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15101s il 1beta r d systems baf201 xiap cell signaling technology 2042 cflip  (R&D Systems)


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    Structured Review

    R&D Systems 15101s il 1beta r d systems baf201 xiap cell signaling technology 2042 cflip
    15101s Il 1beta R D Systems Baf201 Xiap Cell Signaling Technology 2042 Cflip, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+xiap/Human+IL-1+beta%2FIL-1F2+Biotinylated+Antibody/pm39277863-509-19-21
    Average 93 stars, based on 30 article reviews
    15101s il 1beta r d systems baf201 xiap cell signaling technology 2042 cflip - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Pharmacological difference between degrader and inhibitor against oncogenic BCR-ABL kinase
    Article Snippet: Terbium-labeled streptavidin (Tb-SA) was from Cisbio (Codolet, France). .. The recombinant His-tagged human XIAP (BIR3, Asn252-Thr356, 895-XB-050) protein was purchased from R&D Systems (Minneapolis, MN, USA). ..

    Article Title: Ligand-directed covalent modification of protein
    Article Snippet: While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209). .. Homogeneous, Fluorescence Polarization, Competition Binding Assay Protocol for Affinity Assessment of Compound Binding to XIAP, cIAP-1 and ML-IAPβ BIR3 Domains 1× stocks of recombinant human XIAP; BIR3 domain (895-XB), recombinant human cIAP-1 (818-IA) or recombinant human ML-IAPβ (818-IA) from R&D Systems and Fluorescence Polarization (FP) assay tracer (Atto-647 tagged IAP inhibitor probe compound) were prepared in assay buffer consisting of 25 mM Tris-HCL, pH 7.5 (Sigma), 10 mM NaCl2 (Sigma), 1 mM DTT (Fluka) and 0.005% TritonX-100 (Pierce). .. While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209).

    Article Title: Different Degradation Mechanisms of Inhibitor of Apoptosis Proteins (IAPs) by the Specific and Nongenetic IAP-Dependent Protein Eraser (SNIPER).
    Article Snippet: Terbium-labeled streptavidin (Tb-SA) was from Cisbio (Codolet, France). .. Recombinant His-tagged human XIAP (BIR3, Asn252–Thr356, 895-XB-050) protein was purchased from R&D Systems (Minneapolis, MN, USA). ..

    Article Title: Development of protein degradation inducers of oncogenic BCR ‐ ABL protein by conjugation of ABL kinase inhibitors and IAP ligands
    Article Snippet: Terbium‐labeled streptavidin (Tb‐SA) was from Cisbio (Codolet, France). .. Recombinant His‐tagged human XIAP (BIR3, Asn252‐Thr356, 895‐XB‐050) protein was purchased from R&D Systems (Minneapolis, MN, USA). ..

    Article Title: Cell-penetrating peptide-conjugated XIAP-inhibitory cyclic hexapeptides enter into Jurkat cells and inhibit cell proliferation.
    Article Snippet: The binding experiments were performed by FCS measurement [26] using an MF20 microplate reader apparatus (Olympus, Tokyo, Japan) with a 488-nm argon laser for detection of the FITC-labelled peptide. .. For binding assays, FITC-labelled CHCPP 1 (0.2 lm) was mixed with recombinant human XIAP (BIR2 domain) (R & D Systems, Minneapolis, MN, USA), and the mixture was incubated for 30 min at room temperature. .. Samples were subsequently set in a 384-well, glass-bottomed microplate (Olympus) in the MF20 apparatus.

    Fluorescence:

    Article Title: Ligand-directed covalent modification of protein
    Article Snippet: While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209). .. Homogeneous, Fluorescence Polarization, Competition Binding Assay Protocol for Affinity Assessment of Compound Binding to XIAP, cIAP-1 and ML-IAPβ BIR3 Domains 1× stocks of recombinant human XIAP; BIR3 domain (895-XB), recombinant human cIAP-1 (818-IA) or recombinant human ML-IAPβ (818-IA) from R&D Systems and Fluorescence Polarization (FP) assay tracer (Atto-647 tagged IAP inhibitor probe compound) were prepared in assay buffer consisting of 25 mM Tris-HCL, pH 7.5 (Sigma), 10 mM NaCl2 (Sigma), 1 mM DTT (Fluka) and 0.005% TritonX-100 (Pierce). .. While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209).

    Binding Assay:

    Article Title: Ligand-directed covalent modification of protein
    Article Snippet: While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209). .. Homogeneous, Fluorescence Polarization, Competition Binding Assay Protocol for Affinity Assessment of Compound Binding to XIAP, cIAP-1 and ML-IAPβ BIR3 Domains 1× stocks of recombinant human XIAP; BIR3 domain (895-XB), recombinant human cIAP-1 (818-IA) or recombinant human ML-IAPβ (818-IA) from R&D Systems and Fluorescence Polarization (FP) assay tracer (Atto-647 tagged IAP inhibitor probe compound) were prepared in assay buffer consisting of 25 mM Tris-HCL, pH 7.5 (Sigma), 10 mM NaCl2 (Sigma), 1 mM DTT (Fluka) and 0.005% TritonX-100 (Pierce). .. While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209).

    Article Title: Cell-penetrating peptide-conjugated XIAP-inhibitory cyclic hexapeptides enter into Jurkat cells and inhibit cell proliferation.
    Article Snippet: The binding experiments were performed by FCS measurement [26] using an MF20 microplate reader apparatus (Olympus, Tokyo, Japan) with a 488-nm argon laser for detection of the FITC-labelled peptide. .. For binding assays, FITC-labelled CHCPP 1 (0.2 lm) was mixed with recombinant human XIAP (BIR2 domain) (R & D Systems, Minneapolis, MN, USA), and the mixture was incubated for 30 min at room temperature. .. Samples were subsequently set in a 384-well, glass-bottomed microplate (Olympus) in the MF20 apparatus.

    FP Assay:

    Article Title: Ligand-directed covalent modification of protein
    Article Snippet: While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209). .. Homogeneous, Fluorescence Polarization, Competition Binding Assay Protocol for Affinity Assessment of Compound Binding to XIAP, cIAP-1 and ML-IAPβ BIR3 Domains 1× stocks of recombinant human XIAP; BIR3 domain (895-XB), recombinant human cIAP-1 (818-IA) or recombinant human ML-IAPβ (818-IA) from R&D Systems and Fluorescence Polarization (FP) assay tracer (Atto-647 tagged IAP inhibitor probe compound) were prepared in assay buffer consisting of 25 mM Tris-HCL, pH 7.5 (Sigma), 10 mM NaCl2 (Sigma), 1 mM DTT (Fluka) and 0.005% TritonX-100 (Pierce). .. While IAP protein and tracer equilibrated at ambient temp in the dark (30-60 min), 100× stocks of test compound were prepared in 50% DMSO:H2O, serially diluted and spotted (0.5 □L/well) in duplicate wells of a 384-well, flat bottom, polypropylene, black assay plate (Greiner #781209).

    Ubiquitin Proteomics:

    Article Title: Smac/DIABLO Selectively Reduces the Levels of c-IAP1 and c-IAP2 but Not That of XIAP and Livin in HeLa Cells
    Article Snippet: .. This paper is available on line at http://www.jbc.org 16963 at SO U T H E R N IL L IN O IS U N IV on M arch 5, 2015 http://w w w .jbc.org/ D ow nloaded from of human c-IAP2, and residues 244–263 of human XIAP, and the monoclonal antibody against human ubiquitin were purchased from R & D Systems. .. Horseradish peroxidase-conjugated anti-GST antibody, anti-FLAG M2 antibody, and anti-c-Myc monoclonal antibody were from Sigma.

    Incubation:

    Article Title: Cell-penetrating peptide-conjugated XIAP-inhibitory cyclic hexapeptides enter into Jurkat cells and inhibit cell proliferation.
    Article Snippet: The binding experiments were performed by FCS measurement [26] using an MF20 microplate reader apparatus (Olympus, Tokyo, Japan) with a 488-nm argon laser for detection of the FITC-labelled peptide. .. For binding assays, FITC-labelled CHCPP 1 (0.2 lm) was mixed with recombinant human XIAP (BIR2 domain) (R & D Systems, Minneapolis, MN, USA), and the mixture was incubated for 30 min at room temperature. .. Samples were subsequently set in a 384-well, glass-bottomed microplate (Olympus) in the MF20 apparatus.



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    Image Search Results


    The effects of CM on HCM subjected to H/R-induced injury according to the XIAP expression profile. Immunofluorescence images of HCM stained with XIAP antibody (red) and DAPI (blue) after being treated with different CM following H/R-induced injury (10× magnification; 200 µm scale bar; n = 3). The bar graph represents the MFI measurement for XIAP in H/R-injured HCM after ischemic rescue with CM. Treatment with different EF–HAM CM did not upregulate the expression of XIAP injured HCM. The values are represented as mean ± SEM ( n = 3; * p < 0.05 or ** p < 0.01, compared with Normoxic control; one-way ANOVA).

    Journal: International Journal of Molecular Sciences

    Article Title: Ischemic Rescue Potential of Conditioned Medium Derived from Skeletal Muscle Cells-Seeded Electrospun Fiber-Coated Human Amniotic Membrane Scaffolds

    doi: 10.3390/ijms252111697

    Figure Lengend Snippet: The effects of CM on HCM subjected to H/R-induced injury according to the XIAP expression profile. Immunofluorescence images of HCM stained with XIAP antibody (red) and DAPI (blue) after being treated with different CM following H/R-induced injury (10× magnification; 200 µm scale bar; n = 3). The bar graph represents the MFI measurement for XIAP in H/R-injured HCM after ischemic rescue with CM. Treatment with different EF–HAM CM did not upregulate the expression of XIAP injured HCM. The values are represented as mean ± SEM ( n = 3; * p < 0.05 or ** p < 0.01, compared with Normoxic control; one-way ANOVA).

    Article Snippet: The HCMs were incubated with mouse monoclonal anti-human XIAP (A-7) antibody (1:50 dilution; Santa Cruz Biotechnology, Dallas, TX, USA), followed by immunolabeling by goat anti-mouse Alexa Fluor ® 594 secondary antibody (1:300 dilution) and counterstaining with DAPI.

    Techniques: Expressing, Immunofluorescence, Staining, Control