hutu80 cells  (ATCC)

Journal: bioRxiv

Article Title: Human enterochromaffin cells and apical-out intestinal organoids as models for human sapovirus infection

doi: 10.64898/2026.03.06.710023

Figure Lengend Snippet: Phylogenetic tree of sapovirus samples (n = 27), color coded by genotype, and reference strains. The tree was constructed with Maximun likelihood algorithm using Kimura 2-parameter model (gamma distributed with invariant sites) and 1000 bootstrap replicates. Scale bar for branch lengths shows number of substitutions per site. Samples F20, F21, F30 and F31 were excluded in the analysis due to incomplete sequences. Reference strains: GII.1/Hu/IND/2021/NICED-BCH-12967 (GenBank: LC851686 ), GII.1/Hu/JPN/2013/Mie-493 (GenBank: LC816490 ), GII.1/Hu/BGD/2012/475050 (GenBank: MW088928 ), GII.1/Hu/UK/1998/Bristol (GenBank: AJ249939 ), GII.3/Hu/JPN/2023/20220111 (GenBank: LC848995 ), GII.3/Hu/JPN/2023/S22-415 (GenBank: LC849000 ), GII.3/Hu/JPN/2019/Mie-021 (GenBank: LC816544 ), GII.3/Hu/JPN/2017/Mie-835 (GenBank: LC816541 ), GI.1/Hu/US/2014/ Portland3639 (Genbank: MG012399 ), GI.1/Hu/IND/2022/NICED-BCH-13235 (GenBank: LC851695 ), GI.1/Hu/JPN/2011/EH-70 (GenBank: LC504316 ), GI.1/Hu/JPN/2023/V231401 (GenBank: LC848977 ), GI.1/Hu/JPN/1982/MT-2010 (GenBank: HM002617 ), GII.1/Hu/US/2015/Nashville9343 (GenBank: MG012444 ), GI.1/Hu/GER/pJG-Sap01/Dresden (GenBank: AY694184 ), GI.3/Hu/SWE/1997/318/Stockholm (GenBank: AF194182 ), GII.3/Hu/US/2015/Nashville9354 (GenBank: MG012419 ), GI.1/Hu/UK/1993/Manchester (GenBank: X86560 ). Abbreviations: Hu: Human, IND: India, JPN: Japan, US: United States, BGD: Bangladesh, GER: Germany, SWE: Sweden, UK: United Kingdom. B) Viral load (genome copies/gram of feces) and genotype of 31 sapovirus positive samples. Each colored square corresponds to one sample, and the median viral load is shown for each genotype. p-value, determined by Kruskal-Wallis test, is displayed. C) Detection of CD36 by gene expresison analysis and immunofluorescence staining in GOT1 and HuTu80. Gene expression of CD36 is displayed as inverted ΔCt value (1/ ΔCt), with ΔCt calculated by subtracting the Ct-value for CD36 to the Ct of the house-keeping gene GAPDH. The fold difference in expression between HuTu80 and GOT1 is shown above the bars. D - F) Replication of sapovirus samples in HuTu80 and GOT1. Left y-axis represents the fold change (FC) of virus at 72 hours post infection (hpi) compared to 2 hpi. The two biological duplicates per infection experiment are shown as black dots. A mean FC of at least 5-fold (dotted line) was considered successful replication and samples with a mean FC of less than 1 were set to 1.1 FC for visualization purposes in the graph. The sample viral load, expressed as genome copies per gram of feces, is shown as colored squares. GAPDH; Glyceraldehyd-3-fosfatdehydrogenas

Article Snippet: Also, since HuTu80 cells originate from EECs, a duodenal adenocarcinoma of L-cell type (ATCC HTB-40), we investigated whether another EEC cell line, GOT1, could serve as a potential model for sapovirus infection.

Techniques: Construct, Immunofluorescence, Staining, Gene Expression, Expressing, Virus, Infection

Journal: bioRxiv

Article Title: Human enterochromaffin cells and apical-out intestinal organoids as models for human sapovirus infection

doi: 10.64898/2026.03.06.710023

Figure Lengend Snippet: Viral load for replicating and non-replicating samples in HuTu80 (left) and GOT1 (right), color coded by genotype. p-value, calculated by Mann-Whitney U test, is displayed. B) Correlation analysis of replication FC and sample viral load in HuTu80 (left) and GOT1 (right), determined by Spearman r. Left y-axis represents the fold change (FC) of virus at 72 hours post infection (hpi) compared to 2hpi. The sample viral load, displayed as genome copies per gram of stool, is shown on x-axis. Error bars show standard deviation in FC between the two biological replicates of the infection experiment. C) Replication of sapovirus GI.1, GII.1 and GII.3 in GOT1 cells in three independent experiments. y-axis shows the fold change (FC) of virus at 72 hours post infection (hpi) compared to 2hpi. The two biological duplicates per infection experiment are shown as black dots. A mean FC of at least 5-fold (dotted line) was considered successful replication. D) Replication kinetics of GI.1, GII.1 and GII.3 in GOT1 cells, presented as a fold change in viral RNA at different time points compared to at 2 hpi. Dotted line represents replication threshold of FC=5.

Article Snippet: Also, since HuTu80 cells originate from EECs, a duodenal adenocarcinoma of L-cell type (ATCC HTB-40), we investigated whether another EEC cell line, GOT1, could serve as a potential model for sapovirus infection.

Techniques: MANN-WHITNEY, Virus, Infection, Standard Deviation

Journal: Genes to Cells

Article Title: Establishment of a Novel Caco‐2‐Based Cell Culture System for Human Sapovirus Propagation

doi: 10.1111/gtc.70007

Figure Lengend Snippet: Susceptibility of various cell lines to HuSaV infection. (A) Changes in HuSaV RNA copy numbers in the culture supernatants of HuTu80 and HEK293T cells following inoculation with a HuSaV GI.1 (AH20)‐positive stool suspension (~4 × 10 7 copies/well in 96‐well plates). Each dot represents individual data points; bars indicate the geometric mean value of the HuSaV RNA copy numbers; error bars denote the geometric standard deviation (SD). This experiment was performed once with five technical replicates. (B) Changes in HuSaV RNA copy numbers in the culture supernatants of Caco‐2, HCT15, HCT116, Caco‐2/Cas9, and C2BBe1 cells following inoculation with a HuSaV GI.1 (AH20)‐positive stool suspension (~2 × 10 6 copies/well in 96‐well plates). Each dot represents individual data points; bars indicate the geometric mean HuSaV RNA copy numbers; error bars denote the geometric SD. This experiment was performed once with five technical replicates. (C) Immunofluorescence staining of the viral protein VP1 in HuTu80, HEK293T, Caco‐2, and Caco‐2/Cas9 cells at 3 dpi with a HuSaV GI.1 (AH20)‐positive stool suspension; upper panels: 4× objective lens, lower panels: 40× objective lens. (D) Flow cytometry analysis of Caco‐2 and Caco‐2/Cas9 cells infected with a HuSaV GI.1 (AH20)‐positive stool suspension at 4 dpi. Blue: VP1‐negative cells (uninfected), red: VP1‐positive cells (infected).

Article Snippet: The human duodenal cancer cell line HuTu80 was purchased from ATCC and cultured in Iscove's Modified Dulbecco's Medium (IMDM; SIGMA) supplemented with 1% GlutaMAX‐I (Gibco, Massachusetts, USA), 1% penicillin/streptomycin (Nacalai Tesque), and 5% heat‐inactivated fetal bovine serum (FBS: CAPRICORN, Ebsdorfergrund, Germany).

Techniques: Infection, Suspension, Standard Deviation, Immunofluorescence, Staining, Flow Cytometry

Journal: Genes to Cells

Article Title: Establishment of a Novel Caco‐2‐Based Cell Culture System for Human Sapovirus Propagation

doi: 10.1111/gtc.70007

Figure Lengend Snippet: Serial passage of HuSaV GI.1 in HuTu80, HEK293T, Caco‐2, and Caco‐2/Cas9 cells. HuSaV RNA copy numbers in the culture supernatants at 0 or 1 dpi (immediately after medium replacement) and 7 dpi for each passage, up to P + 5, in (A) HuTu80, (B) HEK293T, (C) Caco‐2, and (D) Caco‐2/Cas9 cells.

Article Snippet: The human duodenal cancer cell line HuTu80 was purchased from ATCC and cultured in Iscove's Modified Dulbecco's Medium (IMDM; SIGMA) supplemented with 1% GlutaMAX‐I (Gibco, Massachusetts, USA), 1% penicillin/streptomycin (Nacalai Tesque), and 5% heat‐inactivated fetal bovine serum (FBS: CAPRICORN, Ebsdorfergrund, Germany).

Techniques:

Journal: International journal of molecular sciences

Article Title: Cell Membrane-Integrated Neuroligin-1 Regulates the Anti-Inflammatory Effects of CRC Cell-Derived Exosomes.

doi: 10.3390/ijms26020503

Figure Lengend Snippet: Figure 6. M0 macrophages were treated with the exosomes of the NLG1 expressing (EXO) and NLG1 knock-downed (sh-EXO) CRC cells and the mRNA expression level (Log2 FC) of the CD206 and CD80 genes as phenotypical markers (a) and pro-inflammatory genes including IL8, IL6, IL1β, and TNFa as makers for the macrophage functionality (b), were analyzed using qRT-PCR. Data shown for qRT-PCR are the means ± standard deviation (SD).

Article Snippet: NLG1 silenced HuTu80 cell populations were generated by infection with lentiviral vectors targeting NLG1 from Origene: cat # TL3111634B (shNLGN1b) (pGFP-C-shLenti shRNA-29mer expression vector-ORIGENE, Rockville, MD, USA).

Techniques: Expressing, Quantitative RT-PCR, Standard Deviation

Journal: International journal of molecular sciences

Article Title: Cell Membrane-Integrated Neuroligin-1 Regulates the Anti-Inflammatory Effects of CRC Cell-Derived Exosomes.

doi: 10.3390/ijms26020503

Figure Lengend Snippet: Figure 5. M0 macrophages were treated with exosomes of NLG1-expressing CRC cells (HuTu80 cells), IL13+1L4 (M2-stimuli), LPS+INF-γ (M1-stimuli) for 48 h. The expression levels of CD206, and CD80 as makers for the macrophage phenotype (a), IL8, IL6, TNFa, and IL1β genes as makers for the macrophage functionality (b), were determined using qRT-PCR. Gene expression level (Log2 FC) was calculated relative to untreated M0 macrophages. Data shown are the means ± standard deviation (SD).

Article Snippet: NLG1 silenced HuTu80 cell populations were generated by infection with lentiviral vectors targeting NLG1 from Origene: cat # TL3111634B (shNLGN1b) (pGFP-C-shLenti shRNA-29mer expression vector-ORIGENE, Rockville, MD, USA).

Techniques: Expressing, Quantitative RT-PCR, Gene Expression, Standard Deviation

Journal: Russian Journal of Bioorganic Chemistry

Article Title: Hybrid Organomineral Carriers for Therapeutic Proteins

doi: 10.1134/s1068162023020231

Figure Lengend Snippet: Fig. 3. Induction of apoptosis of HuTu80 human duodenal adenocarcinoma cells by control polysaccharide gels impregnated with binase-free zeolites: (a), after 24 h; (b), after 48 h of incubation. Nonimmobilized binase was used as a positive control of the induction of apoptosis. The total number of cells in the population was taken as 100%.

Article Snippet: The line of HuTu80 human duodenal adenocarcinoma cells (ATCC, United States) was cultivated at 37°C in an atmosphere of 5% CO2 in a DMEM medium with the addition of penicillin/streptomycin (100 units/mL each) and 10% FBS to the monolayer state, after which the cells were trypsinized and seeded in 24-well plates at 2 × 105 cells per well.

Techniques: Control, Incubation, Positive Control

Journal: Russian Journal of Bioorganic Chemistry

Article Title: Hybrid Organomineral Carriers for Therapeutic Proteins

doi: 10.1134/s1068162023020231

Figure Lengend Snippet: Fig. 4. Induction of apoptosis of HuTu80 human duodenal adenocarcinoma cells by hybrid organomineral carriers loaded with binase: (a), after 24 h; (b), after 48 h of incubation. Nonimmobilized binase was used as a positive control of the induction of apoptosis. The total number of cells in the population was taken as 100%.

Article Snippet: The line of HuTu80 human duodenal adenocarcinoma cells (ATCC, United States) was cultivated at 37°C in an atmosphere of 5% CO2 in a DMEM medium with the addition of penicillin/streptomycin (100 units/mL each) and 10% FBS to the monolayer state, after which the cells were trypsinized and seeded in 24-well plates at 2 × 105 cells per well.

Techniques: Incubation, Positive Control