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human starr seq vector  (TaKaRa)


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    TaKaRa human starr seq vector
    Human Starr Seq Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+starr+seq+vector/Sal+I/pmc08053126-49-9-29
    Average 96 stars, based on 3342 article reviews
    human starr seq vector - by Bioz Stars, 2026-09
    96/100 stars

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    Construct:

    Article Title: Androgen and glucocorticoid receptor direct distinct transcriptional programs by receptor-specific and shared DNA binding sites
    Article Snippet: .. Individual STARR reporter constructs were generated by digesting the human STARR-seq vector ( ) with SalI and AgeI and subsequent insertion of fragments of interest by In-Fusion HD cloning (TaKaRa). .. Fragments of interest: positive control region (near IP6K3 gene, hg19: chr6:33 698 504–33 698 853), AQP3 enhancer (hg19: chr9:33 437 258–33 437 811) and the AQP3 enhancer with AR binding site mutations ( AQP3 -Deleted and AQP3 -AGA → TGT) were ordered as a gBlock (IDT) or GeneStrand (Eurofins) (see for the sequence of regions).

    Article Title: Synthetic STARR-seq reveals how DNA shape and sequence modulate transcriptional output and noise
    Article Snippet: .. STARR reporter constructs were generated by digesting the human STARR-seq vector [ ] with SalI-HF and AgeI-HF and subsequent insertion of fragments of interest by in-Fusion HD cloning (TaKaRa). ..

    Generated:

    Article Title: Androgen and glucocorticoid receptor direct distinct transcriptional programs by receptor-specific and shared DNA binding sites
    Article Snippet: .. Individual STARR reporter constructs were generated by digesting the human STARR-seq vector ( ) with SalI and AgeI and subsequent insertion of fragments of interest by In-Fusion HD cloning (TaKaRa). .. Fragments of interest: positive control region (near IP6K3 gene, hg19: chr6:33 698 504–33 698 853), AQP3 enhancer (hg19: chr9:33 437 258–33 437 811) and the AQP3 enhancer with AR binding site mutations ( AQP3 -Deleted and AQP3 -AGA → TGT) were ordered as a gBlock (IDT) or GeneStrand (Eurofins) (see for the sequence of regions).

    Article Title: Synthetic STARR-seq reveals how DNA shape and sequence modulate transcriptional output and noise
    Article Snippet: .. STARR reporter constructs were generated by digesting the human STARR-seq vector [ ] with SalI-HF and AgeI-HF and subsequent insertion of fragments of interest by in-Fusion HD cloning (TaKaRa). ..

    Plasmid Preparation:

    Article Title: Androgen and glucocorticoid receptor direct distinct transcriptional programs by receptor-specific and shared DNA binding sites
    Article Snippet: .. Individual STARR reporter constructs were generated by digesting the human STARR-seq vector ( ) with SalI and AgeI and subsequent insertion of fragments of interest by In-Fusion HD cloning (TaKaRa). .. Fragments of interest: positive control region (near IP6K3 gene, hg19: chr6:33 698 504–33 698 853), AQP3 enhancer (hg19: chr9:33 437 258–33 437 811) and the AQP3 enhancer with AR binding site mutations ( AQP3 -Deleted and AQP3 -AGA → TGT) were ordered as a gBlock (IDT) or GeneStrand (Eurofins) (see for the sequence of regions).

    Article Title: Synthetic STARR-seq reveals how DNA shape and sequence modulate transcriptional output and noise
    Article Snippet: .. STARR reporter constructs were generated by digesting the human STARR-seq vector [ ] with SalI-HF and AgeI-HF and subsequent insertion of fragments of interest by in-Fusion HD cloning (TaKaRa). ..

    Cloning:

    Article Title: Androgen and glucocorticoid receptor direct distinct transcriptional programs by receptor-specific and shared DNA binding sites
    Article Snippet: .. Individual STARR reporter constructs were generated by digesting the human STARR-seq vector ( ) with SalI and AgeI and subsequent insertion of fragments of interest by In-Fusion HD cloning (TaKaRa). .. Fragments of interest: positive control region (near IP6K3 gene, hg19: chr6:33 698 504–33 698 853), AQP3 enhancer (hg19: chr9:33 437 258–33 437 811) and the AQP3 enhancer with AR binding site mutations ( AQP3 -Deleted and AQP3 -AGA → TGT) were ordered as a gBlock (IDT) or GeneStrand (Eurofins) (see for the sequence of regions).

    Article Title: Synthetic STARR-seq reveals how DNA shape and sequence modulate transcriptional output and noise
    Article Snippet: .. STARR reporter constructs were generated by digesting the human STARR-seq vector [ ] with SalI-HF and AgeI-HF and subsequent insertion of fragments of interest by in-Fusion HD cloning (TaKaRa). ..



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    (A-E): Schematic representation of a <t>STARR-seq</t> protocol highlighting all sequence information. Schematic of STARR-seq experimental protocol and illustrating complete sequence information is shown. (A) Insert preparation including addition of sequencing adapters and cloning arms to library fragments to facilitate sequencing and library cloning. (B) STARR-seq plasmid library preparation including <t>vector</t> linearization and cloning of library inserts into <t>human</t> STARR-seq vector followed by library amplification through transformation. (C) Preparation of ‘input’ sequencing library either directly from plasmid library or from DNA extracted from library-transfected host cells. This step adds on UMIs and index barcodes to the library prior to sequencing to sort for PCR duplicates and to sequence multiple libraries on the same sequencing lane (multiplexing). (D) cDNA library generation for STARR-seq screening includes transfection of the plasmid library into a host cell and reverse transcription of self-transcribed reporter transcripts. This step also involves adding UMIs for detecting and removing PCR duplicates. (E) Preparation of ‘output’ sequencing library involves adding sequencing barcode indexes to the screening library prior to sequencing to enable multiplexing. Both ‘input’ and ‘output’ libraries are pooled and sequenced in parallel for enhancer screening.
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    (A-E): Schematic representation of a <t>STARR-seq</t> protocol highlighting all sequence information. Schematic of STARR-seq experimental protocol and illustrating complete sequence information is shown. (A) Insert preparation including addition of sequencing adapters and cloning arms to library fragments to facilitate sequencing and library cloning. (B) STARR-seq plasmid library preparation including <t>vector</t> linearization and cloning of library inserts into <t>human</t> STARR-seq vector followed by library amplification through transformation. (C) Preparation of ‘input’ sequencing library either directly from plasmid library or from DNA extracted from library-transfected host cells. This step adds on UMIs and index barcodes to the library prior to sequencing to sort for PCR duplicates and to sequence multiple libraries on the same sequencing lane (multiplexing). (D) cDNA library generation for STARR-seq screening includes transfection of the plasmid library into a host cell and reverse transcription of self-transcribed reporter transcripts. This step also involves adding UMIs for detecting and removing PCR duplicates. (E) Preparation of ‘output’ sequencing library involves adding sequencing barcode indexes to the screening library prior to sequencing to enable multiplexing. Both ‘input’ and ‘output’ libraries are pooled and sequenced in parallel for enhancer screening.
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    Image Search Results


    (A-E): Schematic representation of a STARR-seq protocol highlighting all sequence information. Schematic of STARR-seq experimental protocol and illustrating complete sequence information is shown. (A) Insert preparation including addition of sequencing adapters and cloning arms to library fragments to facilitate sequencing and library cloning. (B) STARR-seq plasmid library preparation including vector linearization and cloning of library inserts into human STARR-seq vector followed by library amplification through transformation. (C) Preparation of ‘input’ sequencing library either directly from plasmid library or from DNA extracted from library-transfected host cells. This step adds on UMIs and index barcodes to the library prior to sequencing to sort for PCR duplicates and to sequence multiple libraries on the same sequencing lane (multiplexing). (D) cDNA library generation for STARR-seq screening includes transfection of the plasmid library into a host cell and reverse transcription of self-transcribed reporter transcripts. This step also involves adding UMIs for detecting and removing PCR duplicates. (E) Preparation of ‘output’ sequencing library involves adding sequencing barcode indexes to the screening library prior to sequencing to enable multiplexing. Both ‘input’ and ‘output’ libraries are pooled and sequenced in parallel for enhancer screening.

    Journal: bioRxiv

    Article Title: Challenges and considerations for reproducibility of STARR-seq assays

    doi: 10.1101/2022.07.27.501795

    Figure Lengend Snippet: (A-E): Schematic representation of a STARR-seq protocol highlighting all sequence information. Schematic of STARR-seq experimental protocol and illustrating complete sequence information is shown. (A) Insert preparation including addition of sequencing adapters and cloning arms to library fragments to facilitate sequencing and library cloning. (B) STARR-seq plasmid library preparation including vector linearization and cloning of library inserts into human STARR-seq vector followed by library amplification through transformation. (C) Preparation of ‘input’ sequencing library either directly from plasmid library or from DNA extracted from library-transfected host cells. This step adds on UMIs and index barcodes to the library prior to sequencing to sort for PCR duplicates and to sequence multiple libraries on the same sequencing lane (multiplexing). (D) cDNA library generation for STARR-seq screening includes transfection of the plasmid library into a host cell and reverse transcription of self-transcribed reporter transcripts. This step also involves adding UMIs for detecting and removing PCR duplicates. (E) Preparation of ‘output’ sequencing library involves adding sequencing barcode indexes to the screening library prior to sequencing to enable multiplexing. Both ‘input’ and ‘output’ libraries are pooled and sequenced in parallel for enhancer screening.

    Article Snippet: Use human STARR-seq vector ADDgene #99296 as reported by Muerdter and colleagues .

    Techniques: Sequencing, Clone Assay, Plasmid Preparation, Amplification, Transformation Assay, Transfection, Multiplexing, cDNA Library Assay