mouse  (Proteintech)

Journal: Scientific Reports

Article Title: Endoplasmic reticulum stress sensor protein PERK in hepatic stellate cells promotes the progression of hepatocellular carcinoma via p38δ MAPK/IL-1β axis

doi: 10.1038/s41598-025-04150-w

Figure Lengend Snippet: PERK overexpression increases IL-1β expression in HSCs. PERK is overexpressed in LX-2 cells using a plasmid, and the expression of PERK, downstream molecules of PERK, and IL-1β is examined. ( a ) PERK mRNA expression is evaluated using RT-PCR (n = 4). ( b ) The expression of GFP-PERK, PERK, p-eIF2α, and eIF2α is determined using WB. Supplementary Fig. presents the original membranes of WB. ( c ) IL-1β mRNA expression is evaluated by RT-PCR (n = 4). ( d ) IL-1β protein expression is determined using WB. Supplementary Fig. presents the original membranes of WB. Data are shown as the mean ± SE. Statistical analysis is performed using Student’s t -test. ** p < 0.01. Abbreviations : PERK, Protein kinase R-like endoplasmic reticulum kinase; IL-1β, Interleukin-1 beta; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; WB, Western blotting; RT, Reverse transcription; siRNA, Small interfering RNA; HSCs, Hepatic stellate cells; SE, Standard error; GFP, Green fluorescent protein; p-eIF2α, Phosphorylated eukaryotic initiation factor 2-alpha.

Article Snippet: PERK (RG214993) and p38δ MAPK (RC200606) plasmids were obtained from Origene (Rockville, MD), and pCMV6 was used as the control plasmid.

Techniques: Over Expression, Expressing, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Reverse Transcription, Small Interfering RNA

Journal: Scientific Reports

Article Title: Endoplasmic reticulum stress sensor protein PERK in hepatic stellate cells promotes the progression of hepatocellular carcinoma via p38δ MAPK/IL-1β axis

doi: 10.1038/s41598-025-04150-w

Figure Lengend Snippet: PERK regulates p38δ MAPK expression in HSCs. RNA-sequencing analysis is performed using the RNAs collected from PA-treated LX-2 cells and PERK siRNA-transfected PA-treated LX-2 cells. DEGs between PA (n = 3) and PERK siRNA + PA (n = 3) are determined using CLC Genomics Workbench v24. The mRNAs with a fold change > 2 and FDR p -value < 0.05 between the two groups are selected as DEGs. ( a ) DEGs are visualized using the heatmap. ( b ) Volcano plots show that EIF2AK3 (PERK), IL1B (IL-1β), and MAPK13 (p38δ MAPK) are markedly downregulated in PERK siRNA + PA compared to PA. Furthermore, p38δ MAPK expression is examined in PERK-knockdown and PERK-overexpression LX-2 cells. ( c ) p38δ MAPK mRNA ( c ) and protein ( d ) expression are evaluated using RT-PCR (n = 4) and WB, respectively. Supplementary Fig. presents the original membranes of WB. p38δ MAPK mRNA ( e ) and protein ( f ) expression are evaluated using RT-PCR (n = 4) and WB, respectively. Supplementary Fig. shows the original membranes of WB. Data are presented as the mean ± SE. Statistical analysis is performed using Student’s t -test. ** p < 0.01. Abbreviations : PERK, protein kinase R-like endoplasmic reticulum kinase; IL-1β, interleukin-1 beta; MAPK, mitogen-activated protein kinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; WB, western blotting; RT, reverse transcription; siRNA, small interfering RNA; HSCs, hepatic stellate cells; SE, standard error; PA, palmitic acid; DEGs, differentially expressed genes; FDR, false discovery rate.

Article Snippet: PERK (RG214993) and p38δ MAPK (RC200606) plasmids were obtained from Origene (Rockville, MD), and pCMV6 was used as the control plasmid.

Techniques: Expressing, RNA Sequencing, Transfection, Knockdown, Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Reverse Transcription, Small Interfering RNA

Journal: Scientific Reports

Article Title: Endoplasmic reticulum stress sensor protein PERK in hepatic stellate cells promotes the progression of hepatocellular carcinoma via p38δ MAPK/IL-1β axis

doi: 10.1038/s41598-025-04150-w

Figure Lengend Snippet: PERK in HSCs promotes HCC progression via the p38δ MAPK/IL-1β axis. In the tumor microenvironment of MASH-related HCC, PA absorbed from the intestine induces ER stress in HSCs, activating PERK signaling. PERK subsequently promotes IL-1β secretion via the p38δ MAPK/IL-1β axis, enhancing the proliferation, migration, and invasion of HCC cells, thereby contributing to HCC progression. Abbreviations : MASH, metabolic dysfunction-associated steatohepatitis; HCC, hepatocellular carcinoma; HSCs, hepatic stellate cells; ER, endoplasmic reticulum; PERK, protein kinase R-like endoplasmic reticulum kinase; IL-1β, interleukin-1 beta; MAPK, mitogen-activated protein kinase; PA, palmitic acid.

Article Snippet: PERK (RG214993) and p38δ MAPK (RC200606) plasmids were obtained from Origene (Rockville, MD), and pCMV6 was used as the control plasmid.

Techniques: Migration

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Antibodies used for Western blots.

Article Snippet: Rabbit anti-human PERK , Protein Tech, China , 24390-1-AP , 1:1000.

Techniques: Western Blot