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c human β glucuronidase enzyme  (R&D Systems)


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    R&D Systems c human β glucuronidase enzyme
    C Human β Glucuronidase Enzyme, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+gh/Recombinant+Human+beta-Glucuronidase%2FGUSB+Protein%2C+CF/us12576156-1930-14-18
    Average 94 stars, based on 6 article reviews
    c human β glucuronidase enzyme - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Recovery using "float" from high intensity stress on growth hormone-like molecules in resistance trained men.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Oxygen-sensitive regulation and neuroprotective effects of growth hormone-dependent growth factors during early postnatal development.
    Article Snippet: Jung S, Boie G, Doerr H, Trollmann R. Oxygen-sensitive regulation and neuroprotective effects of growth hormone-dependent growth factors during early postnatal development.. Am J Physiol Regul Integr Comp Physiol 312: R539–R548, 2017.. First published February 22, 2017; doi:10.1152/ajpregu.00477.2016.—Perinatal hypoxia severely disrupts metabolic and somatotrophic development, as well as cerebral maturational programs.

    Article Title: Response of the Insulin-Like Growth Factor (IGF) System to IGF-IR Inhibition and Androgen Deprivation in a Neoadjuvant Prostate Cancer Trial: Effects of Obesity and Androgen Deprivation
    Article Snippet: .. Serum levels of human GH were determined by commercial ELISA kit (R&D Systems, Minneapolis, Minnesota) according to the manufacturer's instructions similar to above. ..

    Clinical Proteomics:

    Article Title: Oxygen-sensitive regulation and neuroprotective effects of growth hormone-dependent growth factors during early postnatal development.
    Article Snippet: Jung S, Boie G, Doerr H, Trollmann R. Oxygen-sensitive regulation and neuroprotective effects of growth hormone-dependent growth factors during early postnatal development.. Am J Physiol Regul Integr Comp Physiol 312: R539–R548, 2017.. First published February 22, 2017; doi:10.1152/ajpregu.00477.2016.—Perinatal hypoxia severely disrupts metabolic and somatotrophic development, as well as cerebral maturational programs.

    Article Title: Oral Supplementation Using Gamma-Aminobutyric Acid and Whey Protein Improves Whole Body Fat-Free Mass in Men After Resistance Training
    Article Snippet: .. Plasma GH concentrations were measured using a commercially available enzyme-linked immunosorbent assay for human GH (R&D Systems, Minneapolis, MN, USA). ..

    Cell Culture:

    Article Title: Increased serum and bone matrix levels of transforming growth factor β1 in patients with GH deficiency in response to GH treatment
    Article Snippet: In study 2, total body composition including total lean mass, fat mass, and bone mineral density was measured by DEXA (DPX-L, software version 1.31; Lunar Corp., Madison, WI, USA). .. The hFOB cell line 1.19 was obtained from American Type Culture Collection (Rockville, MD, USA) and cultured as described previously (15). hFOB cells were differentiated for 2 days, followed by culturing with and without recombinant human GH and IGF1 (R&D Systems, Minneapolis, MN, USA) for 3, 6, and 24 h. In some experiments, a blocking antibody against the IGF1 receptor (IGF1R) was used (concentration 20 mg/ml, R&D Systems). .. The primary human subcutaneous and visceral preadipocytes (Lonza Walkersville, Inc., Walkersville, MD, USA) were cultured in preadipocyte basal medium-2 (PBM-2; Lonza) supplemented with FCS (10%), glutamine (2 mM), penicillin (100 IU/ml), and streptomycin (100 mg/ml).

    Recombinant:

    Article Title: Increased serum and bone matrix levels of transforming growth factor β1 in patients with GH deficiency in response to GH treatment
    Article Snippet: In study 2, total body composition including total lean mass, fat mass, and bone mineral density was measured by DEXA (DPX-L, software version 1.31; Lunar Corp., Madison, WI, USA). .. The hFOB cell line 1.19 was obtained from American Type Culture Collection (Rockville, MD, USA) and cultured as described previously (15). hFOB cells were differentiated for 2 days, followed by culturing with and without recombinant human GH and IGF1 (R&D Systems, Minneapolis, MN, USA) for 3, 6, and 24 h. In some experiments, a blocking antibody against the IGF1 receptor (IGF1R) was used (concentration 20 mg/ml, R&D Systems). .. The primary human subcutaneous and visceral preadipocytes (Lonza Walkersville, Inc., Walkersville, MD, USA) were cultured in preadipocyte basal medium-2 (PBM-2; Lonza) supplemented with FCS (10%), glutamine (2 mM), penicillin (100 IU/ml), and streptomycin (100 mg/ml).

    Blocking Assay:

    Article Title: Increased serum and bone matrix levels of transforming growth factor β1 in patients with GH deficiency in response to GH treatment
    Article Snippet: In study 2, total body composition including total lean mass, fat mass, and bone mineral density was measured by DEXA (DPX-L, software version 1.31; Lunar Corp., Madison, WI, USA). .. The hFOB cell line 1.19 was obtained from American Type Culture Collection (Rockville, MD, USA) and cultured as described previously (15). hFOB cells were differentiated for 2 days, followed by culturing with and without recombinant human GH and IGF1 (R&D Systems, Minneapolis, MN, USA) for 3, 6, and 24 h. In some experiments, a blocking antibody against the IGF1 receptor (IGF1R) was used (concentration 20 mg/ml, R&D Systems). .. The primary human subcutaneous and visceral preadipocytes (Lonza Walkersville, Inc., Walkersville, MD, USA) were cultured in preadipocyte basal medium-2 (PBM-2; Lonza) supplemented with FCS (10%), glutamine (2 mM), penicillin (100 IU/ml), and streptomycin (100 mg/ml).

    Concentration Assay:

    Article Title: Increased serum and bone matrix levels of transforming growth factor β1 in patients with GH deficiency in response to GH treatment
    Article Snippet: In study 2, total body composition including total lean mass, fat mass, and bone mineral density was measured by DEXA (DPX-L, software version 1.31; Lunar Corp., Madison, WI, USA). .. The hFOB cell line 1.19 was obtained from American Type Culture Collection (Rockville, MD, USA) and cultured as described previously (15). hFOB cells were differentiated for 2 days, followed by culturing with and without recombinant human GH and IGF1 (R&D Systems, Minneapolis, MN, USA) for 3, 6, and 24 h. In some experiments, a blocking antibody against the IGF1 receptor (IGF1R) was used (concentration 20 mg/ml, R&D Systems). .. The primary human subcutaneous and visceral preadipocytes (Lonza Walkersville, Inc., Walkersville, MD, USA) were cultured in preadipocyte basal medium-2 (PBM-2; Lonza) supplemented with FCS (10%), glutamine (2 mM), penicillin (100 IU/ml), and streptomycin (100 mg/ml).



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    Image Search Results


    PMN antifungal activity in VCM spiked with HS or treated with HPSEs. Vaginal swab samples were collected from three groups: women with RVVC experiencing a symptomatic acute episode of VVC (symptomatic group, n = 31), those in asymptomatic remission (asymptomatic group, n = 14), and healthy women without a history of frequent VVC (control group, n = 16). The data sets included an additional five follow-up swabs collected from women in the symptomatic and asymptomatic groups. Detached swab tips were suspended individually in 1 mL RPMI 1640 medium to release vaginal secretions and cellular content. Supernatants were sterile-filtered as VCM. PMNs (5 × 10 5 ) isolated from peripheral blood of healthy volunteers were incubated with C. albicans cells (1 × 10 5 ) in 100 µL of VCM or RPMI medium for 3 h at 37°C with 5% CO 2 . Controls consisted of C. albicans cultured alone in VCM or RPMI medium and were used to calculate % killing. ( A ) VCM samples with normal killing activity (non-inhibitory VCM, gray bar) were spiked with purified porcine HS (500 µg/mL) and reevaluated for PMN killing activity (blue bar). Spiked non-inhibitory VCM was then pretreated with r Ph HPSE (3 ng/mL) and reevaluated for PMN killing activity (red bar). ( B ) VCM samples with low killing activity (inhibitory VCM, solid blue bar) were pretreated with rhHPSE (4 µg/mL, red-dotted bar) or r Ph HPSE (3 ng/mL, red-striped bar) and reevaluated for PMN killing activity. After coculture, viable C. albicans cells were enumerated by quantitative plate counts. Data were analyzed using the one-way ANOVA and Bonferroni’s post-test. Bar heights and error bars represent group means ± SEM for % killing values across independent replicates of unique VCM samples. The graphs represent cumulative data from three to four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

    Journal: Infection and Immunity

    Article Title: Elevated vaginal heparan sulfate correlates with impaired neutrophil killing of Candida albicans in women with vulvovaginal candidiasis

    doi: 10.1128/iai.00709-25

    Figure Lengend Snippet: PMN antifungal activity in VCM spiked with HS or treated with HPSEs. Vaginal swab samples were collected from three groups: women with RVVC experiencing a symptomatic acute episode of VVC (symptomatic group, n = 31), those in asymptomatic remission (asymptomatic group, n = 14), and healthy women without a history of frequent VVC (control group, n = 16). The data sets included an additional five follow-up swabs collected from women in the symptomatic and asymptomatic groups. Detached swab tips were suspended individually in 1 mL RPMI 1640 medium to release vaginal secretions and cellular content. Supernatants were sterile-filtered as VCM. PMNs (5 × 10 5 ) isolated from peripheral blood of healthy volunteers were incubated with C. albicans cells (1 × 10 5 ) in 100 µL of VCM or RPMI medium for 3 h at 37°C with 5% CO 2 . Controls consisted of C. albicans cultured alone in VCM or RPMI medium and were used to calculate % killing. ( A ) VCM samples with normal killing activity (non-inhibitory VCM, gray bar) were spiked with purified porcine HS (500 µg/mL) and reevaluated for PMN killing activity (blue bar). Spiked non-inhibitory VCM was then pretreated with r Ph HPSE (3 ng/mL) and reevaluated for PMN killing activity (red bar). ( B ) VCM samples with low killing activity (inhibitory VCM, solid blue bar) were pretreated with rhHPSE (4 µg/mL, red-dotted bar) or r Ph HPSE (3 ng/mL, red-striped bar) and reevaluated for PMN killing activity. After coculture, viable C. albicans cells were enumerated by quantitative plate counts. Data were analyzed using the one-way ANOVA and Bonferroni’s post-test. Bar heights and error bars represent group means ± SEM for % killing values across independent replicates of unique VCM samples. The graphs represent cumulative data from three to four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

    Article Snippet: To degrade HS, symptomatic (inhibitory) VCM was pretreated with either recombinant human HPSE (rhHPSE, 4 μg/mL, R&D Systems) or recombinant Pedobacter heparinus heparinase III (r Ph HPSE, 3 ng/mL, R&D Systems) for 1 h at 37°C.

    Techniques: Activity Assay, Control, Sterility, Isolation, Incubation, Cell Culture, Purification