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trail neutralizing antibody  (R&D Systems)


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    Structured Review

    R&D Systems trail neutralizing antibody
    Trail Neutralizing Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+trail/Human+TRAIL%2FTNFSF10+Antibody/pmc13111737-305-11-15
    Average 94 stars, based on 27 article reviews
    trail neutralizing antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Noncompetitive Allosteric Antagonism of Death Receptor 5 by a Synthetic Affibody Ligand.
    Article Snippet: .. Huh-7 cells grown in 96-well plates were treated with recombinant human TRAIL (20 ng/mL, R&D Systems, 375-TL-010) for 16 h in the presence or absence of increasing concentrations of ABYDR5−6 (78 nM to 10 μM). .. Palmitate was dissolved in isopropyl alcohol and conjugated to bovine serum albumin resuspended in DMEM (1% bovine serum albumin) as previously described.49 Following the incubation period, cells were stained with cell permeable Hoechst 33342 (Invitrogen, H1399) and live cell-impermeant SYTOX Green (Invitrogen, S7020) to label all and dead cells, respectively.

    Article Title: Circulating Tumor Cells Develop Resistance to TRAIL-Induced Apoptosis Through Autophagic Removal of Death Receptor 5: Evidence from an In Vitro Model
    Article Snippet: .. The cells were treated with the recombinant human TRAIL (TRAIL) (R&D Systems, 375-TEC), at two different doses (10 ng/mL and 100 ng/mL for MDA-MB-231 and ZR75-1 cell lines; 50 ng/mL and 500 ng/mL for MCF7 cell lines). ..

    Article Title: TRAIL-Based Therapies Efficacy in Pediatric Bone Tumors Models Is Modulated by TRAIL Non-Apoptotic Pathway Activation via RIPK1 Recruitment.
    Article Snippet: TC-71 cells were cultured in Rosewell Park Millenium Institute (RPMI, Lonza) medium with 5% FBS and 2 mmol/L L-glutamine. .. Two thousand cells per well were seeded into 96-well plates and cultured for 72 h in the presence of increasing concentrations of recombinant human TRAIL (R&D systems, Minneapolis, USA), AMG655 (Amgen, Thousand Oaks, CA, USA) or APG880 (Apogenix, Heidelberg, Germany) as indicated. .. Cell viability was determined by Cristal Violet staining (Sigma-Aldrich, St. Louis, MO, USA) as previously described [33].

    Article Title: In vitro and in vivo discrepancy in inducing apoptosis by mesenchymal stromal cells delivering membrane-bound tumor necrosis factor-related apoptosis inducing ligand in osteosarcoma pre-clinical models.
    Article Snippet: Background: Osteosarcoma (OS) is the most frequent pediatric malignant bone tumor.. OS patients have not seen any major therapeutic progress in the last 30 years, in particular in the case of metastatic disease, which requires new therapeutic strategies.. The pro-apoptotic cytokine Tumor necrosis factor (TNF) Related Apoptosis Inducing Ligand (TRAIL) can selectively kill tumor cells while sparing normal cells, making it a promising therapeutic tool in several types of cancer.

    Article Title: Identification of a marine-derived sesquiterpenoid, Compound-8, that inhibits tumour necrosis factor-induced cell death by blocking complex II assembly.
    Article Snippet: Tongji University Cancer Center, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China Tongji University School of Medicine, Shanghai, China School of Pharmacy, Second Military Medical University, Shanghai, China School of Pharmacy, Nanchang University, Nanchang, China Shanghai Power Hospital, Shanghai, China Department of ORL-HNS, Shanghai Fourth People's Hospital, Tongji University School of Medicine, Shanghai, China The Royal College of Surgeons in Ireland, Dublin, Ireland The Royal College of Surgeons of England, London, UK Center of Excellence for Cancer and Inflammation, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand Ningbo Institute of Marine Medicine, Peking University, Beijing, China

    Multiple Displacement Amplification:

    Article Title: Circulating Tumor Cells Develop Resistance to TRAIL-Induced Apoptosis Through Autophagic Removal of Death Receptor 5: Evidence from an In Vitro Model
    Article Snippet: .. The cells were treated with the recombinant human TRAIL (TRAIL) (R&D Systems, 375-TEC), at two different doses (10 ng/mL and 100 ng/mL for MDA-MB-231 and ZR75-1 cell lines; 50 ng/mL and 500 ng/mL for MCF7 cell lines). ..

    Cell Culture:

    Article Title: TRAIL-Based Therapies Efficacy in Pediatric Bone Tumors Models Is Modulated by TRAIL Non-Apoptotic Pathway Activation via RIPK1 Recruitment.
    Article Snippet: TC-71 cells were cultured in Rosewell Park Millenium Institute (RPMI, Lonza) medium with 5% FBS and 2 mmol/L L-glutamine. .. Two thousand cells per well were seeded into 96-well plates and cultured for 72 h in the presence of increasing concentrations of recombinant human TRAIL (R&D systems, Minneapolis, USA), AMG655 (Amgen, Thousand Oaks, CA, USA) or APG880 (Apogenix, Heidelberg, Germany) as indicated. .. Cell viability was determined by Cristal Violet staining (Sigma-Aldrich, St. Louis, MO, USA) as previously described [33].

    Article Title: In vitro and in vivo discrepancy in inducing apoptosis by mesenchymal stromal cells delivering membrane-bound tumor necrosis factor-related apoptosis inducing ligand in osteosarcoma pre-clinical models.
    Article Snippet: Background: Osteosarcoma (OS) is the most frequent pediatric malignant bone tumor.. OS patients have not seen any major therapeutic progress in the last 30 years, in particular in the case of metastatic disease, which requires new therapeutic strategies.. The pro-apoptotic cytokine Tumor necrosis factor (TNF) Related Apoptosis Inducing Ligand (TRAIL) can selectively kill tumor cells while sparing normal cells, making it a promising therapeutic tool in several types of cancer.

    Incubation:

    Article Title: Preclinical studies of a death receptor 5 fusion protein that ameliorates acute liver failure.
    Article Snippet: Acute liver failure (ALF) is a life-threatening disease with a high mortality rate.. There is an urgent need to develop new drugs with high efficacy and low toxicity.. In this study, we produced a pharmaceutical-grade soluble death receptor 5 (sDR5)-Fc fusion protein for treating ALF and evaluated the pharmacology, safety, pharmacokinetics, efficacy, and mechanisms of sDR5-Fc in mice, rats, and cynomolgus monkeys. sDR5-Fc bound with high affinity to both human and monkey tumor necrosis factorrelated apoptosis-inducing ligand (TRAIL) effectively blocked TRAIL-induced apoptosis in vitro and significantly ameliorated ALF induced by concanavalin A (Con A) in mice.



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    (A) Luciferase-based cytotoxicity assay to validate the impact of selected HITs on FluTC-mediated MO3.13-A2-Luc killing. MO3.13-A2-Luc cells were transfected with IRG-specific pool of 30 siRNA (siTOOLs) and co-cultured with FluTC as described in . Graph indicates cytotoxicity/viability ratio normalized to Scr control. (B-C) Luminex-based cytokine analysis of the FluTC – MO3.13-A2-Luc cell co-cultures. MO3.13-A2-Luc cells transfected either with Scr siRNA or IRG-specific siRNA pool and (B) co-cultured with FluTC for 24 h or (C) cultured in CM. (B) MCP-1 and (C) IL-8 levels were depicted. (B) Each line represents an independent experiment; values indicate the average of (B) triplicates or (C) duplicates. (A) Representative data of at least 2 independent experiments with triplicates per sample. (A-C) Graphs show mean +/- SD. P-values were calculated using (A) two-tailed student’s t-test (B) Ratio-paired t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001. (D) Cytokine analysis to determine IFNγ, TNFα, FasL and <t>TRAIL</t> secretion by anti-CD3/CD28 activated FluTC. Values represent the mean ± SD of triplicates.
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    (A) Luciferase-based cytotoxicity assay to validate the impact of selected HITs on FluTC-mediated MO3.13-A2-Luc killing. MO3.13-A2-Luc cells were transfected with IRG-specific pool of 30 siRNA (siTOOLs) and co-cultured with FluTC as described in . Graph indicates cytotoxicity/viability ratio normalized to Scr control. (B-C) Luminex-based cytokine analysis of the FluTC – MO3.13-A2-Luc cell co-cultures. MO3.13-A2-Luc cells transfected either with Scr siRNA or IRG-specific siRNA pool and (B) co-cultured with FluTC for 24 h or (C) cultured in CM. (B) MCP-1 and (C) IL-8 levels were depicted. (B) Each line represents an independent experiment; values indicate the average of (B) triplicates or (C) duplicates. (A) Representative data of at least 2 independent experiments with triplicates per sample. (A-C) Graphs show mean +/- SD. P-values were calculated using (A) two-tailed student’s t-test (B) Ratio-paired t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001. (D) Cytokine analysis to determine IFNγ, TNFα, FasL and TRAIL secretion by anti-CD3/CD28 activated FluTC. Values represent the mean ± SD of triplicates.

    Journal: bioRxiv

    Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets

    doi: 10.64898/2026.04.21.719872

    Figure Lengend Snippet: (A) Luciferase-based cytotoxicity assay to validate the impact of selected HITs on FluTC-mediated MO3.13-A2-Luc killing. MO3.13-A2-Luc cells were transfected with IRG-specific pool of 30 siRNA (siTOOLs) and co-cultured with FluTC as described in . Graph indicates cytotoxicity/viability ratio normalized to Scr control. (B-C) Luminex-based cytokine analysis of the FluTC – MO3.13-A2-Luc cell co-cultures. MO3.13-A2-Luc cells transfected either with Scr siRNA or IRG-specific siRNA pool and (B) co-cultured with FluTC for 24 h or (C) cultured in CM. (B) MCP-1 and (C) IL-8 levels were depicted. (B) Each line represents an independent experiment; values indicate the average of (B) triplicates or (C) duplicates. (A) Representative data of at least 2 independent experiments with triplicates per sample. (A-C) Graphs show mean +/- SD. P-values were calculated using (A) two-tailed student’s t-test (B) Ratio-paired t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001. (D) Cytokine analysis to determine IFNγ, TNFα, FasL and TRAIL secretion by anti-CD3/CD28 activated FluTC. Values represent the mean ± SD of triplicates.

    Article Snippet: Supernatants of FluTC-MO3.13-A2-Luc co-cultures and anti-CD3/CD28 activated FluTC were further analyzed for the detection of IFNγ (Human IFN-γ ELISA Set, BD OptEIA, #555142), TNFα (Human TNF ELISA Set, BD OptEIA, #555212), Granzyme B (Human Granzyme B ELISA development kit, Mabtech, #3485-1H-20) and TRAIL (human TRAIL/TNFSF10 DuoSet ELISA, R&D Systems, #DY375-05) ( , ).

    Techniques: Luciferase, Cytotoxicity Assay, Transfection, Cell Culture, Control, Luminex, Two Tailed Test

    (A) FACS analysis to determine the surface expression of IFNG-R1, TNF-R1, TNF-R2, FAS, TRAIL-R1, and TRAIL-R2 in MO3.13-A2-Luc cells. Grey histograms represent the isotype control. (B) Impact of IRG knockdown on TRAIL-R2 surface expression in MO3.13-A2-Luc cells. Left panel: Representative overlay of histograms to compare TRAIL-R2 expression on IRG +/- MO3.13-A2-Luc cells. Right panel: Compiled data of mean fluorescence intensity (MFI) for TRAIL-R2 expression. (C) Real-time cytotoxicity assay (IncuCyte® SX5 System) to analyze TRAIL-induced apoptosis over 48 hours in IRG +/- MO3.13-A2-Luc cells. Incucyte® Cytotox-Red Dye was added to the transfected MO3.13 oligodendrocytes together with TRAIL treatment as an indicator of apoptosis. The graph shows total red object integrated intensity per well (RCU x µm²/Image). (D) Western blot analysis of total/cleaved caspase-3/8/9, TRAF2, phosho(p)/total TAK1, MKK4, JNK, NF-κB, pLKB1 and Bcl2 in IRG +/- MO3.13-A2-Luc cells upon 4 h treatment with TRAIL. Experiment was run in two separate blots each including Scr samples. Each HIT was shown in comparison to Scr sample analyzed in the same blot (Blot1: Scr, siSTK11, siKCNH8 and siABCA2; Blot2: Scr, siSLC1A3 and siCHRNA1). Representative data of (A & D) two, (B-C) three independent experiments. Values represent the mean ± SD. P-value was calculated using paired two-tailed Student’s t-test (* = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001).

    Journal: bioRxiv

    Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets

    doi: 10.64898/2026.04.21.719872

    Figure Lengend Snippet: (A) FACS analysis to determine the surface expression of IFNG-R1, TNF-R1, TNF-R2, FAS, TRAIL-R1, and TRAIL-R2 in MO3.13-A2-Luc cells. Grey histograms represent the isotype control. (B) Impact of IRG knockdown on TRAIL-R2 surface expression in MO3.13-A2-Luc cells. Left panel: Representative overlay of histograms to compare TRAIL-R2 expression on IRG +/- MO3.13-A2-Luc cells. Right panel: Compiled data of mean fluorescence intensity (MFI) for TRAIL-R2 expression. (C) Real-time cytotoxicity assay (IncuCyte® SX5 System) to analyze TRAIL-induced apoptosis over 48 hours in IRG +/- MO3.13-A2-Luc cells. Incucyte® Cytotox-Red Dye was added to the transfected MO3.13 oligodendrocytes together with TRAIL treatment as an indicator of apoptosis. The graph shows total red object integrated intensity per well (RCU x µm²/Image). (D) Western blot analysis of total/cleaved caspase-3/8/9, TRAF2, phosho(p)/total TAK1, MKK4, JNK, NF-κB, pLKB1 and Bcl2 in IRG +/- MO3.13-A2-Luc cells upon 4 h treatment with TRAIL. Experiment was run in two separate blots each including Scr samples. Each HIT was shown in comparison to Scr sample analyzed in the same blot (Blot1: Scr, siSTK11, siKCNH8 and siABCA2; Blot2: Scr, siSLC1A3 and siCHRNA1). Representative data of (A & D) two, (B-C) three independent experiments. Values represent the mean ± SD. P-value was calculated using paired two-tailed Student’s t-test (* = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001).

    Article Snippet: Supernatants of FluTC-MO3.13-A2-Luc co-cultures and anti-CD3/CD28 activated FluTC were further analyzed for the detection of IFNγ (Human IFN-γ ELISA Set, BD OptEIA, #555142), TNFα (Human TNF ELISA Set, BD OptEIA, #555212), Granzyme B (Human Granzyme B ELISA development kit, Mabtech, #3485-1H-20) and TRAIL (human TRAIL/TNFSF10 DuoSet ELISA, R&D Systems, #DY375-05) ( , ).

    Techniques: Expressing, Control, Knockdown, Fluorescence, Cytotoxicity Assay, Transfection, Western Blot, Comparison, Two Tailed Test

    (A-B) Impact of SIK3 on TRAIL-mediated MO3.13-A2-Luc killing was determined by (A) Luciferase-based cytotoxicity assay as described in supp and (B) real-time cytotoxicity assay as described in . Representative data of (A) three (B) two independent experiments. Values represent the mean ± SD. P-value was calculated using paired two-tailed Student’s t-test (* = p < 0.05, ** = p < 0.01).

    Journal: bioRxiv

    Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets

    doi: 10.64898/2026.04.21.719872

    Figure Lengend Snippet: (A-B) Impact of SIK3 on TRAIL-mediated MO3.13-A2-Luc killing was determined by (A) Luciferase-based cytotoxicity assay as described in supp and (B) real-time cytotoxicity assay as described in . Representative data of (A) three (B) two independent experiments. Values represent the mean ± SD. P-value was calculated using paired two-tailed Student’s t-test (* = p < 0.05, ** = p < 0.01).

    Article Snippet: Supernatants of FluTC-MO3.13-A2-Luc co-cultures and anti-CD3/CD28 activated FluTC were further analyzed for the detection of IFNγ (Human IFN-γ ELISA Set, BD OptEIA, #555142), TNFα (Human TNF ELISA Set, BD OptEIA, #555212), Granzyme B (Human Granzyme B ELISA development kit, Mabtech, #3485-1H-20) and TRAIL (human TRAIL/TNFSF10 DuoSet ELISA, R&D Systems, #DY375-05) ( , ).

    Techniques: Luciferase, Cytotoxicity Assay, Two Tailed Test

    Summary of molecular pathways involved in the downstream signaling of STK11/LKB1, KCNH8, ABCA2, SLC1A3/EAAT1 and/or CHRNA1/AChR-mediated immune resistance. Activation of caspase-3/-8/-9, MKK4, JNK1, NF-κB and expression of TRAF2, TRAIL-R2, IL-8 was experimentally depicted in this study, whereas the potential involvement of the other molecular components was deducted from the previous studies as described and referred in the results and discussion sections above. Colors of the lines depicting the regulatory networks are matched with colors of the IRG icons. Activating and inhibitory effects are indicated by arrows and blocked lines respectively. The figure was generated using biorender.com (Created in BioRender. Beckhove, P. (2026) https://BioRender.com/2adzain )

    Journal: bioRxiv

    Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets

    doi: 10.64898/2026.04.21.719872

    Figure Lengend Snippet: Summary of molecular pathways involved in the downstream signaling of STK11/LKB1, KCNH8, ABCA2, SLC1A3/EAAT1 and/or CHRNA1/AChR-mediated immune resistance. Activation of caspase-3/-8/-9, MKK4, JNK1, NF-κB and expression of TRAF2, TRAIL-R2, IL-8 was experimentally depicted in this study, whereas the potential involvement of the other molecular components was deducted from the previous studies as described and referred in the results and discussion sections above. Colors of the lines depicting the regulatory networks are matched with colors of the IRG icons. Activating and inhibitory effects are indicated by arrows and blocked lines respectively. The figure was generated using biorender.com (Created in BioRender. Beckhove, P. (2026) https://BioRender.com/2adzain )

    Article Snippet: Supernatants of FluTC-MO3.13-A2-Luc co-cultures and anti-CD3/CD28 activated FluTC were further analyzed for the detection of IFNγ (Human IFN-γ ELISA Set, BD OptEIA, #555142), TNFα (Human TNF ELISA Set, BD OptEIA, #555212), Granzyme B (Human Granzyme B ELISA development kit, Mabtech, #3485-1H-20) and TRAIL (human TRAIL/TNFSF10 DuoSet ELISA, R&D Systems, #DY375-05) ( , ).

    Techniques: Activation Assay, Expressing, Generated