human pd  (ACROBiosystems)

Journal: Science Advances

Article Title: Beta cell–targeted PD-1 agonist inhibits cell-mediated autoimmunity in pancreas tissue slices

doi: 10.1126/sciadv.aec9029

Figure Lengend Snippet: ( A ) CF647-labeled ImmTAAI molecules were assessed for pHLA binding and compared to unlabeled molecules by SPR using BIAcore 8K. Binding to PPI or Tel cognate pHLA was carried out at 37°C. ( B ) Similarly, CF647-labeled ImmTAAI molecules were assessed for PD-1 binding and compared to unlabeled molecules. PD-1 binding was carried out at 25°C. ( C ) Schematic of the ECN90 beta cell line: Jurkat NFL Mel5 PD-1 reporter assay. ( D ) ECN90 or NCI-H1703 cells were pulsed with Melan-A–activating peptide, and titrations of unlabeled or labeled PPI ImmTAAI molecules were added.

Article Snippet: The T cell clone 4b was transduced with lentivirus containing PDCD1 (PD-1) (OriGene, #RC210364L1) as described ( ).

Techniques: Labeling, Binding Assay, Reporter Assay

Journal: Science Advances

Article Title: Beta cell–targeted PD-1 agonist inhibits cell-mediated autoimmunity in pancreas tissue slices

doi: 10.1126/sciadv.aec9029

Figure Lengend Snippet: The TCR targeting domain of ImmTAAI binds to PPI 15–24 peptide-HLA class I presented by beta cells. The PD-1 agonist effector domain inhibits T cell function and prevents beta cell killing. This mechanism is independent of the TCR specificity of the engaged cytotoxic T cell.

Article Snippet: The T cell clone 4b was transduced with lentivirus containing PDCD1 (PD-1) (OriGene, #RC210364L1) as described ( ).

Techniques: Cell Function Assay

Journal: bioRxiv

Article Title: Quantitative extrapolation from single-tags (QuEST) immunofluorescence microscopy to derive TCR signalosome stoichiometries in human primary T cells

doi: 10.64898/2026.03.28.715001

Figure Lengend Snippet: a – c , Workflow ( a ), representative TIRF images ( b ), and quantification results ( c ) of the number of signaling molecules per TCR following introduction of PD-1–PD-L1 inhibitory signaling in T cells. d , Stoichiometry of the TCR signalosome under PD-1–PD-L1 inhibitory signaling. e , Quantified sensitivity of signaling molecules within the TCR signalosome to PD-1–PD-L1 inhibitory signaling. f , Generation of T cells expressing low or high levels of PD-1. g , h , Representative TIRF images ( g ) and corresponding quantification results ( h ) of the number of signaling molecules per TCR in T cells expressing low or high levels of PD-1, with or without PD-1–PD-L1 inhibitory signaling. Data in f are presented as mean ± SD; data in c , d , and h are presented as mean ± SEM. In c , d , and h , ≥30 individual cells were analyzed per condition. Statistical significance was assessed using an unpaired two-tailed Student’s t -test (*** P ≤ 0.001). Data in c , d , and h are representative of two replicates using T cells from two blood donors. Data in f are representative of three replicates using T cells from three blood donors.

Article Snippet: Unlabelled primary antibodies : anti-human CD8α antibody (Cell Signaling Technology, Cat#85336), anti-human CD28 antibody (Cell Signaling Technology, Cat#38774S), anti-human CD45 antibody (Cell Signaling Technology, Cat#13917S), anti-human PD-1 antibody (Cell Signaling Technology, Cat#86163T), anti-human Lck antibody (Cell Signaling Technology, Cat#2787S), anti-human ZAP-70 antibody (Cell Signaling Technology, Cat#3165S), anti-human LAT antibody (Cell Signaling Technology, Cat#45533S), anti-human PLCγ1 antibody (Cell Signaling Technology, Cat#5690S), and anti-human phospho-ZAP-70 (Tyr319) antibody (Cell Signaling Technology, Cat#2701).

Techniques: Expressing, Two Tailed Test