isolated human subcutaneous preadipocytes  (ZenBio)

Journal: Translational Oncology

Article Title: Adipocytes Isolated from Visceral and Subcutaneous Depots of Donors Differing in BMI Crosstalk with Colon Cancer Cells and Modulate their Invasive Phenotype

doi: 10.1016/j.tranon.2019.07.010

Figure Lengend Snippet: Differentiated human adipocytes. The cells were treated with the differentiation media provided by Zen-Bio. The photomicrographs show representative cells on day 14 of each treatment. (a), differentiated adipocytes from SC adipose tissue of a lean subject. (b), differentiated adipocytes from VS adipose tissue of a lean subject. (c), differentiated adipocytes from SC adipose tissue of an obese subject. (d), differentiated adipocytes from VS adipose tissue of an obese subject. Scale bar: 50 μm . Adipocytes at day 1 and 14 days after culturing in differentiation media were harvested and tested for the adipogenic transcriptional factor FABP4 using quantitative real-time PCR. Figure 1 E shows the results of the expression of FABP4 on day 14 as compared to day 1 of differentiation. ∗ P < .00001 (Student's t test).

Article Snippet: Zen-Bio is committed to provide highest quality isolated primary human cultured preadipocytes and adipocytes.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Translational Oncology

Article Title: Adipocytes Isolated from Visceral and Subcutaneous Depots of Donors Differing in BMI Crosstalk with Colon Cancer Cells and Modulate their Invasive Phenotype

doi: 10.1016/j.tranon.2019.07.010

Figure Lengend Snippet: Co-culture of colon cancer cells with mature adipocytes stimulates them to secrete more adipokines. Cancer cells (HM-7/HCT116), and visceral adipocytes (VA) or subcutaneous (SA) adipocytes from lean (L) or obese (F) subjects were co-cultured on Transwells or were separately cultured in 6 wells for 24 h. Conditioned media (CM) was collected and several adipokines were measured. (a) MCP1 (b) IL6 (c) IL8 (d) Leptin levels were presented as pg/mg total protein in cells. (a,b), # P < .001 (Student's t test) compared to co-cultured lean adipocytes and separately cultured F adipocytes, ∗ P < .05, compared to co-cultured obese adipocytes and separately cultured L adipocytes. (c), # P < .001 (Student's t test) compared to co-cultured lean adipocytes and separately cultured F adipocytes, ∗ P < .05, compared to co-cultured lean adipocytes and separately cultured F adipocytes. ⁎⁎ P < .05 (Student's t test) compared to separately cultured L adipocytes. (d), x P < .05, # P < .001 (Student's t test) compared to separately cultured L adipocytes, ∗ P < .05, ∗∗ P < .01 (Student's t test) compared to co-cultured lean adipocytes.

Article Snippet: Zen-Bio is committed to provide highest quality isolated primary human cultured preadipocytes and adipocytes.

Techniques: Co-Culture Assay, Cell Culture

Journal: Translational Oncology

Article Title: Adipocytes Isolated from Visceral and Subcutaneous Depots of Donors Differing in BMI Crosstalk with Colon Cancer Cells and Modulate their Invasive Phenotype

doi: 10.1016/j.tranon.2019.07.010

Figure Lengend Snippet: Cancer cells induce higher expression of adipokines from the different adipocytes. Gene expression was detected using quantitative real-time PCR in visceral adipocytes (a-d) or in subcutaneous adipocytes (e-h), from lean or obese subjects co-cultured with HM-7 cells. L + HM-7 represent gene expression in adipocytes from lean subject co-cultured with HM-7 cells. F + HM-7 represent gene expression in adipocytes from obese subject co-cultured with HM-7 cells. HM-7+ L (cancer cells) represent gene expression in HM-7 cells co-cultured with adipocytes from lean subject. HM-7+ F (cancer cells) represent gene expression in HM-7 cells co-cultured with adipocytes from obese subject. The results are presented as % of control (Lean (L) /obese (F)). ∗∗ P < .05, ∗ P < .01, # P < .001 (One way ANOVA- Dunnett's test).

Article Snippet: Zen-Bio is committed to provide highest quality isolated primary human cultured preadipocytes and adipocytes.

Techniques: Expressing, Gene Expression, Real-time Polymerase Chain Reaction, Cell Culture, Control

Journal: Translational Oncology

Article Title: Adipocytes Isolated from Visceral and Subcutaneous Depots of Donors Differing in BMI Crosstalk with Colon Cancer Cells and Modulate their Invasive Phenotype

doi: 10.1016/j.tranon.2019.07.010

Figure Lengend Snippet: CM from VA and SA of obese subjects increases the invasiveness ability of HM-7 cells. HM-7 cells (450 μl, 2.5 × 10 5 cells) were seeded on the top of Matrigel-coated transwell plate and CM from subcutaneous (a, c) or visceral (b, d) differentiated adipocytes were placed in the lower chamber. CM-F or CM-L represent HM-7 cells treated with conditioned media collected from wells containing only adipocytes from obese /lean subjects. The comparison was performed between this control and the effect of CM collected from co-cultured wells of adipocytes and HM-7 cancer cells (CM-F + HM7 or CM-L + HM-7). Invasion of HM-7 cells towards CM was assessed. Bars report mean fold change ± SEM. Results are presented as % of control. One of three experiments are presented. ∗∗ P < .05 vs. control (CM-L or F) (Student's t test).

Article Snippet: Zen-Bio is committed to provide highest quality isolated primary human cultured preadipocytes and adipocytes.

Techniques: Comparison, Control, Cell Culture

Journal: Translational Oncology

Article Title: Adipocytes Isolated from Visceral and Subcutaneous Depots of Donors Differing in BMI Crosstalk with Colon Cancer Cells and Modulate their Invasive Phenotype

doi: 10.1016/j.tranon.2019.07.010

Figure Lengend Snippet: FABP4 over expressed in HM-7 cells co-cultured with visceral adipocytes cells HM-7 (a, b) or HCT116 (c), cells co-cultured with visceral adipocytes (a,c) or subcutaneous adipocytes (b) from lean (HM-7/HCT116 + L) or obese subjects (HM-7/HCT116 + F). Twenty-four hours later cells were lysed and gene expression level was detected using quantitative real-time PCR. HM-7/HCT116 cells indicates control- cells which separately cultured, n = 3 wells from each treatment. Results are presented as % of control (HM-7/HCT116). ∗∗ P < .05, + P < .0001 (One way ANOVA-Dunnett's test).

Article Snippet: Zen-Bio is committed to provide highest quality isolated primary human cultured preadipocytes and adipocytes.

Techniques: Cell Culture, Gene Expression, Real-time Polymerase Chain Reaction, Control

Journal: Translational Oncology

Article Title: Adipocytes Isolated from Visceral and Subcutaneous Depots of Donors Differing in BMI Crosstalk with Colon Cancer Cells and Modulate their Invasive Phenotype

doi: 10.1016/j.tranon.2019.07.010

Figure Lengend Snippet: CM harvested from the co-culture system down-regulates the mitochondrial function of HCT116 cells (a-d), HCT116 (4×10 4 ) and (e-h), HM-7 cells (4.2×10 4 ) were treated for 24 hours with CM which were collected from: wells contained visceral adipocytes (a,b and e,f) or subcutaneous adipocytes (c,d and g,h) from obese and lean subjects and HCT116 or HM-7 colon cancer cells (CM-F/CM-L + HCT116, CM-F/CM-L + HM-7) or with CM which were collected from wells contained visceral adipocytes or subcutaneous adipocytes from obese (CM-F) or lean (CM-L) subjects which separately cultured. Cells were analyzed for their oxygen consumption rate (OCR) and maximal OCR following administration of 0.4 μM FCCP using the XF24 Analyzer. Cells which treated with regular adipocytes medium were used as control. n = 4 of each treatment. ∗∗ P < .05 vs. control (One-way ANOVE-Dunnett's test). Results are presented as % of control. Results of two independent experiments are shown.

Article Snippet: Zen-Bio is committed to provide highest quality isolated primary human cultured preadipocytes and adipocytes.

Techniques: Co-Culture Assay, Cell Culture, Control