Review



8x15k ‘human mirna microarray kit (v2)’  (Agilent technologies)


Bioz Verified Symbol Agilent technologies is a verified supplier
Bioz Manufacturer Symbol Agilent technologies manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Agilent technologies 8x15k ‘human mirna microarray kit (v2)’
    8x15k ‘Human Mirna Microarray Kit (V2)’, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mirna+microarray(v2/pmc04053776-290-14-25
    Average 90 stars, based on 1 article reviews
    8x15k ‘human mirna microarray kit (v2)’ - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Microarray:

    Article Title: Identification of a 4-microRNA Signature for Clear Cell Renal Cell Carcinoma Metastasis and Prognosis
    Article Snippet: .. Microarray testing of miRNA expression was performed at the COH Microarray Core using the Agilent human miRNA microarray V2 (Agilent Technologies, Inc., Santa Clara CA), which contains probes for 723 human miRNAs from Sanger miRBase 10.1, as described previously. ..

    Article Title: Deregulated MIR335 that targets MAPK1 is implicated in poor outcome of paediatric acute lymphoblastic leukaemia.
    Article Snippet: Junli Yan,* Nan Jiang,* Gaofeng Huang,* Jim L.-S. Tay, Baohong Lin, Chonglei Bi, Grace S. Koh, Zhenhua Li, Joy Tan, Tae-Hoon Chung, Yi Lu, Hany Ariffin, Shirley K. Y. Kham, Allen E. J. Yeoh and Wee-Joo Chng Cancer Science Institute of Singapore, National University of Singapore, Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Department of Haematology-Oncology, National University Cancer Institute of Singapore, National University Health System, Singapore City, Singapore, Department of Paediatrics, University Malaya Medical Centre, Kuala Lumpur, Malaysia and Viva-University Children’s Cancer Centre, University Children’s Medical Institute and National University Cancer Institute of Singapore, National University Health System, Singapore City, Singapore

    Article Title: MiR-10a and HOXB4 are overexpressed in atypical myeloproliferative neoplasms
    Article Snippet: Cyanine-3 (Cy3) labeled miRNA was prepared from 0.2 μg RNA using the miRNA complete labeling kit version 2.2 (Agilent Technologies). .. For each sample, the labeled miRNAs were hybridized overnight at 55 °C onto Human miRNA Microarray V2 (Agilent Technologies). ..

    Article Title: Impact of Host Genes and Strand Selection on miRNA and miRNA* Expression
    Article Snippet: .. The miRNA expression was profiled on the Agilent Human miRNA Microarray V2. ..

    Article Title: A tyrosine kinase-STAT5-miR21-PDCD4 regulatory axis in chronic and acute myeloid leukemia cells
    Article Snippet: Cyanine-3 (Cy3) labeled miRNAs were prepared from 0.2 μg RNA using the miRNA complete labeling kit version 2.2 (Agilent Technologies). .. Labeled miRNAs were hybridized overnight at 55°C onto Human miRNA Microarray V2 (Agilent Technologies) slides. .. The slides were then washed as recommended by the manufacturer, and scanned on an Agilent G2565CA scanner, at 5 μm resolution and using the 20-bit scan mode.

    Article Title: Identification of a miRNA signature in neutrophils after traumatic injury.
    Article Snippet: Traumatic injury is the cause of significant mortality and morbidity.. The molecular mechanisms underlying traumatic injury logically involve changes in gene expression that may be regulated through microRNAs (miRNAs).. However, the association between miRNA deregulation and traumatic injury is largely unknown.

    Article Title: Association between gene and miRNA expression profiles and stereotyped subset #4 B-cell receptor in chronic lymphocytic leukemia.
    Article Snippet: In this study we investigated specific biological and clinical features associated with chronic lymphocytic leukemia (CLL) patients carrying stereotyped BCR subset #4 (IGHV4-34) among a prospective cohort of 462 CLL/MBL patients in early stage (Binet A).. All subset #4 patients (n=16) were characterized by the IGHV mutated gene configuration, and absence of unfavorable cytogenetic lesions, NOTCH1 or SF3B1 mutations.. Gene and miRNA expression profiling evidenced that the leukemic cells of subset #4 L eu k L ym ph om a D ow nl oa de d fr om in fo rm ah ea lth ca re .c om b y K ai na n U ni ve rs ity o n 04 /1 2/ 15 Fo r pe rs on al u se o nl y.

    Article Title: The hsa-miR-302 cluster controls ectodermal differentiation of human pluripotent stem cell via repression of DAZAP2 .
    Article Snippet: .. Global miRNA expression profiles were also analyzed using an Agilent Human miRNA microarray v2 (Agilent, Santa Clara, CA, USA). .. Global mRNA expression profiles were analyzed using a Human Gene Expression 4 44K Microarray (Agilent).

    Expressing:

    Article Title: Identification of a 4-microRNA Signature for Clear Cell Renal Cell Carcinoma Metastasis and Prognosis
    Article Snippet: .. Microarray testing of miRNA expression was performed at the COH Microarray Core using the Agilent human miRNA microarray V2 (Agilent Technologies, Inc., Santa Clara CA), which contains probes for 723 human miRNAs from Sanger miRBase 10.1, as described previously. ..

    Article Title: Deregulated MIR335 that targets MAPK1 is implicated in poor outcome of paediatric acute lymphoblastic leukaemia.
    Article Snippet: Junli Yan,* Nan Jiang,* Gaofeng Huang,* Jim L.-S. Tay, Baohong Lin, Chonglei Bi, Grace S. Koh, Zhenhua Li, Joy Tan, Tae-Hoon Chung, Yi Lu, Hany Ariffin, Shirley K. Y. Kham, Allen E. J. Yeoh and Wee-Joo Chng Cancer Science Institute of Singapore, National University of Singapore, Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Department of Haematology-Oncology, National University Cancer Institute of Singapore, National University Health System, Singapore City, Singapore, Department of Paediatrics, University Malaya Medical Centre, Kuala Lumpur, Malaysia and Viva-University Children’s Cancer Centre, University Children’s Medical Institute and National University Cancer Institute of Singapore, National University Health System, Singapore City, Singapore

    Article Title: Impact of Host Genes and Strand Selection on miRNA and miRNA* Expression
    Article Snippet: .. The miRNA expression was profiled on the Agilent Human miRNA Microarray V2. ..

    Article Title: The hsa-miR-302 cluster controls ectodermal differentiation of human pluripotent stem cell via repression of DAZAP2 .
    Article Snippet: .. Global miRNA expression profiles were also analyzed using an Agilent Human miRNA microarray v2 (Agilent, Santa Clara, CA, USA). .. Global mRNA expression profiles were analyzed using a Human Gene Expression 4 44K Microarray (Agilent).

    Labeling:

    Article Title: MiR-10a and HOXB4 are overexpressed in atypical myeloproliferative neoplasms
    Article Snippet: Cyanine-3 (Cy3) labeled miRNA was prepared from 0.2 μg RNA using the miRNA complete labeling kit version 2.2 (Agilent Technologies). .. For each sample, the labeled miRNAs were hybridized overnight at 55 °C onto Human miRNA Microarray V2 (Agilent Technologies). ..

    Article Title: A tyrosine kinase-STAT5-miR21-PDCD4 regulatory axis in chronic and acute myeloid leukemia cells
    Article Snippet: Cyanine-3 (Cy3) labeled miRNAs were prepared from 0.2 μg RNA using the miRNA complete labeling kit version 2.2 (Agilent Technologies). .. Labeled miRNAs were hybridized overnight at 55°C onto Human miRNA Microarray V2 (Agilent Technologies) slides. .. The slides were then washed as recommended by the manufacturer, and scanned on an Agilent G2565CA scanner, at 5 μm resolution and using the 20-bit scan mode.

    Isolation:

    Article Title: Identification of a miRNA signature in neutrophils after traumatic injury.
    Article Snippet: Traumatic injury is the cause of significant mortality and morbidity.. The molecular mechanisms underlying traumatic injury logically involve changes in gene expression that may be regulated through microRNAs (miRNAs).. However, the association between miRNA deregulation and traumatic injury is largely unknown.

    Synthesized:

    Article Title: Association between gene and miRNA expression profiles and stereotyped subset #4 B-cell receptor in chronic lymphocytic leukemia.
    Article Snippet: In this study we investigated specific biological and clinical features associated with chronic lymphocytic leukemia (CLL) patients carrying stereotyped BCR subset #4 (IGHV4-34) among a prospective cohort of 462 CLL/MBL patients in early stage (Binet A).. All subset #4 patients (n=16) were characterized by the IGHV mutated gene configuration, and absence of unfavorable cytogenetic lesions, NOTCH1 or SF3B1 mutations.. Gene and miRNA expression profiling evidenced that the leukemic cells of subset #4 L eu k L ym ph om a D ow nl oa de d fr om in fo rm ah ea lth ca re .c om b y K ai na n U ni ve rs ity o n 04 /1 2/ 15 Fo r pe rs on al u se o nl y.

    In Situ:

    Article Title: Association between gene and miRNA expression profiles and stereotyped subset #4 B-cell receptor in chronic lymphocytic leukemia.
    Article Snippet: In this study we investigated specific biological and clinical features associated with chronic lymphocytic leukemia (CLL) patients carrying stereotyped BCR subset #4 (IGHV4-34) among a prospective cohort of 462 CLL/MBL patients in early stage (Binet A).. All subset #4 patients (n=16) were characterized by the IGHV mutated gene configuration, and absence of unfavorable cytogenetic lesions, NOTCH1 or SF3B1 mutations.. Gene and miRNA expression profiling evidenced that the leukemic cells of subset #4 L eu k L ym ph om a D ow nl oa de d fr om in fo rm ah ea lth ca re .c om b y K ai na n U ni ve rs ity o n 04 /1 2/ 15 Fo r pe rs on al u se o nl y.



    Similar Products

    90
    Phalanx Biotech mirna microarrays human mirna onearray® v2
    Mirna Microarrays Human Mirna Onearray® V2, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mirna+microarray(v2/human+onearray+mirna+v2/pm39930542-125-26-34
    Average 90 stars, based on 1 article reviews
    mirna microarrays human mirna onearray® v2 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Phalanx Biotech mirna microarrays human mirna onearray v2
    miR-4259 targets the LDHA 3’UTR and inhibits LDHA-mediated gemcitabine resistance in PDAC. ( A ) The miR-4259 expression (left) and LDHA -3’UTR luciferase activity (right) in PANC-1 and PANC-1/GEM cells was measured by RT-qPCR and a luciferase reporter assay, respectively. The RT-qPCR data were normalized to the level of U47 RNA in each individual sample. ( B ) A schematic diagram representing the predicted miR-4259-binding sequences or the mutated versions of the <t>miRNA</t> (left). The luciferase reporter activity (right) of the LDHA -3’UTR wild-type (+ 1 ~ + 937) and LDHA -3’UTR mutant reporters (mutant sites: 498, 498/518 and 498/518/818) were measured by a dual-luciferase reporter assay in HEK-293T cells transfected with miR-4259 and a reporter at different ratios. ( C ) The luciferase reporter activity of the LDHA -3’UTR wild-type and LDHA -3’UTR mutant reporters (triple-mutant sites, 498/518/818) in PANC-1/GEM and SUIT-2 cells and their expression of miR-4259. ( D ) The LDHA and miR-4259 expression (left) of PANC-1/GEM cells transfected with the indicated plasmids were analyzed by Western blotting and RT-qPCR, respectively. The cell viability (right) of these transfectants in the presence of gemcitabine treatment was measured by the MTT assay. ( E ) The LDHA and miR-4259 expression (left) of PANC-1 cells were analyzed by Western blotting and RT-qPCR, respectively. The cell viability ( right ) of these transfectants in the presence of gemcitabine treatment was measured by the MTT assay. The results are presented as the means ± s.e.m. of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s. not significant (two-tailed Student’s t -test)
    Mirna Microarrays Human Mirna Onearray V2, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mirna+microarray(v2/human+onearray+mirna+v2/pmc11809001-115-16-25
    Average 90 stars, based on 1 article reviews
    mirna microarrays human mirna onearray v2 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies 8x15k ‘human mirna microarray kit (v2)’
    miR-4259 targets the LDHA 3’UTR and inhibits LDHA-mediated gemcitabine resistance in PDAC. ( A ) The miR-4259 expression (left) and LDHA -3’UTR luciferase activity (right) in PANC-1 and PANC-1/GEM cells was measured by RT-qPCR and a luciferase reporter assay, respectively. The RT-qPCR data were normalized to the level of U47 RNA in each individual sample. ( B ) A schematic diagram representing the predicted miR-4259-binding sequences or the mutated versions of the <t>miRNA</t> (left). The luciferase reporter activity (right) of the LDHA -3’UTR wild-type (+ 1 ~ + 937) and LDHA -3’UTR mutant reporters (mutant sites: 498, 498/518 and 498/518/818) were measured by a dual-luciferase reporter assay in HEK-293T cells transfected with miR-4259 and a reporter at different ratios. ( C ) The luciferase reporter activity of the LDHA -3’UTR wild-type and LDHA -3’UTR mutant reporters (triple-mutant sites, 498/518/818) in PANC-1/GEM and SUIT-2 cells and their expression of miR-4259. ( D ) The LDHA and miR-4259 expression (left) of PANC-1/GEM cells transfected with the indicated plasmids were analyzed by Western blotting and RT-qPCR, respectively. The cell viability (right) of these transfectants in the presence of gemcitabine treatment was measured by the MTT assay. ( E ) The LDHA and miR-4259 expression (left) of PANC-1 cells were analyzed by Western blotting and RT-qPCR, respectively. The cell viability ( right ) of these transfectants in the presence of gemcitabine treatment was measured by the MTT assay. The results are presented as the means ± s.e.m. of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s. not significant (two-tailed Student’s t -test)
    8x15k ‘Human Mirna Microarray Kit (V2)’, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mirna+microarray(v2/pmc04053776-290-14-25
    Average 90 stars, based on 1 article reviews
    8x15k ‘human mirna microarray kit (v2)’ - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies human mirna microarray (v2
    The differences between <t> microarray </t> platforms used in the project ( source: Affymetrix and Agilent data sheets )
    Human Mirna Microarray (V2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mirna+microarray(v2/pmc03562514-63-25-24
    Average 90 stars, based on 1 article reviews
    human mirna microarray (v2 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    93
    Agilent technologies human mirna microarray kit (v2
    Differentially expressed miRNAs in human PBLs incubated in MMG. (a) The expression level of each <t>miRNA,</t> indicated as fold change, is the mean of the expression values obtained from the transformed log2 ratio (MMG/1 g). (b) Dendrogram of miRNAs differentially expressed in MMG. The range of expression value is from −3.7 (green, downregulation) to 3.07 (red, upregulation). Grey boxes correspond to not available (N/A) fluorescent signal from the <t>microarray</t> platform.
    Human Mirna Microarray Kit (V2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mirna+microarray(v2/R-Phycoerythrin+Conjugation+Kit/pmc04090438-65-20-25
    Average 93 stars, based on 1 article reviews
    human mirna microarray kit (v2 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    90
    Agilent technologies human mirna microarray kit v2
    Gene expression analysis of the different GCT subgroups . ( A ) A multidimensional scaling (MDS) plot containing the differentially expressed genes (690 probe sets, q < 0.001). Each spot represents a single array. ( B ) A comparison of the transcriptome traits between ESCs and NGMGCTs by principal component analysis (PCA). ( C ) Relationships between ESCs, germinomas and NGMGCTs. Average linkage Euclidean distances between the tissues and ESC were calculated using genes distinguishing the filtrated 690-probe set. The confidence limits shown represent the standard error. ( D ) A heat map shows genes enriched in the ESCs and in the different prognostic groups ( q < 0.001). ( E-F ) Real-time PCR validation of the <t>microarray</t> data. Mean expression levels of the examined genes were compared to that of the GAPDH control. Each bar represents a different individual ( F ). The genes' array hybridization signals are also shown ( E ).
    Human Mirna Microarray Kit V2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mirna+microarray(v2/pmc02837036-480-2-7
    Average 90 stars, based on 1 article reviews
    human mirna microarray kit v2 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies human mirna microarray v2
    Gene expression analysis of the different GCT subgroups . ( A ) A multidimensional scaling (MDS) plot containing the differentially expressed genes (690 probe sets, q < 0.001). Each spot represents a single array. ( B ) A comparison of the transcriptome traits between ESCs and NGMGCTs by principal component analysis (PCA). ( C ) Relationships between ESCs, germinomas and NGMGCTs. Average linkage Euclidean distances between the tissues and ESC were calculated using genes distinguishing the filtrated 690-probe set. The confidence limits shown represent the standard error. ( D ) A heat map shows genes enriched in the ESCs and in the different prognostic groups ( q < 0.001). ( E-F ) Real-time PCR validation of the <t>microarray</t> data. Mean expression levels of the examined genes were compared to that of the GAPDH control. Each bar represents a different individual ( F ). The genes' array hybridization signals are also shown ( E ).
    Human Mirna Microarray V2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+mirna+microarray(v2/pmc03166117-157-8-7
    Average 90 stars, based on 1 article reviews
    human mirna microarray v2 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    miR-4259 targets the LDHA 3’UTR and inhibits LDHA-mediated gemcitabine resistance in PDAC. ( A ) The miR-4259 expression (left) and LDHA -3’UTR luciferase activity (right) in PANC-1 and PANC-1/GEM cells was measured by RT-qPCR and a luciferase reporter assay, respectively. The RT-qPCR data were normalized to the level of U47 RNA in each individual sample. ( B ) A schematic diagram representing the predicted miR-4259-binding sequences or the mutated versions of the miRNA (left). The luciferase reporter activity (right) of the LDHA -3’UTR wild-type (+ 1 ~ + 937) and LDHA -3’UTR mutant reporters (mutant sites: 498, 498/518 and 498/518/818) were measured by a dual-luciferase reporter assay in HEK-293T cells transfected with miR-4259 and a reporter at different ratios. ( C ) The luciferase reporter activity of the LDHA -3’UTR wild-type and LDHA -3’UTR mutant reporters (triple-mutant sites, 498/518/818) in PANC-1/GEM and SUIT-2 cells and their expression of miR-4259. ( D ) The LDHA and miR-4259 expression (left) of PANC-1/GEM cells transfected with the indicated plasmids were analyzed by Western blotting and RT-qPCR, respectively. The cell viability (right) of these transfectants in the presence of gemcitabine treatment was measured by the MTT assay. ( E ) The LDHA and miR-4259 expression (left) of PANC-1 cells were analyzed by Western blotting and RT-qPCR, respectively. The cell viability ( right ) of these transfectants in the presence of gemcitabine treatment was measured by the MTT assay. The results are presented as the means ± s.e.m. of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s. not significant (two-tailed Student’s t -test)

    Journal: Cancer & Metabolism

    Article Title: FOXO3a/miR-4259-driven LDHA expression as a key mechanism of gemcitabine sensitivity in pancreatic ductal adenocarcinoma

    doi: 10.1186/s40170-025-00377-3

    Figure Lengend Snippet: miR-4259 targets the LDHA 3’UTR and inhibits LDHA-mediated gemcitabine resistance in PDAC. ( A ) The miR-4259 expression (left) and LDHA -3’UTR luciferase activity (right) in PANC-1 and PANC-1/GEM cells was measured by RT-qPCR and a luciferase reporter assay, respectively. The RT-qPCR data were normalized to the level of U47 RNA in each individual sample. ( B ) A schematic diagram representing the predicted miR-4259-binding sequences or the mutated versions of the miRNA (left). The luciferase reporter activity (right) of the LDHA -3’UTR wild-type (+ 1 ~ + 937) and LDHA -3’UTR mutant reporters (mutant sites: 498, 498/518 and 498/518/818) were measured by a dual-luciferase reporter assay in HEK-293T cells transfected with miR-4259 and a reporter at different ratios. ( C ) The luciferase reporter activity of the LDHA -3’UTR wild-type and LDHA -3’UTR mutant reporters (triple-mutant sites, 498/518/818) in PANC-1/GEM and SUIT-2 cells and their expression of miR-4259. ( D ) The LDHA and miR-4259 expression (left) of PANC-1/GEM cells transfected with the indicated plasmids were analyzed by Western blotting and RT-qPCR, respectively. The cell viability (right) of these transfectants in the presence of gemcitabine treatment was measured by the MTT assay. ( E ) The LDHA and miR-4259 expression (left) of PANC-1 cells were analyzed by Western blotting and RT-qPCR, respectively. The cell viability ( right ) of these transfectants in the presence of gemcitabine treatment was measured by the MTT assay. The results are presented as the means ± s.e.m. of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s. not significant (two-tailed Student’s t -test)

    Article Snippet: Five micrograms of total RNA obtained from PANC-1 and PANC-1/GEM cells were labeled and hybridized on miRNA microarrays (using the Human miRNA OneArray ® v2 (Phalanx Biotech Group, San Diego, CA, USA).

    Techniques: Expressing, Luciferase, Activity Assay, Quantitative RT-PCR, Reporter Assay, Binding Assay, Mutagenesis, Transfection, Western Blot, MTT Assay, Two Tailed Test

    The differences between  microarray  platforms used in the project ( source: Affymetrix and Agilent data sheets )

    Journal: BMC Genomics

    Article Title: MMpred: functional miRNA – mRNA interaction analyses by miRNA expression prediction

    doi: 10.1186/1471-2164-13-620

    Figure Lengend Snippet: The differences between microarray platforms used in the project ( source: Affymetrix and Agilent data sheets )

    Article Snippet: Such impediments are reflected in the relatively small number of paired miRNA-mRNA datasets available in public repositories - ( i.e. there are only nine Agilent Human miRNA Microarray (V2) datasets in GEO [ , ]; see Additional file ).

    Techniques: Microarray, Sequencing

    Differentially expressed miRNAs in human PBLs incubated in MMG. (a) The expression level of each miRNA, indicated as fold change, is the mean of the expression values obtained from the transformed log2 ratio (MMG/1 g). (b) Dendrogram of miRNAs differentially expressed in MMG. The range of expression value is from −3.7 (green, downregulation) to 3.07 (red, upregulation). Grey boxes correspond to not available (N/A) fluorescent signal from the microarray platform.

    Journal: BioMed Research International

    Article Title: Integration Analysis of MicroRNA and mRNA Expression Profiles in Human Peripheral Blood Lymphocytes Cultured in Modeled Microgravity

    doi: 10.1155/2014/296747

    Figure Lengend Snippet: Differentially expressed miRNAs in human PBLs incubated in MMG. (a) The expression level of each miRNA, indicated as fold change, is the mean of the expression values obtained from the transformed log2 ratio (MMG/1 g). (b) Dendrogram of miRNAs differentially expressed in MMG. The range of expression value is from −3.7 (green, downregulation) to 3.07 (red, upregulation). Grey boxes correspond to not available (N/A) fluorescent signal from the microarray platform.

    Article Snippet: MicroRNAs profiling was carried out in PBL samples incubated in MMG versus 1 g. Analyses were performed by using the “Human miRNA Microarray kit (V2)” (Agilent Technologies) that allows the detection of 723 known human (miRBase v.10.1) and 76 human viral miRNAs.

    Techniques: Incubation, Expressing, Transformation Assay, Microarray

    Microarray data validation by quantitative real-time PCR (qRT-PCR). Validation of microarray data by qRT-PCR in MMG-incubated versus 1 g incubated PBLs. The results are consistent with the cumulative microarray data of miRNAs (a) and mRNAs (b). Values (fold change, dark grey bars) are means ± S.E. of expression levels calculated as the log2 (MMG/1 g) on PBL samples from 4 to 6 different donors. The value “1” of control 1 g PBLs (light grey bars) is arbitrarily given when no change is observed (*** P < 0.001, ** P < 0.01, and * P < 0.05, t -test).

    Journal: BioMed Research International

    Article Title: Integration Analysis of MicroRNA and mRNA Expression Profiles in Human Peripheral Blood Lymphocytes Cultured in Modeled Microgravity

    doi: 10.1155/2014/296747

    Figure Lengend Snippet: Microarray data validation by quantitative real-time PCR (qRT-PCR). Validation of microarray data by qRT-PCR in MMG-incubated versus 1 g incubated PBLs. The results are consistent with the cumulative microarray data of miRNAs (a) and mRNAs (b). Values (fold change, dark grey bars) are means ± S.E. of expression levels calculated as the log2 (MMG/1 g) on PBL samples from 4 to 6 different donors. The value “1” of control 1 g PBLs (light grey bars) is arbitrarily given when no change is observed (*** P < 0.001, ** P < 0.01, and * P < 0.05, t -test).

    Article Snippet: MicroRNAs profiling was carried out in PBL samples incubated in MMG versus 1 g. Analyses were performed by using the “Human miRNA Microarray kit (V2)” (Agilent Technologies) that allows the detection of 723 known human (miRBase v.10.1) and 76 human viral miRNAs.

    Techniques: Microarray, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Incubation, Expressing

    Gene expression analysis of the different GCT subgroups . ( A ) A multidimensional scaling (MDS) plot containing the differentially expressed genes (690 probe sets, q < 0.001). Each spot represents a single array. ( B ) A comparison of the transcriptome traits between ESCs and NGMGCTs by principal component analysis (PCA). ( C ) Relationships between ESCs, germinomas and NGMGCTs. Average linkage Euclidean distances between the tissues and ESC were calculated using genes distinguishing the filtrated 690-probe set. The confidence limits shown represent the standard error. ( D ) A heat map shows genes enriched in the ESCs and in the different prognostic groups ( q < 0.001). ( E-F ) Real-time PCR validation of the microarray data. Mean expression levels of the examined genes were compared to that of the GAPDH control. Each bar represents a different individual ( F ). The genes' array hybridization signals are also shown ( E ).

    Journal: BMC Genomics

    Article Title: Pediatric primary central nervous system germ cell tumors of different prognosis groups show characteristic miRNome traits and chromosome copy number variations

    doi: 10.1186/1471-2164-11-132

    Figure Lengend Snippet: Gene expression analysis of the different GCT subgroups . ( A ) A multidimensional scaling (MDS) plot containing the differentially expressed genes (690 probe sets, q < 0.001). Each spot represents a single array. ( B ) A comparison of the transcriptome traits between ESCs and NGMGCTs by principal component analysis (PCA). ( C ) Relationships between ESCs, germinomas and NGMGCTs. Average linkage Euclidean distances between the tissues and ESC were calculated using genes distinguishing the filtrated 690-probe set. The confidence limits shown represent the standard error. ( D ) A heat map shows genes enriched in the ESCs and in the different prognostic groups ( q < 0.001). ( E-F ) Real-time PCR validation of the microarray data. Mean expression levels of the examined genes were compared to that of the GAPDH control. Each bar represents a different individual ( F ). The genes' array hybridization signals are also shown ( E ).

    Article Snippet: The Agilent Human miRNA Microarray Kit V2 (Agilent, Foster City, CA, USA) containing probes for 723 human microRNAs from the Sanger database v10.1 was used.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Microarray, Hybridization