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human holotransferrin  (R&D Systems)


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    Structured Review

    R&D Systems human holotransferrin
    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL <t>holotransferrin;</t> (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
    Human Holotransferrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+holotransferrin/Human+Holo-Transferrin+Protein%2C+CF/bio_rxiv__2025__06__22__660918-185-0-2
    Average 93 stars, based on 42 article reviews
    human holotransferrin - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Cell-type specific iron content regulation revealed by single-cell iron quantification"

    Article Title: Cell-type specific iron content regulation revealed by single-cell iron quantification

    Journal: bioRxiv

    doi: 10.1101/2025.06.22.660918

    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
    Figure Legend Snippet: (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.

    Techniques Used: Activity Assay

    (A). Mean fluorescence intensity (MFI) of CD71 (transferrin receptor) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (B). Mean fluorescence intensity (MFI) of CD25 (alpha chain component of the IL-2 surface receptor on T-cells) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (C). Mean fluorescence intensity (MFI) of CD71 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition; (D). Mean fluorescence intensity (MFI) of CD25 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition. Error bars present the 95 th confidence intervals for each metallomic distribution determined by sc-ICP-MS.
    Figure Legend Snippet: (A). Mean fluorescence intensity (MFI) of CD71 (transferrin receptor) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (B). Mean fluorescence intensity (MFI) of CD25 (alpha chain component of the IL-2 surface receptor on T-cells) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (C). Mean fluorescence intensity (MFI) of CD71 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition; (D). Mean fluorescence intensity (MFI) of CD25 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition. Error bars present the 95 th confidence intervals for each metallomic distribution determined by sc-ICP-MS.

    Techniques Used: Fluorescence

    Related Articles

    Bacteria:

    Article Title: Experimental Evidence of Bacterial Colonization of Human Coronary Microvasculature and Myocardial Tissue during Meningococcemia
    Article Snippet: .. Bacteria were then 156 concentrated by centrifugation and resuspended in physiological saline containing 20 mg/mL 157 of human holotransferrin to promote bacterial growth in vivo (2914HT, R&D systems) [18]. ..

    Article Title: Experimental Evidence of Bacterial Colonization of Human Coronary Microvasculature and Myocardial Tissue during Meningococcemia
    Article Snippet: .. Bacteria were then concentrated by centrifugation and resuspended in physiological saline containing 20 mg/ml of human holotransferrin to promote bacterial growth in vivo (2914HT; R&D Systems) ( 18 ). ..

    Centrifugation:

    Article Title: Experimental Evidence of Bacterial Colonization of Human Coronary Microvasculature and Myocardial Tissue during Meningococcemia
    Article Snippet: .. Bacteria were then 156 concentrated by centrifugation and resuspended in physiological saline containing 20 mg/mL 157 of human holotransferrin to promote bacterial growth in vivo (2914HT, R&D systems) [18]. ..

    Article Title: Experimental Evidence of Bacterial Colonization of Human Coronary Microvasculature and Myocardial Tissue during Meningococcemia
    Article Snippet: .. Bacteria were then concentrated by centrifugation and resuspended in physiological saline containing 20 mg/ml of human holotransferrin to promote bacterial growth in vivo (2914HT; R&D Systems) ( 18 ). ..

    Saline:

    Article Title: Experimental Evidence of Bacterial Colonization of Human Coronary Microvasculature and Myocardial Tissue during Meningococcemia
    Article Snippet: .. Bacteria were then 156 concentrated by centrifugation and resuspended in physiological saline containing 20 mg/mL 157 of human holotransferrin to promote bacterial growth in vivo (2914HT, R&D systems) [18]. ..

    Article Title: Experimental Evidence of Bacterial Colonization of Human Coronary Microvasculature and Myocardial Tissue during Meningococcemia
    Article Snippet: .. Bacteria were then concentrated by centrifugation and resuspended in physiological saline containing 20 mg/ml of human holotransferrin to promote bacterial growth in vivo (2914HT; R&D Systems) ( 18 ). ..

    In Vivo:

    Article Title: Experimental Evidence of Bacterial Colonization of Human Coronary Microvasculature and Myocardial Tissue during Meningococcemia
    Article Snippet: .. Bacteria were then 156 concentrated by centrifugation and resuspended in physiological saline containing 20 mg/mL 157 of human holotransferrin to promote bacterial growth in vivo (2914HT, R&D systems) [18]. ..

    Article Title: Experimental Evidence of Bacterial Colonization of Human Coronary Microvasculature and Myocardial Tissue during Meningococcemia
    Article Snippet: .. Bacteria were then concentrated by centrifugation and resuspended in physiological saline containing 20 mg/ml of human holotransferrin to promote bacterial growth in vivo (2914HT; R&D Systems) ( 18 ). ..

    Infection:

    Article Title: Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease.
    Article Snippet: 1U1016, Institut Cochin, Inserm, Paris, France.. 2UMR8104, CNRS, Paris, France.. 3Université Paris Descartes, Sorbonne Paris Cité, Paris, France.

    Stem Cell:

    Article Title: Single-cell RNA sequencing reveals that BMPR2 mutation regulates right ventricular function via ID genes
    Article Snippet: Peripheral blood mononuclear cell (PBMC) isolation and reprogramming PBMCs were isolated from peripheral blood (PB) using Ficoll-Paque Premium (GE Healthcare, cat. 17- 1440-03, UK) according to the method published by Bin-Kuan Chou et al. (1). .. By loading onto a 15 mL layer of Ficoll-Paque Premium, PBMCs were obtained from the mononuclear cell layer after being washed and seeded in low-attachment 6-well plates in MNC medium consisting of 50% IMDM (Gibco, cat. 21056- 023, USA), 50% Ham’s F12 medium (Gibco, cat. no. 31765-027), ITS-X (Gibco, cat. no. 51500-056), lipid concentrate (Gibco, cat. no. 11905031), 5 mg/mL BSA (Sigma-Aldrich, cat. no. A-9418, USA), 50 μg/mL L-ascorbic acid (Sigma, cat. no. A4403), 2 mM Glutamax (Gibco, cat. no. 35050), 200 μM 1-thioglycerol (Sigma, cat. no. no. M6145), 10 ng/mL interleukin (IL)-3 (PeproTech, cat. no. AF-200-03, USA), 100 ng/mL human stem cell factor (PeproTech, cat. no. AF-300-07), 40 ng/mL insulin-like growth factor 1 (PeproTech, cat. no. AF-100-11), 2 U/mL erythropoietin (R&D, cat. no.287-TC, USA), 100 mg/mL human holotransferrin (R&D, cat. no. 2914-HT), and 20 mM dexamethasone (Sigma, cat. no. D4902). ..



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    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL <t>holotransferrin;</t> (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
    Human Holotransferrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL <t>holotransferrin;</t> (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
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    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL <t>holotransferrin;</t> (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
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    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL <t>holotransferrin;</t> (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
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    Image Search Results


    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.

    Journal: bioRxiv

    Article Title: Cell-type specific iron content regulation revealed by single-cell iron quantification

    doi: 10.1101/2025.06.22.660918

    Figure Lengend Snippet: (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.

    Article Snippet: Human holotransferrin (R&D systems, 2914-HT-001G) was added at concentrations of 0.001 mg/mL to 0.625 mg/mL.

    Techniques: Activity Assay

    (A). Mean fluorescence intensity (MFI) of CD71 (transferrin receptor) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (B). Mean fluorescence intensity (MFI) of CD25 (alpha chain component of the IL-2 surface receptor on T-cells) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (C). Mean fluorescence intensity (MFI) of CD71 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition; (D). Mean fluorescence intensity (MFI) of CD25 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition. Error bars present the 95 th confidence intervals for each metallomic distribution determined by sc-ICP-MS.

    Journal: bioRxiv

    Article Title: Cell-type specific iron content regulation revealed by single-cell iron quantification

    doi: 10.1101/2025.06.22.660918

    Figure Lengend Snippet: (A). Mean fluorescence intensity (MFI) of CD71 (transferrin receptor) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (B). Mean fluorescence intensity (MFI) of CD25 (alpha chain component of the IL-2 surface receptor on T-cells) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (C). Mean fluorescence intensity (MFI) of CD71 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition; (D). Mean fluorescence intensity (MFI) of CD25 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition. Error bars present the 95 th confidence intervals for each metallomic distribution determined by sc-ICP-MS.

    Article Snippet: Human holotransferrin (R&D systems, 2914-HT-001G) was added at concentrations of 0.001 mg/mL to 0.625 mg/mL.

    Techniques: Fluorescence

    IMDM‐based Cellquin cell culture medium.

    Journal: HemaSphere

    Article Title: A cellular reporter system to evaluate endogenous fetal hemoglobin induction and screen for therapeutic compounds

    doi: 10.1002/hem3.139

    Figure Lengend Snippet: IMDM‐based Cellquin cell culture medium.

    Article Snippet: Human holotransferrin , 300 mg/L , Sanquin, NL via Mebiopharm, Tokyo, JP , NA.

    Techniques: Cell Culture, Concentration Assay, Recombinant