ldl  (Lee Biosolutions)

Journal: medRxiv

Article Title: Gene-gene interactions protect against Familial Hypercholesterolemia: effect of lost- of-function PCSK9 variants

doi: 10.64898/2026.03.26.26348145

Figure Lengend Snippet: Pedigree of the family showing segregation of LDLR and PCSK9 variants. Squares represent males, circles are females and diamond symbols are used to represent family members without specifying their sex. Individuals fulfilling the clinical diagnosis criteria for familial hypercholesterolemia (FH) are indicated by half-filled symbols. The first row below each symbol correspond to the individual ID following by the genotype at LDLR, PCSK9 and APOB ..

Article Snippet: Human LDLR cDNA constructs were generated using a GFP-tagged plasmid vector (C-GFPSpark®, NM_000527; Sino Biological, Beijing, China).

Techniques: Biomarker Discovery

Journal: medRxiv

Article Title: Gene-gene interactions protect against Familial Hypercholesterolemia: effect of lost- of-function PCSK9 variants

doi: 10.64898/2026.03.26.26348145

Figure Lengend Snippet: Functional characterization of LDLR c.2479G>A ; (A) Flow cytometry histogram overlay showing DiI-LDL binding; (B) Quantification of LDL uptake relative to WT; (C) LDLR expression. The upper line represents the benignity cutoff (90%) established by the ACMG/ClinGen guidelines for functional studies. * <0.05; **** <0.0001.

Article Snippet: Human LDLR cDNA constructs were generated using a GFP-tagged plasmid vector (C-GFPSpark®, NM_000527; Sino Biological, Beijing, China).

Techniques: Functional Assay, Flow Cytometry, Binding Assay, Expressing

Journal: Journal of Biomedical Research

Article Title: Remote neuromuscular electrical stimulation upregulates MDK to enhance macrophage efferocytosis via LRP1 in wound healing

doi: 10.7555/JBR.38.20240375

Figure Lengend Snippet: MDK upregulated LRP1 to improve efferocytosis through the PDI-ADAM17 axis. A: Bone marrow-derived macrophages (BMDMs) were treated with LPS (1 μg/mL) for 12 h. Apoptotic neutrophils (green) were added to BMDMs, with or without rMDK (200 ng/mL) for 12 h. LRPAP (50 μmol/L) was applied 0.5 h before rMDK. Confocal microscopy was used to quantify the phagocytosis index. Scale bars, 50 μm. B–D: LRP1 protein levels in RAW264.7 cells. Cells were treated with LPS (1 μg/mL) for 0, 3, 6, 12, 24 h (B), with LPS (1 μg/mL) for 24 h and rMDK for 3, 6, 12, 24 h (C), or with LPS (1 μg/mL) for 24 h with or without rMDK (200 ng/mL) for 12 h (D). E: LRP1 protein levels in the wound model. F and G: Lrp1 mRNA (F) and p-ADAM17 protein (G) levels in RAW264.7 cells treated as in (D). H: PDI protein levels in the supernatant of RAW264.7 cell cultures. I and J: p-ADAM17 (I) and PDI (J) protein levels in the wound. K: PDI protein levels in the plasma. Protein and mRNA levels were analyzed by Western blotting and reverse transcription-quantitative PCR, respectively. All data are presented as mean ± standard error of the mean. * P < 0.05, ** P < 0.01, and *** P < 0.001 by one-way ANOVA followed by Tukey's multiple comparisons test (A–K). Abbreviations: LPS, lipopolysaccharide; LRP1, LDL receptor-related protein 1; LRPAP, LDL receptor-related protein-associated protein; ns, not significant; p-ADAM17, phospho-a disintegrin and metalloproteinase domain-containing protein 17; PDI, protein disulfide isomerase; rMDK, recombinant midkine; rNMES, remote neuromuscular electrical stimulation.

Article Snippet: LDL receptor-related protein-associated protein (LRPAP; Cat. #HY-P76478A, MedChemExpress, Monmouth Junction, NJ, USA) was used as an LRP1 inhibitor.

Techniques: Derivative Assay, Confocal Microscopy, Clinical Proteomics, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Recombinant

Journal: Journal of Biomedical Research

Article Title: Remote neuromuscular electrical stimulation upregulates MDK to enhance macrophage efferocytosis via LRP1 in wound healing

doi: 10.7555/JBR.38.20240375

Figure Lengend Snippet: A schematic diagram showing the mechanism of rNMES in wound healing. rNMES induces MDK production in skeletal muscles through the AMPK-ERK axis. The secreted MDK then reaches the wound via blood circulation, enhances LRP1-mediated efferocytosis, and accelerates wound healing. Abbreviations: MDK, midkine; LRP1, low-density lipoprotein receptor-related protein 1; sLRP1, soluble LRP1; p-ADAM17, phospho-a disintegrin and metalloproteinase domain-containing protein 17; PDI, protein disulfide isomerase; p-AMPK, phospho-AMP-activated protein kinase; p-ERK, phospho-extracellular signal-regulated kinase; PMN, polymorphonuclear neutrophil; RBC, red blood cell; rNMES, remote neuromuscular electrical stimulation; NETs, neutrophil extracellular traps; ACs, apoptotic cells; E. coli , Escherichia coli .

Article Snippet: LDL receptor-related protein-associated protein (LRPAP; Cat. #HY-P76478A, MedChemExpress, Monmouth Junction, NJ, USA) was used as an LRP1 inhibitor.

Techniques: Muscles

Journal: International Journal of Molecular Sciences

Article Title: Twenty-Four-Month rhGH Intervention: Insights into Redox Regulation, Vascular Biomarkers, and Body Composition in Adult GHD Patients

doi: 10.3390/ijms27031451

Figure Lengend Snippet: Longitudinal changes in ox-LDL, Trx, E-selectin, P-selectin, ICAM-1, and VCAM-1 during 24-month rhGH therapy at baseline, 12 months, and 24 months. Statistical significance is indicated by asterisks: * p < 0.05 vs. baseline; *** p < 0.001 vs. baseline; **** p < 0.00001 vs. baseline.

Article Snippet: OS and endothelial markers were quantified using commercially available enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturers’ instructions: ox-LDL (Novus Biologicals, Bio-Techne, NBP3-18794; Minneapolis, MN, USA), Trx (XpressBio, XPEH0291; Frederick, MD, USA), OGG1 (Cloud-Clone Corp., SEC704Hu; Wuhan, China), VCAM-1 (Cloud-Clone Corp., SEA547Hu; Wuhan, China), ICAM-1 (Cloud-Clone Corp., SEA548Ca; Wuhan, China), and E-selectin (Cloud-Clone Corp., SEA029Hu; Wuhan, China).

Techniques: