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human lncrna expression microarray v3.0 (8 × 60 k)  (Arraystar inc)

 
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    Structured Review

    Arraystar inc human lncrna expression microarray v3.0 (8 × 60 k)
    Schematic diagram of the signalling pathways regulated by NIPA1-SO . Our study has uncovered an athero-protective role of the <t>lncRNA</t> NIPA1-SO , which, by interacting with a single transcription factor, is capable of inhibiting monocyte adhesion and foam cell formation, two fundamental processes in atherosclerosis. The transcription factor FUBP1 negatively regulates NIPA1 expression; this inhibitory effect is increased by the interaction of NIPA1-SO with FUBP1, resulting in lower transcription of NIPA1. A reduction in NIPA1 protein results in increased BMPR2 protein due to a reduction in NIPA1-mediated BMPR2 endocytosis and degradation, leading to higher levels of Smad1/5/8 phosphorylation (pSmad1/5/8), which, through complexation with Smad4, inhibit transcription of the adhesion molecules VCAM1 and ICAM1, reducing monocyte adhesion to endothelial cells. Further, the pSmad1/5/8:Smad4 complex promotes ABCA1 and ABCG1 transcription, both of which promote cholesterol efflux via high-density lipoprotein (HDL) particles, thereby inhibiting foam cell formation.
    Human Lncrna Expression Microarray V3.0 (8 × 60 K), supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lncrna+expression+microarray+v3%2E0/human+lncrna+microarray+v3+0/pmc10703723-234-5-14
    Average 90 stars, based on 1 article reviews
    human lncrna expression microarray v3.0 (8 × 60 k) - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1"

    Article Title: LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1

    Journal: Journal of Advanced Research

    doi: 10.1016/j.jare.2023.01.017

    Schematic diagram of the signalling pathways regulated by NIPA1-SO . Our study has uncovered an athero-protective role of the lncRNA NIPA1-SO , which, by interacting with a single transcription factor, is capable of inhibiting monocyte adhesion and foam cell formation, two fundamental processes in atherosclerosis. The transcription factor FUBP1 negatively regulates NIPA1 expression; this inhibitory effect is increased by the interaction of NIPA1-SO with FUBP1, resulting in lower transcription of NIPA1. A reduction in NIPA1 protein results in increased BMPR2 protein due to a reduction in NIPA1-mediated BMPR2 endocytosis and degradation, leading to higher levels of Smad1/5/8 phosphorylation (pSmad1/5/8), which, through complexation with Smad4, inhibit transcription of the adhesion molecules VCAM1 and ICAM1, reducing monocyte adhesion to endothelial cells. Further, the pSmad1/5/8:Smad4 complex promotes ABCA1 and ABCG1 transcription, both of which promote cholesterol efflux via high-density lipoprotein (HDL) particles, thereby inhibiting foam cell formation.
    Figure Legend Snippet: Schematic diagram of the signalling pathways regulated by NIPA1-SO . Our study has uncovered an athero-protective role of the lncRNA NIPA1-SO , which, by interacting with a single transcription factor, is capable of inhibiting monocyte adhesion and foam cell formation, two fundamental processes in atherosclerosis. The transcription factor FUBP1 negatively regulates NIPA1 expression; this inhibitory effect is increased by the interaction of NIPA1-SO with FUBP1, resulting in lower transcription of NIPA1. A reduction in NIPA1 protein results in increased BMPR2 protein due to a reduction in NIPA1-mediated BMPR2 endocytosis and degradation, leading to higher levels of Smad1/5/8 phosphorylation (pSmad1/5/8), which, through complexation with Smad4, inhibit transcription of the adhesion molecules VCAM1 and ICAM1, reducing monocyte adhesion to endothelial cells. Further, the pSmad1/5/8:Smad4 complex promotes ABCA1 and ABCG1 transcription, both of which promote cholesterol efflux via high-density lipoprotein (HDL) particles, thereby inhibiting foam cell formation.

    Techniques Used: Expressing, Phospho-proteomics

    Related Articles

    Cell Culture:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

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    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
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    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    Expressing:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    Microarray:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    Labeling:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    Control:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    Whisker Assay:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    Quantitative Proteomics:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    Quantitative RT-PCR:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    Two Tailed Test:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    MANN-WHITNEY:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    Concentration Assay:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    Isolation:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    Gene Expression:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).

    RNA In Situ Hybridization:

    Article Title: Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA
    Article Snippet: To catalog expression changes in neighboring mRNA−lncRNA pairs during the EC response to inflammatory stimuli, human umbilical vein ECs (HUVECs) were treated with the proinflammatory cytokine, IL-1β, for either 4 or 24 h. RNA was isolated and converted to complementary DNA (cDNA), which was then hybridized to the Arraystar Human lncRNA Expression Microarray V3.0 (containing unique probes for 23,089 mRNAs and 21,339 lncRNAs; Dataset S1 ) (Gene Expression Omnibus [GEO] ID code GSE127990).

    Article Title: Transcriptomics of angiotensin II-induced long noncoding and coding RNAs in endothelial cells
    Article Snippet: Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and treated with Ang II (10 6mol/l) for 24 h. The cells were then profiled for the expression of lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.
    Article Snippet: Downregulation of lncRNA GCAT1 in GCs is clinically related with POI Using the Arraystar human lncRNA expression microarray v3.0, we previously17 explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697).

    Article Title: The Long Noncoding RNA RP11-728F11.4 Promotes Atherosclerosis
    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8×60K, Arraystar) and scanned using Agilent Scanner G2505C.

    Article Title: lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1
    Article Snippet: Using the Arraystar human lncRNA expression microarray v3.0, we previously explored the global expression profile of lncRNAs in GCs of bPOI patients (n = 10) and controls (n = 10) (GenBank: GSE135697 ).

    Article Title: Pravastatin‐induced changes in expression of long non‐coding and coding RNAs in endothelial cells
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) treated with pravastatin (10 μM) for 24 hr were profiled for lncRNAs and mRNAs using the Arraystar Human lncRNA Expression Microarray V3.0.

    Article Title: LncRNA serves as a biomarker and therapeutic target
    Article Snippet: Total RNA was subjected to human lncRNA expression microarray (V3.0) analyses (ArrayStar, Rockville, Mass.).



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    Image Search Results


    Schematic diagram of the signalling pathways regulated by NIPA1-SO . Our study has uncovered an athero-protective role of the lncRNA NIPA1-SO , which, by interacting with a single transcription factor, is capable of inhibiting monocyte adhesion and foam cell formation, two fundamental processes in atherosclerosis. The transcription factor FUBP1 negatively regulates NIPA1 expression; this inhibitory effect is increased by the interaction of NIPA1-SO with FUBP1, resulting in lower transcription of NIPA1. A reduction in NIPA1 protein results in increased BMPR2 protein due to a reduction in NIPA1-mediated BMPR2 endocytosis and degradation, leading to higher levels of Smad1/5/8 phosphorylation (pSmad1/5/8), which, through complexation with Smad4, inhibit transcription of the adhesion molecules VCAM1 and ICAM1, reducing monocyte adhesion to endothelial cells. Further, the pSmad1/5/8:Smad4 complex promotes ABCA1 and ABCG1 transcription, both of which promote cholesterol efflux via high-density lipoprotein (HDL) particles, thereby inhibiting foam cell formation.

    Journal: Journal of Advanced Research

    Article Title: LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1

    doi: 10.1016/j.jare.2023.01.017

    Figure Lengend Snippet: Schematic diagram of the signalling pathways regulated by NIPA1-SO . Our study has uncovered an athero-protective role of the lncRNA NIPA1-SO , which, by interacting with a single transcription factor, is capable of inhibiting monocyte adhesion and foam cell formation, two fundamental processes in atherosclerosis. The transcription factor FUBP1 negatively regulates NIPA1 expression; this inhibitory effect is increased by the interaction of NIPA1-SO with FUBP1, resulting in lower transcription of NIPA1. A reduction in NIPA1 protein results in increased BMPR2 protein due to a reduction in NIPA1-mediated BMPR2 endocytosis and degradation, leading to higher levels of Smad1/5/8 phosphorylation (pSmad1/5/8), which, through complexation with Smad4, inhibit transcription of the adhesion molecules VCAM1 and ICAM1, reducing monocyte adhesion to endothelial cells. Further, the pSmad1/5/8:Smad4 complex promotes ABCA1 and ABCG1 transcription, both of which promote cholesterol efflux via high-density lipoprotein (HDL) particles, thereby inhibiting foam cell formation.

    Article Snippet: Labeled cRNAs were hybridized with Human LncRNA Expression Microarray v3.0 (8 × 60 K, Arraystar) and scanned using Agilent Scanner G2505C.

    Techniques: Expressing, Phospho-proteomics

    TaqMan qRT-PCR validation of lncRNA microarray analysis. Fold-change in comparison of ACCversusNAC, *P < 0.05.

    Journal: Surgery

    Article Title: Adrenocortical tumors have a distinct, long, non-coding RNA expression profile and LINC00271 is downregulated in malignancy *

    doi: 10.1016/j.surg.2019.04.067

    Figure Lengend Snippet: TaqMan qRT-PCR validation of lncRNA microarray analysis. Fold-change in comparison of ACCversusNAC, *P < 0.05.

    Article Snippet: LncRNA expression profile using ArrayStar Human LncRNA/mRNA Expression Microarray V3.0 was analyzed in samples from 11 ACA, 9 ACC, and 5 NAC.

    Techniques: Quantitative RT-PCR, Biomarker Discovery, Microarray, Comparison