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huvec cell line  (PromoCell)


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    Structured Review

    PromoCell huvec cell line
    Huvec Cell Line, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 1575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/huvec+cell+line/HUVEC-c+Human+Umbilical+Vein+Endothelial+Cells/pm41744987-256-8-13
    Average 98 stars, based on 1575 article reviews
    huvec cell line - by Bioz Stars, 2026-09
    98/100 stars

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    In Vitro:

    Article Title: A New Cardiac Decellularized Extracellular Matrix (dECM)-Based Hydrogel: From Its Development with a Standardized Myocardial Decellularization Procedure to In Vitro Model Applications
    Article Snippet: .. The HUVEC cell line, provided by PromoCell ® (cryopreserved, PromoCell ® ), was employed to evaluate the cardiac dECM-based hydrogel’s feasibility of using it to develop in vitro cardiovascular models. .. The cell line was cultured on plastic tissue culture dishes (Corning) ( a) at a cell density of 5 × 10 3 cells/cm 2 in a cell incubator (Eppendorf CellXpert C170i) at 37 °C and 5% CO 2 in complete culture medium (EBM ® -2 Basal Medium supplemented with 2% FBS, 4 μL/mL hFGF-B, 1 μL/mL VEGF, 1 μL/mL R3-IGF-1, 1 μL/mL Ascorbic Acid, 1 μL/mL hEGF, 1 μL/mL GA-1000, 1 μL/mL Heparin, and 0.4 μL/mL Hydrocortisone (all from Lonza, Milan, Italy)).

    Article Title: The endothelial layer formation in the presence of AuNPs/CdSe/TaNPs-loaded PLCL/PVP-based electrospun nanofibers
    Article Snippet: .. In vitro tests were performed using the HUVEC cell line (Primary Human Umbilical Vein Endothelial Cells, PromoCell). .. Cells were cultured in complete Endothelial Cell Growth Medium (Cell Applications, Inc.) at 37oC in a humidified atmosphere and 5% CO2.

    Article Title: A New Cardiac Decellularized Extracellular Matrix (dECM)-Based Hydrogel: From Its Development with a Standardized Myocardial Decellularization Procedure to In Vitro Model Applications.
    Article Snippet: .. 4.5.1. dECM-Based Hydrogel Cardiovascular In Vitro Model The HUVEC cell line, provided by PromoCell® (cryopreserved, PromoCell®), was employed to evaluate the cardiac dECM-based hydrogel’s feasibility of using it to develop in vitro cardiovascular models. .. The cell line was cultured on plastic tissue culture dishes (Corning) (Figure 12a) at a cell density of 5 × 103 cells/cm2 in a cell incubator (Eppendorf CellXpert C170i) at 37 ◦C and 5% CO2 in complete culture medium (EBM®-2 Basal Medium supplemented with 2% FBS, 4 μL/mL hFGF-B, 1 μL/mL VEGF, 1 μL/mL R3-IGF1, 1 μL/mL Ascorbic Acid, 1 μL/mL hEGF, 1 μL/mL GA-1000, 1 μL/mL Heparin, and 0.4 μL/mL Hydrocortisone (all from Lonza, Milan, Italy)).

    Control:

    Article Title: An in vitro study on the effect of bevacizumab on endothelial cell proliferation and VEGF concentration level in patients with hereditary hemorrhagic telangiectasia
    Article Snippet: .. Endothelial cells from a HUVEC cell line (PromoCell; cat. no. C-12250) were used as the control. ..

    Cell Culture:

    Article Title: Vascularized Co-Culture Clusteroids of Primary Endothelial and Hep-G2 Cells Based on Aqueous Two-Phase Pickering Emulsions
    Article Snippet: Fetal bovine serum (FBS) was sourced from Labtech (Heathfield, UK) and trypsin-EDTA was purchased from Lonza (UK). .. Endothelial cell culture medium was purchased from ATCC, HUVEC cell line was sourced from Promocell (Lutterworth, Leicestershire, UK), and Hep-G2 cell line was purchased from ECACC cell collection (Salisbury, UK). .. The MMP-2 ELISA kit was purchased from GE healthcare (Amersham, UK), the IGFBP, VEGF, IL-8, and HIF1-α DuoSet ELISA kits and angiogenesis array kit (ARY007) were all purchased from Bio-Techne (Abingdon, UK).



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