human holotransferrin  (ProSpec)

Journal: bioRxiv

Article Title: Rapid and precise quantification of lymphocyte iron content by single cell inductively coupled plasma mass spectrometry

doi: 10.1101/2024.11.11.623006

Figure Lengend Snippet: (A) . Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g); (C). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (D). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (E). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and r 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse. (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.

Article Snippet: Human holotransferrin (R&D systems, 2914-HT-001G) was added at concentrations of 0.001 mg/mL to 0.625 mg/mL.

Techniques: Activity Assay

Journal: bioRxiv

Article Title: Rapid and precise quantification of lymphocyte iron content by single cell inductively coupled plasma mass spectrometry

doi: 10.1101/2024.11.11.623006

Figure Lengend Snippet: (A) . Mean fluorescence intensity (MFI) of CD71 (transferrin receptor) versus the geometric mean single cell iron content (ag) for each population distribution, from each holotransferrin condition; (B). Mean fluorescence intensity (MFI) of CD25 (alpha chain component of the IL-2 surface receptor on T-cells) versus the geometric mean single cell iron content (ag) for each population distribution, from each holotransferrin condition; (C). Mean fluorescence intensity (MFI) of CD71 versus the geometric mean single cell calcium content (ag) for each population distribution, from each holotransferrin condition; (D). Mean fluorescence intensity (MFI) of CD25 versus the geometric mean single cell calcium content (ag) for each population distribution, from each holotransferrin condition. Error bars present the 95 th confidence intervals for each metallomic distribution determined by SC-ICP-MS.

Article Snippet: Human holotransferrin (R&D systems, 2914-HT-001G) was added at concentrations of 0.001 mg/mL to 0.625 mg/mL.

Techniques: Fluorescence

Journal: HemaSphere

Article Title: A cellular reporter system to evaluate endogenous fetal hemoglobin induction and screen for therapeutic compounds

doi: 10.1002/hem3.139

Figure Lengend Snippet: IMDM‐based Cellquin cell culture medium.

Article Snippet: Human holotransferrin , 300 mg/L , Sanquin, NL via Mebiopharm, Tokyo, JP , NA.

Techniques: Cell Culture, Concentration Assay, Recombinant