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human hbegf  (R&D Systems)


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    R&D Systems human hbegf
    Human Hbegf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hbegf/Human+HB-EGF+Antibody/pm41181988-391-25-28
    Average 93 stars, based on 3 article reviews
    human hbegf - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: Therapeutic Reprogramming of Glioblastoma Phenotypic States Using Multifunctional Heparin Nanoparticles
    Article Snippet: Monoclonal mouse anti‐human vimentin Cy3 conjugate (C9080, MilliporeSigma, 1:900); monoclonal rat anti‐mouse podocalyxin (MAB1556, R&D Systems, Bio‐Techne, 1:800) and mouse monoclonal anti human HBEGF (MAB2591, R&D Systems 1:200)

    Cell Culture:

    Article Title: Therapeutic Reprogramming of Glioblastoma Phenotypic States Using Multifunctional Heparin Nanoparticles.
    Article Snippet: Antibodies and Reagents—Western Blotting: Monoclonal mouse antiEGFR (sc-373746, Santa Cruz, 1:1000), monoclonal mouse anti-HSC70 (sc-7298, Santa Cruz, 1:1000), monoclonal mouse anti GFAP (G3893, Sigma-Aldrich, 1:1000), polyclonal rabbit anti-meteorin (600-430, ThermoFisher, 1:500), monoclonal mouse anti human vimentin (C9080, Sigma-Aldrich, 1:1000), monoclonal mouse anti GFAP (G3893, SigmaAldrich, 1:1000), and monoclonal mouse anti human HBEGF (sc-74441, Santa Cruz, 1:1000). .. Antibodies and Reagents—Immunofluorescence: Monoclonal mouse anti-human vimentin Cy3 conjugate (C9080, MilliporeSigma, 1:900); monoclonal rat anti-mouse podocalyxin (MAB1556, R&D Systems, Bio-Techne, 1:800) and mouse monoclonal anti human HBEGF (MAB2591, R&D Systems 1:200) Cell Culture: Isolation of patient-derived GSCs (BT3, BT12, BT13, BT27, ZH305, and S24) was previously described. .. [41] Patient-derived glioma cell lines were maintained in serum-free DMEM/F12 medium (BTs lines) or Neurobasal (ZH305, S24) supplemented with 1× B27 (both from Gibco, Thermo Fisher Scientific), 2mm l-glutamine, 100 UmL−1 penicillin, 100 μg mL−1 streptomycin, 15 mMHEPES (all from Lonza), 0.02 μg mL−1 human EGF, and 0.01 μg mL−1 human FGF-basic (both from PeproTech).

    Isolation:

    Article Title: Therapeutic Reprogramming of Glioblastoma Phenotypic States Using Multifunctional Heparin Nanoparticles.
    Article Snippet: Antibodies and Reagents—Western Blotting: Monoclonal mouse antiEGFR (sc-373746, Santa Cruz, 1:1000), monoclonal mouse anti-HSC70 (sc-7298, Santa Cruz, 1:1000), monoclonal mouse anti GFAP (G3893, Sigma-Aldrich, 1:1000), polyclonal rabbit anti-meteorin (600-430, ThermoFisher, 1:500), monoclonal mouse anti human vimentin (C9080, Sigma-Aldrich, 1:1000), monoclonal mouse anti GFAP (G3893, SigmaAldrich, 1:1000), and monoclonal mouse anti human HBEGF (sc-74441, Santa Cruz, 1:1000). .. Antibodies and Reagents—Immunofluorescence: Monoclonal mouse anti-human vimentin Cy3 conjugate (C9080, MilliporeSigma, 1:900); monoclonal rat anti-mouse podocalyxin (MAB1556, R&D Systems, Bio-Techne, 1:800) and mouse monoclonal anti human HBEGF (MAB2591, R&D Systems 1:200) Cell Culture: Isolation of patient-derived GSCs (BT3, BT12, BT13, BT27, ZH305, and S24) was previously described. .. [41] Patient-derived glioma cell lines were maintained in serum-free DMEM/F12 medium (BTs lines) or Neurobasal (ZH305, S24) supplemented with 1× B27 (both from Gibco, Thermo Fisher Scientific), 2mm l-glutamine, 100 UmL−1 penicillin, 100 μg mL−1 streptomycin, 15 mMHEPES (all from Lonza), 0.02 μg mL−1 human EGF, and 0.01 μg mL−1 human FGF-basic (both from PeproTech).

    Recombinant:

    Article Title: Heparin-binding epidermal growth factor-like growth factor in experimental models of membranous and minimal change nephropathy.
    Article Snippet: .. Firstly, four New Zealand white rabbits were immunized subcutaneously with recombinant human HBEGF (R&D Systems, Minneapolis, MN, USA), using 5 mg per immunization mixed with complete Freund’s adjuvant on the first occasion, and incomplete Freund’s adjuvant on a further three occasions at four weekly intervals. .. Following ELISA and immunohistochemical assays of multiple test bleeds, serum from one of the animals (R847) was incubated at 56°C for 45 minutes to inactivate complement, diluted in PBS, and affinity purified on a Protein G Sepharose column (Sigma).

    Adjuvant:

    Article Title: Heparin-binding epidermal growth factor-like growth factor in experimental models of membranous and minimal change nephropathy.
    Article Snippet: .. Firstly, four New Zealand white rabbits were immunized subcutaneously with recombinant human HBEGF (R&D Systems, Minneapolis, MN, USA), using 5 mg per immunization mixed with complete Freund’s adjuvant on the first occasion, and incomplete Freund’s adjuvant on a further three occasions at four weekly intervals. .. Following ELISA and immunohistochemical assays of multiple test bleeds, serum from one of the animals (R847) was incubated at 56°C for 45 minutes to inactivate complement, diluted in PBS, and affinity purified on a Protein G Sepharose column (Sigma).

    Transduction:

    Article Title: Distinct Mechanisms of Receptor and Nonreceptor Tyrosine Kinase Activation by Reactive Oxygen Species in Vascular Smooth Muscle Cells: Role of Metalloprotease and Protein Kinase C-δ
    Article Snippet: H2O2, AngII, N-acetylcysteine, and poly[Glu80-Tyr20] were purchased from Sigma. .. Antibodies were purchased from the following sources: phospho-JAK2, phospho-EGF receptor, and phospho-PYK2, BioSource International; JAK2, Upstate Biotechnology; EGF receptor, PKC- , PKC- , and PKC- 1, Santa Cruz Biotechnology; PYK2, Transduction Laboratories; and neutralizing human HBEGF, R & D Systems. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Cross-Talk between Estrogen Receptor and Epidermal Growth Factor Receptor in Head and Neck Squamous Cell Carcinoma
    Article Snippet: M225 was obtained from Imclone Systems, Inc. Marimistat was obtained from British Biotech. .. TGFα Quantikine ELISA kit, human HBEGF and amphiregulin DuoSet ELISA kits, and amphiregulin NA were from R&D Systems. .. HB-EGF NA was from Calbiochem.

    Saline:

    Article Title: Cellular reprogramming into a brown adipose tissue-like phenotype by co-expression of HB-EGF and ADAM 12S.
    Article Snippet: Abnormal adipogenesis leads to excessive fat accumulation and several health disorders.. Mouse fibroblasts (MLC) transfected with ADAM 12S and HB-EGF promoted lipid accumulation.. Addition of KBR-7785, an ADAM 12S inhibitor, to HB-EGF/ADAM 12S expressing cells suppressed adipogenesis.

    Gentle:

    Article Title: Cellular reprogramming into a brown adipose tissue-like phenotype by co-expression of HB-EGF and ADAM 12S.
    Article Snippet: Abnormal adipogenesis leads to excessive fat accumulation and several health disorders.. Mouse fibroblasts (MLC) transfected with ADAM 12S and HB-EGF promoted lipid accumulation.. Addition of KBR-7785, an ADAM 12S inhibitor, to HB-EGF/ADAM 12S expressing cells suppressed adipogenesis.

    Incubation:

    Article Title: Cellular reprogramming into a brown adipose tissue-like phenotype by co-expression of HB-EGF and ADAM 12S.
    Article Snippet: Abnormal adipogenesis leads to excessive fat accumulation and several health disorders.. Mouse fibroblasts (MLC) transfected with ADAM 12S and HB-EGF promoted lipid accumulation.. Addition of KBR-7785, an ADAM 12S inhibitor, to HB-EGF/ADAM 12S expressing cells suppressed adipogenesis.



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    The effect of IL‐1β release of HBEGF and secretion of MMP10 by endothelial cells in culture. (A) EC (0.5 × 10 6 per dish) were incubated in 0% (v/v) FCS media for 72 h with 5 ng/mL IL‐1β with or without 100 mM NNGH. The concentration of HBEGF was determined by ELISA and corrected for cellular protein. The results are expressed as fold change in HBEGF and is the mean ± SEM of n = 4 independent experiments. The level of significance was determined using one‐way ANOVA with Sidak's multiple comparisons test where ns, not significant, **** p < 0.0005. (B) rh‐MMP3 and rh‐MMP10 (2.5‐10 ng/well) was subjected to PAGE, transferred and probed with the R&D systems antibody to determine specificity. (C) EC (0.75 × 10 6 cells per dish) were cultured for 48 and the medium then removed and concentrated as detailed in the methods. Lane 1 is the molecular size ladder lane 2 control cells, lane 3 cells stimulated with 10 ng/mL IL1β and lane 4 is recombinant MMP10. MMP10 was detected by chemiluminescence and is representative of n = 3 independent experiments. The molecular weight of bands obtained in this experiment were calculated using Image Lab v6.1 by BioRad.

    Journal: The FASEB Journal

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    doi: 10.1096/fj.202402329RR

    Figure Lengend Snippet: The effect of IL‐1β release of HBEGF and secretion of MMP10 by endothelial cells in culture. (A) EC (0.5 × 10 6 per dish) were incubated in 0% (v/v) FCS media for 72 h with 5 ng/mL IL‐1β with or without 100 mM NNGH. The concentration of HBEGF was determined by ELISA and corrected for cellular protein. The results are expressed as fold change in HBEGF and is the mean ± SEM of n = 4 independent experiments. The level of significance was determined using one‐way ANOVA with Sidak's multiple comparisons test where ns, not significant, **** p < 0.0005. (B) rh‐MMP3 and rh‐MMP10 (2.5‐10 ng/well) was subjected to PAGE, transferred and probed with the R&D systems antibody to determine specificity. (C) EC (0.75 × 10 6 cells per dish) were cultured for 48 and the medium then removed and concentrated as detailed in the methods. Lane 1 is the molecular size ladder lane 2 control cells, lane 3 cells stimulated with 10 ng/mL IL1β and lane 4 is recombinant MMP10. MMP10 was detected by chemiluminescence and is representative of n = 3 independent experiments. The molecular weight of bands obtained in this experiment were calculated using Image Lab v6.1 by BioRad.

    Article Snippet: HB‐EGF was detected by ELISA using an HBEGF Duo Set (R&D Systems UK Cat#DY259B).

    Techniques: Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Control, Recombinant, Molecular Weight