm) rehydrated with 200 μL of HAP buffer 1(5mM sodium phosphate pH 7.2, 600 mM NaCl, 1 mM EDTA, 200 μM PMSF), incubated for 10 min at 4°C on rotator and was loaded to the centrifugal filter unit (Millipore Ultrafree MC–HV Centrifugal Filter 0.45 μm). ..

    Article Title: Compositions and methods for quantitative assessment of DNA-protein complex density
    Article Snippet: .. Fragmented chromatin with internal standard ladders were split into 100 μg total nucleic acid fractions and each fraction was mixed with 66 mg of hydroxyapatite (HAP) resin (Bio-Rad Macro-Prep® Ceramic Hydroxyapatite Type I 20 μm) rehydrated with 200 μL of HAP buffer 1 (3.42 mM Na2HPO4 and 1.58 mM NaH2PO4 final pH 7.2, 600 mM NaCl, 1 mM EDTA, 200 μM PMSF), incubated for 10 minutes at 4° C. on rotator and subsequently was applied to the centrifugal filter unit (Millipore Ultrafree® MC-HV Centrifugal Filter 0.45 μm). .. Fragmented chromatin with internal standard ladders were split into 100 μg total nucleic acid fractions and each fraction was mixed with 66 mg of hydroxyapatite (HAP) resin (Bio-Rad Macro-Prep Ceramic Hydroxyapatite Type I 20 μm) rehydrated with 200 μL of HAP buffer 1 (3.42 mM Na2HPO4 and 1.58 mM NaH2PO4 final pH 7.2, 600 mM NaCl, 1 mM EDTA, 200 μM PMSF), incubated for 10 minutes at 4° C. on rotator and subsequently was applied to the centrifugal filter unit (Millipore Ultrafree MC-HV Centrifugal Filter 0.45 μm).



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    (A) Chromatography scheme for purification of SCC-B from NT2 nuclear extracts (NT2 NE). NT2 NE is first subjected to ammonium sulfate precipitation (55% saturation) followed by a series of chromatographic columns including nickel affinity agarose (Ni-NTA), cation exchangers phosphocellulose (P11), heparin (Poros-HE), Mono S, anion exchanger <t>Poros-HQ,</t> <t>hydroxyapatite</t> <t>(HAP),</t> and gel filtration medium Superose 6. SCC-B activity segregates from SCC-A (Dyskerin (DKC1) RNP) at the Poros-HE step. (B) Input fraction containing SCC-B activity from the Poros-HE step (IN), flow-through (FT) and various salt-eluted Mono S fractions were assayed for their ability to stimulate OCT4/SOX2-dependent transcription from the human NANOG promoter template engineered with four extra copies of the oct-sox composite binding element (bottom). All reactions contain purified general transcription factors (GTFs), Pol II, OCT4, SOX2, and recombinant XPC and DKC1 complexes. (C) Fractions assayed in vitro transcription shown in (B) are separated on a 4-12% gradient polyacrylamide gel and stained with silver. Filled arrowhead indicates the predominant polypeptide at ~110 kDa that co-migrates with SCC-B transcriptional activity. The following figure supplement is available for : .
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    (A) Chromatography scheme for purification of SCC-B from NT2 nuclear extracts (NT2 NE). NT2 NE is first subjected to ammonium sulfate precipitation (55% saturation) followed by a series of chromatographic columns including nickel affinity agarose (Ni-NTA), cation exchangers phosphocellulose (P11), heparin (Poros-HE), Mono S, anion exchanger Poros-HQ, hydroxyapatite (HAP), and gel filtration medium Superose 6. SCC-B activity segregates from SCC-A (Dyskerin (DKC1) RNP) at the Poros-HE step. (B) Input fraction containing SCC-B activity from the Poros-HE step (IN), flow-through (FT) and various salt-eluted Mono S fractions were assayed for their ability to stimulate OCT4/SOX2-dependent transcription from the human NANOG promoter template engineered with four extra copies of the oct-sox composite binding element (bottom). All reactions contain purified general transcription factors (GTFs), Pol II, OCT4, SOX2, and recombinant XPC and DKC1 complexes. (C) Fractions assayed in vitro transcription shown in (B) are separated on a 4-12% gradient polyacrylamide gel and stained with silver. Filled arrowhead indicates the predominant polypeptide at ~110 kDa that co-migrates with SCC-B transcriptional activity. The following figure supplement is available for : .

    Journal: bioRxiv

    Article Title: ATP-binding cassette protein ABCF1 couples gene transcription with maintenance of genome integrity in embryonic stem cells

    doi: 10.1101/2020.05.28.122184

    Figure Lengend Snippet: (A) Chromatography scheme for purification of SCC-B from NT2 nuclear extracts (NT2 NE). NT2 NE is first subjected to ammonium sulfate precipitation (55% saturation) followed by a series of chromatographic columns including nickel affinity agarose (Ni-NTA), cation exchangers phosphocellulose (P11), heparin (Poros-HE), Mono S, anion exchanger Poros-HQ, hydroxyapatite (HAP), and gel filtration medium Superose 6. SCC-B activity segregates from SCC-A (Dyskerin (DKC1) RNP) at the Poros-HE step. (B) Input fraction containing SCC-B activity from the Poros-HE step (IN), flow-through (FT) and various salt-eluted Mono S fractions were assayed for their ability to stimulate OCT4/SOX2-dependent transcription from the human NANOG promoter template engineered with four extra copies of the oct-sox composite binding element (bottom). All reactions contain purified general transcription factors (GTFs), Pol II, OCT4, SOX2, and recombinant XPC and DKC1 complexes. (C) Fractions assayed in vitro transcription shown in (B) are separated on a 4-12% gradient polyacrylamide gel and stained with silver. Filled arrowhead indicates the predominant polypeptide at ~110 kDa that co-migrates with SCC-B transcriptional activity. The following figure supplement is available for : .

    Article Snippet: Transcriptionally active Q0.3 fraction (0.32–0.4 M) were pooled and applied directly to hydroxyapatite (HAP) type II ceramic resin (Bio-Rad), washed first at 0.38 M, then lowered to 0.1 M KCl in 3 CV.

    Techniques: Chromatography, Purification, Filtration, Activity Assay, Binding Assay, Recombinant, In Vitro, Staining