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tpa elisa kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology tpa elisa kit
    Tpa Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+elisa+kit/Human+tPA+(Tissue-type+Plasminogen+Activator)+ELISA+Kit/pm42909374-85-9-12
    Average 93 stars, based on 6 article reviews
    tpa elisa kit - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Bitter frankincense water modulates hepatic enzyme activity in female patients with irritable bowel syndrome.
    Article Snippet: .. Human ELISA Kit, the manufacturing company (Elabscience company) for measuring human alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in serum, while the human ELISA Kit alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT) was supplied by (Fine Biotech Co., Ltd) for measuring their levels in serum, According to the working method used in the kit prepared by the companies. ..

    Article Title: VISTA expressed on tumor cells is regulated by m6A and influences immune microenvironment through STAT3/CCL22 in NSCLC
    Article Snippet: .. Specific secretory proteins were detected using human ELISA Kit (E-EL-H0029, Elabscience) and mouse ELISA Kit (E-EL-M0014), following the manufacturer’s instructions. ..

    Article Title: The Influence of Mat Pilates Training on Cardiometabolic Risk Factors in Postmenopausal Women with Single or Multiple Cardiometabolic Diseases
    Article Snippet: .. All analyses were conducted with an automated system (Cobas Mira, Roche Instruments Inc., Brookhaven, NY, USA), except for adiponectin, which was measured using a human ELISA kit (ELABSCIENCE, Wuhan, China). ..

    Article Title: Salivary Cystatin C as a Potential Biomarker in Stage III/IV Periodontitis: A Comparative Study
    Article Snippet: .. Cystatin C levels were measured using a commercially available human ELISA kit (Elabscience® Human Cys-C (Cystatin C) ELISA Kit). ..

    Article Title: From stage 1 to end-stage renal failure: Amyloid β42, amyloid β40, amyloid β42/40 ratio, p-tau181 and cognitive function relationship.
    Article Snippet: .. The serum Aβ1–42 (Catalogue No: E-EL-H0543), Aβ1–40 (Catalogue No: E-EL-H0542) levels were analyzed by using commercial human ELISA kit (Elabscience Biotechnology, Shanghai, China), and the serum T181 (Catalogue No: E7668Hu) levels by using a commercial human ELISA kit (BT LAB, Zhejiang, PRC) according to the manufacturer’s instructions. .. The absorbance of all wells was measured with the CLARIOstar Microplate Reader (BMG LABTECH, Ortenberg, Germany) at 450 nm.

    Article Title: Irisin, a promising adipomyokine, shows low levels in relation to periodontal diseases, unlike visfatin and IL-6 (case-control study)
    Article Snippet: After being centrifuged at 10,000 rpm for 10 min, all saliva samples were transferred into Eppendorf tubes and stored at −20°C until the analysis day ( ). .. To measure irisin, visfatin, and IL-6 levels, we utilized specialized ELISA kits (Human ELISA Kit, Elabscience) according to the manufacturer's instructions. .. Salivary biomarkers were quantified using commercially available ELISA kits (Elabscience®, Houston, TX, USA) according to the manufacturer's instructions: Human Irisin (Catalog No: E-EL-H6120; detection range: 15.63–1,000 pg/ml; sensitivity: 9.38 pg/ml), Human Interleukin-6 (IL-6) (Catalog No: E-EL-H0102c; detection range: 7.81–500 pg/ml; sensitivity: 4.69 pg/ml), and Human Visfatin (Catalog No: E-EL-H1330c; detection range: 0.313–20 ng/ml; sensitivity: 0.19 ng/ml).

    Article Title: The Influence of Mat Pilates Training on Cardiometabolic Risk Factors in Postmenopausal Women with Single or Multiple Cardiometabolic Diseases
    Article Snippet: .. All analyses were conducted with an automated system (Cobas Mira, Roche Instruments Inc., Brookhaven, NY, USA), except for adiponectin, which was measured using a human ELISA kit (ELABSCIENCE, Wuhan, China). ..

    Article Title: VISTA expressed on tumor cells is regulated by m6A and influences immune microenvironment through STAT3/CCL22 in NSCLC.
    Article Snippet: .. Specific secretory proteins were detected using human ELISA Kit (E-EL-H0029, Elabscience) and mouse ELISA Kit (E-ELM0014), following the manufacturer’s instructions. m6A Dot blot Spot 125 ng, 250 ng, and 500 ng of mRNA onto a nylon membrane and allow it to dry in the dark. ..

    AST Assay:

    Article Title: Bitter frankincense water modulates hepatic enzyme activity in female patients with irritable bowel syndrome.
    Article Snippet: .. Human ELISA Kit, the manufacturing company (Elabscience company) for measuring human alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in serum, while the human ELISA Kit alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT) was supplied by (Fine Biotech Co., Ltd) for measuring their levels in serum, According to the working method used in the kit prepared by the companies. ..

    Dot Blot:

    Article Title: VISTA expressed on tumor cells is regulated by m6A and influences immune microenvironment through STAT3/CCL22 in NSCLC.
    Article Snippet: .. Specific secretory proteins were detected using human ELISA Kit (E-EL-H0029, Elabscience) and mouse ELISA Kit (E-ELM0014), following the manufacturer’s instructions. m6A Dot blot Spot 125 ng, 250 ng, and 500 ng of mRNA onto a nylon membrane and allow it to dry in the dark. ..

    Membrane:

    Article Title: VISTA expressed on tumor cells is regulated by m6A and influences immune microenvironment through STAT3/CCL22 in NSCLC.
    Article Snippet: .. Specific secretory proteins were detected using human ELISA Kit (E-EL-H0029, Elabscience) and mouse ELISA Kit (E-ELM0014), following the manufacturer’s instructions. m6A Dot blot Spot 125 ng, 250 ng, and 500 ng of mRNA onto a nylon membrane and allow it to dry in the dark. ..



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    Epinephrine and/or chlorpromazine eliminate APAP-induced oxidative stress and inflammation and modulate the expression of the thioredoxin system in the liver. ( A ) and ( B ) demonstrate the hepatic levels of Nrf2 and TNF-α, respectively. ( C ), ( D ), and ( E ) show the effect of EP and CPZ on the thioredoxin system <t>(Trx1/TrxR1)</t> ( C ), thioredoxin-related proteins (TrxL1 and TrxIP) ( D ) mRNA and TrxR1 protein ( E ). Bars represent the mean (± SD) of 3 experiments. Means are compared by two-way ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, *** p < 0.0001, **** p < 0.0001, and “ns” is not significant
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    Image Search Results


    Epinephrine and/or chlorpromazine eliminate APAP-induced oxidative stress and inflammation and modulate the expression of the thioredoxin system in the liver. ( A ) and ( B ) demonstrate the hepatic levels of Nrf2 and TNF-α, respectively. ( C ), ( D ), and ( E ) show the effect of EP and CPZ on the thioredoxin system (Trx1/TrxR1) ( C ), thioredoxin-related proteins (TrxL1 and TrxIP) ( D ) mRNA and TrxR1 protein ( E ). Bars represent the mean (± SD) of 3 experiments. Means are compared by two-way ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, *** p < 0.0001, **** p < 0.0001, and “ns” is not significant

    Journal: BMC Pharmacology & Toxicology

    Article Title: Epinephrine-mediated sensitization of thioredoxin 1 enhances chlorpromazine protection against acute hepatotoxicity

    doi: 10.1186/s40360-026-01225-w

    Figure Lengend Snippet: Epinephrine and/or chlorpromazine eliminate APAP-induced oxidative stress and inflammation and modulate the expression of the thioredoxin system in the liver. ( A ) and ( B ) demonstrate the hepatic levels of Nrf2 and TNF-α, respectively. ( C ), ( D ), and ( E ) show the effect of EP and CPZ on the thioredoxin system (Trx1/TrxR1) ( C ), thioredoxin-related proteins (TrxL1 and TrxIP) ( D ) mRNA and TrxR1 protein ( E ). Bars represent the mean (± SD) of 3 experiments. Means are compared by two-way ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, *** p < 0.0001, **** p < 0.0001, and “ns” is not significant

    Article Snippet: Reduced Glutathione (GSH) Colorimetric Assay Kit (Cat. No. E-BC-K030-M), Human Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2) ELISA Kit (Cat No. E-EL-H1564), Human TrxR1 (Thioredoxin Reductase 1) ELISA Kit (Cat. No. E-EL-H1728), human TNF-α ELISA Kit (Cat. No. E-EL-H0109) were from Elabscience, Houston, Tx, USA.

    Techniques: Expressing

    Epinephrine and EGCG effect on cell viability, Nrf2, NTF, and TrxR1 expression in hepatoma cells. HepG2 and Huh7 cells were treated with epinephrine ( A ) or EGCG ( B ) for 24 h, after which cell metabolic activity was determined by MTT assay. Epinephrine enhanced cell viability in both HepG2 and Huh7 cells, whereas EGCG reduced cell viability. ( C ), ( D ), and ( E ) show the protein levels of Nrf2, TNF-α, and TrxR1, respectively, assessed in cells treated with EP, EGCG, CPZ, EP/CPZ, or EGCG/CPZ. Bars represent the mean (± SD) of 3 experiments. Groups were compared using ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, (***): p < 0.001, (****): p < 0.0001, and “ns” is not significant

    Journal: BMC Pharmacology & Toxicology

    Article Title: Epinephrine-mediated sensitization of thioredoxin 1 enhances chlorpromazine protection against acute hepatotoxicity

    doi: 10.1186/s40360-026-01225-w

    Figure Lengend Snippet: Epinephrine and EGCG effect on cell viability, Nrf2, NTF, and TrxR1 expression in hepatoma cells. HepG2 and Huh7 cells were treated with epinephrine ( A ) or EGCG ( B ) for 24 h, after which cell metabolic activity was determined by MTT assay. Epinephrine enhanced cell viability in both HepG2 and Huh7 cells, whereas EGCG reduced cell viability. ( C ), ( D ), and ( E ) show the protein levels of Nrf2, TNF-α, and TrxR1, respectively, assessed in cells treated with EP, EGCG, CPZ, EP/CPZ, or EGCG/CPZ. Bars represent the mean (± SD) of 3 experiments. Groups were compared using ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, (***): p < 0.001, (****): p < 0.0001, and “ns” is not significant

    Article Snippet: Reduced Glutathione (GSH) Colorimetric Assay Kit (Cat. No. E-BC-K030-M), Human Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2) ELISA Kit (Cat No. E-EL-H1564), Human TrxR1 (Thioredoxin Reductase 1) ELISA Kit (Cat. No. E-EL-H1728), human TNF-α ELISA Kit (Cat. No. E-EL-H0109) were from Elabscience, Houston, Tx, USA.

    Techniques: Expressing, Activity Assay, MTT Assay

    Journal: BMC Pharmacology & Toxicology

    Article Title: Epinephrine-mediated sensitization of thioredoxin 1 enhances chlorpromazine protection against acute hepatotoxicity

    doi: 10.1186/s40360-026-01225-w

    Figure Lengend Snippet:

    Article Snippet: Reduced Glutathione (GSH) Colorimetric Assay Kit (Cat. No. E-BC-K030-M), Human Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2) ELISA Kit (Cat No. E-EL-H1564), Human TrxR1 (Thioredoxin Reductase 1) ELISA Kit (Cat. No. E-EL-H1728), human TNF-α ELISA Kit (Cat. No. E-EL-H0109) were from Elabscience, Houston, Tx, USA.

    Techniques:

    Epinephrine and/or chlorpromazine eliminate APAP-induced oxidative stress and inflammation and modulate the expression of the thioredoxin system in the liver. ( A ) and ( B ) demonstrate the hepatic levels of Nrf2 and TNF-α, respectively. ( C ), ( D ), and ( E ) show the effect of EP and CPZ on the thioredoxin system (Trx1/TrxR1) ( C ), thioredoxin-related proteins (TrxL1 and TrxIP) ( D ) mRNA and TrxR1 protein ( E ). Bars represent the mean (± SD) of 3 experiments. Means are compared by two-way ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, *** p < 0.0001, **** p < 0.0001, and “ns” is not significant

    Journal: BMC Pharmacology & Toxicology

    Article Title: Epinephrine-mediated sensitization of thioredoxin 1 enhances chlorpromazine protection against acute hepatotoxicity

    doi: 10.1186/s40360-026-01225-w

    Figure Lengend Snippet: Epinephrine and/or chlorpromazine eliminate APAP-induced oxidative stress and inflammation and modulate the expression of the thioredoxin system in the liver. ( A ) and ( B ) demonstrate the hepatic levels of Nrf2 and TNF-α, respectively. ( C ), ( D ), and ( E ) show the effect of EP and CPZ on the thioredoxin system (Trx1/TrxR1) ( C ), thioredoxin-related proteins (TrxL1 and TrxIP) ( D ) mRNA and TrxR1 protein ( E ). Bars represent the mean (± SD) of 3 experiments. Means are compared by two-way ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, *** p < 0.0001, **** p < 0.0001, and “ns” is not significant

    Article Snippet: Reduced Glutathione (GSH) Colorimetric Assay Kit (Cat. No. E-BC-K030-M), Human Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2) ELISA Kit (Cat No. E-EL-H1564), Human TrxR1 (Thioredoxin Reductase 1) ELISA Kit (Cat. No. E-EL-H1728), human TNF-α ELISA Kit (Cat. No. E-EL-H0109) were from Elabscience, Houston, Tx, USA.

    Techniques: Expressing

    Epinephrine and EGCG effect on cell viability, Nrf2, NTF, and TrxR1 expression in hepatoma cells. HepG2 and Huh7 cells were treated with epinephrine ( A ) or EGCG ( B ) for 24 h, after which cell metabolic activity was determined by MTT assay. Epinephrine enhanced cell viability in both HepG2 and Huh7 cells, whereas EGCG reduced cell viability. ( C ), ( D ), and ( E ) show the protein levels of Nrf2, TNF-α, and TrxR1, respectively, assessed in cells treated with EP, EGCG, CPZ, EP/CPZ, or EGCG/CPZ. Bars represent the mean (± SD) of 3 experiments. Groups were compared using ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, (***): p < 0.001, (****): p < 0.0001, and “ns” is not significant

    Journal: BMC Pharmacology & Toxicology

    Article Title: Epinephrine-mediated sensitization of thioredoxin 1 enhances chlorpromazine protection against acute hepatotoxicity

    doi: 10.1186/s40360-026-01225-w

    Figure Lengend Snippet: Epinephrine and EGCG effect on cell viability, Nrf2, NTF, and TrxR1 expression in hepatoma cells. HepG2 and Huh7 cells were treated with epinephrine ( A ) or EGCG ( B ) for 24 h, after which cell metabolic activity was determined by MTT assay. Epinephrine enhanced cell viability in both HepG2 and Huh7 cells, whereas EGCG reduced cell viability. ( C ), ( D ), and ( E ) show the protein levels of Nrf2, TNF-α, and TrxR1, respectively, assessed in cells treated with EP, EGCG, CPZ, EP/CPZ, or EGCG/CPZ. Bars represent the mean (± SD) of 3 experiments. Groups were compared using ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, (***): p < 0.001, (****): p < 0.0001, and “ns” is not significant

    Article Snippet: Reduced Glutathione (GSH) Colorimetric Assay Kit (Cat. No. E-BC-K030-M), Human Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2) ELISA Kit (Cat No. E-EL-H1564), Human TrxR1 (Thioredoxin Reductase 1) ELISA Kit (Cat. No. E-EL-H1728), human TNF-α ELISA Kit (Cat. No. E-EL-H0109) were from Elabscience, Houston, Tx, USA.

    Techniques: Expressing, Activity Assay, MTT Assay

    HCC cells promote MC migration and sustain MC proliferation. (A, B) Timer3.0 database analysis of SCF expression in tumors and correlation analysis between SCF and MCs; (C) ELISA detection of SCF levels in tumor tissues and adjacent tissues; (D) Correlation analysis between TAMC density and SCF concentration in HCC tumor tissues; (E): Flow cytometry identification of expanded hMCs at week 4; (F, G) Transwell assay for hMC migration capacity; (H, I) Flow cytometry for hMC proliferation; (J, K) Flow cytometry for hMC cell cycle. Data are presented as mean ± SD. Figure (B) used independent samples t ‐tests ( n = 59). (D) Spearman 's rank correlation analysis ( n = 59); (G/I/K), one‐way ANOVA with Tukey's post‐hoc multiple comparison test ( N = 3). * indicates p < 0.05; *** indicates p < 0.001.

    Journal: Journal of Biochemical and Molecular Toxicology

    Article Title: Tumor‐Associated Mast Cells Drive Immunosuppression and Resistance to Anti‐PD‐1 Therapy in Hepatocellular Carcinoma Through CCL17/CCR4‐mediated Regulatory T‐Cell Recruitment

    doi: 10.1002/jbt.71127

    Figure Lengend Snippet: HCC cells promote MC migration and sustain MC proliferation. (A, B) Timer3.0 database analysis of SCF expression in tumors and correlation analysis between SCF and MCs; (C) ELISA detection of SCF levels in tumor tissues and adjacent tissues; (D) Correlation analysis between TAMC density and SCF concentration in HCC tumor tissues; (E): Flow cytometry identification of expanded hMCs at week 4; (F, G) Transwell assay for hMC migration capacity; (H, I) Flow cytometry for hMC proliferation; (J, K) Flow cytometry for hMC cell cycle. Data are presented as mean ± SD. Figure (B) used independent samples t ‐tests ( n = 59). (D) Spearman 's rank correlation analysis ( n = 59); (G/I/K), one‐way ANOVA with Tukey's post‐hoc multiple comparison test ( N = 3). * indicates p < 0.05; *** indicates p < 0.001.

    Article Snippet: The following kits were utilized: human SCF ELISA Kit (E‐EL‐H1299, Elabscience), human CCL1 ELISA kit (ab314600, Abcam), human CCL5 ELISA kit (ab174446, Abcam), human CCL17 ELISA kit (ab183366, Abcam), Human CCL22 ELISA Kit (ab242931, Abcam), Human CCL28 ELISA Kit (ab99988, Abcam), Human Granzyme B ELISA Kit (E‐EL‐H1617, Elabscience), Human Interferon‐γ (IFN‐γ) ELISA Kit (E‐EL‐H0108, Elabscience), Human TNF‐α ELISA Kit (E‐EL‐H0109, Elabscience), Human Perforin 1 ELISA Kit (E‐EL‐H1123, Elabscience), Mouse Granzyme B ELISA Kit (E‐EL‐M0594, Elabscience), Mouse IFN‐γ ELISA Kit (E‐EL‐M0048, Elabscience), Mouse TNF‐α ELISA Kit (E‐EL‐M3063, Elabscience), and Mouse Perforin ELISA Kit (E‐EL‐M0890, Elabscience).

    Techniques: Migration, Analysis, Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay, Flow Cytometry, Transwell Assay, Comparison, X-Ray Photoelectron Spectroscopy

    TAMCs recruit Tregs into the tumor microenvironment by secreting chemokines. (A) ELISA detection of CCL1, CCL5, CCL17, CCL22, and CCL28 secretion by TAMCs (after subtracting CCL1, CCL5, CCL17, CCL22, and CCL28 levels in TCCS); (B) ELISA detection of CCL17 levels in tumor tissues and adjacent tissues; (C) Correlation analysis between TAMCs and CCL17 concentration; D: Correlation analysis between Tregs and CCL17 concentration; (E, F) Transwell migration assay to assess Treg migration capacity. Data are presented as mean ± SD. (A, B), independent samples t ‐tests ( N = 3); (C, D) Spearman 's rank correlation analysis ( n = 59); (F), one‐way ANOVA and Tukey's multiple comparison test ( N = 3). * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001.

    Journal: Journal of Biochemical and Molecular Toxicology

    Article Title: Tumor‐Associated Mast Cells Drive Immunosuppression and Resistance to Anti‐PD‐1 Therapy in Hepatocellular Carcinoma Through CCL17/CCR4‐mediated Regulatory T‐Cell Recruitment

    doi: 10.1002/jbt.71127

    Figure Lengend Snippet: TAMCs recruit Tregs into the tumor microenvironment by secreting chemokines. (A) ELISA detection of CCL1, CCL5, CCL17, CCL22, and CCL28 secretion by TAMCs (after subtracting CCL1, CCL5, CCL17, CCL22, and CCL28 levels in TCCS); (B) ELISA detection of CCL17 levels in tumor tissues and adjacent tissues; (C) Correlation analysis between TAMCs and CCL17 concentration; D: Correlation analysis between Tregs and CCL17 concentration; (E, F) Transwell migration assay to assess Treg migration capacity. Data are presented as mean ± SD. (A, B), independent samples t ‐tests ( N = 3); (C, D) Spearman 's rank correlation analysis ( n = 59); (F), one‐way ANOVA and Tukey's multiple comparison test ( N = 3). * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001.

    Article Snippet: The following kits were utilized: human SCF ELISA Kit (E‐EL‐H1299, Elabscience), human CCL1 ELISA kit (ab314600, Abcam), human CCL5 ELISA kit (ab174446, Abcam), human CCL17 ELISA kit (ab183366, Abcam), Human CCL22 ELISA Kit (ab242931, Abcam), Human CCL28 ELISA Kit (ab99988, Abcam), Human Granzyme B ELISA Kit (E‐EL‐H1617, Elabscience), Human Interferon‐γ (IFN‐γ) ELISA Kit (E‐EL‐H0108, Elabscience), Human TNF‐α ELISA Kit (E‐EL‐H0109, Elabscience), Human Perforin 1 ELISA Kit (E‐EL‐H1123, Elabscience), Mouse Granzyme B ELISA Kit (E‐EL‐M0594, Elabscience), Mouse IFN‐γ ELISA Kit (E‐EL‐M0048, Elabscience), Mouse TNF‐α ELISA Kit (E‐EL‐M3063, Elabscience), and Mouse Perforin ELISA Kit (E‐EL‐M0890, Elabscience).

    Techniques: Enzyme-linked Immunosorbent Assay, Analysis, Concentration Assay, Transwell Migration Assay, Migration, Comparison

    The TAMCs‐Tregs axis inhibits CD8 + T cell function. (A, B) Flow cytometry analysis of Treg‐mediated suppression of CD8 + T cell proliferation; (C–F) ELISA for Granzyme B, IFN‐γ, TNF‐α, and Perforin in supernatants from CD8 + T cell proliferation assays. Data are presented as mean ± SD. One‐way ANOVA was performed, followed by Tukey's multiple comparison test for post‐hoc analysis ( N = 3). * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001.

    Journal: Journal of Biochemical and Molecular Toxicology

    Article Title: Tumor‐Associated Mast Cells Drive Immunosuppression and Resistance to Anti‐PD‐1 Therapy in Hepatocellular Carcinoma Through CCL17/CCR4‐mediated Regulatory T‐Cell Recruitment

    doi: 10.1002/jbt.71127

    Figure Lengend Snippet: The TAMCs‐Tregs axis inhibits CD8 + T cell function. (A, B) Flow cytometry analysis of Treg‐mediated suppression of CD8 + T cell proliferation; (C–F) ELISA for Granzyme B, IFN‐γ, TNF‐α, and Perforin in supernatants from CD8 + T cell proliferation assays. Data are presented as mean ± SD. One‐way ANOVA was performed, followed by Tukey's multiple comparison test for post‐hoc analysis ( N = 3). * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001.

    Article Snippet: The following kits were utilized: human SCF ELISA Kit (E‐EL‐H1299, Elabscience), human CCL1 ELISA kit (ab314600, Abcam), human CCL5 ELISA kit (ab174446, Abcam), human CCL17 ELISA kit (ab183366, Abcam), Human CCL22 ELISA Kit (ab242931, Abcam), Human CCL28 ELISA Kit (ab99988, Abcam), Human Granzyme B ELISA Kit (E‐EL‐H1617, Elabscience), Human Interferon‐γ (IFN‐γ) ELISA Kit (E‐EL‐H0108, Elabscience), Human TNF‐α ELISA Kit (E‐EL‐H0109, Elabscience), Human Perforin 1 ELISA Kit (E‐EL‐H1123, Elabscience), Mouse Granzyme B ELISA Kit (E‐EL‐M0594, Elabscience), Mouse IFN‐γ ELISA Kit (E‐EL‐M0048, Elabscience), Mouse TNF‐α ELISA Kit (E‐EL‐M3063, Elabscience), and Mouse Perforin ELISA Kit (E‐EL‐M0890, Elabscience).

    Techniques: Cell Function Assay, Flow Cytometry, Analysis, Enzyme-linked Immunosorbent Assay, Comparison

    Combination therapy of anti‐PD‐1 antibody and CCR4 antagonists reshapes the tumor immune microenvironment in HCC. (A–F) Flow cytometry analysis of immune cell proportions (MCs, Tregs, CD8 + T cells) in tumor tissue; G–K: ELISA detection of Granzyme B, IFN‐γ, TNF‐α, Perforin, and CCL17 levels. Data are presented as mean ± SD. One‐way ANOVA was performed, followed by Tukey's multiple comparison test for post‐hoc analysis ( n = 6). * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001.

    Journal: Journal of Biochemical and Molecular Toxicology

    Article Title: Tumor‐Associated Mast Cells Drive Immunosuppression and Resistance to Anti‐PD‐1 Therapy in Hepatocellular Carcinoma Through CCL17/CCR4‐mediated Regulatory T‐Cell Recruitment

    doi: 10.1002/jbt.71127

    Figure Lengend Snippet: Combination therapy of anti‐PD‐1 antibody and CCR4 antagonists reshapes the tumor immune microenvironment in HCC. (A–F) Flow cytometry analysis of immune cell proportions (MCs, Tregs, CD8 + T cells) in tumor tissue; G–K: ELISA detection of Granzyme B, IFN‐γ, TNF‐α, Perforin, and CCL17 levels. Data are presented as mean ± SD. One‐way ANOVA was performed, followed by Tukey's multiple comparison test for post‐hoc analysis ( n = 6). * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001.

    Article Snippet: The following kits were utilized: human SCF ELISA Kit (E‐EL‐H1299, Elabscience), human CCL1 ELISA kit (ab314600, Abcam), human CCL5 ELISA kit (ab174446, Abcam), human CCL17 ELISA kit (ab183366, Abcam), Human CCL22 ELISA Kit (ab242931, Abcam), Human CCL28 ELISA Kit (ab99988, Abcam), Human Granzyme B ELISA Kit (E‐EL‐H1617, Elabscience), Human Interferon‐γ (IFN‐γ) ELISA Kit (E‐EL‐H0108, Elabscience), Human TNF‐α ELISA Kit (E‐EL‐H0109, Elabscience), Human Perforin 1 ELISA Kit (E‐EL‐H1123, Elabscience), Mouse Granzyme B ELISA Kit (E‐EL‐M0594, Elabscience), Mouse IFN‐γ ELISA Kit (E‐EL‐M0048, Elabscience), Mouse TNF‐α ELISA Kit (E‐EL‐M3063, Elabscience), and Mouse Perforin ELISA Kit (E‐EL‐M0890, Elabscience).

    Techniques: Flow Cytometry, Analysis, Immune Cell, Tissue, Enzyme-linked Immunosorbent Assay, Comparison

    Validation of the synergistic antitumor efficacy of the triple‐drug therapy in an in situ Hepa1‐6 HCC model. (A) Representative images of in situ tumors; (B): Tumor volume in each group; (C) Tumor weight in each group; (D) Average ratio of liver weight to body weight; (E, F): Ki67 immunohistochemical staining and quantitative analysis; (G–L) Flow cytometry detection of the proportions of various immune cells (mast cells, Tregs, CD8 + T cells); (M) ELISA detection of Granzyme B, IFN‐γ, TNF‐α, and perforin levels. Data are presented as mean ± SD. One‐way ANOVA was performed, followed by Tukey's multiple comparison test for post‐hoc analysis ( n = 6).

    Journal: Journal of Biochemical and Molecular Toxicology

    Article Title: Tumor‐Associated Mast Cells Drive Immunosuppression and Resistance to Anti‐PD‐1 Therapy in Hepatocellular Carcinoma Through CCL17/CCR4‐mediated Regulatory T‐Cell Recruitment

    doi: 10.1002/jbt.71127

    Figure Lengend Snippet: Validation of the synergistic antitumor efficacy of the triple‐drug therapy in an in situ Hepa1‐6 HCC model. (A) Representative images of in situ tumors; (B): Tumor volume in each group; (C) Tumor weight in each group; (D) Average ratio of liver weight to body weight; (E, F): Ki67 immunohistochemical staining and quantitative analysis; (G–L) Flow cytometry detection of the proportions of various immune cells (mast cells, Tregs, CD8 + T cells); (M) ELISA detection of Granzyme B, IFN‐γ, TNF‐α, and perforin levels. Data are presented as mean ± SD. One‐way ANOVA was performed, followed by Tukey's multiple comparison test for post‐hoc analysis ( n = 6).

    Article Snippet: The following kits were utilized: human SCF ELISA Kit (E‐EL‐H1299, Elabscience), human CCL1 ELISA kit (ab314600, Abcam), human CCL5 ELISA kit (ab174446, Abcam), human CCL17 ELISA kit (ab183366, Abcam), Human CCL22 ELISA Kit (ab242931, Abcam), Human CCL28 ELISA Kit (ab99988, Abcam), Human Granzyme B ELISA Kit (E‐EL‐H1617, Elabscience), Human Interferon‐γ (IFN‐γ) ELISA Kit (E‐EL‐H0108, Elabscience), Human TNF‐α ELISA Kit (E‐EL‐H0109, Elabscience), Human Perforin 1 ELISA Kit (E‐EL‐H1123, Elabscience), Mouse Granzyme B ELISA Kit (E‐EL‐M0594, Elabscience), Mouse IFN‐γ ELISA Kit (E‐EL‐M0048, Elabscience), Mouse TNF‐α ELISA Kit (E‐EL‐M3063, Elabscience), and Mouse Perforin ELISA Kit (E‐EL‐M0890, Elabscience).

    Techniques: Biomarker Discovery, In Situ, Immunohistochemical staining, Staining, Analysis, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Comparison