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primary gingival fibroblast; normal, human, adult  (ATCC)


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    ATCC primary gingival fibroblast; normal, human, adult
    Primary Gingival Fibroblast; Normal, Human, Adult, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+primary+fibroblasts/Primary+Gingival+Fibroblast%3B+Normal%2C+Human%2C+Adult/custom%40pcs-201-018%4041918333
    Average 99 stars, based on 517 article reviews
    primary gingival fibroblast; normal, human, adult - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Mechanistic insights into the activity of Benzoxaboroles against Cryptosporidium parvum : CPSF3 targeting and resistance implications
    Article Snippet: Human ileocecal adenocarcinoma cells (HCT-8 cells; ATCC CCL-34) were cultured in RPMI 1640 medium supplemented with glutamine (Gibco), 10 % fetal bovine serum (Dutscher), 50U/ml penicillin, 50 μg/ml streptomycin (Gibco), 15 mM HEPES (Gibco, HEPES Buffer Solution 1 M), and 1 x Sodium pyruvate (Gibco, Sodium pyruvate 100 mM, 100). .. Human primary fibroblasts (HFFs, ATCC® CCL-171TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS) (Invitrogen), 10 mM (4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid) (HEPES) buffer pH 7.2, 2 mM L-glutamine and 50 μg/ml of penicillin and streptomycin (Invitrogen). .. CLEC213 chicken epithelial cells were cultured in Dulbecco’s modified Eagle’s medium F12 (DMEM F12) medium supplemented with 10% fetal bovine serum (FBS, Dutscher), penicillin (50 U/ml, Cytiva, Hyclone), and streptomycin (50 μg/ml, Cytiva, Hyclone).

    Article Title: Structural and functional characterization of Tg GSK3, a druggable kinase in Toxoplasma gondii
    Article Snippet: .. Human primary fibroblasts (HFFs, ATCC® CCL-171TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Invitrogen), 10 mM (4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid) (HEPES) buffer pH 7.2, 2 mM L-glutamine and 50 μg/ml penicillin and streptomycin (Invitrogen). ..

    Article Title: Uncovering biomarkers for chronic toxoplasmosis detection highlights alternative pathways shaping parasite dormancy
    Article Snippet: .. Human primary fibroblasts (HFFs, ATCC® CCL-171TM) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Invitrogen), 10 mM (4-(2-hydroxyethyl)-1-piperazine ethane sulphonic acid) (HEPES) buffer pH 7.2, 2 mM l -glutamine, and 50 μg/mL of penicillin and streptomycin (Invitrogen). ..

    Modification:

    Article Title: Mechanistic insights into the activity of Benzoxaboroles against Cryptosporidium parvum : CPSF3 targeting and resistance implications
    Article Snippet: Human ileocecal adenocarcinoma cells (HCT-8 cells; ATCC CCL-34) were cultured in RPMI 1640 medium supplemented with glutamine (Gibco), 10 % fetal bovine serum (Dutscher), 50U/ml penicillin, 50 μg/ml streptomycin (Gibco), 15 mM HEPES (Gibco, HEPES Buffer Solution 1 M), and 1 x Sodium pyruvate (Gibco, Sodium pyruvate 100 mM, 100). .. Human primary fibroblasts (HFFs, ATCC® CCL-171TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS) (Invitrogen), 10 mM (4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid) (HEPES) buffer pH 7.2, 2 mM L-glutamine and 50 μg/ml of penicillin and streptomycin (Invitrogen). .. CLEC213 chicken epithelial cells were cultured in Dulbecco’s modified Eagle’s medium F12 (DMEM F12) medium supplemented with 10% fetal bovine serum (FBS, Dutscher), penicillin (50 U/ml, Cytiva, Hyclone), and streptomycin (50 μg/ml, Cytiva, Hyclone).

    Article Title: Structural and functional characterization of Tg GSK3, a druggable kinase in Toxoplasma gondii
    Article Snippet: .. Human primary fibroblasts (HFFs, ATCC® CCL-171TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Invitrogen), 10 mM (4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid) (HEPES) buffer pH 7.2, 2 mM L-glutamine and 50 μg/ml penicillin and streptomycin (Invitrogen). ..

    Article Title: Uncovering biomarkers for chronic toxoplasmosis detection highlights alternative pathways shaping parasite dormancy
    Article Snippet: .. Human primary fibroblasts (HFFs, ATCC® CCL-171TM) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Invitrogen), 10 mM (4-(2-hydroxyethyl)-1-piperazine ethane sulphonic acid) (HEPES) buffer pH 7.2, 2 mM l -glutamine, and 50 μg/mL of penicillin and streptomycin (Invitrogen). ..



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    A) Hydrogels were composed of either 0% or 50% mixture of tyramine-modified silk and were exposed to media without or with 5 ng/mL TGFβ. After hydrogels were cast, 25,000 <t>NHLF</t> cells were seeded on the surface. B) SEM imaging of the surface of the silk hydrogels incubated without cells for Day 0 and Day 14. C) Live/Dead imaging of Calcein AM for live cells (green) and Ethidium Homodimer-1 for dead cells (red) indicated cytocompatibility. D) Alamar Blue Assay for metabolic activity indicated that 0% gels showed an increase in activity over 14 days, while 50% gels plateaued from day 7 to 14. E) Mammalian β-Galactosidase Assay for senescence revealed no change for any timepoints tested over 14 days. N=3-4, Error bars = SEM.TCP = Tissue Culture Plate, T-= without TGFβ, T+ = 5 ng/mL of TGFβ. Two-way ANOVA, Tukey’s: ns = not significant p > 0.05, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.
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    Image Search Results


    A) Hydrogels were composed of either 0% or 50% mixture of tyramine-modified silk and were exposed to media without or with 5 ng/mL TGFβ. After hydrogels were cast, 25,000 NHLF cells were seeded on the surface. B) SEM imaging of the surface of the silk hydrogels incubated without cells for Day 0 and Day 14. C) Live/Dead imaging of Calcein AM for live cells (green) and Ethidium Homodimer-1 for dead cells (red) indicated cytocompatibility. D) Alamar Blue Assay for metabolic activity indicated that 0% gels showed an increase in activity over 14 days, while 50% gels plateaued from day 7 to 14. E) Mammalian β-Galactosidase Assay for senescence revealed no change for any timepoints tested over 14 days. N=3-4, Error bars = SEM.TCP = Tissue Culture Plate, T-= without TGFβ, T+ = 5 ng/mL of TGFβ. Two-way ANOVA, Tukey’s: ns = not significant p > 0.05, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.

    Journal: bioRxiv

    Article Title: Progressive matrix stiffening of tyramine-modified silk fibroin hydrogels governs stage-specific pulmonary fibroblast activation

    doi: 10.64898/2026.06.01.729382

    Figure Lengend Snippet: A) Hydrogels were composed of either 0% or 50% mixture of tyramine-modified silk and were exposed to media without or with 5 ng/mL TGFβ. After hydrogels were cast, 25,000 NHLF cells were seeded on the surface. B) SEM imaging of the surface of the silk hydrogels incubated without cells for Day 0 and Day 14. C) Live/Dead imaging of Calcein AM for live cells (green) and Ethidium Homodimer-1 for dead cells (red) indicated cytocompatibility. D) Alamar Blue Assay for metabolic activity indicated that 0% gels showed an increase in activity over 14 days, while 50% gels plateaued from day 7 to 14. E) Mammalian β-Galactosidase Assay for senescence revealed no change for any timepoints tested over 14 days. N=3-4, Error bars = SEM.TCP = Tissue Culture Plate, T-= without TGFβ, T+ = 5 ng/mL of TGFβ. Two-way ANOVA, Tukey’s: ns = not significant p > 0.05, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.

    Article Snippet: Normal human lung fibroblasts (NHLFs, PCS-201-013, ATCC, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium, high glucose (DMEM, ThermoFisher) supplemented with 10% Gibco Fetal Bovine Serum, Premium (FBS, ThermoFisher), 1% Gibco non-essential amino acids (ThermoFisher), and 1% Gibco antibiotic–antimycotic (ThermoFisher).

    Techniques: Modification, Imaging, Incubation, Alamar Blue Assay, Activity Assay

    Top row: NHLFs on 0% SF-TA hydrogels at Day 2, Day 7, and Day 14, exhibiting a proliferative, inflammatory phenotype consistent with early fibroblast activation, characterized by secretion of IL-6, IL-8, and MCP-1 (dots) and progressive collagen deposition (fibers). Bottom row: NHLFs on 50% SF-TA hydrogels at Day 2, Day 7, and Day 14, exhibiting metabolic plateau and lower inflammatory cytokine secretion, gradual collagen secretion, with TGFβ-dependent myofibroblast activation. Together, these two substrate conditions recapitulate distinct stages of the fibrotic disease continuum.

    Journal: bioRxiv

    Article Title: Progressive matrix stiffening of tyramine-modified silk fibroin hydrogels governs stage-specific pulmonary fibroblast activation

    doi: 10.64898/2026.06.01.729382

    Figure Lengend Snippet: Top row: NHLFs on 0% SF-TA hydrogels at Day 2, Day 7, and Day 14, exhibiting a proliferative, inflammatory phenotype consistent with early fibroblast activation, characterized by secretion of IL-6, IL-8, and MCP-1 (dots) and progressive collagen deposition (fibers). Bottom row: NHLFs on 50% SF-TA hydrogels at Day 2, Day 7, and Day 14, exhibiting metabolic plateau and lower inflammatory cytokine secretion, gradual collagen secretion, with TGFβ-dependent myofibroblast activation. Together, these two substrate conditions recapitulate distinct stages of the fibrotic disease continuum.

    Article Snippet: Normal human lung fibroblasts (NHLFs, PCS-201-013, ATCC, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium, high glucose (DMEM, ThermoFisher) supplemented with 10% Gibco Fetal Bovine Serum, Premium (FBS, ThermoFisher), 1% Gibco non-essential amino acids (ThermoFisher), and 1% Gibco antibiotic–antimycotic (ThermoFisher).

    Techniques: Activation Assay