human hela cell line  (ATCC)

Journal: Journal of Cell Science

Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression

doi: 10.1242/jcs.264572

Figure Lengend Snippet: Hypoxia increases expression of EZH2, H3K27me3 and survivin. (A) Immunoblots of WCEs from U2OS, HeLa and MRC5 lines cultured under normoxic or hypoxic environments (24 h). Blots were immunoprobed with anti-EZH2, anti-H3K27me3 and anti-survivin antibodies. Anti-Hif1a used to prove the hypoxic state had been induced, and anti-tubulin was used as a loading control. (B–D) Quantification of immunoblots represented in A from three independent experiments demonstrating that EZH2, H3K27me3 and survivin are all more abundant under hypoxia. Data presented are means±s.d. * P <0.05, ** P <0.01, *** P <0.001 (two-way ANOVA with Tukey's multiple comparisons post test).

Article Snippet: Human cervical cancer cells (HeLa), human bone osteosarcoma cells (U2OS), retinal pigment epithelial cells (RPE) and human breast cancer cells (MCF7) were originally from ATCC.

Techniques: Expressing, Western Blot, Cell Culture, Control

Journal: RSC Advances

Article Title: An approach for the systematic profiling of drug-induced remodeling of RNA–RBP (RNA-binding protein) interactions

doi: 10.1039/d6ra00871b

Figure Lengend Snippet: Characterization and optimization of the RPC enrichment process. (a) Dot blot analysis detecting BFP labeling of the RNA extracted from HeLa cells under 365 nm UV irradiation. SDS-PAGE characterization of enriched RBPs under different conditions, including 254 nm UV irradiation dose (b), BFP concentration (c), and 365 nm UV labeling time (d).

Article Snippet: Human epithelial (HeLa), human colon cancer (HCT116) and human embryonic kidney (HEK293T) cell lines were obtained from the American Type Culture Collection (ATCC).

Techniques: Dot Blot, Labeling, Irradiation, SDS Page, Concentration Assay